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The map label for this gene is fadD26 [H]

Identifier: 119868912

GI number: 119868912

Start: 3017604

End: 3019499

Strand: Reverse

Name: fadD26 [H]

Synonym: Mkms_2880

Alternate gene names: 119868912

Gene position: 3019499-3017604 (Counterclockwise)

Preceding gene: 119868916

Following gene: 119868911

Centisome position: 52.63

GC content: 67.51

Gene sequence:

>1896_bases
ATGACATTGACGGTCTATCCGACCGACGGCTCCGGCACGCTACGCGCGGATCTGGGGAGGACGCGCCGCGACGCCGGGCC
GCTTGCAGCGTCGTCGCGTGACGAGCACATACGGACTTCTCAACGGACGGCCACTGAAGAAACGAGTAGGACCATGACTG
AATCGTCTGTGACAGCCCTGCTGCAGGAGCGGGCGCTGCACCAGCCCGACGAGCCCGCGTACACGTTCATCGACTACGAC
GTCGACCCGAAGGGGTACGCCGAGACCCTCACCTGGGCTCAGCTGCACAACCGGGCACAGGTGCTGGCCGAGGAGCTGAT
GCTGTTCGCCGGCGCCGGTGACCGCGCCGCGATCCTCGCTCCCCAGGGCCTGGACTACATCGTCGCCTTCTTCGGCGCGC
TGCAGGCGGGACTCATCGCCGCTCCACTCTCGGTCCCGGCGTACGGCGTGCACGACGAGCGGATCTCGAGCGTGCTGCGG
GACTGCGCACCGTCGGTCATCCTGACGACATCGAGCGTCGCGGGTGAAGTCGGCCGGTATGCACTGGGCGGAGGCGGCCG
AGCGGCAACGGTGGTCGAGGTCGACTCGCTGGATCTCGACGCCCCCGCACAGTCTGCTGCGCCCCCGGCCCAGAAGTCCA
AGATCGCGTACTTGCAGTACACCTCGGGGTCGACGCGGGTGCCGGCCGGTGTGGTCGTCACCCACGACAACGTGGTGGTC
AACGTCGACCAGGCGTTCACCGACTACTTCGGTCACTCGGGGGCGACGCCTCCGGGCATGACCTTCGTATCGTGGCTGCC
CTTCTACCACGACATGGGTCTGATCAAGGGTGTGTGCGCGCCGTTGGTGAGCGGACGTTCCGCGGTCCTGATGAGCCCCA
TGGCATTCCTGCAGCGCCCCGCCCGCTGGATCCAGCAGATCGCGATCAATCCCGGATCATTCACGGCGGCACCGAATTTC
GCGTTCGAGTTGGCGGTCCGGCGGACCACCGACGAGGACATGGCCGGGCTCGATCTCGGCGCGGTGCTCGGAATCTGCAG
CGGAAGTGAACGGGTCCACGCGGCGACGGTCAACCGTTTCGTCGAACGCTTCAGCCGCTTCGGCCTGAATGCGGCGTCGG
TCAAACCCTCGTACGGGCTTGCCGAGGCCACCGTCTATGTGGCGACGTCCGACCGAGGCCACGCACCGAACATCGTCCGG
TTCGACTACGAGAAGCTGTCGAGCGGGCACGCGATGCGCTGTGAGAGCGAAGCCGCCGGTGGCACGGATCTGCTCGGTCA
CGGGACGGCCCGGTCGACCACACTGCGCATCGTCGATCCGGAGAGCGGCACCGAGAACCCCGCCGGCCAGGTCGGGGAGA
TCTGGGTCCATGGGGCGAACGTCGCCCGGGGTTACTGGCAGCGCCCGAAGGAGACCGAGCGGGTGTTCTCAGGGCGCCTG
GTCAAGCCGTCGGCGGGGACCGCATCGGGACCGTGGCTGCGCACCGGCGACCTCGGCGTCATCTCCGACGGTGAACTGTT
CATCATCGGCCGGATCAAGGACCTCCTGATCGTGGACGGGTCGAACCACTACCCGGACGACATCGAGGCCACGATCCAGG
AACTCTCCGGCGGTCGGGTGGCCGCGATCTCGGTGCCCAGCGGGGAGACCGAACATCTCGTCACCATCGTCGAGGTCAAG
AAGCGCGGCTCCGACGACGAGCACCGCGAACGGCTGCGCACCCTCAAACGCCGGATCACCGCGGCTGTTTCGACCACCCA
CCGGGTTCGGGCCGCCGACGTCGTGTTCGTCGCGCCCGGCGCCATCCCGATCACCACGAGCGGCAAGATCCGGCGTTCCA
CCTGTGTGGAGCGCTACGGGCGCGGGGAGTTCGAGCGTCTGGATCAGTCGGCATGA

Upstream 100 bases:

>100_bases
TCCGGTTTAAGTTTCGGAACACCGTTGTACGGTATGGCGATCAACGAAACCGCGAGGTGGTTCAAGATGACCGGAATTCG
TTGGTGGGGAGCACTGGGGG

Downstream 100 bases:

