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The map label for this gene is hpaH [H]

Identifier: 119868694

GI number: 119868694

Start: 2793293

End: 2794651

Strand: Direct

Name: hpaH [H]

Synonym: Mkms_2661

Alternate gene names: 119868694

Gene position: 2793293-2794651 (Clockwise)

Preceding gene: 119868693

Following gene: 119868695

Centisome position: 48.69

GC content: 64.31

Gene sequence:

>1359_bases
ATGCAGCATGATCCGCGTTACCAGGATCAGCTGACCTTCACCTCCCCCACCACCGGTGACCTGGTCAACGCTTCCTTCCT
GGTGCCCCGTTCGGTCGAGGATCTCAAGCGCCGCCGCGCTGCGATCCAGACATGGGCCGACGAGACCAACGGTTTCCTTG
GGCGCAGCGGGGACTACATGAACAGCTCGCTCACAGCGCTCGGAGCCGCGCAGACATGGTTCGCCAAGGCCGACCCCAAG
TTCGGTGAGAGAATCACCGCCTACTACGAATACGCGCGGGAGAACGACCTGCTGGCCACCCACACGTTGATACCGCCGCA
GGTCAACCGATCCTTGACCGGCAGCCAGCAGGGTGGTGGACAGCTCGCCGCACGGATCGTCGAGGAGCGGCCGGACGGCA
TCGTCGTGCGCGGTGCCCGGATGCTGGCCACCGTGGCTCCGATCGCCGACGAGCTGCTGGTCTTCCCCTCCACCGTGTTA
CGCGGAACACCCGAGGACGCGCCGTACAGCTACGCGTTCGCGATCCAGAACGACGCACCCGGCTTGCGCTACCTCTGCCG
GGGTTCACTTCACAACGGCGGCAGCACTTTTGACGAGCCGTTGGCCGCCCGGTATGAGGAGATGGATGCGGTGGTGGTTT
TCGACGACGTCTTCGTGCCAAATGACCGCGTCTTCATGCTCGGACACCCCGATTTGTGCAACGCCTTCTACGCCGAAACC
GGCGCGGGGGCGTTGATGACCCATCAGGTCGTGACGCGCACCGTCGCCAAGACCGAGTTCTTCCTCGGTCTGGCGTCGGA
GATCGGTGCCGCGATCGGAATCGAAGGCTTCCAGCACATTCAGGAGGACCTGGCCGAACTCATCGTCGACCTCGAAATCG
GGAGGGCGTTGCTCCGCGCGGCCGAAGCCGACGGAGCCATCAACGACTGGGGTGTGTACCTGCCGCGGTGGGAAACTCTC
AACGCCGCTCGCAACTGGTATCCGAAAGTGTCACAACGCTTTCCGCAGATCATTCGCAAGTTCAGCGCCTCCGGCCTGAT
GGCGCTGCCCGGTGAGGCCGACATCGCCTCGGATGCCCGCGCCGACATCGAGCAATACCTGCAAGGCGCTACGGTCAGCG
GTACCGACCGGGTCCAGCTGTTCAAGCTGGCCTTCGACGCCTCGATTTCCGGGTTCTCCAGCCGGCAAGCGCTCTACGAG
TACTTCTTCTTCGGCGACCCGGTACGTATGGCCGGGGCTCTGGTGAACGGCTACGACCGTGACCCGGCCCGCGAGCGGAT
CCGAAAGCTGCTGAACGAAGGTCGAGCGAATCTGCCCCACGACACGTGCAGCGAGCGCGTCGCCACACTGGCCCTCTGA

Upstream 100 bases:

>100_bases
TGCGCCCCCACATCGTGATCGACGGTGAGGTGGTCAGCGAGAACGTCGCGGAGCATCCGGCGTTCCGTGATGTCGCCGTA
CTTACGCGAAACTGTTTGAC

Downstream 100 bases:

>100_bases
TGCGGCCAGCCCCTTTGATTGGTCAACAGAAAGCGCGCAGATGACACACTTCCGCCCCAACGTCCGAGATATCGAGTTCA
ACCTTTTCGAGGTCCTCGGT

Product: 4-hydroxyphenylacetate 3-monooxygenase, oxygenase subunit

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-hydroxyphenylacetate-3-hydroxylase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MQHDPRYQDQLTFTSPTTGDLVNASFLVPRSVEDLKRRRAAIQTWADETNGFLGRSGDYMNSSLTALGAAQTWFAKADPK
FGERITAYYEYARENDLLATHTLIPPQVNRSLTGSQQGGGQLAARIVEERPDGIVVRGARMLATVAPIADELLVFPSTVL
RGTPEDAPYSYAFAIQNDAPGLRYLCRGSLHNGGSTFDEPLAARYEEMDAVVVFDDVFVPNDRVFMLGHPDLCNAFYAET
GAGALMTHQVVTRTVAKTEFFLGLASEIGAAIGIEGFQHIQEDLAELIVDLEIGRALLRAAEADGAINDWGVYLPRWETL
NAARNWYPKVSQRFPQIIRKFSASGLMALPGEADIASDARADIEQYLQGATVSGTDRVQLFKLAFDASISGFSSRQALYE
YFFFGDPVRMAGALVNGYDRDPARERIRKLLNEGRANLPHDTCSERVATLAL

