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The map label for this gene is hdhA [C]

Identifier: 119868827

GI number: 119868827

Start: 2914267

End: 2915190

Strand: Direct

Name: hdhA [C]

Synonym: Mkms_2795

Alternate gene names: 119868827

Gene position: 2914267-2915190 (Clockwise)

Preceding gene: 119868825

Following gene: 119868828

Centisome position: 50.8

GC content: 69.37

Gene sequence:

>924_bases
ATGACATGGCGCCCCGATCCGGATGCGTTACGTGACCGCATCGCGGTGGTGGCGGGGGCGACCCGCGGCGCCGGGCGCGG
GATAGCCGCCGCCCTCGGCGAGGCGGGTGCCACCGTCATCTGCACTGGACGCAACAGCAGGACCGGACCTGGCGGATCCG
ACTACGACCGGCCGGAGACGATCGAGGAGACCGCCGAACTCGTCACCGCTCTCGGCGGTCGCGGCATCGCCTACCGCGTC
GACCACCTCGACGCCGAACAGGTGCGCACGTTGGCCGACAGGCTGGGCAGCGAGCACGGCCGGATCGACATCCTGGTCAA
CGACATCTGGGGTGCCGAGGTGCTTAAGGGCCCGCCGCCGACGTGGGGCCGCCCGATGTGGGAGCACGATCTGGCCGACG
GACTACGCATCGTGCGGCTCGGACTGGAGACCCATCTCATCACCTCGCACGCCCTGCTGCCGTTGCTGACCGCCCGGGCC
GGCGGCCTGCTCGTCGAGATCACTGACGGCACAACGGAATACAATGCGGCACACTACCGGATCTCGGTGTTCTACGACCT
GGTCAAGTGTGCCGTCAACCGGCTGGCGTTCAGTCACGGACACGAACTGGCGCCCTTCGGTGCCGCCGCGGTCGCCGTGA
GCCCGGGGTGGCTGCGTTCGGAGATGATGCTCGACAATTACGGCGTCACCGAAGCCGACTGGCGTACGGCGCTGAACGCC
GACCGCGCGGACGGCCGCCCGACGGCCCCGCCCGGCTTCGCGGAGTCCGAGACGCCGCGGTATGTGGGCCGCGCCGTCGC
GGCGATCGCCGCCGACTCTGATCGTCAGCGGTGGAATCAGCGCTCGCTGACCTCGGCCGAACTCGCCCGCGAGTACGGAT
TCACCGACATGGATCAACGCCAACCGGACTCATGGACCGAGTGA

Upstream 100 bases:

>100_bases
TCTCTTCGGCGATGTAGCGCTGCAGCGGTGTCACGTTCGCGAGTATGCGCTCATCGCCGAAGCGGCGGAACCGATTGCAT
CACCGCGCCATACTCGCGTC

Downstream 100 bases:

>100_bases
ACACGCAGTTCAGAGCTGAAATAGACAGATCAGGAAATCTGTTCACTGGAATCGTTGACACCCGCCGCACCGGGACTGTT
CTATGACAGTCGTGACCTAC

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: Short Chain Dehydrogenase; Short Chain Dehydrogenase/Reductase Family Oxidoreductase; Oxidoreductase; Short-Chain Dehydrogenase; Oxidoreductase Short Chain Dehydrogenase/Reductase Family; 3-Oxoacyl-[Acyl-Carrier-Protein] Reductase; 3-Ketoacyl-Acyl Carrier; Glucose/Ribitol Dehydrogenase; Dehydrogenase/Reductase SDR Family; Dehydrogenase; 2-Hydroxycyclohexanecarboxyl-CoA Dehydrogenase

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTWRPDPDALRDRIAVVAGATRGAGRGIAAALGEAGATVICTGRNSRTGPGGSDYDRPETIEETAELVTALGGRGIAYRV
DHLDAEQVRTLADRLGSEHGRIDILVNDIWGAEVLKGPPPTWGRPMWEHDLADGLRIVRLGLETHLITSHALLPLLTARA
GGLLVEITDGTTEYNAAHYRISVFYDLVKCAVNRLAFSHGHELAPFGAAAVAVSPGWLRSEMMLDNYGVTEADWRTALNA
DRADGRPTAPPGFAESETPRYVGRAVAAIAADSDRQRWNQRSLTSAELAREYGFTDMDQRQPDSWTE

Sequences:

>Translated_307_residues
MTWRPDPDALRDRIAVVAGATRGAGRGIAAALGEAGATVICTGRNSRTGPGGSDYDRPETIEETAELVTALGGRGIAYRV
DHLDAEQVRTLADRLGSEHGRIDILVNDIWGAEVLKGPPPTWGRPMWEHDLADGLRIVRLGLETHLITSHALLPLLTARA
GGLLVEITDGTTEYNAAHYRISVFYDLVKCAVNRLAFSHGHELAPFGAAAVAVSPGWLRSEMMLDNYGVTEADWRTALNA
DRADGRPTAPPGFAESETPRYVGRAVAAIAADSDRQRWNQRSLTSAELAREYGFTDMDQRQPDSWTE
>Mature_306_residues
TWRPDPDALRDRIAVVAGATRGAGRGIAAALGEAGATVICTGRNSRTGPGGSDYDRPETIEETAELVTALGGRGIAYRVD
HLDAEQVRTLADRLGSEHGRIDILVNDIWGAEVLKGPPPTWGRPMWEHDLADGLRIVRLGLETHLITSHALLPLLTARAG
GLLVEITDGTTEYNAAHYRISVFYDLVKCAVNRLAFSHGHELAPFGAAAVAVSPGWLRSEMMLDNYGVTEADWRTALNAD
RADGRPTAPPGFAESETPRYVGRAVAAIAADSDRQRWNQRSLTSAELAREYGFTDMDQRQPDSWTE

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI209862901, Length=293, Percent_Identity=29.0102389078498, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI19923983, Length=293, Percent_Identity=29.0102389078498, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17555612, Length=303, Percent_Identity=33.993399339934, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71997099, Length=303, Percent_Identity=30.3630363036304, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI86565303, Length=296, Percent_Identity=29.3918918918919, Blast_Score=117, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 33309; Mature: 33177

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWRPDPDALRDRIAVVAGATRGAGRGIAAALGEAGATVICTGRNSRTGPGGSDYDRPET
CCCCCCHHHHHHHHHHEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHH
IEETAELVTALGGRGIAYRVDHLDAEQVRTLADRLGSEHGRIDILVNDIWGAEVLKGPPP
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCCCC
TWGRPMWEHDLADGLRIVRLGLETHLITSHALLPLLTARAGGLLVEITDGTTEYNAAHYR
CCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEH
ISVFYDLVKCAVNRLAFSHGHELAPFGAAAVAVSPGWLRSEMMLDNYGVTEADWRTALNA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHCCC
DRADGRPTAPPGFAESETPRYVGRAVAAIAADSDRQRWNQRSLTSAELAREYGFTDMDQR
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCCCCCCCC
QPDSWTE
CCCCCCC
>Mature Secondary Structure 
TWRPDPDALRDRIAVVAGATRGAGRGIAAALGEAGATVICTGRNSRTGPGGSDYDRPET
CCCCCHHHHHHHHHHEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHH
IEETAELVTALGGRGIAYRVDHLDAEQVRTLADRLGSEHGRIDILVNDIWGAEVLKGPPP
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCCCC
TWGRPMWEHDLADGLRIVRLGLETHLITSHALLPLLTARAGGLLVEITDGTTEYNAAHYR
CCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEH
ISVFYDLVKCAVNRLAFSHGHELAPFGAAAVAVSPGWLRSEMMLDNYGVTEADWRTALNA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHCCC
DRADGRPTAPPGFAESETPRYVGRAVAAIAADSDRQRWNQRSLTSAELAREYGFTDMDQR
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCCCCCCCC
QPDSWTE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA