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The map label for this gene is rpoD [H]

Identifier: 119868267

GI number: 119868267

Start: 2343928

End: 2345370

Strand: Reverse

Name: rpoD [H]

Synonym: Mkms_2231

Alternate gene names: 119868267

Gene position: 2345370-2343928 (Counterclockwise)

Preceding gene: 119868268

Following gene: 119868266

Centisome position: 40.88

GC content: 68.88

Gene sequence:

>1443_bases
GTGGCAGCGACAAAGGCAAACCCGGCAACCGAAGAGCCGGTGAAGCGCACCGCTACCAAGACCCCCGCGAAGAAGACCGC
CGCGGCGAAGGCCCCAGCCAAACGCGCGGCCAAGGGTACGACGACGACCCGCGGCCCGGCCAAGAAGGACGGCGCCGCCC
CGCGCGGTCGTGGCAAGAAGTCCACCGCACCCGAGGCCGGTGCCGCCGACGCCCTCGCCGACGACGACCTCGACACCGAC
GACACCCTCGAGGCCGAACCGGATATCGACGTCGACGACGCCGACCTGGACCTCGAGGATCTCGACACCGACGACGACTC
GTCCGACGACGGCGACGACGCCGACACCCCCGACGCCAAGGCCAAGGCCGCCCCCAAGGGCGGCGCGGTGCCCGCCGCCC
CCGCCACCGAGGACGAGGAGATCGCCGAGCCCTCCGAGAAGGACAAGGCCTCCGGCGACTTCGTCTGGGACGAGGAGGAG
TCCGAGGCGCTGCGGCAGGCCCGCAAGGACGCCGAGCTCACCGCCTCCGCCGACTCGGTGCGCGCGTATCTCAAGCAGAT
CGGCAAGGTGGCGCTGCTCAACGCCGAGGAGGAAGTCGAGCTCGCCAAGCGCATCGAGGCCGGTCTGTTCGCCACCCAGA
AGCTGGCCGAACTCGCCGAAAAGGGTGAGAAGCTGCCGGTGCAGCAGCGCCGCGACATGCAGTGGATCTGCCGCGACGGC
GACCGCGCCAAGAACCACCTGCTGGAGGCGAACCTCCGCCTGGTGGTGTCGCTGGCCAAGCGCTACACCGGCCGTGGCAT
GGCGTTTCTGGACCTCATCCAGGAGGGCAACCTCGGTCTGATCCGCGCGGTCGAGAAGTTCGACTACACCAAGGGTTACA
AGTTCTCCACCTACGCCACCTGGTGGATCCGGCAGGCGATCACCCGCGCGATGGCCGACCAGGCGCGCACCATCCGCATC
CCGGTGCACATGGTCGAGGTCATCAACAAGCTGGGCCGCATCCAGCGCGAGCTGCTCCAGGACCTGGGTCGCGAACCCAC
GCCCGAAGAGCTCGCCAAGGAGATGGACATCACGCCGGAGAAGGTGCTGGAGATCCAGCAGTACGCGCGTGAGCCGATCT
CGCTGGACCAGACGATCGGCGACGAGGGCGACAGCCAGCTCGGCGACTTCATCGAGGACTCCGAGGCCGTGGTGGCCGTG
GACGCGGTCTCGTTCACGCTGCTGCAGGATCAGCTGCAGTCGGTGCTGGAGACGCTGTCGGAGCGCGAGGCCGGCGTGGT
ACGGCTGCGGTTCGGCCTCACCGACGGCCAGCCGCGCACGCTCGACGAGATCGGCCAGGTCTACGGCGTCACGCGGGAAC
GCATCCGCCAGATCGAGTCGAAGACGATGAGCAAGCTCCGGCACCCCAGCCGGTCGCAGGTGCTGCGCGACTACCTCGAC
TGA

Upstream 100 bases:

>100_bases
CCGACATTCGACATAGCCGACATTTCGGGTTGGCGCCTGCTCCCCAACATCCGTTGGGGTCTCGGGTGCCCACCGGAACA
GTAAGACCGAAAGGGTGTAC

Downstream 100 bases:

