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The map label for this gene is ogt [H]

Identifier: 119868305

GI number: 119868305

Start: 2381959

End: 2382525

Strand: Reverse

Name: ogt [H]

Synonym: Mkms_2269

Alternate gene names: 119868305

Gene position: 2382525-2381959 (Counterclockwise)

Preceding gene: 119868306

Following gene: 119868304

Centisome position: 41.53

GC content: 73.54

Gene sequence:

>567_bases
ATGACCGACGACACCACATTGGCCGGCCTGCACGCGCGGTTGAGCGCCCGAGCCGACGCCGAGGGACTCCTCGACGTCGC
CTACCGGACCGTCGACACCCCCGTCGGCACGCTGCTGCTGGCCGCCACCCCCGCGGGTCTGGTGCGGGTGGCCTATGACA
TCGAGGACCACACCGCGGTGCTGACCACGCTGGCCACCAGGATCAGCCCGCGCATCCTGCGGGCGCCGCACCGGCTCGAC
ACCGCGGCGCGTCAGCTCGACGAATACTTCGCCAAGCGGCGCACCGCCTTCGACGTGCCGATCGACCTGCGGCTCGCCGA
GGGATTCCGCCGCGACGTCGTCGAACAGTTGCGCACCATCGGCTACGGCCAGCGCGCCAGCTACGCCGCGGTGGCCGCCG
CGGTCGGCAACCCCAGGGCGGTGCGGGCCGTCGGGAGCGCCTGCGCGCACAACCCGCTGCCGGTGGTGATCCCCTGCCAC
CGGGTGGTGCGCTCCGACGGCACGGCCGGCCAGTACGTCGGCGGATCGGCCGCCAAGGCGGCACTGCTGCGGCTCGAGCG
GGAATGA

Upstream 100 bases:

>100_bases
AGATCGCGGGACTGCTCGGCAACTCCCCCGACGCCGCCCGCCGCGCGTGTGCCGACGGCTTGAGCAGACTGCGCTCGATC
TACCGCGAGGAGCCCCTCCC

Downstream 100 bases:

>100_bases
AACACCCACCTCCGCGGTGGAACTGCACACACGCGACTCCACGGGCCCCAGGGTCTGAACCGGTGTGGCGTCTCGTCGGG
GTGTGCCATCATTCGAGGCA

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MTDDTTLAGLHARLSARADAEGLLDVAYRTVDTPVGTLLLAATPAGLVRVAYDIEDHTAVLTTLATRISPRILRAPHRLD
TAARQLDEYFAKRRTAFDVPIDLRLAEGFRRDVVEQLRTIGYGQRASYAAVAAAVGNPRAVRAVGSACAHNPLPVVIPCH
RVVRSDGTAGQYVGGSAAKAALLRLERE

Sequences:

>Translated_188_residues
MTDDTTLAGLHARLSARADAEGLLDVAYRTVDTPVGTLLLAATPAGLVRVAYDIEDHTAVLTTLATRISPRILRAPHRLD
TAARQLDEYFAKRRTAFDVPIDLRLAEGFRRDVVEQLRTIGYGQRASYAAVAAAVGNPRAVRAVGSACAHNPLPVVIPCH
RVVRSDGTAGQYVGGSAAKAALLRLERE
>Mature_187_residues
TDDTTLAGLHARLSARADAEGLLDVAYRTVDTPVGTLLLAATPAGLVRVAYDIEDHTAVLTTLATRISPRILRAPHRLDT
AARQLDEYFAKRRTAFDVPIDLRLAEGFRRDVVEQLRTIGYGQRASYAAVAAAVGNPRAVRAVGSACAHNPLPVVIPCHR
VVRSDGTAGQYVGGSAAKAALLRLERE

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=163, Percent_Identity=34.3558282208589, Blast_Score=91, Evalue=7e-19,
Organism=Escherichia coli, GI1788542, Length=167, Percent_Identity=38.9221556886228, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=42.156862745098, Blast_Score=79, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=78, Percent_Identity=44.8717948717949, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115533068, Length=78, Percent_Identity=44.8717948717949, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320001, Length=90, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20135; Mature: 20004

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDDTTLAGLHARLSARADAEGLLDVAYRTVDTPVGTLLLAATPAGLVRVAYDIEDHTAV
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCHHHH
LTTLATRISPRILRAPHRLDTAARQLDEYFAKRRTAFDVPIDLRLAEGFRRDVVEQLRTI
HHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHC
GYGQRASYAAVAAAVGNPRAVRAVGSACAHNPLPVVIPCHRVVRSDGTAGQYVGGSAAKA
CCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECHHHHHCCCCCCCCCCCHHHHH
ALLRLERE
HHHHHCCC
>Mature Secondary Structure 
TDDTTLAGLHARLSARADAEGLLDVAYRTVDTPVGTLLLAATPAGLVRVAYDIEDHTAV
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCHHHH
LTTLATRISPRILRAPHRLDTAARQLDEYFAKRRTAFDVPIDLRLAEGFRRDVVEQLRTI
HHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHC
GYGQRASYAAVAAAVGNPRAVRAVGSACAHNPLPVVIPCHRVVRSDGTAGQYVGGSAAKA
CCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECHHHHHCCCCCCCCCCCHHHHH
ALLRLERE
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA