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The map label for this gene is fadB [H]

Identifier: 119869763

GI number: 119869763

Start: 3950685

End: 3951401

Strand: Direct

Name: fadB [H]

Synonym: Mkms_3731

Alternate gene names: 119869763

Gene position: 3950685-3951401 (Clockwise)

Preceding gene: 119869756

Following gene: 119869764

Centisome position: 68.86

GC content: 70.99

Gene sequence:

>717_bases
ATGGTCGACCTCGAGATCCAGGACGGTCTGGCCGTCATCACGATCGATCGCCCGCATGCCCGCAACGCGATCTCGCTGGA
GACGATGGGGCAGCTGGAGAAGGCGCTCGACGGCGCGCAGGGAGCCAGGGCGCTGGTCGTGACCGGGGCCGGTGACCGCG
CGTTCGTCTCCGGCGGGGACCTCAAGGAACTCAGTGCGCTGCGCACCGAACCCGAGGCATCGGCGATGGCGCTGCGGATG
CGCACGATCTGCGACCGGATCGCCGAGTTCCCGGGTCCGGTCGTCGCGGCGCTGAACGGCCACGCACTGGGCGGCGGCGC
CGAGGTGGCGGTGGCGGCCGACATCCGCCTCGCGGCCGACGACATCCGCATCGGCTTCAACCAGGTCTCGCTGGAGATCA
TGCCGGCCTGGGGCGGCGCCGAACGGCTGGCCGGCCTGGTCGGCAAGAGTCGGGCGCTCCTGCTGGCGGGCACCGGCCGG
ATCCTGACCGCCGCGGAGGCCTACGAGCTGAATCTGGTGGACATGGTGTTGCCGCGCGAGGGATTCGCCGAAGGCTGGCG
CACGATCGCCCGTTCATTGGCCGGCCGACCGGCCGGCGAGATCAAACGGGTGATCGACGGCGTCCAAGCCACCGAGGCCG
TCGCGGCGTTCGCACGGCTGTGGGTGGCCGACGAACACTGGGCGGCCGCTGACAGGGTGATGAAACGCGCCAAGTGA

Upstream 100 bases:

>100_bases
CGGCAAGCGGTGCGCCGCCGCAGTTGCTCGCGCCGGCGCCCTCTCGGTGATGCTATTCTCCGCAGCCGAGAATGCCAATA
CCGAACTGGGAGAACGTCTG

Downstream 100 bases:

>100_bases
TCCGACCGCCCGGAGGTGCCGGTTCTCTCTAGACGAGAATGGCATTTCCCACTAGGGTCACCGGTGTGCAGGTTCGCGGG
GTTGCGGCCTGCCCGGACAC

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MVDLEIQDGLAVITIDRPHARNAISLETMGQLEKALDGAQGARALVVTGAGDRAFVSGGDLKELSALRTEPEASAMALRM
RTICDRIAEFPGPVVAALNGHALGGGAEVAVAADIRLAADDIRIGFNQVSLEIMPAWGGAERLAGLVGKSRALLLAGTGR
ILTAAEAYELNLVDMVLPREGFAEGWRTIARSLAGRPAGEIKRVIDGVQATEAVAAFARLWVADEHWAAADRVMKRAK

Sequences:

>Translated_238_residues
MVDLEIQDGLAVITIDRPHARNAISLETMGQLEKALDGAQGARALVVTGAGDRAFVSGGDLKELSALRTEPEASAMALRM
RTICDRIAEFPGPVVAALNGHALGGGAEVAVAADIRLAADDIRIGFNQVSLEIMPAWGGAERLAGLVGKSRALLLAGTGR
ILTAAEAYELNLVDMVLPREGFAEGWRTIARSLAGRPAGEIKRVIDGVQATEAVAAFARLWVADEHWAAADRVMKRAK
>Mature_238_residues
MVDLEIQDGLAVITIDRPHARNAISLETMGQLEKALDGAQGARALVVTGAGDRAFVSGGDLKELSALRTEPEASAMALRM
RTICDRIAEFPGPVVAALNGHALGGGAEVAVAADIRLAADDIRIGFNQVSLEIMPAWGGAERLAGLVGKSRALLLAGTGR
ILTAAEAYELNLVDMVLPREGFAEGWRTIARSLAGRPAGEIKRVIDGVQATEAVAAFARLWVADEHWAAADRVMKRAK

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=181, Percent_Identity=32.0441988950276, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI213417737, Length=180, Percent_Identity=33.8888888888889, Blast_Score=91, Evalue=6e-19,
Organism=Homo sapiens, GI157694516, Length=180, Percent_Identity=33.8888888888889, Blast_Score=91, Evalue=6e-19,
Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=29.1208791208791, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI68989263, Length=195, Percent_Identity=31.2820512820513, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI157694520, Length=152, Percent_Identity=33.5526315789474, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4502327, Length=176, Percent_Identity=33.5227272727273, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI70995211, Length=190, Percent_Identity=28.9473684210526, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI31542718, Length=198, Percent_Identity=28.7878787878788, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1790281, Length=192, Percent_Identity=32.8125, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788682, Length=208, Percent_Identity=33.6538461538462, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787659, Length=178, Percent_Identity=34.8314606741573, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788597, Length=175, Percent_Identity=32.5714285714286, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI221142681, Length=184, Percent_Identity=33.1521739130435, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787660, Length=216, Percent_Identity=30.5555555555556, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=173, Percent_Identity=31.7919075144509, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25141363, Length=228, Percent_Identity=29.8245614035088, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=175, Percent_Identity=35.4285714285714, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=174, Percent_Identity=35.0574712643678, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=180, Percent_Identity=31.1111111111111, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=180, Percent_Identity=29.4444444444444, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=180, Percent_Identity=29.4444444444444, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=180, Percent_Identity=29.4444444444444, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653139, Length=174, Percent_Identity=36.2068965517241, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI20129971, Length=174, Percent_Identity=35.0574712643678, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24653477, Length=174, Percent_Identity=35.0574712643678, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28571730, Length=158, Percent_Identity=30.379746835443, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571729, Length=158, Percent_Identity=30.379746835443, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21357171, Length=181, Percent_Identity=30.3867403314917, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921000, Length=179, Percent_Identity=31.8435754189944, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24583077, Length=179, Percent_Identity=31.8435754189944, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24583079, Length=179, Percent_Identity=31.8435754189944, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24583165, Length=189, Percent_Identity=28.042328042328, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19921018, Length=191, Percent_Identity=29.3193717277487, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24650670, Length=182, Percent_Identity=29.6703296703297, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 25069; Mature: 25069

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDLEIQDGLAVITIDRPHARNAISLETMGQLEKALDGAQGARALVVTGAGDRAFVSGGD
CEEEEECCCEEEEEECCCCCCCEECHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCC
LKELSALRTEPEASAMALRMRTICDRIAEFPGPVVAALNGHALGGGAEVAVAADIRLAAD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEEEECC
DIRIGFNQVSLEIMPAWGGAERLAGLVGKSRALLLAGTGRILTAAEAYELNLVDMVLPRE
HHEECEEEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCEEEEECHHEEEEEEEECCCC
GFAEGWRTIARSLAGRPAGEIKRVIDGVQATEAVAAFARLWVADEHWAAADRVMKRAK
HHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVDLEIQDGLAVITIDRPHARNAISLETMGQLEKALDGAQGARALVVTGAGDRAFVSGGD
CEEEEECCCEEEEEECCCCCCCEECHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCC
LKELSALRTEPEASAMALRMRTICDRIAEFPGPVVAALNGHALGGGAEVAVAADIRLAAD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEEEECC
DIRIGFNQVSLEIMPAWGGAERLAGLVGKSRALLLAGTGRILTAAEAYELNLVDMVLPRE
HHEECEEEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCEEEEECHHEEEEEEEECCCC
GFAEGWRTIARSLAGRPAGEIKRVIDGVQATEAVAAFARLWVADEHWAAADRVMKRAK
HHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]