>100_bases
CACCGGCCACGCTCGACGACGCGAGGCTGCGCGACTGGTTGGTCACCTATCTGACCACGCACGTCGAGTGCTCACCCGAG
AGCATCGACTTCGACGCGTC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MTLTVYPTDGSGTLRADLGRTRRDAGPLAASSRDEHIRTSQRTATEETSRTMTESSVTALLQERALHQPDEPAYTFIDYD
VDPKGYAETLTWAQLHNRAQVLAEELMLFAGAGDRAAILAPQGLDYIVAFFGALQAGLIAAPLSVPAYGVHDERISSVLR
DCAPSVILTTSSVAGEVGRYALGGGGRAATVVEVDSLDLDAPAQSAAPPAQKSKIAYLQYTSGSTRVPAGVVVTHDNVVV
NVDQAFTDYFGHSGATPPGMTFVSWLPFYHDMGLIKGVCAPLVSGRSAVLMSPMAFLQRPARWIQQIAINPGSFTAAPNF
AFELAVRRTTDEDMAGLDLGAVLGICSGSERVHAATVNRFVERFSRFGLNAASVKPSYGLAEATVYVATSDRGHAPNIVR
FDYEKLSSGHAMRCESEAAGGTDLLGHGTARSTTLRIVDPESGTENPAGQVGEIWVHGANVARGYWQRPKETERVFSGRL
VKPSAGTASGPWLRTGDLGVISDGELFIIGRIKDLLIVDGSNHYPDDIEATIQELSGGRVAAISVPSGETEHLVTIVEVK
KRGSDDEHRERLRTLKRRITAAVSTTHRVRAADVVFVAPGAIPITTSGKIRRSTCVERYGRGEFERLDQSA

Sequences:

>Translated_631_residues
MTLTVYPTDGSGTLRADLGRTRRDAGPLAASSRDEHIRTSQRTATEETSRTMTESSVTALLQERALHQPDEPAYTFIDYD
VDPKGYAETLTWAQLHNRAQVLAEELMLFAGAGDRAAILAPQGLDYIVAFFGALQAGLIAAPLSVPAYGVHDERISSVLR
DCAPSVILTTSSVAGEVGRYALGGGGRAATVVEVDSLDLDAPAQSAAPPAQKSKIAYLQYTSGSTRVPAGVVVTHDNVVV
NVDQAFTDYFGHSGATPPGMTFVSWLPFYHDMGLIKGVCAPLVSGRSAVLMSPMAFLQRPARWIQQIAINPGSFTAAPNF
AFELAVRRTTDEDMAGLDLGAVLGICSGSERVHAATVNRFVERFSRFGLNAASVKPSYGLAEATVYVATSDRGHAPNIVR
FDYEKLSSGHAMRCESEAAGGTDLLGHGTARSTTLRIVDPESGTENPAGQVGEIWVHGANVARGYWQRPKETERVFSGRL
VKPSAGTASGPWLRTGDLGVISDGELFIIGRIKDLLIVDGSNHYPDDIEATIQELSGGRVAAISVPSGETEHLVTIVEVK
KRGSDDEHRERLRTLKRRITAAVSTTHRVRAADVVFVAPGAIPITTSGKIRRSTCVERYGRGEFERLDQSA
>Mature_630_residues
TLTVYPTDGSGTLRADLGRTRRDAGPLAASSRDEHIRTSQRTATEETSRTMTESSVTALLQERALHQPDEPAYTFIDYDV
DPKGYAETLTWAQLHNRAQVLAEELMLFAGAGDRAAILAPQGLDYIVAFFGALQAGLIAAPLSVPAYGVHDERISSVLRD
CAPSVILTTSSVAGEVGRYALGGGGRAATVVEVDSLDLDAPAQSAAPPAQKSKIAYLQYTSGSTRVPAGVVVTHDNVVVN
VDQAFTDYFGHSGATPPGMTFVSWLPFYHDMGLIKGVCAPLVSGRSAVLMSPMAFLQRPARWIQQIAINPGSFTAAPNFA
FELAVRRTTDEDMAGLDLGAVLGICSGSERVHAATVNRFVERFSRFGLNAASVKPSYGLAEATVYVATSDRGHAPNIVRF
DYEKLSSGHAMRCESEAAGGTDLLGHGTARSTTLRIVDPESGTENPAGQVGEIWVHGANVARGYWQRPKETERVFSGRLV
KPSAGTASGPWLRTGDLGVISDGELFIIGRIKDLLIVDGSNHYPDDIEATIQELSGGRVAAISVPSGETEHLVTIVEVKK
RGSDDEHRERLRTLKRRITAAVSTTHRVRAADVVFVAPGAIPITTSGKIRRSTCVERYGRGEFERLDQSA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=590, Percent_Identity=25.4237288135593, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI225735629, Length=617, Percent_Identity=23.9870340356564, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI45827692, Length=617, Percent_Identity=23.9870340356564, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI225735625, Length=617, Percent_Identity=23.9870340356564, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI55749758, Length=575, Percent_Identity=25.2173913043478, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI45827694, Length=559, Percent_Identity=24.5080500894454, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI45827696, Length=517, Percent_Identity=24.9516441005803, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI225735627, Length=517, Percent_Identity=24.9516441005803, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI45827698, Length=486, Percent_Identity=24.4855967078189, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1788107, Length=597, Percent_Identity=25.963149078727, Blast_Score=138, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI193202679, Length=604, Percent_Identity=23.1788079470199, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17559526, Length=563, Percent_Identity=24.1563055062167, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=409, Percent_Identity=22.2493887530562, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24654656, Length=621, Percent_Identity=23.6714975845411, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24581924, Length=556, Percent_Identity=25, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281365686, Length=155, Percent_Identity=30.9677419354839, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24653035, Length=184, Percent_Identity=30.9782608695652, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI161076582, Length=175, Percent_Identity=30.2857142857143, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 67590; Mature: 67459

