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The map label for this gene is trpB2 [H]

Identifier: 119868669

GI number: 119868669

Start: 2766275

End: 2767576

Strand: Direct

Name: trpB2 [H]

Synonym: Mkms_2635

Alternate gene names: 119868669

Gene position: 2766275-2767576 (Clockwise)

Preceding gene: 119868667

Following gene: 119868670

Centisome position: 48.22

GC content: 68.59

Gene sequence:

>1302_bases
ATGACGGAACAGCTCGACGCCACCCATCCGGACCTCGTCACCGTCGAGGTGCCGACGCACTGGTACAACCTGGCGGCCGA
GCTGGGCGAGCCGATCCCGCCGCATCTGCATCCCGGCACCAAAGAACCGGTCGGCCCGGATGACCTCGCGCCGCTGTTCG
CCAGCGGGTTGATCGCGCAGGAGGTGGCCACCGAGATTTACATCGAGATCCCGCAGGAGGTGCGGGAGATCTACACGATG
TGGCGTCCGTCCCCGCTGATCCGCGCCCGGCGCTTCGAGAAGGCCCTGAACACCAGCGCCCACATCTACGTCAAGTACGA
AGGTGTCAGCCCGGTCGGCAGCCACAAGACCAACTCCGCGGTGGCACAGGCGTACTACAACTCCATCGACGGCGTGCGGA
AGCTCACCACCGAGACCGGCGCCGGGCAGTGGGGAAGTGCCCTGTCGTTCGCCGGAGCCCAGTTCGGACTGGAGGTCGAG
GTGTGGCAGGTCCGCGCTTCGTACGAGTCCAAGCCGTATCGGGGGCATCTGATGCGGACCTACGGCGGCACGGTGCACTC
GAGCCCGTCGACGCTCACCGAGGCGGGCCGCGCCATCCTGGCCAAGGATCCGAACACCACCGGCAGCCTCGGCATGGCGG
TCAGCGAGGCGGTCGAGGTTGCCGCGGCCGACCCGGAGGCCCGCTATGCGCTGGGCAGCGTGCTCAATCATGTGGTGCTG
CATCAGACCGTCATCGGCCAGGAGGCCGTCGCGCAGCTGGCGTTGGTGGAGCCGAACGGCGCCGACGTCGTATTCGGTTG
CGCCGGAGGCGGTTCGAACCTCGCCGGGCTGTCGTTTCCGTTCCTGCGGGAGAAGGTCCACGGCCGGTCGAACCCGCGGA
TCGTGGCCGCAGAGCCAACTGCGTGCCCGTCGATCACCGAGGGTGAGTACCGCTACGACCATGGCGACGTGGCCGGGCTC
ACCCCGCTGCTCAAGATGCACACGCTGGGTATGGATTTCGTGCCCGACCCGATCCACGCCGGCGGACTGCGCTACCACGG
CATGGCGCCGGCGCTGAGCCACACCGTGGAGCTGGGTCTGGTCGAGGGCGTGGCGATCTCGCAGCGCGACGCGTTCGCTG
CCGGGGTGCAGTTCGCGCGAACGCAGGGCATCGTGCCGGCTCCGGAGTCGACGCACGCCATCGCGGCCGCGGCCGCACAT
GTCGCCGATGACCCGCGCGAGCAGGTCGTGGTGATCGGACTGTCCGGGCATGGTCAGCTGGATCTGCCGGCCTACGCGGA
GTACCTCGACGGCAAGCTCTGA

Upstream 100 bases:

>100_bases
AGACGATCCGCGAGATCGTCCACGACATCTTCCTGCCACGGCGACCTCGTCTTGAAAAGCACATTTCAGCCTCACAAACG
CGAGCGTTAGGGTGTCCGGC

Downstream 100 bases:

>100_bases
GCAGGCGGTTTGTGGTCGCGGCTATCCTCGACACCAGGCGTTTCTTGAGGAGGTGAGCCCCGTGTTCCGTCTGTGGTCGC
GCGACCAGTCGATCGAGACG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTEQLDATHPDLVTVEVPTHWYNLAAELGEPIPPHLHPGTKEPVGPDDLAPLFASGLIAQEVATEIYIEIPQEVREIYTM
WRPSPLIRARRFEKALNTSAHIYVKYEGVSPVGSHKTNSAVAQAYYNSIDGVRKLTTETGAGQWGSALSFAGAQFGLEVE
VWQVRASYESKPYRGHLMRTYGGTVHSSPSTLTEAGRAILAKDPNTTGSLGMAVSEAVEVAAADPEARYALGSVLNHVVL
HQTVIGQEAVAQLALVEPNGADVVFGCAGGGSNLAGLSFPFLREKVHGRSNPRIVAAEPTACPSITEGEYRYDHGDVAGL
TPLLKMHTLGMDFVPDPIHAGGLRYHGMAPALSHTVELGLVEGVAISQRDAFAAGVQFARTQGIVPAPESTHAIAAAAAH
VADDPREQVVVIGLSGHGQLDLPAYAEYLDGKL

Sequences:

>Translated_433_residues
MTEQLDATHPDLVTVEVPTHWYNLAAELGEPIPPHLHPGTKEPVGPDDLAPLFASGLIAQEVATEIYIEIPQEVREIYTM
WRPSPLIRARRFEKALNTSAHIYVKYEGVSPVGSHKTNSAVAQAYYNSIDGVRKLTTETGAGQWGSALSFAGAQFGLEVE
VWQVRASYESKPYRGHLMRTYGGTVHSSPSTLTEAGRAILAKDPNTTGSLGMAVSEAVEVAAADPEARYALGSVLNHVVL
HQTVIGQEAVAQLALVEPNGADVVFGCAGGGSNLAGLSFPFLREKVHGRSNPRIVAAEPTACPSITEGEYRYDHGDVAGL
TPLLKMHTLGMDFVPDPIHAGGLRYHGMAPALSHTVELGLVEGVAISQRDAFAAGVQFARTQGIVPAPESTHAIAAAAAH
VADDPREQVVVIGLSGHGQLDLPAYAEYLDGKL
>Mature_432_residues
TEQLDATHPDLVTVEVPTHWYNLAAELGEPIPPHLHPGTKEPVGPDDLAPLFASGLIAQEVATEIYIEIPQEVREIYTMW
RPSPLIRARRFEKALNTSAHIYVKYEGVSPVGSHKTNSAVAQAYYNSIDGVRKLTTETGAGQWGSALSFAGAQFGLEVEV
WQVRASYESKPYRGHLMRTYGGTVHSSPSTLTEAGRAILAKDPNTTGSLGMAVSEAVEVAAADPEARYALGSVLNHVVLH
QTVIGQEAVAQLALVEPNGADVVFGCAGGGSNLAGLSFPFLREKVHGRSNPRIVAAEPTACPSITEGEYRYDHGDVAGLT
PLLKMHTLGMDFVPDPIHAGGLRYHGMAPALSHTVELGLVEGVAISQRDAFAAGVQFARTQGIVPAPESTHAIAAAAAHV
ADDPREQVVVIGLSGHGQLDLPAYAEYLDGKL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=357, Percent_Identity=29.1316526610644, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321412, Length=378, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 46032; Mature: 45901

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQLDATHPDLVTVEVPTHWYNLAAELGEPIPPHLHPGTKEPVGPDDLAPLFASGLIAQ
CCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EVATEIYIEIPQEVREIYTMWRPSPLIRARRFEKALNTSAHIYVKYEGVSPVGSHKTNSA
HHHHHHEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHH
VAQAYYNSIDGVRKLTTETGAGQWGSALSFAGAQFGLEVEVWQVRASYESKPYRGHLMRT
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHCCCEEEEEEEEECCCCCCCCCCEEEE
YGGTVHSSPSTLTEAGRAILAKDPNTTGSLGMAVSEAVEVAAADPEARYALGSVLNHVVL
CCCEECCCCHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
HQTVIGQEAVAQLALVEPNGADVVFGCAGGGSNLAGLSFPFLREKVHGRSNPRIVAAEPT
HHHHHCHHHHHEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCC
ACPSITEGEYRYDHGDVAGLTPLLKMHTLGMDFVPDPIHAGGLRYHGMAPALSHTVELGL
CCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHE
VEGVAISQRDAFAAGVQFARTQGIVPAPESTHAIAAAAAHVADDPREQVVVIGLSGHGQL
ECCEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCC
DLPAYAEYLDGKL
CCHHHHHHHCCCC
>Mature Secondary Structure 
TEQLDATHPDLVTVEVPTHWYNLAAELGEPIPPHLHPGTKEPVGPDDLAPLFASGLIAQ
CCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EVATEIYIEIPQEVREIYTMWRPSPLIRARRFEKALNTSAHIYVKYEGVSPVGSHKTNSA
HHHHHHEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHH
VAQAYYNSIDGVRKLTTETGAGQWGSALSFAGAQFGLEVEVWQVRASYESKPYRGHLMRT
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHCCCEEEEEEEEECCCCCCCCCCEEEE
YGGTVHSSPSTLTEAGRAILAKDPNTTGSLGMAVSEAVEVAAADPEARYALGSVLNHVVL
CCCEECCCCHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
HQTVIGQEAVAQLALVEPNGADVVFGCAGGGSNLAGLSFPFLREKVHGRSNPRIVAAEPT
HHHHHCHHHHHEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCC
ACPSITEGEYRYDHGDVAGLTPLLKMHTLGMDFVPDPIHAGGLRYHGMAPALSHTVELGL
CCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHE
VEGVAISQRDAFAAGVQFARTQGIVPAPESTHAIAAAAAHVADDPREQVVVIGLSGHGQL
ECCEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCC
DLPAYAEYLDGKL
CCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]