Click here to switch to the map view.

The map label for this gene is fadD25 [H]

Identifier: 119869196

GI number: 119869196

Start: 3312694

End: 3314499

Strand: Direct

Name: fadD25 [H]

Synonym: Mkms_3164

Alternate gene names: 119869196

Gene position: 3312694-3314499 (Clockwise)

Preceding gene: 119869195

Following gene: 119869197

Centisome position: 57.74

GC content: 67.88

Gene sequence:

>1806_bases
ATGCGCGTTTTGGGCATCGGTTGCCTTATGCTCGGGAGGATTGTTCGCTACCGCAAGGACGAAGGGCCCGCCATGACTGC
GTCAACACTTCCCACCGTGCTGCGGGAACGCGCAAGTCTGCAGCCCAACGACACGGCATTCACGTTCATCGACTACGAGA
AGGCCGTCGAAGGCGTCCACGAGTCGCTCACGTGGGCGCAACTGTACCGCCGCACGGTGAACCTCGCGCACGAGCTGAGC
GAACACGCGCAGCCCGGCGACCGGGCCGTGATCGTCGCACCGCAGGGGCTCGACTACCTGGTCGCGTTCCTGGGCTCCCT
GCAGGCCGGACTCATCGCGGTGCCGCTGTCGACACCCATGCCGGGGGTGCACGACGAGCGAATCAGCGCGGTGCTGCGCG
ACGCCGCCCCCACCGTGGTGCTGACCACCTCGTCCATCGGCGCCATGGTCACCGAATACGTGGCGCCCGACGACGCCGAG
ACCGCGGCGACGCTGATCGAGGTCGACCTGCTGGACCTCGAGAGCAGACGGAAATCGGCGGCCCGCCGGGTGGAGCGGCC
CGAGACGGCCTATCTGCAGTACACGTCGGGCTCGACGCGCACGCCGGCGGGCGTGATGGTGTCCTACCGCAATCTGTCGG
CGAACTTCGAACAGATCATGGCGTCATTCCTGGCGCATTACGGCGGCATCGCACCGCCCGGGACCACCGCGGTCACGTGG
CTGCCCTTCTACCACGACATGGGGCTGCTGCTCGGGGTGTTCGCGCCGATCCTCGCGGGCTGGCCGACCGTGTTCACCAC
ACCGCTGTCCTTCCTGGCCCGGCCGGCCAGGTGGATGCAGATGGTGGCCGGCAGCCCGCTTGCCATCACCGCGGGACCGA
ACTTCGCCTTCGAACTCGCCGTCGGCAAGACCTCCGACGACGACATGGCCGGGTACGACCTCGGCAACACCTGCATCATC
ATCAGCGGGAGCGAGCGGGTGCACGACGCGACCCTGAAGCGTTTCGCGCAGCGCTTCCGCAAGTTCAACCTGCCCGAAGA
GGTCCTGCGCCCGGCGTACGGGCTGGCCGAGGCGACGCTCTACGTCGCGACGAATTCGCCCGGCAAACCCCCGACCATCG
TGCATTTCGAACCGGAGAAACTGTCGGCCGGTCACGCCAAACGTACCGAGACCGGGACCCCGCTGGTCAGCTACGGCAGC
CCCGACTCCCCCGCGGTCCGGATCGTCGATCCGCAGACTCGCCGCGAGTTGTCCGCCGGGGCGATCGGCGAGATCTGGGT
GCGTGGCGAGAACGTGTGCCTCGGCTACTGGGGCAAACCCGAACAGACCGAGGAAGTGTTCAACGCGTTCCTCGACTCCC
CGTCGCCGGGTACCGACGCGGGTCCGTGGCTGCGCACCGGCGATCTGGGGTTCATCTCCGACGACGAGCTGTTCATCATG
GGCCGGCTCAAGGATCTGCTGATCGTGCGCGGCCGCAACCACTATCCGGACGACATCGAGGGCACGATCCAGGAGATCTC
CGGCGGCAGGGTCGCGGCGATCTCGGTCGAACAGGACCGGACCGAACAGCTGGTCGCCATCGTGGAGGTCAAGAAGCGCG
GGGACACCGACGAGGACGTGGCCGCCTACTTCGCGGACCTGAAGAACCGGGTGACCAGCGCGATCTCCACCGCGCACGGG
ATCAGCGCAGCCGACCTGGTGCTGGTGCCGCGCGGCTCGATCCCGATCACCACGAGCGGCAAGGTGCGTCGCGCCTCCTG
CGCGGAGCTCTACCGCGACGGCGCGTTCACCCGGCTGGACGCCTGA

Upstream 100 bases:

>100_bases
GGCGACGACAGCAATCAGATCCGGGCATTTCGGTAGGTAGACAGGGCAAGCCGGACGGTTCATCCGCACCACTCCCAGCG
CATTTCACCGTCATCGGCGA

Downstream 100 bases:

>100_bases
CAGCACGCGGGCCAGCTCACCATGAGCGGCTTTCGCCGAACACGCGGCCGGCCCCTGAGCGCCGCCGACTGTATGAACGG
ACTCCTTCGATGACACCACA

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MRVLGIGCLMLGRIVRYRKDEGPAMTASTLPTVLRERASLQPNDTAFTFIDYEKAVEGVHESLTWAQLYRRTVNLAHELS
EHAQPGDRAVIVAPQGLDYLVAFLGSLQAGLIAVPLSTPMPGVHDERISAVLRDAAPTVVLTTSSIGAMVTEYVAPDDAE
TAATLIEVDLLDLESRRKSAARRVERPETAYLQYTSGSTRTPAGVMVSYRNLSANFEQIMASFLAHYGGIAPPGTTAVTW
LPFYHDMGLLLGVFAPILAGWPTVFTTPLSFLARPARWMQMVAGSPLAITAGPNFAFELAVGKTSDDDMAGYDLGNTCII
ISGSERVHDATLKRFAQRFRKFNLPEEVLRPAYGLAEATLYVATNSPGKPPTIVHFEPEKLSAGHAKRTETGTPLVSYGS
PDSPAVRIVDPQTRRELSAGAIGEIWVRGENVCLGYWGKPEQTEEVFNAFLDSPSPGTDAGPWLRTGDLGFISDDELFIM
GRLKDLLIVRGRNHYPDDIEGTIQEISGGRVAAISVEQDRTEQLVAIVEVKKRGDTDEDVAAYFADLKNRVTSAISTAHG
ISAADLVLVPRGSIPITTSGKVRRASCAELYRDGAFTRLDA

Sequences:

>Translated_601_residues
MRVLGIGCLMLGRIVRYRKDEGPAMTASTLPTVLRERASLQPNDTAFTFIDYEKAVEGVHESLTWAQLYRRTVNLAHELS
EHAQPGDRAVIVAPQGLDYLVAFLGSLQAGLIAVPLSTPMPGVHDERISAVLRDAAPTVVLTTSSIGAMVTEYVAPDDAE
TAATLIEVDLLDLESRRKSAARRVERPETAYLQYTSGSTRTPAGVMVSYRNLSANFEQIMASFLAHYGGIAPPGTTAVTW
LPFYHDMGLLLGVFAPILAGWPTVFTTPLSFLARPARWMQMVAGSPLAITAGPNFAFELAVGKTSDDDMAGYDLGNTCII
ISGSERVHDATLKRFAQRFRKFNLPEEVLRPAYGLAEATLYVATNSPGKPPTIVHFEPEKLSAGHAKRTETGTPLVSYGS
PDSPAVRIVDPQTRRELSAGAIGEIWVRGENVCLGYWGKPEQTEEVFNAFLDSPSPGTDAGPWLRTGDLGFISDDELFIM
GRLKDLLIVRGRNHYPDDIEGTIQEISGGRVAAISVEQDRTEQLVAIVEVKKRGDTDEDVAAYFADLKNRVTSAISTAHG
ISAADLVLVPRGSIPITTSGKVRRASCAELYRDGAFTRLDA
>Mature_601_residues
MRVLGIGCLMLGRIVRYRKDEGPAMTASTLPTVLRERASLQPNDTAFTFIDYEKAVEGVHESLTWAQLYRRTVNLAHELS
EHAQPGDRAVIVAPQGLDYLVAFLGSLQAGLIAVPLSTPMPGVHDERISAVLRDAAPTVVLTTSSIGAMVTEYVAPDDAE
TAATLIEVDLLDLESRRKSAARRVERPETAYLQYTSGSTRTPAGVMVSYRNLSANFEQIMASFLAHYGGIAPPGTTAVTW
LPFYHDMGLLLGVFAPILAGWPTVFTTPLSFLARPARWMQMVAGSPLAITAGPNFAFELAVGKTSDDDMAGYDLGNTCII
ISGSERVHDATLKRFAQRFRKFNLPEEVLRPAYGLAEATLYVATNSPGKPPTIVHFEPEKLSAGHAKRTETGTPLVSYGS
PDSPAVRIVDPQTRRELSAGAIGEIWVRGENVCLGYWGKPEQTEEVFNAFLDSPSPGTDAGPWLRTGDLGFISDDELFIM
GRLKDLLIVRGRNHYPDDIEGTIQEISGGRVAAISVEQDRTEQLVAIVEVKKRGDTDEDVAAYFADLKNRVTSAISTAHG
ISAADLVLVPRGSIPITTSGKVRRASCAELYRDGAFTRLDA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=617, Percent_Identity=25.9319286871961, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI225735629, Length=617, Percent_Identity=25.6077795786062, Blast_Score=130, Evalue=5e-30,
Organism=Homo sapiens, GI45827692, Length=617, Percent_Identity=25.6077795786062, Blast_Score=130, Evalue=5e-30,
Organism=Homo sapiens, GI55749758, Length=541, Percent_Identity=24.9537892791128, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI225735625, Length=614, Percent_Identity=25.0814332247557, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI156151445, Length=572, Percent_Identity=23.9510489510489, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI45827694, Length=557, Percent_Identity=25.4937163375224, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1788107, Length=578, Percent_Identity=26.643598615917, Blast_Score=123, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI193202679, Length=583, Percent_Identity=24.0137221269297, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32563687, Length=531, Percent_Identity=24.2937853107345, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559526, Length=318, Percent_Identity=26.1006289308176, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24654656, Length=516, Percent_Identity=22.6744186046512, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI18859661, Length=562, Percent_Identity=25.0889679715303, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 65179; Mature: 65179

