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The map label for this gene is yyaE [H]

Identifier: 119868797

GI number: 119868797

Start: 2886970

End: 2889228

Strand: Direct

Name: yyaE [H]

Synonym: Mkms_2765

Alternate gene names: 119868797

Gene position: 2886970-2889228 (Clockwise)

Preceding gene: 119868796

Following gene: 119868799

Centisome position: 50.32

GC content: 68.88

Gene sequence:

>2259_bases
GTGACGACCACACCGCTGAAGGGCGACTGGCAGCCGACCGCGTGCATCCTGTGCGAGTGCAACTGCGGCATCGTCGTGGA
GGTCGAAGGCCGCTCACTGGCCCGCATTCGCGGCGACAAAAACCATCCGGCGTCGCAGGGCTACACCTGCAACAAGGCGC
TGCGCCTCGACCACTACCAGAACAACCGCGGCCGGCTGACCTCACCGATGCGCCGCCGCCCCGACGGCAGCTACGAAGAA
ATCGACTGGGACACCGCGATCTCCGAGATCGCCACGGGATTCCGGCGCATCGCCGACACCCACGGCGGGGACAAGATCTT
CTACTACGGCGGTGGTGGGCAGGGCAATCACCTCGGCGGTGCATACAGCGGCGCGTTTCTCAAGGCGCTCGGCTCGTACT
ACCGATCTAACGCGCTGGCACAGGAGAAGACCGGTGAGCACTGGGTGGACGCGCACCTCTACGGCGGGCACACCCGCGGC
GAGTTCGAACACGCCGAGGTGTCGGTGTTCGTCGGGAAGAACCCGTGGATGTCGCAGAGTTTCCCGCGAGCCCGCGTCGT
GCTCAACGAGATCGCCAAGGATCCGAGCCGGTCGATGATCGTCATCGACCCGGTCATCACCGACACCGCCAAGATGGCCG
ACTTCCACCTGCGGGTCCGTCCGGGCACCGATGCGTGGTGTCTGGCGGCGATGGCCGCGGTGCTCGTGCAGGAGGACCTG
TGCGACGAGACGTTCCTGGCGGCCCATGTCAACGGCGCCGACGCCGTGCGCGAGGTGTTGCGCGAGGTGCCGGTGGCCGA
ATTCGCGCGGCGCTGTGGCGTCTCCGAAGACCTGCTGCGGGCCGCGGTGCGCCGTATCGCGGCCGCCGAGAGCGTCTCGG
TGTTCGAGGACCTCGGAATTCAGCAGTCCCCCAACAGCACCGTGTGCTCGTATCTGAACAAGTTGCTGTGGATCCTCACC
GGCAACTTCGCCAAACGCGGTGCCCAGCATCTACATTCGACGTTCGCTCCGCTGTTCCGCGCCGGTGGTGTCGGCCGCAC
ACCCGTGACGGGCGCCCCGGTGATCGGCGGGCTGGTACCGAGCAACGTGGTGCCCCAGGAGATCCTGACCGACCACCCGG
ACCGGTTCCGGGCCATGGTCGTCGAGAGCAGCAATCCGGCCCACTCCCTCGCCGATTCGGCCGCGTGTCGCGAGGCGTTC
TCGGCGCTCGAGCTCCTGGTGGTCGTCGACGTCGCGATGACCGAGACCGCACGGCTGGCCCACTACGTGCTGCCCGCCGC
GACGCAGTTCGAGAAGGCCGAGGCGACCTTCTTCAATCTCGAGTTCCCGCACAACACGTTCCACCTGCGGCACCGGCTGT
TCGAGCCGCTCGAGGGCACGCTGCCCGAACCCGAGATCTGGGCCCGGCTGACCCGTGCGCTCGGAGTGGTCGACGACGCC
GAACTCGCACCGCTGCGCGCGGCCGCACGCGACGGGCTGGCCGCCTACAGCACGGCGTTCCTCACCGCCCTGGGCGCCAA
TCCCGGTTTGAACAAGGTCCTGCCCTACGTGCTGTACGAGACGCTCGGCCCGGCGCTGCCGGAAGGCCTGGCCGGGGCGG
CGGCGTTGTGGGGGCTGGCGCAGAAGACCGCGATGACCTATCCCGAGGCGGTGCGACGGGCCGGCCATGCCGACGGCAAC
GCGCTGTTCGAGGCGATCCTGAACCAACGCTCGGGAGTCACCTTCACCGTCCACGAGTATCCGGACGATTTCGACCTGAT
CACCCACGACGACCACCGGATCGCTCTGGACATGCCGGACATGCTCGGCGAGGTCAGGGCCCTCACCGCCGAGCGTGAGG
CACTCACATCGGCCGAGTTCCCGATCGTGCTCTCTGCCGGTGAGCGCCGGGCGTACACGGCCAACGACATCCTGCGGGAT
CCGGGATGGCGCAAACGCGACGCCGACGGCGCCCTGCGGATCAGTGTCGAAGACGCCGCCGCCCTGAACCTGCGGACCGG
TGACCGGGCGCGGATCACCACCGCGGCCGGAAGCGCCGAGGCCGGCGTGGAGGTCAGCGAGGCGATGCTGCCGGGCCACG
CCTCGCTGCCCAACGGCTTCGGGCTGGACTACCTCGACGCCGACGGCACCACCCGGGTCCCCGGTGTGGCCCCGAACTCG
CTCACGTCCAGTGACTGGCGCGACGCCTACGCCGGGACCCCGTGGCACAAGCACGTACCGGCGCGCATCGAGACCGTGAG
CTCCGCCGTCGGCGGCTGA

Upstream 100 bases:

>100_bases
GGATCGTCGTGGCGCGTGCCGCCCGCGACGTCGGCCCCCTCGACGTCATCCACCGCCAACTGCGCACCCTGCTGCAGGAC
GCCCTCGGGAAGGACCGCTC

Downstream 100 bases:

>100_bases
TCCCCGCTACGGCACGGGTGCCGGGGCAGGGGCAGGTGCGGGTGCGGGTGGCGGCACCGGCTCGGGCGCCGGAGCCACCG
GCGCCTGCGGTCCGGCGGGC

Product: formate dehydrogenase

Products: nitrate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 752; Mature: 751

Protein sequence:

>752_residues
MTTTPLKGDWQPTACILCECNCGIVVEVEGRSLARIRGDKNHPASQGYTCNKALRLDHYQNNRGRLTSPMRRRPDGSYEE
IDWDTAISEIATGFRRIADTHGGDKIFYYGGGGQGNHLGGAYSGAFLKALGSYYRSNALAQEKTGEHWVDAHLYGGHTRG
EFEHAEVSVFVGKNPWMSQSFPRARVVLNEIAKDPSRSMIVIDPVITDTAKMADFHLRVRPGTDAWCLAAMAAVLVQEDL
CDETFLAAHVNGADAVREVLREVPVAEFARRCGVSEDLLRAAVRRIAAAESVSVFEDLGIQQSPNSTVCSYLNKLLWILT
GNFAKRGAQHLHSTFAPLFRAGGVGRTPVTGAPVIGGLVPSNVVPQEILTDHPDRFRAMVVESSNPAHSLADSAACREAF
SALELLVVVDVAMTETARLAHYVLPAATQFEKAEATFFNLEFPHNTFHLRHRLFEPLEGTLPEPEIWARLTRALGVVDDA
ELAPLRAAARDGLAAYSTAFLTALGANPGLNKVLPYVLYETLGPALPEGLAGAAALWGLAQKTAMTYPEAVRRAGHADGN
ALFEAILNQRSGVTFTVHEYPDDFDLITHDDHRIALDMPDMLGEVRALTAEREALTSAEFPIVLSAGERRAYTANDILRD
PGWRKRDADGALRISVEDAAALNLRTGDRARITTAAGSAEAGVEVSEAMLPGHASLPNGFGLDYLDADGTTRVPGVAPNS
LTSSDWRDAYAGTPWHKHVPARIETVSSAVGG

Sequences:

>Translated_752_residues
MTTTPLKGDWQPTACILCECNCGIVVEVEGRSLARIRGDKNHPASQGYTCNKALRLDHYQNNRGRLTSPMRRRPDGSYEE
IDWDTAISEIATGFRRIADTHGGDKIFYYGGGGQGNHLGGAYSGAFLKALGSYYRSNALAQEKTGEHWVDAHLYGGHTRG
EFEHAEVSVFVGKNPWMSQSFPRARVVLNEIAKDPSRSMIVIDPVITDTAKMADFHLRVRPGTDAWCLAAMAAVLVQEDL
CDETFLAAHVNGADAVREVLREVPVAEFARRCGVSEDLLRAAVRRIAAAESVSVFEDLGIQQSPNSTVCSYLNKLLWILT
GNFAKRGAQHLHSTFAPLFRAGGVGRTPVTGAPVIGGLVPSNVVPQEILTDHPDRFRAMVVESSNPAHSLADSAACREAF
SALELLVVVDVAMTETARLAHYVLPAATQFEKAEATFFNLEFPHNTFHLRHRLFEPLEGTLPEPEIWARLTRALGVVDDA
ELAPLRAAARDGLAAYSTAFLTALGANPGLNKVLPYVLYETLGPALPEGLAGAAALWGLAQKTAMTYPEAVRRAGHADGN
ALFEAILNQRSGVTFTVHEYPDDFDLITHDDHRIALDMPDMLGEVRALTAEREALTSAEFPIVLSAGERRAYTANDILRD
PGWRKRDADGALRISVEDAAALNLRTGDRARITTAAGSAEAGVEVSEAMLPGHASLPNGFGLDYLDADGTTRVPGVAPNS
LTSSDWRDAYAGTPWHKHVPARIETVSSAVGG
>Mature_751_residues
TTTPLKGDWQPTACILCECNCGIVVEVEGRSLARIRGDKNHPASQGYTCNKALRLDHYQNNRGRLTSPMRRRPDGSYEEI
DWDTAISEIATGFRRIADTHGGDKIFYYGGGGQGNHLGGAYSGAFLKALGSYYRSNALAQEKTGEHWVDAHLYGGHTRGE
FEHAEVSVFVGKNPWMSQSFPRARVVLNEIAKDPSRSMIVIDPVITDTAKMADFHLRVRPGTDAWCLAAMAAVLVQEDLC
DETFLAAHVNGADAVREVLREVPVAEFARRCGVSEDLLRAAVRRIAAAESVSVFEDLGIQQSPNSTVCSYLNKLLWILTG
NFAKRGAQHLHSTFAPLFRAGGVGRTPVTGAPVIGGLVPSNVVPQEILTDHPDRFRAMVVESSNPAHSLADSAACREAFS
ALELLVVVDVAMTETARLAHYVLPAATQFEKAEATFFNLEFPHNTFHLRHRLFEPLEGTLPEPEIWARLTRALGVVDDAE
LAPLRAAARDGLAAYSTAFLTALGANPGLNKVLPYVLYETLGPALPEGLAGAAALWGLAQKTAMTYPEAVRRAGHADGNA
LFEAILNQRSGVTFTVHEYPDDFDLITHDDHRIALDMPDMLGEVRALTAEREALTSAEFPIVLSAGERRAYTANDILRDP
GWRKRDADGALRISVEDAAALNLRTGDRARITTAAGSAEAGVEVSEAMLPGHASLPNGFGLDYLDADGTTRVPGVAPNSL
TSSDWRDAYAGTPWHKHVPARIETVSSAVGG

Specific function: Decomposes Formic Acid To Hydrogen And Carbon Dioxide Under Anaerobic Conditions In The Absence Of Exogenous Electron Acceptors. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=469, Percent_Identity=25.5863539445629, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1787870, Length=488, Percent_Identity=26.2295081967213, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI87081797, Length=484, Percent_Identity=23.7603305785124, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI171474008, Length=357, Percent_Identity=26.6106442577031, Blast_Score=90, Evalue=7e-19,
Organism=Escherichia coli, GI3868719, Length=275, Percent_Identity=25.0909090909091, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788534, Length=260, Percent_Identity=23.4615384615385, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.7.99.4

