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The map label for this gene is cocE [H]

Identifier: 119868173

GI number: 119868173

Start: 2259842

End: 2261497

Strand: Reverse

Name: cocE [H]

Synonym: Mkms_2137

Alternate gene names: 119868173

Gene position: 2261497-2259842 (Counterclockwise)

Preceding gene: 119868174

Following gene: 119868170

Centisome position: 39.42

GC content: 71.8

Gene sequence:

>1656_bases
ATGACGTCAACCGGTCCCGCGGTATTGCGCCGTTCCGTCGGCAACGCAATCAGCCGGCTGCTCTCCGTCCCCTCGCACAC
CAGTGGTTTCGCCGTCGAGCGCGGGGTGCGGGTGCCGATGCGCGACGGTGTCGACCTGATCGCCGACCACTACATCCCGG
ACACCCCGACCGCGTCGGGCACGTTGCTGGTGCGCGGACCGTACGGTCGCGGCTGGCCGTTCTCCCTGCTGTTCGCGGTC
GTCTACGCGACGCGCGGGTACCACGTGGTGGTGCAGAGCGTGCGAGGCACATTCGGCTCCGGTGGCGAGTTCACCCCGAT
GGTCCACGAGAAGGACGACGGCGCCGACACGGCGGCCTGGCTGCGTGAACAACCCTGGTTCACCGGGTCTTTCGCCACCG
TCGGGCTGTCCTACCTCGGGTTCACCCAGTGGGCGCTGCTCACCGACCCCCCGCCCGAACTCAGGGCCGCCGTGATCGCG
GTCGGACCGCACGACTTCCGCGCGTCGTCGTGGGGCACCGGGTCGTTCTCGCTCAACGACTTCCTGGGCTGGAGCGACAT
GGTCGCCCGTCAGGAGGACACCCGGGGAATCGCGGCCCTGATCCGGCAGGTGCGGGCGCGCCGGGCACTGGCCCGCGCGA
CCGCCGACCTTCCGCTCGGTGCGGCCGGACGCCGACTCCTCGGCGACGGGGCGCACTGGTACGAGTCGTGGATCGAACAG
GCCGACGACGCCGCGTTCTGGGACGAGTTGCGCGCCAACGAGGCGCTCGACCGCGTCGACATCCCGATCCTGCTGTTCGG
CGGCTGGCAGGACCTGTTCCTCGAGCAGACCCTGCACCAGTACACCCATCTGCGGGGCCGGGGTGTGCCCGCCGCGCTGA
CCGTCGGGGCGTGGACGCATACGCAGCTGATGACGAAAGGCGCCCCCACGGTGGTGCGTGAGACGCTGGACTGGCTGGGC
ACCCACCTGGGCGGCGGCCCGGAATCCGGGCGCGCGCCGGTGCGCATCGAGATCAACAACGACGGGTGGCACGACCTCGC
GGACTGGCCGCCGCCGATGCCCGAGTACGTGCTGTTCCTGCGCACCACCGGCCAATTGTCCGCGGCGCCACCGGATCCGA
CCGCACCGCCGTCGGTGTTCACCTTCGATCCGACCGACCCCACCCCGACCGTCGGCGGTCGGCTGCTGTCCCCGCAGGGC
GGCTACCGCGCCGACCGGCGGCTGGCCGAACGCGATGACGTGCTCGCCTTCACCGGCGACCCGCTGCCCGCGGACCTCTA
CGTGGTGGGAACGCCCGTGGTCGAACTCGCGCACTCGTGCGACAACCCGCACCGCGATCTGTTCGTCCGCCTGTCCGAGG
TGGACCGGAAAGGCCGCTCCCGCAACGTCACCGACGGGTTCCGGCGCCTGACGGGTGAGGACGGCACCGTGCGGCTCGAA
CTCGACGCGGTGGCCCACCGCTTCCGCGCGGGCTCGCGCCTGCGAGTCCTGGTCGCCGGCGGCTCGCATCCGCGGTTCGC
CCGCAATCTCGGGACAGGTGAACCGCCGATCAGCGGGAGTACGACGGTCCCGGCCACCCACACGGTGCACCACGGGCGGG
GCGGGGTGTCACGTCTGCTCCTGCCCGCGGGCCCGCAGCCCCCGTCAGCCCACTGA

Upstream 100 bases:

>100_bases
TGCAGACCAACCTGCAGAAGGCGCACGAACTGGCGCCGCACTACATCTACTGACACATCTACCGAAGGGCGGCCGGGGTC
CGCTGACTACCCTTGGCCGG

Downstream 100 bases:

>100_bases
GCGGCGCACCGACTCGGCGATCGCGGCGAGCGTCGCGTCGTCGTGCACGGGCGGCTCCCACGCCGGGTGCACCGGCAGGT
CCACCCAGCTGGTGCAGCCG

Product: peptidase S15

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MTSTGPAVLRRSVGNAISRLLSVPSHTSGFAVERGVRVPMRDGVDLIADHYIPDTPTASGTLLVRGPYGRGWPFSLLFAV
VYATRGYHVVVQSVRGTFGSGGEFTPMVHEKDDGADTAAWLREQPWFTGSFATVGLSYLGFTQWALLTDPPPELRAAVIA
VGPHDFRASSWGTGSFSLNDFLGWSDMVARQEDTRGIAALIRQVRARRALARATADLPLGAAGRRLLGDGAHWYESWIEQ
ADDAAFWDELRANEALDRVDIPILLFGGWQDLFLEQTLHQYTHLRGRGVPAALTVGAWTHTQLMTKGAPTVVRETLDWLG
THLGGGPESGRAPVRIEINNDGWHDLADWPPPMPEYVLFLRTTGQLSAAPPDPTAPPSVFTFDPTDPTPTVGGRLLSPQG
GYRADRRLAERDDVLAFTGDPLPADLYVVGTPVVELAHSCDNPHRDLFVRLSEVDRKGRSRNVTDGFRRLTGEDGTVRLE
LDAVAHRFRAGSRLRVLVAGGSHPRFARNLGTGEPPISGSTTVPATHTVHHGRGGVSRLLLPAGPQPPSAH

Sequences:

>Translated_551_residues
MTSTGPAVLRRSVGNAISRLLSVPSHTSGFAVERGVRVPMRDGVDLIADHYIPDTPTASGTLLVRGPYGRGWPFSLLFAV
VYATRGYHVVVQSVRGTFGSGGEFTPMVHEKDDGADTAAWLREQPWFTGSFATVGLSYLGFTQWALLTDPPPELRAAVIA
VGPHDFRASSWGTGSFSLNDFLGWSDMVARQEDTRGIAALIRQVRARRALARATADLPLGAAGRRLLGDGAHWYESWIEQ
ADDAAFWDELRANEALDRVDIPILLFGGWQDLFLEQTLHQYTHLRGRGVPAALTVGAWTHTQLMTKGAPTVVRETLDWLG
THLGGGPESGRAPVRIEINNDGWHDLADWPPPMPEYVLFLRTTGQLSAAPPDPTAPPSVFTFDPTDPTPTVGGRLLSPQG
GYRADRRLAERDDVLAFTGDPLPADLYVVGTPVVELAHSCDNPHRDLFVRLSEVDRKGRSRNVTDGFRRLTGEDGTVRLE
LDAVAHRFRAGSRLRVLVAGGSHPRFARNLGTGEPPISGSTTVPATHTVHHGRGGVSRLLLPAGPQPPSAH
>Mature_550_residues
TSTGPAVLRRSVGNAISRLLSVPSHTSGFAVERGVRVPMRDGVDLIADHYIPDTPTASGTLLVRGPYGRGWPFSLLFAVV
YATRGYHVVVQSVRGTFGSGGEFTPMVHEKDDGADTAAWLREQPWFTGSFATVGLSYLGFTQWALLTDPPPELRAAVIAV
GPHDFRASSWGTGSFSLNDFLGWSDMVARQEDTRGIAALIRQVRARRALARATADLPLGAAGRRLLGDGAHWYESWIEQA
DDAAFWDELRANEALDRVDIPILLFGGWQDLFLEQTLHQYTHLRGRGVPAALTVGAWTHTQLMTKGAPTVVRETLDWLGT
HLGGGPESGRAPVRIEINNDGWHDLADWPPPMPEYVLFLRTTGQLSAAPPDPTAPPSVFTFDPTDPTPTVGGRLLSPQGG
YRADRRLAERDDVLAFTGDPLPADLYVVGTPVVELAHSCDNPHRDLFVRLSEVDRKGRSRNVTDGFRRLTGEDGTVRLEL
DAVAHRFRAGSRLRVLVAGGSHPRFARNLGTGEPPISGSTTVPATHTVHHGRGGVSRLLLPAGPQPPSAH

