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The map label for this gene is lip2 [H]

Identifier: 119868460

GI number: 119868460

Start: 2541835

End: 2542737

Strand: Reverse

Name: lip2 [H]

Synonym: Mkms_2424

Alternate gene names: 119868460

Gene position: 2542737-2541835 (Counterclockwise)

Preceding gene: 119868470

Following gene: 119868458

Centisome position: 44.32

GC content: 72.43

Gene sequence:

>903_bases
GTGCCGTTCCAGTTCTCGGTCGACGAGGGCGCCGAGGTGATTCGCCGCAAGCTGCGTGACCTGCCCCGGCGGGAGGTCCA
TCCCGAGGTCGAGGCGCAGGACCGGACGATCCCGGGGCCCGCGGGCGACATCCCGATCCGCGTGTACCGGCCCGCGACCG
CGGAGTCGTCACCGCCGGTGGTGGTGTTCTTCCACGGCGGCGGGTTCGCCGCCGGAGACCTCGACACCCACGACGGTGAA
TGCCGGCACCACGCGGTGGCCGCCGACGCGGTCGTGGTGGCCGTGGACTACCGGTTGGCGCCCGAACATCCGTACCCGGC
CGCGGTCGAGGATGCGTGGGCGGCGCTGACCTGGGTCGCCGGACACGGCGCCGAACTGTCGGTGGACACGAACCGCCTTG
CGGTGGCCGGCGATTCCGCCGGCGGGAACCTGTCGGCGGTGATGACCCACCGCGCCCGTACGCGGGGCGGACCGCCCATC
GCCTTCCAGCTGCTGTGGTATCCGGTGACGATGTGGGACATGTCGCTGCCGTCGGTGACCGAGAACGCCGGCGCACCGAT
CCTGGACCGCACCGCGATGGACGGGCTGTCGCGCTGGTATGCCGGTGAACTGACCGATCCCGGGCACGAGCTGGCGCCGG
GCCTGGCGGACGACTTCACCGGGCTGCCGCCGGCGTACATCGCGGTCGCGGGGCACGACCCGCTGCGCGACGACGGTATC
CGCTACGCCGAATTGCTCTCCGCCGCAGGCGTTCCGGTCGAAGTGCACAATGCGGAGACGCTGGTGCACGGATACCTCGG
CTACGCGGGTGTGGTGCCCTCGGCGACGGCGGCCACCGACCGTGGGCTGGCGGCGTTGCGGGCGGCGCTACACCCCGAGG
CGGGTCAGCGTCCCGAGAAGTAG

Upstream 100 bases:

>100_bases
CCACCACAGGGCGGTCCGCCGGTGTGCGTACGGTGAGGTCGTGTCTGTGGCTGACGAGAAACCGCCGATCGACGACATTC
TGAGAAAGGTACTGGACGCG

Downstream 100 bases:

>100_bases
TGCCCCTGCTCGAGATCGTCGATCAGCCCGACGTGCGTCGGCTTCCAGGCGAACGCCTGTCGCGTGGAATCGCTGGAGGC
GGGCACATCGAGAGCGAACA

Product: alpha/beta hydrolase domain-containing protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MPFQFSVDEGAEVIRRKLRDLPRREVHPEVEAQDRTIPGPAGDIPIRVYRPATAESSPPVVVFFHGGGFAAGDLDTHDGE
CRHHAVAADAVVVAVDYRLAPEHPYPAAVEDAWAALTWVAGHGAELSVDTNRLAVAGDSAGGNLSAVMTHRARTRGGPPI
AFQLLWYPVTMWDMSLPSVTENAGAPILDRTAMDGLSRWYAGELTDPGHELAPGLADDFTGLPPAYIAVAGHDPLRDDGI
RYAELLSAAGVPVEVHNAETLVHGYLGYAGVVPSATAATDRGLAALRAALHPEAGQRPEK

Sequences:

>Translated_300_residues
MPFQFSVDEGAEVIRRKLRDLPRREVHPEVEAQDRTIPGPAGDIPIRVYRPATAESSPPVVVFFHGGGFAAGDLDTHDGE
CRHHAVAADAVVVAVDYRLAPEHPYPAAVEDAWAALTWVAGHGAELSVDTNRLAVAGDSAGGNLSAVMTHRARTRGGPPI
AFQLLWYPVTMWDMSLPSVTENAGAPILDRTAMDGLSRWYAGELTDPGHELAPGLADDFTGLPPAYIAVAGHDPLRDDGI
RYAELLSAAGVPVEVHNAETLVHGYLGYAGVVPSATAATDRGLAALRAALHPEAGQRPEK
>Mature_299_residues
PFQFSVDEGAEVIRRKLRDLPRREVHPEVEAQDRTIPGPAGDIPIRVYRPATAESSPPVVVFFHGGGFAAGDLDTHDGEC
RHHAVAADAVVVAVDYRLAPEHPYPAAVEDAWAALTWVAGHGAELSVDTNRLAVAGDSAGGNLSAVMTHRARTRGGPPIA
FQLLWYPVTMWDMSLPSVTENAGAPILDRTAMDGLSRWYAGELTDPGHELAPGLADDFTGLPPAYIAVAGHDPLRDDGIR
YAELLSAAGVPVEVHNAETLVHGYLGYAGVVPSATAATDRGLAALRAALHPEAGQRPEK

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=290, Percent_Identity=31.3793103448276, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI157041239, Length=330, Percent_Identity=26.969696969697, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI68299767, Length=315, Percent_Identity=27.9365079365079, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI61966717, Length=194, Percent_Identity=32.4742268041237, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI21328446, Length=158, Percent_Identity=34.8101265822785, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI68051721, Length=161, Percent_Identity=32.2981366459627, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI226423947, Length=169, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786682, Length=286, Percent_Identity=28.6713286713287, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17567059, Length=268, Percent_Identity=29.1044776119403, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540028, Length=185, Percent_Identity=32.4324324324324, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71996133, Length=182, Percent_Identity=30.2197802197802, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI72001146, Length=176, Percent_Identity=31.25, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533410, Length=96, Percent_Identity=35.4166666666667, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=96, Percent_Identity=35.4166666666667, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24656084, Length=89, Percent_Identity=41.5730337078652, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24656076, Length=89, Percent_Identity=41.5730337078652, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20130169, Length=89, Percent_Identity=41.5730337078652, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 31828; Mature: 31697

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS01173 LIPASE_GDXG_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFQFSVDEGAEVIRRKLRDLPRREVHPEVEAQDRTIPGPAGDIPIRVYRPATAESSPPV
CCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCE
VVFFHGGGFAAGDLDTHDGECRHHAVAADAVVVAVDYRLAPEHPYPAAVEDAWAALTWVA
EEEECCCCEEECCCCCCCCCHHHHHEECCEEEEEEEEEECCCCCCCCHHHHHHHEEEEEE
GHGAELSVDTNRLAVAGDSAGGNLSAVMTHRARTRGGPPIAFQLLWYPVTMWDMSLPSVT
CCCCEEEECCCEEEEEECCCCCCCCEEEHHHHHHCCCCCEEEEEEEEEEEEECCCCCCCC
ENAGAPILDRTAMDGLSRWYAGELTDPGHELAPGLADDFTGLPPAYIAVAGHDPLRDDGI
CCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHCCCCCCEEEEEECCCCCCCCCC
RYAELLSAAGVPVEVHNAETLVHGYLGYAGVVPSATAATDRGLAALRAALHPEAGQRPEK
HHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PFQFSVDEGAEVIRRKLRDLPRREVHPEVEAQDRTIPGPAGDIPIRVYRPATAESSPPV
CCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCE
VVFFHGGGFAAGDLDTHDGECRHHAVAADAVVVAVDYRLAPEHPYPAAVEDAWAALTWVA
EEEECCCCEEECCCCCCCCCHHHHHEECCEEEEEEEEEECCCCCCCCHHHHHHHEEEEEE
GHGAELSVDTNRLAVAGDSAGGNLSAVMTHRARTRGGPPIAFQLLWYPVTMWDMSLPSVT
CCCCEEEECCCEEEEEECCCCCCCCEEEHHHHHHCCCCCEEEEEEEEEEEEECCCCCCCC
ENAGAPILDRTAMDGLSRWYAGELTDPGHELAPGLADDFTGLPPAYIAVAGHDPLRDDGI
CCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHCCCCCCEEEEEECCCCCCCCCC
RYAELLSAAGVPVEVHNAETLVHGYLGYAGVVPSATAATDRGLAALRAALHPEAGQRPEK
HHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]