Molecular weight: Translated: 81386; Mature: 81255

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPLKGDWQPTACILCECNCGIVVEVEGRSLARIRGDKNHPASQGYTCNKALRLDHYQ
CCCCCCCCCCCCCEEEEEECCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEECCCC
NNRGRLTSPMRRRPDGSYEEIDWDTAISEIATGFRRIADTHGGDKIFYYGGGGQGNHLGG
CCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
AYSGAFLKALGSYYRSNALAQEKTGEHWVDAHLYGGHTRGEFEHAEVSVFVGKNPWMSQS
HHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCC
FPRARVVLNEIAKDPSRSMIVIDPVITDTAKMADFHLRVRPGTDAWCLAAMAAVLVQEDL
CCHHHHHHHHHHCCCCCCEEEECCCCCCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHHH
CDETFLAAHVNGADAVREVLREVPVAEFARRCGVSEDLLRAAVRRIAAAESVSVFEDLGI
CCCCCCEEECCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
QQSPNSTVCSYLNKLLWILTGNFAKRGAQHLHSTFAPLFRAGGVGRTPVTGAPVIGGLVP
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCC
SNVVPQEILTDHPDRFRAMVVESSNPAHSLADSAACREAFSALELLVVVDVAMTETARLA
CCCCCHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HYVLPAATQFEKAEATFFNLEFPHNTFHLRHRLFEPLEGTLPEPEIWARLTRALGVVDDA
HHHHCCHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
ELAPLRAAARDGLAAYSTAFLTALGANPGLNKVLPYVLYETLGPALPEGLAGAAALWGLA
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
QKTAMTYPEAVRRAGHADGNALFEAILNQRSGVTFTVHEYPDDFDLITHDDHRIALDMPD
HHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCCCEEEECCHH
MLGEVRALTAEREALTSAEFPIVLSAGERRAYTANDILRDPGWRKRDADGALRISVEDAA
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEHHHHHCCCCCCCCCCCCEEEEEECCCE
ALNLRTGDRARITTAAGSAEAGVEVSEAMLPGHASLPNGFGLDYLDADGTTRVPGVAPNS
EEEECCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCC
LTSSDWRDAYAGTPWHKHVPARIETVSSAVGG
CCCCCCHHHCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TTTPLKGDWQPTACILCECNCGIVVEVEGRSLARIRGDKNHPASQGYTCNKALRLDHYQ
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEECCCC
NNRGRLTSPMRRRPDGSYEEIDWDTAISEIATGFRRIADTHGGDKIFYYGGGGQGNHLGG
CCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
AYSGAFLKALGSYYRSNALAQEKTGEHWVDAHLYGGHTRGEFEHAEVSVFVGKNPWMSQS
HHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCC
FPRARVVLNEIAKDPSRSMIVIDPVITDTAKMADFHLRVRPGTDAWCLAAMAAVLVQEDL
CCHHHHHHHHHHCCCCCCEEEECCCCCCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHHH
CDETFLAAHVNGADAVREVLREVPVAEFARRCGVSEDLLRAAVRRIAAAESVSVFEDLGI
CCCCCCEEECCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
QQSPNSTVCSYLNKLLWILTGNFAKRGAQHLHSTFAPLFRAGGVGRTPVTGAPVIGGLVP
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCC
SNVVPQEILTDHPDRFRAMVVESSNPAHSLADSAACREAFSALELLVVVDVAMTETARLA
CCCCCHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HYVLPAATQFEKAEATFFNLEFPHNTFHLRHRLFEPLEGTLPEPEIWARLTRALGVVDDA
HHHHCCHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
ELAPLRAAARDGLAAYSTAFLTALGANPGLNKVLPYVLYETLGPALPEGLAGAAALWGLA
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
QKTAMTYPEAVRRAGHADGNALFEAILNQRSGVTFTVHEYPDDFDLITHDDHRIALDMPD
HHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCCCEEEECCHH
MLGEVRALTAEREALTSAEFPIVLSAGERRAYTANDILRDPGWRKRDADGALRISVEDAA
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEHHHHHCCCCCCCCCCCCEEEEEECCCE
ALNLRTGDRARITTAAGSAEAGVEVSEAMLPGHASLPNGFGLDYLDADGTTRVPGVAPNS
EEEECCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCC
LTSSDWRDAYAGTPWHKHVPARIETVSSAVGG
CCCCCCHHHCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite; acceptor

Specific reaction: nitrite + acceptor = nitrate + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1552862 [H]