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLGIGCLMLGRIVRYRKDEGPAMTASTLPTVLRERASLQPNDTAFTFIDYEKAVEGVH
CEEEEHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHH
ESLTWAQLYRRTVNLAHELSEHAQPGDRAVIVAPQGLDYLVAFLGSLQAGLIAVPLSTPM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCCCC
PGVHDERISAVLRDAAPTVVLTTSSIGAMVTEYVAPDDAETAATLIEVDLLDLESRRKSA
CCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHEEEEEHHHHHHHHHHH
ARRVERPETAYLQYTSGSTRTPAGVMVSYRNLSANFEQIMASFLAHYGGIAPPGTTAVTW
HHHCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEE
LPFYHDMGLLLGVFAPILAGWPTVFTTPLSFLARPARWMQMVAGSPLAITAGPNFAFELA
EHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCEEEEECCCEEEEEE
VGKTSDDDMAGYDLGNTCIIISGSERVHDATLKRFAQRFRKFNLPEEVLRPAYGLAEATL
ECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEE
YVATNSPGKPPTIVHFEPEKLSAGHAKRTETGTPLVSYGSPDSPAVRIVDPQTRRELSAG
EEEECCCCCCCEEEEECCHHCCCCCCCCCCCCCCEEECCCCCCCEEEEECCHHHHHHCCC
AIGEIWVRGENVCLGYWGKPEQTEEVFNAFLDSPSPGTDAGPWLRTGDLGFISDDELFIM
CCEEEEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCEECCCCEEEE
GRLKDLLIVRGRNHYPDDIEGTIQEISGGRVAAISVEQDRTEQLVAIVEVKKRGDTDEDV
ECCCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHH
AAYFADLKNRVTSAISTAHGISAADLVLVPRGSIPITTSGKVRRASCAELYRDGAFTRLD
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCCCCHHHHHHHHHCCCCEECC
A
C
>Mature Secondary Structure
MRVLGIGCLMLGRIVRYRKDEGPAMTASTLPTVLRERASLQPNDTAFTFIDYEKAVEGVH
CEEEEHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHH
ESLTWAQLYRRTVNLAHELSEHAQPGDRAVIVAPQGLDYLVAFLGSLQAGLIAVPLSTPM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCCCC
PGVHDERISAVLRDAAPTVVLTTSSIGAMVTEYVAPDDAETAATLIEVDLLDLESRRKSA
CCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHEEEEEHHHHHHHHHHH
ARRVERPETAYLQYTSGSTRTPAGVMVSYRNLSANFEQIMASFLAHYGGIAPPGTTAVTW
HHHCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEE
LPFYHDMGLLLGVFAPILAGWPTVFTTPLSFLARPARWMQMVAGSPLAITAGPNFAFELA
EHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCEEEEECCCEEEEEE
VGKTSDDDMAGYDLGNTCIIISGSERVHDATLKRFAQRFRKFNLPEEVLRPAYGLAEATL
ECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEE
YVATNSPGKPPTIVHFEPEKLSAGHAKRTETGTPLVSYGSPDSPAVRIVDPQTRRELSAG
EEEECCCCCCCEEEEECCHHCCCCCCCCCCCCCCEEECCCCCCCEEEEECCHHHHHHCCC
AIGEIWVRGENVCLGYWGKPEQTEEVFNAFLDSPSPGTDAGPWLRTGDLGFISDDELFIM
CCEEEEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCEECCCCEEEE
GRLKDLLIVRGRNHYPDDIEGTIQEISGGRVAAISVEQDRTEQLVAIVEVKKRGDTDEDV
ECCCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHH
AAYFADLKNRVTSAISTAHGISAADLVLVPRGSIPITTSGKVRRASCAELYRDGAFTRLD
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCCCCHHHHHHHHHCCCCEECC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]