Click here to switch to the map view.

The map label for this gene is ybjS [C]

Identifier: 119870215

GI number: 119870215

Start: 4412004

End: 4413119

Strand: Direct

Name: ybjS [C]

Synonym: Mkms_4185

Alternate gene names: 119870215

Gene position: 4412004-4413119 (Clockwise)

Preceding gene: 119870214

Following gene: 119870218

Centisome position: 76.9

GC content: 65.77

Gene sequence:

>1116_bases
ATGGCTGATGCAACGCTGACCACAGAACTGGGCCGCGTGCTGGTGACCGGTGGGTCCGGCTTCGTGGGCGCGAATCTCGT
GACCGAACTGCTCGAGCGCGGCCACCACGTGCGTTCGTTCGACCGCGCCCCCTCCCCGCTGCCACCCCATCCGCTGCTGG
AGACGCTCGAAGGCGACATCTGCGATCCGGAGACGGTGGCCGCCGCGGTCGCCGGCGTCGACACCGTCTTCCACACCGCG
GCGATCATCGACCTGATGGGCGGGGCGTCGGTCACCGACGAGTACCGCCGCCGCAGTTTCGCGGTCAACGTCGGAGGCAC
CGAGAACCTCGTGCGCGCGGGCCGCGCCGCCGGGGTGAAGCGGTTCGTCTACACCGCGTCCAACAGCGTGGTGATGGGCG
GACAGCCGATCGTCGCCGGCGACGAGACCCTGCCCTACACCGACCGGTTCAACGACCTCTACACCGAGACCAAGGTGGCC
GCGGAGAAGTACGTCCTGGCGCAGAACGGGATCGACGGAATGCTGACGTGCTCGATCCGGCCGAGCGGCATCTGGGGCCG
GGGCGATCAGACGATGTTCCGCAAGGTGTTCGAATCCGTCCTGGCCGGACACGTCAAGGTTCTCGTCGGGAGCAAGAACG
TCAAACTCGACAACTCGTACGTGCACAACCTCATTCACGGTTTCATCCTCGCCGCCGAACACCTGGTGCCCGGCGGCACG
GCACCCGGTCAGGCGTATTTCATCAACGACGGGGAGCCGGTCAACATGTTCGAGTTCTCCCGCCCGGTCGTGGAGGCGTG
CGGTGAGCGTTATCCGCGGTTGCGGGTGCCGGGCCGGCTCGTGCACTTCGTGATGACGGTCTGGCAGAAATTGCACTTCC
GCTTCGGGCTGCCCAAACCGTTGCTGGAACCGCTGAGCGTGGAACGGATCTATCTCGACAACCACTTCTCGATCGCGAAG
GCGGAACGGGATCTGGGCTACCGCCCGCTGTTCACCACCGAGCAGGCGATGCAAGCCTGCCTGCCGTATTACGTCGAGCT
GTACGAGAAGATGAAGGCCGACGGAGCGAAAACCCCTGCCGTACAAGCGGTGCGGACCACACCGCCGGCGGGTTGA

Upstream 100 bases:

>100_bases
ATCGGCGCTCGGCGAGCGGGACGCCGACGACGGTTGACTCGGCAACCGAAATCGGACCAAGGGGTTCTGAACTAGAACAC
GTTTCAGTTATAGTCGCGCC

Downstream 100 bases:

>100_bases
TCCCGCCGGTCAGCTCGCCCCGGTGTCGGCGGTCAGATCGCCGTCGTCGACGAACTCCAGACCACCGTCGTCGAGTGAGA
CCGCCCAGCGGCGCGCGGCG

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MADATLTTELGRVLVTGGSGFVGANLVTELLERGHHVRSFDRAPSPLPPHPLLETLEGDICDPETVAAAVAGVDTVFHTA
AIIDLMGGASVTDEYRRRSFAVNVGGTENLVRAGRAAGVKRFVYTASNSVVMGGQPIVAGDETLPYTDRFNDLYTETKVA
AEKYVLAQNGIDGMLTCSIRPSGIWGRGDQTMFRKVFESVLAGHVKVLVGSKNVKLDNSYVHNLIHGFILAAEHLVPGGT
APGQAYFINDGEPVNMFEFSRPVVEACGERYPRLRVPGRLVHFVMTVWQKLHFRFGLPKPLLEPLSVERIYLDNHFSIAK
AERDLGYRPLFTTEQAMQACLPYYVELYEKMKADGAKTPAVQAVRTTPPAG

Sequences:

>Translated_371_residues
MADATLTTELGRVLVTGGSGFVGANLVTELLERGHHVRSFDRAPSPLPPHPLLETLEGDICDPETVAAAVAGVDTVFHTA
AIIDLMGGASVTDEYRRRSFAVNVGGTENLVRAGRAAGVKRFVYTASNSVVMGGQPIVAGDETLPYTDRFNDLYTETKVA
AEKYVLAQNGIDGMLTCSIRPSGIWGRGDQTMFRKVFESVLAGHVKVLVGSKNVKLDNSYVHNLIHGFILAAEHLVPGGT
APGQAYFINDGEPVNMFEFSRPVVEACGERYPRLRVPGRLVHFVMTVWQKLHFRFGLPKPLLEPLSVERIYLDNHFSIAK
AERDLGYRPLFTTEQAMQACLPYYVELYEKMKADGAKTPAVQAVRTTPPAG
>Mature_370_residues
ADATLTTELGRVLVTGGSGFVGANLVTELLERGHHVRSFDRAPSPLPPHPLLETLEGDICDPETVAAAVAGVDTVFHTAA
IIDLMGGASVTDEYRRRSFAVNVGGTENLVRAGRAAGVKRFVYTASNSVVMGGQPIVAGDETLPYTDRFNDLYTETKVAA
EKYVLAQNGIDGMLTCSIRPSGIWGRGDQTMFRKVFESVLAGHVKVLVGSKNVKLDNSYVHNLIHGFILAAEHLVPGGTA
PGQAYFINDGEPVNMFEFSRPVVEACGERYPRLRVPGRLVHFVMTVWQKLHFRFGLPKPLLEPLSVERIYLDNHFSIAKA
ERDLGYRPLFTTEQAMQACLPYYVELYEKMKADGAKTPAVQAVRTTPPAG

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI310132178, Length=338, Percent_Identity=35.207100591716, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI310113012, Length=338, Percent_Identity=35.207100591716, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI239745448, Length=338, Percent_Identity=35.207100591716, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI116268111, Length=333, Percent_Identity=36.9369369369369, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI193211614, Length=345, Percent_Identity=30.7246376811594, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI8393516, Length=345, Percent_Identity=30.7246376811594, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI260763931, Length=365, Percent_Identity=30.958904109589, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4504509, Length=365, Percent_Identity=30.958904109589, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4504507, Length=372, Percent_Identity=30.1075268817204, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI19923621, Length=359, Percent_Identity=33.7047353760446, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI218563686, Length=201, Percent_Identity=36.81592039801, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI218563684, Length=201, Percent_Identity=36.81592039801, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI87081792, Length=334, Percent_Identity=23.0538922155689, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786974, Length=163, Percent_Identity=33.1288343558282, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17570557, Length=353, Percent_Identity=30.5949008498584, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17509709, Length=358, Percent_Identity=30.1675977653631, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI193211272, Length=334, Percent_Identity=27.2455089820359, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321437, Length=354, Percent_Identity=27.683615819209, Blast_Score=119, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 40475; Mature: 40344

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADATLTTELGRVLVTGGSGFVGANLVTELLERGHHVRSFDRAPSPLPPHPLLETLEGDI
CCCCHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHCCCC
CDPETVAAAVAGVDTVFHTAAIIDLMGGASVTDEYRRRSFAVNVGGTENLVRAGRAAGVK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEEECCCHHHHHHHHHHHCCE
RFVYTASNSVVMGGQPIVAGDETLPYTDRFNDLYTETKVAAEKYVLAQNGIDGMLTCSIR
EEEEECCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
PSGIWGRGDQTMFRKVFESVLAGHVKVLVGSKNVKLDNSYVHNLIHGFILAAEHLVPGGT
CCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHCCCCC
APGQAYFINDGEPVNMFEFSRPVVEACGERYPRLRVPGRLVHFVMTVWQKLHFRFGLPKP
CCCCEEEECCCCCCCHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
LLEPLSVERIYLDNHFSIAKAERDLGYRPLFTTEQAMQACLPYYVELYEKMKADGAKTPA
HHCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
VQAVRTTPPAG
CEEEECCCCCC
>Mature Secondary Structure 
ADATLTTELGRVLVTGGSGFVGANLVTELLERGHHVRSFDRAPSPLPPHPLLETLEGDI
CCCHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHCCCC
CDPETVAAAVAGVDTVFHTAAIIDLMGGASVTDEYRRRSFAVNVGGTENLVRAGRAAGVK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEEECCCHHHHHHHHHHHCCE
RFVYTASNSVVMGGQPIVAGDETLPYTDRFNDLYTETKVAAEKYVLAQNGIDGMLTCSIR
EEEEECCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
PSGIWGRGDQTMFRKVFESVLAGHVKVLVGSKNVKLDNSYVHNLIHGFILAAEHLVPGGT
CCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHCCCCC
APGQAYFINDGEPVNMFEFSRPVVEACGERYPRLRVPGRLVHFVMTVWQKLHFRFGLPKP
CCCCEEEECCCCCCCHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
LLEPLSVERIYLDNHFSIAKAERDLGYRPLFTTEQAMQACLPYYVELYEKMKADGAKTPA
HHCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
VQAVRTTPPAG
CEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]