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTVYPTDGSGTLRADLGRTRRDAGPLAASSRDEHIRTSQRTATEETSRTMTESSVTAL
CEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LQERALHQPDEPAYTFIDYDVDPKGYAETLTWAQLHNRAQVLAEELMLFAGAGDRAAILA
HHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEC
PQGLDYIVAFFGALQAGLIAAPLSVPAYGVHDERISSVLRDCAPSVILTTSSVAGEVGRY
CCCHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHCCE
ALGGGGRAATVVEVDSLDLDAPAQSAAPPAQKSKIAYLQYTSGSTRVPAGVVVTHDNVVV
EECCCCCEEEEEEEECCCCCCCCCCCCCCCHHCEEEEEEECCCCCCCCCEEEEECCCEEE
NVDQAFTDYFGHSGATPPGMTFVSWLPFYHDMGLIKGVCAPLVSGRSAVLMSPMAFLQRP
EEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHH
ARWIQQIAINPGSFTAAPNFAFELAVRRTTDEDMAGLDLGAVLGICSGSERVHAATVNRF
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCHHCCCCHHHHHHHCCCCCCCHHHHHHHH
VERFSRFGLNAASVKPSYGLAEATVYVATSDRGHAPNIVRFDYEKLSSGHAMRCESEAAG
HHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEECHHHHCCCCCEEECCCCCC
GTDLLGHGTARSTTLRIVDPESGTENPAGQVGEIWVHGANVARGYWQRPKETERVFSGRL
CCCEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHCCEE
VKPSAGTASGPWLRTGDLGVISDGELFIIGRIKDLLIVDGSNHYPDDIEATIQELSGGRV
ECCCCCCCCCCEEECCCEEEECCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHCCCCEE
AAISVPSGETEHLVTIVEVKKRGSDDEHRERLRTLKRRITAAVSTTHRVRAADVVFVAPG
EEEECCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCC
AIPITTSGKIRRSTCVERYGRGEFERLDQSA
CEEECCCCCCHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure 
TLTVYPTDGSGTLRADLGRTRRDAGPLAASSRDEHIRTSQRTATEETSRTMTESSVTAL
EEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LQERALHQPDEPAYTFIDYDVDPKGYAETLTWAQLHNRAQVLAEELMLFAGAGDRAAILA
HHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEC
PQGLDYIVAFFGALQAGLIAAPLSVPAYGVHDERISSVLRDCAPSVILTTSSVAGEVGRY
CCCHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHCCE
ALGGGGRAATVVEVDSLDLDAPAQSAAPPAQKSKIAYLQYTSGSTRVPAGVVVTHDNVVV
EECCCCCEEEEEEEECCCCCCCCCCCCCCCHHCEEEEEEECCCCCCCCCEEEEECCCEEE
NVDQAFTDYFGHSGATPPGMTFVSWLPFYHDMGLIKGVCAPLVSGRSAVLMSPMAFLQRP
EEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHH
ARWIQQIAINPGSFTAAPNFAFELAVRRTTDEDMAGLDLGAVLGICSGSERVHAATVNRF
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCHHCCCCHHHHHHHCCCCCCCHHHHHHHH
VERFSRFGLNAASVKPSYGLAEATVYVATSDRGHAPNIVRFDYEKLSSGHAMRCESEAAG
HHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEECHHHHCCCCCEEECCCCCC
GTDLLGHGTARSTTLRIVDPESGTENPAGQVGEIWVHGANVARGYWQRPKETERVFSGRL
CCCEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHCCEE
VKPSAGTASGPWLRTGDLGVISDGELFIIGRIKDLLIVDGSNHYPDDIEATIQELSGGRV
ECCCCCCCCCCEEECCCEEEECCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHCCCCEE
AAISVPSGETEHLVTIVEVKKRGSDDEHRERLRTLKRRITAAVSTTHRVRAADVVFVAPG
EEEECCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCC
AIPITTSGKIRRSTCVERYGRGEFERLDQSA
CEEECCCCCCHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]