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The map label for this gene is tgs1 [H]

Identifier: 119869504

GI number: 119869504

Start: 3647001

End: 3648416

Strand: Direct

Name: tgs1 [H]

Synonym: Mkms_3472

Alternate gene names: 119869504

Gene position: 3647001-3648416 (Clockwise)

Preceding gene: 119869502

Following gene: 119869505

Centisome position: 63.57

GC content: 68.43

Gene sequence:

>1416_bases
TTGCGCGGCCCACAGGGAGCGGACAGTGGTGACATGGACCACCTGAGCACCCTCGACGCGAGCTTCCTCGAGGCCGAGGA
CTCCGACCCCCACGTCAGCCTCGCCATCGGTTCGGTGTCCGTACTCGACGGTCCGGTTCCCGACGACGACGCGTTCGTAT
CGGGCCTCGCGGACCGAGTGCTCGCCGTGCCGCGGTTCCGGCAGGTGCTTCGCACGCATCCACTGGACCTCGAGGCACCG
GAGTGGGCCGACGCGGAGAACTTCGACATCTCCCATCACGTGCACCGGACCGCGCTGCCGCATCCGGGCGACGACGCGGC
GCTGTTCCGGCTGACCGCCGACGTGATGGAACGCCGACTCGACCGCGAACGGCCACTGTGGGAATGCTGGATCGTCGAGG
GTCTGCCGGACGACCAGTGGGCCATGATCATGAAACTGCACCACTGCATCGCCGACGGGATCGCGACGATGCACATGCTG
GCCGGGCTGAGTGACAGCGGCGAGGGCGATACGTACGCCACGGAGATCCGGGCGGCCGGCAGCACAGCGCAGCGCAGGTT
CCGGTTACCGCAACTGAGTCCCGATCCGCGGCAATGGGTCGGCACCCTGTGGCGGACCTCGACGGGTCTGGTCGGGGCCG
CCGCACGCGGGGTGGGCGGGGCCGCCGAGATCGTAGGCGGCCTCCTGCGGCCGAGCCCCACATCACTGGTCGGATCGGTG
AGCACGATGCGGCGTTTCGCGGGTGCCGAGGTGTCGCTCGACGACGCGCAGCACGTCTGTTCCGCCTTCGGGGTGACCCT
CAACGACGTGGTGCTCGCCGCGATCACCGACGCCTTCCGCGCCATGTTGATCCGCCGGGGCGAGAAACCCCAGGGCAATT
CGCTGCGCACGCTGGTACCGGTGTCGGTCCGCGCGAACAACGCCGTCGGCAAGGTCGACAACAGGGTGTCGCTGATGCTC
CCGTACCTCCCGGTCGACAAAGCCGATCCCGAAGAACAGCTGATGACCGTCCACGCGCGCTTGACCCAGGCCAAGAACAG
CGGGCAGCGGCAGGCCGGACACGCCGTCATCTCCGCGACGAACATCATCCCGTTCCCGTTGACCGCGTGGGCGGTTCGCG
CGATGACCCGGCTCCCGCAACGCAGCGTCGTCACCGTCGCGACGAACGTGCCCGGTCCCCGGCACCACATGCAGATCATG
GGGCGCAAGGTCGTGCGCATGTTCCCGATCCCGCCGATCGCGCTCGGCCTGCGCACCGGAATCGCGATCGTGACCTACGC
CGACCGGTTGGTCTTCGGGATCACCGGTGACTTCGACACCGCACCCGACGTCGACGACCTCGCCCGCGGTATCGAACACG
CGATGGACCGTCTCGTGGAGATCAGCACCGACGCCGAGAACCGACGACCTCACTAG

Upstream 100 bases:

>100_bases
ACGTTAGGGCTGCCGGGGAGCACCGGACCAGGGCCGTTGGTCTCTTATCCGATCAGGACGTCGATTCGTTCGGACGATCG
GTTCGGGACTTCAGACCCTG

Downstream 100 bases:

>100_bases
GGGAGCCCGAATCCATGAGTCAGGGCCGCGCGGACACCGACGTCCTCGTCAAGGCGATCCGGCTGGCGTGCAGAGCGCCG
TCACTGCACAACAGCCAGCC

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: TGS1; Probable triacylglycerol synthase tgs1 [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MRGPQGADSGDMDHLSTLDASFLEAEDSDPHVSLAIGSVSVLDGPVPDDDAFVSGLADRVLAVPRFRQVLRTHPLDLEAP
EWADAENFDISHHVHRTALPHPGDDAALFRLTADVMERRLDRERPLWECWIVEGLPDDQWAMIMKLHHCIADGIATMHML
AGLSDSGEGDTYATEIRAAGSTAQRRFRLPQLSPDPRQWVGTLWRTSTGLVGAAARGVGGAAEIVGGLLRPSPTSLVGSV
STMRRFAGAEVSLDDAQHVCSAFGVTLNDVVLAAITDAFRAMLIRRGEKPQGNSLRTLVPVSVRANNAVGKVDNRVSLML
PYLPVDKADPEEQLMTVHARLTQAKNSGQRQAGHAVISATNIIPFPLTAWAVRAMTRLPQRSVVTVATNVPGPRHHMQIM
GRKVVRMFPIPPIALGLRTGIAIVTYADRLVFGITGDFDTAPDVDDLARGIEHAMDRLVEISTDAENRRPH

Sequences:

>Translated_471_residues
MRGPQGADSGDMDHLSTLDASFLEAEDSDPHVSLAIGSVSVLDGPVPDDDAFVSGLADRVLAVPRFRQVLRTHPLDLEAP
EWADAENFDISHHVHRTALPHPGDDAALFRLTADVMERRLDRERPLWECWIVEGLPDDQWAMIMKLHHCIADGIATMHML
AGLSDSGEGDTYATEIRAAGSTAQRRFRLPQLSPDPRQWVGTLWRTSTGLVGAAARGVGGAAEIVGGLLRPSPTSLVGSV
STMRRFAGAEVSLDDAQHVCSAFGVTLNDVVLAAITDAFRAMLIRRGEKPQGNSLRTLVPVSVRANNAVGKVDNRVSLML
PYLPVDKADPEEQLMTVHARLTQAKNSGQRQAGHAVISATNIIPFPLTAWAVRAMTRLPQRSVVTVATNVPGPRHHMQIM
GRKVVRMFPIPPIALGLRTGIAIVTYADRLVFGITGDFDTAPDVDDLARGIEHAMDRLVEISTDAENRRPH
>Mature_471_residues
MRGPQGADSGDMDHLSTLDASFLEAEDSDPHVSLAIGSVSVLDGPVPDDDAFVSGLADRVLAVPRFRQVLRTHPLDLEAP
EWADAENFDISHHVHRTALPHPGDDAALFRLTADVMERRLDRERPLWECWIVEGLPDDQWAMIMKLHHCIADGIATMHML
AGLSDSGEGDTYATEIRAAGSTAQRRFRLPQLSPDPRQWVGTLWRTSTGLVGAAARGVGGAAEIVGGLLRPSPTSLVGSV
STMRRFAGAEVSLDDAQHVCSAFGVTLNDVVLAAITDAFRAMLIRRGEKPQGNSLRTLVPVSVRANNAVGKVDNRVSLML
PYLPVDKADPEEQLMTVHARLTQAKNSGQRQAGHAVISATNIIPFPLTAWAVRAMTRLPQRSVVTVATNVPGPRHHMQIM
GRKVVRMFPIPPIALGLRTGIAIVTYADRLVFGITGDFDTAPDVDDLARGIEHAMDRLVEISTDAENRRPH

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Prefers C(26:0)-CoA over C(18:1)-CoA. TG synthesis activity increases in M.tuberculosis upon oxygen depletion and NO

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 51118; Mature: 51118

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGPQGADSGDMDHLSTLDASFLEAEDSDPHVSLAIGSVSVLDGPVPDDDAFVSGLADRV
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECEEEEECCCCCCHHHHHHHHHHHH
LAVPRFRQVLRTHPLDLEAPEWADAENFDISHHVHRTALPHPGDDAALFRLTADVMERRL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
DRERPLWECWIVEGLPDDQWAMIMKLHHCIADGIATMHMLAGLSDSGEGDTYATEIRAAG
HHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
STAQRRFRLPQLSPDPRQWVGTLWRTSTGLVGAAARGVGGAAEIVGGLLRPSPTSLVGSV
CHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHCCCCCHHHHHHH
STMRRFAGAEVSLDDAQHVCSAFGVTLNDVVLAAITDAFRAMLIRRGEKPQGNSLRTLVP
HHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
VSVRANNAVGKVDNRVSLMLPYLPVDKADPEEQLMTVHARLTQAKNSGQRQAGHAVISAT
EEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCEEEEEC
NIIPFPLTAWAVRAMTRLPQRSVVTVATNVPGPRHHMQIMGRKVVRMFPIPPIALGLRTG
CCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
IAIVTYADRLVFGITGDFDTAPDVDDLARGIEHAMDRLVEISTDAENRRPH
EEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRGPQGADSGDMDHLSTLDASFLEAEDSDPHVSLAIGSVSVLDGPVPDDDAFVSGLADRV
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECEEEEECCCCCCHHHHHHHHHHHH
LAVPRFRQVLRTHPLDLEAPEWADAENFDISHHVHRTALPHPGDDAALFRLTADVMERRL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
DRERPLWECWIVEGLPDDQWAMIMKLHHCIADGIATMHMLAGLSDSGEGDTYATEIRAAG
HHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
STAQRRFRLPQLSPDPRQWVGTLWRTSTGLVGAAARGVGGAAEIVGGLLRPSPTSLVGSV
CHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHCCCCCHHHHHHH
STMRRFAGAEVSLDDAQHVCSAFGVTLNDVVLAAITDAFRAMLIRRGEKPQGNSLRTLVP
HHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
VSVRANNAVGKVDNRVSLMLPYLPVDKADPEEQLMTVHARLTQAKNSGQRQAGHAVISAT
EEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCEEEEEC
NIIPFPLTAWAVRAMTRLPQRSVVTVATNVPGPRHHMQIMGRKVVRMFPIPPIALGLRTG
CCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
IAIVTYADRLVFGITGDFDTAPDVDDLARGIEHAMDRLVEISTDAENRRPH
EEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]