Click here to switch to the map view.

The map label for this gene is ramA [H]

Identifier: 119869429

GI number: 119869429

Start: 3565586

End: 3566038

Strand: Direct

Name: ramA [H]

Synonym: Mkms_3397

Alternate gene names: 119869429

Gene position: 3565586-3566038 (Clockwise)

Preceding gene: 119869428

Following gene: 119869430

Centisome position: 62.15

GC content: 72.19

Gene sequence:

>453_bases
ATGCCGCAGGTGCCGCCGGCCCGCTATCTGCAGCGTGCCCGTGACCTCGTCGACGCCCGGTACGCCGAGCCGATCACCGT
CGACGACCTCGCCGCGGCCGCCGGTCTGTCGCGGGCGCACTTCAGCCGCATGTTCACCCGCACCTTCGGGGAGTCGCCGC
GCGAGTACCTGCAGACCCGCCGACTCGAACGCGCCGCCGCGCTGTTGCGTTACACCGACCGTTCGGTGGCCGACATCTGC
GCGATGGTCGGGCTGCAGAGCGTCGGGTCGTTCACCACCAGCTTCGCCCGGGTCTACGGTCTGCCGCCAGCCGCCTACCG
GGCCAGCCTGCCCCCGGCGGCCACGTACGCGCGGGTGCCGACATGCATCCTCAAGCGCGACACCCGTCCGCCCGCCGACA
GCCGGGTGAAGACAGCACACGGGGAGAAGACGAAGGCGCCCGTCCGGTCGTAG

Upstream 100 bases:

>100_bases
CAATCACCCTGCTCTGTGAAGTCCGGACTTTCCTCGAACCGTTTCCGGTCCGCGGCCGCCCGGCCAACTCGTCCGCGCAG
ATCACCGTACCCTCATATGT

Downstream 100 bases:

>100_bases
CGTTCATTCCATGAAGATCGCAAGTGCCCACCTGTGGGTTCATGACCAGCAAGCAGCGCTCGAATTCTGGACCTCGAAGG
TCGGAATGGAAGTGCGCCAG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MPQVPPARYLQRARDLVDARYAEPITVDDLAAAAGLSRAHFSRMFTRTFGESPREYLQTRRLERAAALLRYTDRSVADIC
AMVGLQSVGSFTTSFARVYGLPPAAYRASLPPAATYARVPTCILKRDTRPPADSRVKTAHGEKTKAPVRS

Sequences:

>Translated_150_residues
MPQVPPARYLQRARDLVDARYAEPITVDDLAAAAGLSRAHFSRMFTRTFGESPREYLQTRRLERAAALLRYTDRSVADIC
AMVGLQSVGSFTTSFARVYGLPPAAYRASLPPAATYARVPTCILKRDTRPPADSRVKTAHGEKTKAPVRS
>Mature_149_residues
PQVPPARYLQRARDLVDARYAEPITVDDLAAAAGLSRAHFSRMFTRTFGESPREYLQTRRLERAAALLRYTDRSVADICA
MVGLQSVGSFTTSFARVYGLPPAAYRASLPPAATYARVPTCILKRDTRPPADSRVKTAHGEKTKAPVRS

Specific function: Probable transcriptional activator [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=120, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 16569; Mature: 16438

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQVPPARYLQRARDLVDARYAEPITVDDLAAAAGLSRAHFSRMFTRTFGESPREYLQTR
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RLERAAALLRYTDRSVADICAMVGLQSVGSFTTSFARVYGLPPAAYRASLPPAATYARVP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHH
TCILKRDTRPPADSRVKTAHGEKTKAPVRS
HHHHHCCCCCCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PQVPPARYLQRARDLVDARYAEPITVDDLAAAAGLSRAHFSRMFTRTFGESPREYLQTR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RLERAAALLRYTDRSVADICAMVGLQSVGSFTTSFARVYGLPPAAYRASLPPAATYARVP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHH
TCILKRDTRPPADSRVKTAHGEKTKAPVRS
HHHHHCCCCCCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2193928; 7551053 [H]