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The map label for this gene is gatA [H]

Identifier: 119868840

GI number: 119868840

Start: 2927483

End: 2928886

Strand: Direct

Name: gatA [H]

Synonym: Mkms_2808

Alternate gene names: 119868840

Gene position: 2927483-2928886 (Clockwise)

Preceding gene: 119868838

Following gene: 119868841

Centisome position: 51.03

GC content: 70.3

Gene sequence:

>1404_bases
ATGCCTGGTGAGACACGGGGTCCCTCCGTCGGTCCGTCGGTCGCCGAAATCCTGGCGTCCCACGCCGGTGGTACCGGTTC
GCCGACCAAGACCGCGGCGCGGGTGGCCGACGCGATCGCGGCGCGCGGCGACGACGGCACCTGGCTGTCGACGGTGCCCC
GAGAGGAACTGCTCGCCGCCGCCGAGGCCGTCGAGAATCGTCCCGGCGCCCGCACCCTGCCGTTGTACGGGGTGCCGTTC
GGCGTCAAGGACAGCATCGACGTCGCGGGCGTGCCGACGACCCTGTCGTGTCCGGACTACGCCTACGTCGCGACCGCGAC
CGCTCCGGCGGTGCGCCGGCTGCTCGATGCCGGAGCGCTGTATGTCGGCAAGACCAACCTCGACCAGTTCGCCACCGGGC
TCAACGGCACCCGCACCCCCTACACCGTCCCGCGCAGCGTCTACGGCGGTGAGCTGATCTCCGGCGGCTCGAGTTCCGGC
TCGGCGCTGGCCGTCGCTCTCGGACAGGTGCCGTTCGCCGTCGCGACCGACACCGCAGGGTCCGGTCGGGTGCCCGCAGC
GCTGAACGGGGTGATCGGTTACAAACCGTCGCGTGGGCTGATCAGCACGGTGGGGCTGGTACCGGCGTGCAAGTCACTCG
ACTGCCTCAGCGTGATGGCCGGGTGCATCGACGACGTGGATCGCGTAGCCGATGTGATGATCGCCCGCGACGACGCCGAC
CCCTGGTCGCGCGACCGCGGCCCGCGCCACGACGGCTCGGCGATCCGCGTGGGGCTGCCGCCCGTCGAACAGTTGGAGTT
CTTCGGCGACCACGCGATGCGCGACGCCCACCTGCGCTTCCGGGACCACCTCGCCCGCCGGCACACGGTGGTCGACGTAT
CGCTCGAACCGTTTCTGGCCGCCGGCGCACTGCTCTACCAGGGGCCATGGGTGGCGGAGCGGCTGGTGGAGTTCGGCGAT
TTCCTTGCCGGGCAACCTGATTCGATTCACCCGGTGGTCCGGGACATCCTGCGCGGCGGTCAGCGCTATACCGCGGTCGA
CGCATTCGCGGCGCTGCAGGCACTCCAGGAACTCAAGGCGGTCGTCGGGCGACAGTGGCAGCAGATGGATGTGCTCGTCG
TGCCGACCATCGGGACCACCTTCACCGTCGACGAGGTTCTCGAGCGGCCGATCGAGACGAATACGGTGCTCGGGCACTAC
ACCCATTTCGGAAATCTGCTCGACCTGCTGGCCGTCGCGGTGCCGGTGGGTTCGACCGCCGACGGCAGACCGGCCGGCGC
GATGCTGCTGGGCGCTGCGTCGACCGACGACCTCGTCCTGCACCTCGCGGCACAACTACTCGACGAGCCGCGCGAGCGGT
CCGTCCCTGCCGAGTCAGCCGAACCCGCCGAGGAGCACGTGTGA

Upstream 100 bases:

>100_bases
ACGTTCCAGCGGCCGGTGTCGAATCCTCGGTGCGGCGCCCTAACACGATCGACACACGACCGACGCGCGAACGGCATCTG
GCTGCCGACAATCAGCAGAT

Downstream 100 bases:

>100_bases
GCGAGACCATCGACGTCCCCGCTGAGCCGACACCGTTCCGTCTGGTGGCGGGCAAGACCGCGCTGATCGTGATCGACATG
CAGCGTGACTTCCTGTTGCC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MPGETRGPSVGPSVAEILASHAGGTGSPTKTAARVADAIAARGDDGTWLSTVPREELLAAAEAVENRPGARTLPLYGVPF
GVKDSIDVAGVPTTLSCPDYAYVATATAPAVRRLLDAGALYVGKTNLDQFATGLNGTRTPYTVPRSVYGGELISGGSSSG
SALAVALGQVPFAVATDTAGSGRVPAALNGVIGYKPSRGLISTVGLVPACKSLDCLSVMAGCIDDVDRVADVMIARDDAD
PWSRDRGPRHDGSAIRVGLPPVEQLEFFGDHAMRDAHLRFRDHLARRHTVVDVSLEPFLAAGALLYQGPWVAERLVEFGD
FLAGQPDSIHPVVRDILRGGQRYTAVDAFAALQALQELKAVVGRQWQQMDVLVVPTIGTTFTVDEVLERPIETNTVLGHY
THFGNLLDLLAVAVPVGSTADGRPAGAMLLGAASTDDLVLHLAAQLLDEPRERSVPAESAEPAEEHV

Sequences:

>Translated_467_residues
MPGETRGPSVGPSVAEILASHAGGTGSPTKTAARVADAIAARGDDGTWLSTVPREELLAAAEAVENRPGARTLPLYGVPF
GVKDSIDVAGVPTTLSCPDYAYVATATAPAVRRLLDAGALYVGKTNLDQFATGLNGTRTPYTVPRSVYGGELISGGSSSG
SALAVALGQVPFAVATDTAGSGRVPAALNGVIGYKPSRGLISTVGLVPACKSLDCLSVMAGCIDDVDRVADVMIARDDAD
PWSRDRGPRHDGSAIRVGLPPVEQLEFFGDHAMRDAHLRFRDHLARRHTVVDVSLEPFLAAGALLYQGPWVAERLVEFGD
FLAGQPDSIHPVVRDILRGGQRYTAVDAFAALQALQELKAVVGRQWQQMDVLVVPTIGTTFTVDEVLERPIETNTVLGHY
THFGNLLDLLAVAVPVGSTADGRPAGAMLLGAASTDDLVLHLAAQLLDEPRERSVPAESAEPAEEHV
>Mature_466_residues
PGETRGPSVGPSVAEILASHAGGTGSPTKTAARVADAIAARGDDGTWLSTVPREELLAAAEAVENRPGARTLPLYGVPFG
VKDSIDVAGVPTTLSCPDYAYVATATAPAVRRLLDAGALYVGKTNLDQFATGLNGTRTPYTVPRSVYGGELISGGSSSGS
ALAVALGQVPFAVATDTAGSGRVPAALNGVIGYKPSRGLISTVGLVPACKSLDCLSVMAGCIDDVDRVADVMIARDDADP
WSRDRGPRHDGSAIRVGLPPVEQLEFFGDHAMRDAHLRFRDHLARRHTVVDVSLEPFLAAGALLYQGPWVAERLVEFGDF
LAGQPDSIHPVVRDILRGGQRYTAVDAFAALQALQELKAVVGRQWQQMDVLVVPTIGTTFTVDEVLERPIETNTVLGHYT
HFGNLLDLLAVAVPVGSTADGRPAGAMLLGAASTDDLVLHLAAQLLDEPRERSVPAESAEPAEEHV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=28.5148514851485, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI195972892, Length=193, Percent_Identity=27.979274611399, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=407, Percent_Identity=26.5356265356265, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17537465, Length=157, Percent_Identity=31.8471337579618, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=406, Percent_Identity=42.8571428571429, Blast_Score=281, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6323950, Length=187, Percent_Identity=35.2941176470588, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648113, Length=267, Percent_Identity=32.5842696629214, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24652985, Length=424, Percent_Identity=24.0566037735849, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19922090, Length=424, Percent_Identity=24.0566037735849, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24652981, Length=424, Percent_Identity=24.0566037735849, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24652983, Length=424, Percent_Identity=24.0566037735849, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21356731, Length=182, Percent_Identity=31.8681318681319, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 48937; Mature: 48806

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGETRGPSVGPSVAEILASHAGGTGSPTKTAARVADAIAARGDDGTWLSTVPREELLAA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHH
AEAVENRPGARTLPLYGVPFGVKDSIDVAGVPTTLSCPDYAYVATATAPAVRRLLDAGAL
HHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCEECCCCCEEEEHHHHHHHHHHHHCCCE
YVGKTNLDQFATGLNGTRTPYTVPRSVYGGELISGGSSSGSALAVALGQVPFAVATDTAG
EEECCCHHHHHHCCCCCCCCCCCCCHHCCCCEECCCCCCCCEEEEEECCCCEEEEECCCC
SGRVPAALNGVIGYKPSRGLISTVGLVPACKSLDCLSVMAGCIDDVDRVADVMIARDDAD
CCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PWSRDRGPRHDGSAIRVGLPPVEQLEFFGDHAMRDAHLRFRDHLARRHTVVDVSLEPFLA
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEHHHHHH
AGALLYQGPWVAERLVEFGDFLAGQPDSIHPVVRDILRGGQRYTAVDAFAALQALQELKA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VVGRQWQQMDVLVVPTIGTTFTVDEVLERPIETNTVLGHYTHFGNLLDLLAVAVPVGSTA
HHCCCHHHCCEEEECCCCCEEEHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCC
DGRPAGAMLLGAASTDDLVLHLAAQLLDEPRERSVPAESAEPAEEHV
CCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHCC
>Mature Secondary Structure 
PGETRGPSVGPSVAEILASHAGGTGSPTKTAARVADAIAARGDDGTWLSTVPREELLAA
CCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHH
AEAVENRPGARTLPLYGVPFGVKDSIDVAGVPTTLSCPDYAYVATATAPAVRRLLDAGAL
HHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCEECCCCCEEEEHHHHHHHHHHHHCCCE
YVGKTNLDQFATGLNGTRTPYTVPRSVYGGELISGGSSSGSALAVALGQVPFAVATDTAG
EEECCCHHHHHHCCCCCCCCCCCCCHHCCCCEECCCCCCCCEEEEEECCCCEEEEECCCC
SGRVPAALNGVIGYKPSRGLISTVGLVPACKSLDCLSVMAGCIDDVDRVADVMIARDDAD
CCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PWSRDRGPRHDGSAIRVGLPPVEQLEFFGDHAMRDAHLRFRDHLARRHTVVDVSLEPFLA
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEHHHHHH
AGALLYQGPWVAERLVEFGDFLAGQPDSIHPVVRDILRGGQRYTAVDAFAALQALQELKA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VVGRQWQQMDVLVVPTIGTTFTVDEVLERPIETNTVLGHYTHFGNLLDLLAVAVPVGSTA
HHCCCHHHCCEEEECCCCCEEEHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCC
DGRPAGAMLLGAASTDDLVLHLAAQLLDEPRERSVPAESAEPAEEHV
CCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA