Click here to switch to the map view.

The map label for this gene is otsB [H]

Identifier: 119870828

GI number: 119870828

Start: 5032549

End: 5033319

Strand: Direct

Name: otsB [H]

Synonym: Mkms_4800

Alternate gene names: 119870828

Gene position: 5032549-5033319 (Clockwise)

Preceding gene: 119870827

Following gene: 119870829

Centisome position: 87.72

GC content: 70.17

Gene sequence:

>771_bases
GTGGCCGACGGCGTACCGACAGATCTCCGCCAGGCGCTCGACGGCGCCGCACGACTTCCGCGTCTGCTGATCGCCTGTGA
CTATGACGGCACGCTGGCGCCGATCGTGTCCAACCCCGCCGACGCCCGTCCGCTGCCCGCGTCGGCGGCCGCGGTGGAGG
AACTGGCTGCGCTGCCCGCCACCACGGTCGCGCTGATCTCCGGCCGCGCACTGGGCGTGCTCAAGGAGCTCTCCGGGGTG
TCCGGCCGTGTGCACCTGGTCGGCAGCCACGGAGCGGAGTTCGACACCGGGTTCCTGTCCCCCATCGATGAGCGGGCCGA
AGCACTGCTGGTCGAGATCAAACAGACCCTCGATGAGATCGCCGCCGAATACGCCGGTGTGACAACCGAACTCAAACCCG
CCAGTGTCGCGCTGCACGTGCGCAACGCCTCGACCGACGACGGTGAAGCGGCGATGCACCGCGCGAACGAGGCGGCCGCG
CGGTGGGATGCGCAGGTCACCGACGGCAAGGCGGTCAAGGAGTTCGCCGTCATCCAGACCGACAAGGGACAGGCCGTCGA
CATCCTGCGCGATCAGCACGACGCCTCGGCGGTGCTGTTCCTCGGCGACGACGTCACCGACGAGAAGGCGTTCCGCCGGA
TGCGCGACGGCGACATCGGTGTGAAGGTCGGCCCCGGCGACACCGCGGCGGCGTACCGGGTCGACGAACCCCGCGACGTG
GCCGAGGTGCTCGAGTACCTGCTGGCCGGGCGGCGTCGTGCTCACTCCTGA

Upstream 100 bases:

>100_bases
TGGCTGACCCGCCGCGCCTCGGCGACCGACGTGAGCAAACGGCTCGATTTGGCGGTGCGGCTGCTCATCGGCGACGGCGA
CCGCCCTAAGATCTGAACGC

Downstream 100 bases:

>100_bases
CGAGCTGACCGCGCGGACCGCGCGGGCCGTCGCGGCGGCGGCCTCCGCGGGACGCGACCTCGGTCTGCACGTCGACGAGC
CGCGTGTGCTCTACGACGTC

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose-6-phosphate phosphatase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MADGVPTDLRQALDGAARLPRLLIACDYDGTLAPIVSNPADARPLPASAAAVEELAALPATTVALISGRALGVLKELSGV
SGRVHLVGSHGAEFDTGFLSPIDERAEALLVEIKQTLDEIAAEYAGVTTELKPASVALHVRNASTDDGEAAMHRANEAAA
RWDAQVTDGKAVKEFAVIQTDKGQAVDILRDQHDASAVLFLGDDVTDEKAFRRMRDGDIGVKVGPGDTAAAYRVDEPRDV
AEVLEYLLAGRRRAHS

Sequences:

>Translated_256_residues
MADGVPTDLRQALDGAARLPRLLIACDYDGTLAPIVSNPADARPLPASAAAVEELAALPATTVALISGRALGVLKELSGV
SGRVHLVGSHGAEFDTGFLSPIDERAEALLVEIKQTLDEIAAEYAGVTTELKPASVALHVRNASTDDGEAAMHRANEAAA
RWDAQVTDGKAVKEFAVIQTDKGQAVDILRDQHDASAVLFLGDDVTDEKAFRRMRDGDIGVKVGPGDTAAAYRVDEPRDV
AEVLEYLLAGRRRAHS
>Mature_255_residues
ADGVPTDLRQALDGAARLPRLLIACDYDGTLAPIVSNPADARPLPASAAAVEELAALPATTVALISGRALGVLKELSGVS
GRVHLVGSHGAEFDTGFLSPIDERAEALLVEIKQTLDEIAAEYAGVTTELKPASVALHVRNASTDDGEAAMHRANEAAAR
WDAQVTDGKAVKEFAVIQTDKGQAVDILRDQHDASAVLFLGDDVTDEKAFRRMRDGDIGVKVGPGDTAAAYRVDEPRDVA
EVLEYLLAGRRRAHS

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=227, Percent_Identity=33.4801762114537, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320279, Length=230, Percent_Identity=27.3913043478261, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19920676, Length=254, Percent_Identity=27.1653543307087, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI221473368, Length=225, Percent_Identity=28.4444444444444, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI20129307, Length=225, Percent_Identity=28.4444444444444, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24582490, Length=225, Percent_Identity=28.4444444444444, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 27010; Mature: 26878

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADGVPTDLRQALDGAARLPRLLIACDYDGTLAPIVSNPADARPLPASAAAVEELAALPA
CCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCH
TTVALISGRALGVLKELSGVSGRVHLVGSHGAEFDTGFLSPIDERAEALLVEIKQTLDEI
HHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AAEYAGVTTELKPASVALHVRNASTDDGEAAMHRANEAAARWDAQVTDGKAVKEFAVIQT
HHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEEC
DKGQAVDILRDQHDASAVLFLGDDVTDEKAFRRMRDGDIGVKVGPGDTAAAYRVDEPRDV
CCCCEEHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCEEEECCCCHHH
AEVLEYLLAGRRRAHS
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADGVPTDLRQALDGAARLPRLLIACDYDGTLAPIVSNPADARPLPASAAAVEELAALPA
CCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCH
TTVALISGRALGVLKELSGVSGRVHLVGSHGAEFDTGFLSPIDERAEALLVEIKQTLDEI
HHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AAEYAGVTTELKPASVALHVRNASTDDGEAAMHRANEAAARWDAQVTDGKAVKEFAVIQT
HHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEEC
DKGQAVDILRDQHDASAVLFLGDDVTDEKAFRRMRDGDIGVKVGPGDTAAAYRVDEPRDV
CCCCEEHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCEEEECCCCHHH
AEVLEYLLAGRRRAHS
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8446027; 11234002 [H]