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The map label for this gene is uxuB [H]

Identifier: 119870474

GI number: 119870474

Start: 4678601

End: 4680019

Strand: Direct

Name: uxuB [H]

Synonym: Mkms_4445

Alternate gene names: 119870474

Gene position: 4678601-4680019 (Clockwise)

Preceding gene: 119870468

Following gene: 119870475

Centisome position: 81.55

GC content: 66.46

Gene sequence:

>1419_bases
ATGAAACTCGACGAGTCGACCCTGCCCGACATCCCCATCGCCAAGCCGACCTACGCACGCGGCGATGTCACCGTCGGCAT
CGTGCACTTCGGTGTCGGCGGCTTCCACCGCGCACACCAGGCCATGTACGTCGACGCACTCCTCGAGAAGGGCCTCGCCG
CCGAGTGGGGCATCTGCGGGGTCGGCGTGCTACCCGGTGACCGCAAGATGGCCGATGCGCTGGGCGCCCAGGACGGGCTC
TACACGCTGCTGCTGGAGAATCCGGACGGGAGCCGCGACGCCCGGGTGATCGGATCCATCGTCGACTATCGCTACGCGCC
AGACGATCCCGAGGGCGTCGTCGAACTGCTGGCCGCCCCGAGCACCCGCATCATCTCGCTGACCATCACCGAGGGCGGGT
ACGAGGTGGACAAGGTGGACGCCGATTCCGTCAACGTCTTCGGTCTGATCACCGATGCGCTCGCGCTTCGGCGGGACCGC
GGGGTCGACCCGCCGACGATCGTGTCGTGCGACAACATCGAGGGCAACGGCGACGTGGCCCGCGCGGCGTTCATGTCCTA
TGCCGACCGGACCCATCCAGGCCTGTCCGAGTGGATTGGCTCCAACGCGCGCTTCCCCAACTCGATGGTCGACCGCATCA
CCCCGGTGACCACTCCGGAGGTGATCGCCACCGTACGAGACGAATTCGGTGTCGACGACGCGTGGCCGGTGGTCGCCGAA
CCCTTCACCTCATGGGTGCTCGAGGACACCTTCACCGACGGTCGGCCACCGTTCGAGGACGTCGGCGTGCTGCTGGTCGA
CGACGTCACCCCGTACGAACTGATGAAGTTGCGACTGCTCAACGCCAGTCACCAGGCACTGTGCTATTTCGGCTACCTGG
CCGGCTACCGGCTCGTGCACGACGCCGCGGCCGATCCGCTGTTCGCCGAACTGCTGCGCCGGTACATGGATTCCGAGGCG
ACGCCGACACTACGGCCGGTGCCCGGTATCGACCTGCCCGACTACAAGCGCACTCTCATCGAACGTTTCGCCAATCCCGG
TGTGCGCGACACCATCGCACGGCTGTGTTACGGCTCGTCCGACCGGATCCCGAAGTGGCTGCTTCCGGTGGTGCGGGAGA
ATTTGCGGACCGGCGCACCGATCCGGTTGTCGGCCGCCGTCGTCGCGAGTTGGTCGCGGTACGCCGAGGGTGTCGACGAG
CAGGGTGAGCCGATCGACGTACAGGATCAGCTCGCCGACGAGTTGGTACCGCTGGCGCGCGCCCAACGCGACAACCCGAC
GTCGTTCATCGAGAACCGCTTGGTCTTCGGCGATCTCGTCGACGAGAAACGATTCGTCGACGAATACGTGGCGGCGTTGG
AGTCGCTGCACCGGGTCGGCGCCCGCGCCACGCTCGAAGCACTGGTGGGAAAGCCATGA

Upstream 100 bases:

>100_bases
TGCTCACTGGCGCGAGTAGATGCTCATCACGCTAACATGGTGAATGTGACGCAGACAACACTTTTCTGCCCGGTAACTTC
GAGACGATGGTGACGGTGTC

Downstream 100 bases:

>100_bases
GCCTGCACGCGCTGGTCATCGGAGAGGCACTGATCGACATCGTCGAGCGTGACGGTGTGGTCCAGGGTGAACACGTCGGC
GGCAGCCCACTCAACGTCGC

Product: mannitol dehydrogenase domain-containing protein

Products: NA

Alternate protein names: Fructuronate reductase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKLDESTLPDIPIAKPTYARGDVTVGIVHFGVGGFHRAHQAMYVDALLEKGLAAEWGICGVGVLPGDRKMADALGAQDGL
YTLLLENPDGSRDARVIGSIVDYRYAPDDPEGVVELLAAPSTRIISLTITEGGYEVDKVDADSVNVFGLITDALALRRDR
GVDPPTIVSCDNIEGNGDVARAAFMSYADRTHPGLSEWIGSNARFPNSMVDRITPVTTPEVIATVRDEFGVDDAWPVVAE
PFTSWVLEDTFTDGRPPFEDVGVLLVDDVTPYELMKLRLLNASHQALCYFGYLAGYRLVHDAAADPLFAELLRRYMDSEA
TPTLRPVPGIDLPDYKRTLIERFANPGVRDTIARLCYGSSDRIPKWLLPVVRENLRTGAPIRLSAAVVASWSRYAEGVDE
QGEPIDVQDQLADELVPLARAQRDNPTSFIENRLVFGDLVDEKRFVDEYVAALESLHRVGARATLEALVGKP

Sequences:

>Translated_472_residues
MKLDESTLPDIPIAKPTYARGDVTVGIVHFGVGGFHRAHQAMYVDALLEKGLAAEWGICGVGVLPGDRKMADALGAQDGL
YTLLLENPDGSRDARVIGSIVDYRYAPDDPEGVVELLAAPSTRIISLTITEGGYEVDKVDADSVNVFGLITDALALRRDR
GVDPPTIVSCDNIEGNGDVARAAFMSYADRTHPGLSEWIGSNARFPNSMVDRITPVTTPEVIATVRDEFGVDDAWPVVAE
PFTSWVLEDTFTDGRPPFEDVGVLLVDDVTPYELMKLRLLNASHQALCYFGYLAGYRLVHDAAADPLFAELLRRYMDSEA
TPTLRPVPGIDLPDYKRTLIERFANPGVRDTIARLCYGSSDRIPKWLLPVVRENLRTGAPIRLSAAVVASWSRYAEGVDE
QGEPIDVQDQLADELVPLARAQRDNPTSFIENRLVFGDLVDEKRFVDEYVAALESLHRVGARATLEALVGKP
>Mature_472_residues
MKLDESTLPDIPIAKPTYARGDVTVGIVHFGVGGFHRAHQAMYVDALLEKGLAAEWGICGVGVLPGDRKMADALGAQDGL
YTLLLENPDGSRDARVIGSIVDYRYAPDDPEGVVELLAAPSTRIISLTITEGGYEVDKVDADSVNVFGLITDALALRRDR
GVDPPTIVSCDNIEGNGDVARAAFMSYADRTHPGLSEWIGSNARFPNSMVDRITPVTTPEVIATVRDEFGVDDAWPVVAE
PFTSWVLEDTFTDGRPPFEDVGVLLVDDVTPYELMKLRLLNASHQALCYFGYLAGYRLVHDAAADPLFAELLRRYMDSEA
TPTLRPVPGIDLPDYKRTLIERFANPGVRDTIARLCYGSSDRIPKWLLPVVRENLRTGAPIRLSAAVVASWSRYAEGVDE
QGEPIDVQDQLADELVPLARAQRDNPTSFIENRLVFGDLVDEKRFVDEYVAALESLHRVGARATLEALVGKP

Specific function: Hexuronate conversion to KDG. [C]

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790779, Length=481, Percent_Identity=38.8773388773389, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1788497, Length=480, Percent_Identity=36.875, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1787823, Length=472, Percent_Identity=37.9237288135593, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI48994885, Length=405, Percent_Identity=26.6666666666667, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1790028, Length=222, Percent_Identity=26.5765765765766, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324401, Length=489, Percent_Identity=41.3087934560327, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6320765, Length=489, Percent_Identity=41.3087934560327, Blast_Score=378, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: =1.1.1.57 [H]

Molecular weight: Translated: 51693; Mature: 51693

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDESTLPDIPIAKPTYARGDVTVGIVHFGVGGFHRAHQAMYVDALLEKGLAAEWGICG
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEE
VGVLPGDRKMADALGAQDGLYTLLLENPDGSRDARVIGSIVDYRYAPDDPEGVVELLAAP
EECCCCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
STRIISLTITEGGYEVDKVDADSVNVFGLITDALALRRDRGVDPPTIVSCDNIEGNGDVA
CCEEEEEEEECCCCEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHH
RAAFMSYADRTHPGLSEWIGSNARFPNSMVDRITPVTTPEVIATVRDEFGVDDAWPVVAE
HHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHH
PFTSWVLEDTFTDGRPPFEDVGVLLVDDVTPYELMKLRLLNASHQALCYFGYLAGYRLVH
HHHHHHHHHHCCCCCCCHHHCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DAAADPLFAELLRRYMDSEATPTLRPVPGIDLPDYKRTLIERFANPGVRDTIARLCYGSS
HHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCC
DRIPKWLLPVVRENLRTGAPIRLSAAVVASWSRYAEGVDEQGEPIDVQDQLADELVPLAR
CCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
AQRDNPTSFIENRLVFGDLVDEKRFVDEYVAALESLHRVGARATLEALVGKP
HCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLDESTLPDIPIAKPTYARGDVTVGIVHFGVGGFHRAHQAMYVDALLEKGLAAEWGICG
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEE
VGVLPGDRKMADALGAQDGLYTLLLENPDGSRDARVIGSIVDYRYAPDDPEGVVELLAAP
EECCCCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
STRIISLTITEGGYEVDKVDADSVNVFGLITDALALRRDRGVDPPTIVSCDNIEGNGDVA
CCEEEEEEEECCCCEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHH
RAAFMSYADRTHPGLSEWIGSNARFPNSMVDRITPVTTPEVIATVRDEFGVDDAWPVVAE
HHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHH
PFTSWVLEDTFTDGRPPFEDVGVLLVDDVTPYELMKLRLLNASHQALCYFGYLAGYRLVH
HHHHHHHHHHCCCCCCCHHHCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DAAADPLFAELLRRYMDSEATPTLRPVPGIDLPDYKRTLIERFANPGVRDTIARLCYGSS
HHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCC
DRIPKWLLPVVRENLRTGAPIRLSAAVVASWSRYAEGVDEQGEPIDVQDQLADELVPLAR
CCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
AQRDNPTSFIENRLVFGDLVDEKRFVDEYVAALESLHRVGARATLEALVGKP
HCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]