Sequences:

>Translated_452_residues
MQHDPRYQDQLTFTSPTTGDLVNASFLVPRSVEDLKRRRAAIQTWADETNGFLGRSGDYMNSSLTALGAAQTWFAKADPK
FGERITAYYEYARENDLLATHTLIPPQVNRSLTGSQQGGGQLAARIVEERPDGIVVRGARMLATVAPIADELLVFPSTVL
RGTPEDAPYSYAFAIQNDAPGLRYLCRGSLHNGGSTFDEPLAARYEEMDAVVVFDDVFVPNDRVFMLGHPDLCNAFYAET
GAGALMTHQVVTRTVAKTEFFLGLASEIGAAIGIEGFQHIQEDLAELIVDLEIGRALLRAAEADGAINDWGVYLPRWETL
NAARNWYPKVSQRFPQIIRKFSASGLMALPGEADIASDARADIEQYLQGATVSGTDRVQLFKLAFDASISGFSSRQALYE
YFFFGDPVRMAGALVNGYDRDPARERIRKLLNEGRANLPHDTCSERVATLAL
>Mature_452_residues
MQHDPRYQDQLTFTSPTTGDLVNASFLVPRSVEDLKRRRAAIQTWADETNGFLGRSGDYMNSSLTALGAAQTWFAKADPK
FGERITAYYEYARENDLLATHTLIPPQVNRSLTGSQQGGGQLAARIVEERPDGIVVRGARMLATVAPIADELLVFPSTVL
RGTPEDAPYSYAFAIQNDAPGLRYLCRGSLHNGGSTFDEPLAARYEEMDAVVVFDDVFVPNDRVFMLGHPDLCNAFYAET
GAGALMTHQVVTRTVAKTEFFLGLASEIGAAIGIEGFQHIQEDLAELIVDLEIGRALLRAAEADGAINDWGVYLPRWETL
NAARNWYPKVSQRFPQIIRKFSASGLMALPGEADIASDARADIEQYLQGATVSGTDRVQLFKLAFDASISGFSSRQALYE
YFFFGDPVRMAGALVNGYDRDPARERIRKLLNEGRANLPHDTCSERVATLAL

Specific function: Utilizes FADH(2) supplied by hpaI, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 49748; Mature: 49748

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHDPRYQDQLTFTSPTTGDLVNASFLVPRSVEDLKRRRAAIQTWADETNGFLGRSGDYM
CCCCCCCCCCEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NSSLTALGAAQTWFAKADPKFGERITAYYEYARENDLLATHTLIPPQVNRSLTGSQQGGG
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCH
QLAARIVEERPDGIVVRGARMLATVAPIADELLVFPSTVLRGTPEDAPYSYAFAIQNDAP
HHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GLRYLCRGSLHNGGSTFDEPLAARYEEMDAVVVFDDVFVPNDRVFMLGHPDLCNAFYAET
CHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEEEEECEECCCCCEEEECCCHHHHHHHHHC
GAGALMTHQVVTRTVAKTEFFLGLASEIGAAIGIEGFQHIQEDLAELIVDLEIGRALLRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEADGAINDWGVYLPRWETLNAARNWYPKVSQRFPQIIRKFSASGLMALPGEADIASDAR
HHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHH
ADIEQYLQGATVSGTDRVQLFKLAFDASISGFSSRQALYEYFFFGDPVRMAGALVNGYDR
HHHHHHHCCCCCCCCCHHEEEEEEHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCC
DPARERIRKLLNEGRANLPHDTCSERVATLAL
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQHDPRYQDQLTFTSPTTGDLVNASFLVPRSVEDLKRRRAAIQTWADETNGFLGRSGDYM
CCCCCCCCCCEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NSSLTALGAAQTWFAKADPKFGERITAYYEYARENDLLATHTLIPPQVNRSLTGSQQGGG
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCH
QLAARIVEERPDGIVVRGARMLATVAPIADELLVFPSTVLRGTPEDAPYSYAFAIQNDAP
HHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GLRYLCRGSLHNGGSTFDEPLAARYEEMDAVVVFDDVFVPNDRVFMLGHPDLCNAFYAET
CHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEEEEECEECCCCCEEEECCCHHHHHHHHHC
GAGALMTHQVVTRTVAKTEFFLGLASEIGAAIGIEGFQHIQEDLAELIVDLEIGRALLRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEADGAINDWGVYLPRWETLNAARNWYPKVSQRFPQIIRKFSASGLMALPGEADIASDAR
HHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHH
ADIEQYLQGATVSGTDRVQLFKLAFDASISGFSSRQALYEYFFFGDPVRMAGALVNGYDR
HHHHHHHCCCCCCCCCHHEEEEEEHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCC
DPARERIRKLLNEGRANLPHDTCSERVATLAL
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA