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The map label for this gene is pucI [H]

Identifier: 119870985

GI number: 119870985

Start: 5197189

End: 5198745

Strand: Direct

Name: pucI [H]

Synonym: Mkms_4958

Alternate gene names: 119870985

Gene position: 5197189-5198745 (Clockwise)

Preceding gene: 119870984

Following gene: 119870986

Centisome position: 90.59

GC content: 66.54

Gene sequence:

>1557_bases
ATGACAGACACCCGTGACCTCCCGCCGAGCGCCGTGGTCGGCGCGGGCGACATCGTCGAAGCCGCAGGGCATCCCGTCGG
CAGCGGGGTGATCAAGGACAGCTACGACCCGCGGCTGACCAACGAGGACCTCGCACCCCTGGGCAAGCAGACGTGGTCGT
CGTACAACATCTTCGCGTTCTGGATGTCGGACGTGCACAGCGTCGGCGGATATGTCACCGCGGGCAGCCTGTTCGCCCTG
GGCCTGGCGAGCTGGCAGGTGCTGATCGCCCTGCTCGTCGGCATCGTGATCGTCAACCTGCTGTGCAACCTGGTCGCCAA
GCCCAGCCAGCAGGCCGGCGTGCCGTACCCCGTCGTATGCCGCAGTTCCTTCGGGGTCCTCGGCGCGAACATCCCGGCCA
TCATCCGCGGCCTGATCGCGGTGGCCTGGTACGGCATCCAGACCTACCTGGCGTCGGCGGCGCTCGACGTCGTACTGCTC
AAACTGTTCCCCGGCCTGGCGCCCTACGCCGACGCCGACCAGTACGGCTTCACCGGCCTGTCCCTGCTGGGCTGGTGCAG
CTTCATGCTGCTGTGGGTTCTGCAGGCGTGCGTGTTCTGGCGCGGTATGGAGTCGATCCGCAAGTTCATCGACTTCTGCG
GTCCCGCGGTGTACGTGGTGATGTTCATCCTCTGCGGCTACCTGCTGTGGAAGTCGGGCTGGCACGTCAGCCTGTCGCTG
GGCGGCGAGAAGCAGGGCAACACGCTGGTGGTCATGCTCGGCGCGATCGCGCTCGTCGTGTCGTACTTCTCCGGGCCGAT
GCTGAACTTCGGCGACTTCGCCCGCTACGGCAAGAGCTTCGAGGCGGTCAAGAAGGGCAACTTCCTCGGCCTGCCGATCA
ACTTCCTGATGTTCTCGATCCTGGTCGTCGTCACCGCCGCAGCCACGGTGCCGGTGTTCGGCGAGCTCCTCACCGACCCG
GTCGAGACCGTCGCCCGCATCGACAGCGTCACCGCGATCGTCCTCGGAGCGCTGACGTTCTCGATCGCCACGATCGGCAT
CAACATCGTGGCCAACTTCATCAGCCCCGCCTTCGACTTCTCCAACGTCAGCCCGCAGCGGATCAGCTGGCGCATGGGCG
GCATGATCGCCGCGGTCGGGTCGGTGCTGCTCACGCCGTGGAACCTCTACAGCAACCCCGAGGTCATCCACTACACGCTG
GAGACCCTCGGTGCGTTCATCGGCCCGCTGTTCGGTGTGCTGATCGCCGACTTCTACCTGGTGCGCAAGCAGAAGATCGT
GGTCGACGATCTGTTCACGATGTCGGAGACCGCCAACTACTGGTACCGGAGGGGCTACAACCCCGCCGCGGTGACCGCCA
CTCTGGTCGGCGCCGTCCTGGCCATGGCACCGGTACTGCTCGGCGGCGTCGTACTCGGCATGGCCGGCGCCGCGCAGTAC
AGCTGGTTCATCGGCTGCGGTGTGGCGTTCGCCCTCTACTACGTGCTGGCCACCCGCGGCCCGTGGCGCATGACCGCGCT
GCGCGTGGCCGAGGGCGCGACGCTGGTCTCGAACTAG

Upstream 100 bases:

>100_bases
TGTTGCCGAACGCGCTCAAATATATTTGCGGCGCAACGACGGTGAAACACACCGCAGCTACGTTCCGTTCATCGCCACGC
CGATGGACAGGAACCGCACC

Downstream 100 bases:

>100_bases
GCGACACCTGCGGCCCGACATTCCACATCTGGAATGTCGGGCCGTACGCTTTCGTGGTGAGCCTCCGATTCGCCGCCCTC
GGCCTCCTCGCCCAGCATCC

Product: cytosine/purines, uracil, thiamine, allantoin permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTDTRDLPPSAVVGAGDIVEAAGHPVGSGVIKDSYDPRLTNEDLAPLGKQTWSSYNIFAFWMSDVHSVGGYVTAGSLFAL
GLASWQVLIALLVGIVIVNLLCNLVAKPSQQAGVPYPVVCRSSFGVLGANIPAIIRGLIAVAWYGIQTYLASAALDVVLL
KLFPGLAPYADADQYGFTGLSLLGWCSFMLLWVLQACVFWRGMESIRKFIDFCGPAVYVVMFILCGYLLWKSGWHVSLSL
GGEKQGNTLVVMLGAIALVVSYFSGPMLNFGDFARYGKSFEAVKKGNFLGLPINFLMFSILVVVTAAATVPVFGELLTDP
VETVARIDSVTAIVLGALTFSIATIGINIVANFISPAFDFSNVSPQRISWRMGGMIAAVGSVLLTPWNLYSNPEVIHYTL
ETLGAFIGPLFGVLIADFYLVRKQKIVVDDLFTMSETANYWYRRGYNPAAVTATLVGAVLAMAPVLLGGVVLGMAGAAQY
SWFIGCGVAFALYYVLATRGPWRMTALRVAEGATLVSN

Sequences:

>Translated_518_residues
MTDTRDLPPSAVVGAGDIVEAAGHPVGSGVIKDSYDPRLTNEDLAPLGKQTWSSYNIFAFWMSDVHSVGGYVTAGSLFAL
GLASWQVLIALLVGIVIVNLLCNLVAKPSQQAGVPYPVVCRSSFGVLGANIPAIIRGLIAVAWYGIQTYLASAALDVVLL
KLFPGLAPYADADQYGFTGLSLLGWCSFMLLWVLQACVFWRGMESIRKFIDFCGPAVYVVMFILCGYLLWKSGWHVSLSL
GGEKQGNTLVVMLGAIALVVSYFSGPMLNFGDFARYGKSFEAVKKGNFLGLPINFLMFSILVVVTAAATVPVFGELLTDP
VETVARIDSVTAIVLGALTFSIATIGINIVANFISPAFDFSNVSPQRISWRMGGMIAAVGSVLLTPWNLYSNPEVIHYTL
ETLGAFIGPLFGVLIADFYLVRKQKIVVDDLFTMSETANYWYRRGYNPAAVTATLVGAVLAMAPVLLGGVVLGMAGAAQY
SWFIGCGVAFALYYVLATRGPWRMTALRVAEGATLVSN
>Mature_517_residues
TDTRDLPPSAVVGAGDIVEAAGHPVGSGVIKDSYDPRLTNEDLAPLGKQTWSSYNIFAFWMSDVHSVGGYVTAGSLFALG
LASWQVLIALLVGIVIVNLLCNLVAKPSQQAGVPYPVVCRSSFGVLGANIPAIIRGLIAVAWYGIQTYLASAALDVVLLK
LFPGLAPYADADQYGFTGLSLLGWCSFMLLWVLQACVFWRGMESIRKFIDFCGPAVYVVMFILCGYLLWKSGWHVSLSLG
GEKQGNTLVVMLGAIALVVSYFSGPMLNFGDFARYGKSFEAVKKGNFLGLPINFLMFSILVVVTAAATVPVFGELLTDPV
ETVARIDSVTAIVLGALTFSIATIGINIVANFISPAFDFSNVSPQRISWRMGGMIAAVGSVLLTPWNLYSNPEVIHYTLE
TLGAFIGPLFGVLIADFYLVRKQKIVVDDLFTMSETANYWYRRGYNPAAVTATLVGAVLAMAPVLLGGVVLGMAGAAQYS
WFIGCGVAFALYYVLATRGPWRMTALRVAEGATLVSN

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=499, Percent_Identity=28.6573146292585, Blast_Score=208, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6319495, Length=493, Percent_Identity=24.3407707910751, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6322219, Length=468, Percent_Identity=23.2905982905983, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319429, Length=457, Percent_Identity=24.945295404814, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6324766, Length=445, Percent_Identity=22.4719101123595, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323266, Length=445, Percent_Identity=22.6966292134831, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324645, Length=451, Percent_Identity=22.3946784922395, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 55516; Mature: 55384

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTRDLPPSAVVGAGDIVEAAGHPVGSGVIKDSYDPRLTNEDLAPLGKQTWSSYNIFAF
CCCCCCCCCCHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHCCCCCEEEH
WMSDVHSVGGYVTAGSLFALGLASWQVLIALLVGIVIVNLLCNLVAKPSQQAGVPYPVVC
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
RSSFGVLGANIPAIIRGLIAVAWYGIQTYLASAALDVVLLKLFPGLAPYADADQYGFTGL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
SLLGWCSFMLLWVLQACVFWRGMESIRKFIDFCGPAVYVVMFILCGYLLWKSGWHVSLSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEE
GGEKQGNTLVVMLGAIALVVSYFSGPMLNFGDFARYGKSFEAVKKGNFLGLPINFLMFSI
CCCCCCCEEEHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHCCCEECCCHHHHHHHH
LVVVTAAATVPVFGELLTDPVETVARIDSVTAIVLGALTFSIATIGINIVANFISPAFDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SNVSPQRISWRMGGMIAAVGSVLLTPWNLYSNPEVIHYTLETLGAFIGPLFGVLIADFYL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
VRKQKIVVDDLFTMSETANYWYRRGYNPAAVTATLVGAVLAMAPVLLGGVVLGMAGAAQY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
SWFIGCGVAFALYYVLATRGPWRMTALRVAEGATLVSN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
TDTRDLPPSAVVGAGDIVEAAGHPVGSGVIKDSYDPRLTNEDLAPLGKQTWSSYNIFAF
CCCCCCCCCHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHCCCCCEEEH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HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]