>100_bases
CCCGTGCCGAAGCCTCCGAGAGTTCCGCCTCAGCGGGTGGTGCGCGCAGTCGACCGTGTCCGTGCCGGTCTGCAGCGCCT
GCACCGCACGACCGCACCGG

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MAATKANPATEEPVKRTATKTPAKKTAAAKAPAKRAAKGTTTTRGPAKKDGAAPRGRGKKSTAPEAGAADALADDDLDTD
DTLEAEPDIDVDDADLDLEDLDTDDDSSDDGDDADTPDAKAKAAPKGGAVPAAPATEDEEIAEPSEKDKASGDFVWDEEE
SEALRQARKDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLFATQKLAELAEKGEKLPVQQRRDMQWICRDG
DRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRI
PVHMVEVINKLGRIQRELLQDLGREPTPEELAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAV
DAVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD

Sequences:

>Translated_480_residues
MAATKANPATEEPVKRTATKTPAKKTAAAKAPAKRAAKGTTTTRGPAKKDGAAPRGRGKKSTAPEAGAADALADDDLDTD
DTLEAEPDIDVDDADLDLEDLDTDDDSSDDGDDADTPDAKAKAAPKGGAVPAAPATEDEEIAEPSEKDKASGDFVWDEEE
SEALRQARKDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLFATQKLAELAEKGEKLPVQQRRDMQWICRDG
DRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRI
PVHMVEVINKLGRIQRELLQDLGREPTPEELAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAV
DAVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD
>Mature_479_residues
AATKANPATEEPVKRTATKTPAKKTAAAKAPAKRAAKGTTTTRGPAKKDGAAPRGRGKKSTAPEAGAADALADDDLDTDD
TLEAEPDIDVDDADLDLEDLDTDDDSSDDGDDADTPDAKAKAAPKGGAVPAAPATEDEEIAEPSEKDKASGDFVWDEEES
EALRQARKDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLFATQKLAELAEKGEKLPVQQRRDMQWICRDGD
RAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIP
VHMVEVINKLGRIQRELLQDLGREPTPEELAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVD
AVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=274, Percent_Identity=55.8394160583942, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1789098, Length=298, Percent_Identity=38.9261744966443, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1789871, Length=231, Percent_Identity=30.7359307359307, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788231, Length=204, Percent_Identity=28.921568627451, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 52726; Mature: 52595

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATKANPATEEPVKRTATKTPAKKTAAAKAPAKRAAKGTTTTRGPAKKDGAAPRGRGKK
CCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
STAPEAGAADALADDDLDTDDTLEAEPDIDVDDADLDLEDLDTDDDSSDDGDDADTPDAK
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCC
AKAAPKGGAVPAAPATEDEEIAEPSEKDKASGDFVWDEEESEALRQARKDAELTASADSV
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHH
RAYLKQIGKVALLNAEEEVELAKRIEAGLFATQKLAELAEKGEKLPVQQRRDMQWICRDG
HHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHCCC
DRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYAT
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHH
WWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPEELAKEMDITPE
HHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHH
KVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFTLLQDQLQSVLETLS
HHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
EREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD
HHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure 
AATKANPATEEPVKRTATKTPAKKTAAAKAPAKRAAKGTTTTRGPAKKDGAAPRGRGKK
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
STAPEAGAADALADDDLDTDDTLEAEPDIDVDDADLDLEDLDTDDDSSDDGDDADTPDAK
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCC
AKAAPKGGAVPAAPATEDEEIAEPSEKDKASGDFVWDEEESEALRQARKDAELTASADSV
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHH
RAYLKQIGKVALLNAEEEVELAKRIEAGLFATQKLAELAEKGEKLPVQQRRDMQWICRDG
HHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHCCC
DRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYAT
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHH
WWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPEELAKEMDITPE
HHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHH
KVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFTLLQDQLQSVLETLS
HHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
EREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD
HHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]