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The map label for this gene is fumC [H]

Identifier: 119870227

GI number: 119870227

Start: 4423331

End: 4424752

Strand: Direct

Name: fumC [H]

Synonym: Mkms_4197

Alternate gene names: 119870227

Gene position: 4423331-4424752 (Clockwise)

Preceding gene: 119870226

Following gene: 119870229

Centisome position: 77.1

GC content: 68.35

Gene sequence:

>1422_bases
ATGACCGCCGACAGCGCTGTCGACCAGAACACCGAGTACCGCATCGAACACGACACCATGGGCGAGGTCCGGGTGCCCAA
GGATGCGCTGTGGCGGGCGCAGACCCAGCGCGCCGTCGAGAACTTCCCGATCTCGGGCCGCGGCCTCGAGCGCACCCAGA
TCCGCGCGCTGGGACTGCTCAAAGGCGCCTGCGCGCAGGTGAACAAGGATCTCGGCCTGCTCGCTCCCGAGAAGGCGGAC
GCGATCATCGCCGCGGCCGCCGAGATCGCCGACGGGCGTCACGACGACCAGTTCCCGATCGACGTGTTCCAGACCGGTTC
GGGCACCAGTTCGAACATGAACTCCAACGAGGTCATCGCCTCGATCGCGGCGGCGAACGGCGTGACCGTCCACCCCAACG
ACGACGTGAACATGTCGCAGAGCTCCAACGACACGTTCCCGACGGCCACCCACATCGCGGCGACGGAAGCCGCTGTGCGC
CACCTGATCCCGGCGCTCGAGGTGCTGCACGAATCGCTGGCCGGCAAGGCGCGGCAGTGGCGCACGGTCGTGAAGTCCGG
GCGCACGCACCTGATGGACGCGGTGCCGGTGACGCTGGGCCAGGAGTTCGGCGGTTACGCCCGTCAGATCGAGGCCGGTA
TCGAGAGGGTCAAGGCGACGCTGCCCCGGCTGGGTGAGCTGGCCATCGGCGGTACCGCCGTCGGCACCGGGCTCAACGCG
CCCGACGGCTTCGGCGCCAAGGTCGTCGAGGTGCTCGTCGACCAGACCGGTCTGGGCGAATTGACCACGGCCAAGGACTC
TTTCGAGGCGCAGGCCGCCCGCGACGGGCTGGTCGAGGCGTCCGGTGCGCTGCGGGTGATCGCGGTGTCGCTGACCAAGA
TCGCCAACGACATCCGTTGGATGGGTTCGGGCCCGCTGACCGGCCTGGCCGAGATCCAGCTGCCGGATCTGCAGCCGGGC
AGCTCGATCATGCCGGGCAAGGTGAATCCCGTTCTGCCCGAAGCCGTCACGCAGGTCGCCGCCCAGGTCGTCGGCAACGA
CGCCGCCGTCGCATGGGGTGGCGCGGCCGGCGCGTTCGAGCTCAACGTGTACATCCCGATGATGGCGCGCAACCTGCTCG
AGTCGTTCAAGCTGCTGACCAACGTGTCGCGGCTGTTCGCCGAGCGGTGCATCGACGGCCTGGTCGCCAACGAGGAGCAC
ATGCGCGAGCAGGCCGAGTCGTCGCCGTCGATCGTGACGCCGCTGAACTCGGCGATCGGCTACGAGGAGGCGGCCAAGGT
CGCCAAGGAAGCGCTCAAGGAGAAGAAGACGATCCGCCAGACGGTCATCGACCGCGGGCTGATCGGCGACAAGCTCTCCG
AGGAGGAACTCGACCGTCGCCTCGACGTGCTGGCGATGGCGAAGGTCAAGCAGGGCAAGTAG

Upstream 100 bases:

>100_bases
CTACCACCGGCTGTCCAAGCTCAACGAGTACTCCGCTGTCGACTTCACCGGCGACAGCAACGCCGCCTATCCCCTGCCGT
AACCGATCAGAAGGGCATCC

Downstream 100 bases:

>100_bases
CGATTTCGGCGTGCGCGGTCGCGCTGGACGCGACTGCGCACGCCGAAATCACGGGACTTTGCGGTCGGTGATCGGGCCGG
CGCGCAGATCCCGCAGCACG

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTADSAVDQNTEYRIEHDTMGEVRVPKDALWRAQTQRAVENFPISGRGLERTQIRALGLLKGACAQVNKDLGLLAPEKAD
AIIAAAAEIADGRHDDQFPIDVFQTGSGTSSNMNSNEVIASIAAANGVTVHPNDDVNMSQSSNDTFPTATHIAATEAAVR
HLIPALEVLHESLAGKARQWRTVVKSGRTHLMDAVPVTLGQEFGGYARQIEAGIERVKATLPRLGELAIGGTAVGTGLNA
PDGFGAKVVEVLVDQTGLGELTTAKDSFEAQAARDGLVEASGALRVIAVSLTKIANDIRWMGSGPLTGLAEIQLPDLQPG
SSIMPGKVNPVLPEAVTQVAAQVVGNDAAVAWGGAAGAFELNVYIPMMARNLLESFKLLTNVSRLFAERCIDGLVANEEH
MREQAESSPSIVTPLNSAIGYEEAAKVAKEALKEKKTIRQTVIDRGLIGDKLSEEELDRRLDVLAMAKVKQGK

Sequences:

>Translated_473_residues
MTADSAVDQNTEYRIEHDTMGEVRVPKDALWRAQTQRAVENFPISGRGLERTQIRALGLLKGACAQVNKDLGLLAPEKAD
AIIAAAAEIADGRHDDQFPIDVFQTGSGTSSNMNSNEVIASIAAANGVTVHPNDDVNMSQSSNDTFPTATHIAATEAAVR
HLIPALEVLHESLAGKARQWRTVVKSGRTHLMDAVPVTLGQEFGGYARQIEAGIERVKATLPRLGELAIGGTAVGTGLNA
PDGFGAKVVEVLVDQTGLGELTTAKDSFEAQAARDGLVEASGALRVIAVSLTKIANDIRWMGSGPLTGLAEIQLPDLQPG
SSIMPGKVNPVLPEAVTQVAAQVVGNDAAVAWGGAAGAFELNVYIPMMARNLLESFKLLTNVSRLFAERCIDGLVANEEH
MREQAESSPSIVTPLNSAIGYEEAAKVAKEALKEKKTIRQTVIDRGLIGDKLSEEELDRRLDVLAMAKVKQGK
>Mature_472_residues
TADSAVDQNTEYRIEHDTMGEVRVPKDALWRAQTQRAVENFPISGRGLERTQIRALGLLKGACAQVNKDLGLLAPEKADA
IIAAAAEIADGRHDDQFPIDVFQTGSGTSSNMNSNEVIASIAAANGVTVHPNDDVNMSQSSNDTFPTATHIAATEAAVRH
LIPALEVLHESLAGKARQWRTVVKSGRTHLMDAVPVTLGQEFGGYARQIEAGIERVKATLPRLGELAIGGTAVGTGLNAP
DGFGAKVVEVLVDQTGLGELTTAKDSFEAQAARDGLVEASGALRVIAVSLTKIANDIRWMGSGPLTGLAEIQLPDLQPGS
SIMPGKVNPVLPEAVTQVAAQVVGNDAAVAWGGAAGAFELNVYIPMMARNLLESFKLLTNVSRLFAERCIDGLVANEEHM
REQAESSPSIVTPLNSAIGYEEAAKVAKEALKEKKTIRQTVIDRGLIGDKLSEEELDRRLDVLAMAKVKQGK

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=459, Percent_Identity=50.9803921568627, Blast_Score=431, Evalue=1e-121,
Organism=Escherichia coli, GI1787896, Length=452, Percent_Identity=49.3362831858407, Blast_Score=413, Evalue=1e-116,
Organism=Escherichia coli, GI87082375, Length=460, Percent_Identity=40.8695652173913, Blast_Score=313, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17553882, Length=455, Percent_Identity=50.1098901098901, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32565146, Length=320, Percent_Identity=51.875, Blast_Score=322, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6324993, Length=458, Percent_Identity=49.7816593886463, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24640179, Length=450, Percent_Identity=48.8888888888889, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24640177, Length=450, Percent_Identity=48.8888888888889, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI78710009, Length=453, Percent_Identity=48.5651214128035, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24662684, Length=457, Percent_Identity=47.2647702407002, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24583245, Length=445, Percent_Identity=40.8988764044944, Blast_Score=335, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 50215; Mature: 50083

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADSAVDQNTEYRIEHDTMGEVRVPKDALWRAQTQRAVENFPISGRGLERTQIRALGLL
CCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KGACAQVNKDLGLLAPEKADAIIAAAAEIADGRHDDQFPIDVFQTGSGTSSNMNSNEVIA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHH
SIAAANGVTVHPNDDVNMSQSSNDTFPTATHIAATEAAVRHLIPALEVLHESLAGKARQW
HHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTVVKSGRTHLMDAVPVTLGQEFGGYARQIEAGIERVKATLPRLGELAIGGTAVGTGLNA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCC
PDGFGAKVVEVLVDQTGLGELTTAKDSFEAQAARDGLVEASGALRVIAVSLTKIANDIRW
CCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
MGSGPLTGLAEIQLPDLQPGSSIMPGKVNPVLPEAVTQVAAQVVGNDAAVAWGGAAGAFE
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEE
LNVYIPMMARNLLESFKLLTNVSRLFAERCIDGLVANEEHMREQAESSPSIVTPLNSAIG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEECCCHHHCC
YEEAAKVAKEALKEKKTIRQTVIDRGLIGDKLSEEELDRRLDVLAMAKVKQGK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TADSAVDQNTEYRIEHDTMGEVRVPKDALWRAQTQRAVENFPISGRGLERTQIRALGLL
CCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KGACAQVNKDLGLLAPEKADAIIAAAAEIADGRHDDQFPIDVFQTGSGTSSNMNSNEVIA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHH
SIAAANGVTVHPNDDVNMSQSSNDTFPTATHIAATEAAVRHLIPALEVLHESLAGKARQW
HHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTVVKSGRTHLMDAVPVTLGQEFGGYARQIEAGIERVKATLPRLGELAIGGTAVGTGLNA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCC
PDGFGAKVVEVLVDQTGLGELTTAKDSFEAQAARDGLVEASGALRVIAVSLTKIANDIRW
CCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
MGSGPLTGLAEIQLPDLQPGSSIMPGKVNPVLPEAVTQVAAQVVGNDAAVAWGGAAGAFE
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEE
LNVYIPMMARNLLESFKLLTNVSRLFAERCIDGLVANEEHMREQAESSPSIVTPLNSAIG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEECCCHHHCC
YEEAAKVAKEALKEKKTIRQTVIDRGLIGDKLSEEELDRRLDVLAMAKVKQGK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]