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 59756; Mature: 59625

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTGPAVLRRSVGNAISRLLSVPSHTSGFAVERGVRVPMRDGVDLIADHYIPDTPTASG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCCCCCCCCC
TLLVRGPYGRGWPFSLLFAVVYATRGYHVVVQSVRGTFGSGGEFTPMVHEKDDGADTAAW
EEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCCEECCCCCCHHHHH
LREQPWFTGSFATVGLSYLGFTQWALLTDPPPELRAAVIAVGPHDFRASSWGTGSFSLND
HHCCCCCCCCHHHHHHHHHHHHEEEEECCCCHHHEEEEEEECCCCCCCCCCCCCCCCHHH
FLGWSDMVARQEDTRGIAALIRQVRARRALARATADLPLGAAGRRLLGDGAHWYESWIEQ
HCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHH
ADDAAFWDELRANEALDRVDIPILLFGGWQDLFLEQTLHQYTHLRGRGVPAALTVGAWTH
CCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHH
TQLMTKGAPTVVRETLDWLGTHLGGGPESGRAPVRIEINNDGWHDLADWPPPMPEYVLFL
HHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHEEEEE
RTTGQLSAAPPDPTAPPSVFTFDPTDPTPTVGGRLLSPQGGYRADRRLAERDDVLAFTGD
EECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEECCCCCCHHHHHHHCCCCEEEEECC
PLPADLYVVGTPVVELAHSCDNPHRDLFVRLSEVDRKGRSRNVTDGFRRLTGEDGTVRLE
CCCCCEEEECCHHHHHHHHCCCCCHHHEEEHHHHHCCCCCCCHHHHHHHHCCCCCEEEEE
LDAVAHRFRAGSRLRVLVAGGSHPRFARNLGTGEPPISGSTTVPATHTVHHGRGGVSRLL
HHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCEEE
LPAGPQPPSAH
ECCCCCCCCCC
>Mature Secondary Structure 
TSTGPAVLRRSVGNAISRLLSVPSHTSGFAVERGVRVPMRDGVDLIADHYIPDTPTASG
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCCCCCCCCC
TLLVRGPYGRGWPFSLLFAVVYATRGYHVVVQSVRGTFGSGGEFTPMVHEKDDGADTAAW
EEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCCEECCCCCCHHHHH
LREQPWFTGSFATVGLSYLGFTQWALLTDPPPELRAAVIAVGPHDFRASSWGTGSFSLND
HHCCCCCCCCHHHHHHHHHHHHEEEEECCCCHHHEEEEEEECCCCCCCCCCCCCCCCHHH
FLGWSDMVARQEDTRGIAALIRQVRARRALARATADLPLGAAGRRLLGDGAHWYESWIEQ
HCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHH
ADDAAFWDELRANEALDRVDIPILLFGGWQDLFLEQTLHQYTHLRGRGVPAALTVGAWTH
CCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHH
TQLMTKGAPTVVRETLDWLGTHLGGGPESGRAPVRIEINNDGWHDLADWPPPMPEYVLFL
HHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHEEEEE
RTTGQLSAAPPDPTAPPSVFTFDPTDPTPTVGGRLLSPQGGYRADRRLAERDDVLAFTGD
EECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEECCCCCCHHHHHHHCCCCEEEEECC
PLPADLYVVGTPVVELAHSCDNPHRDLFVRLSEVDRKGRSRNVTDGFRRLTGEDGTVRLE
CCCCCEEEECCHHHHHHHHCCCCCHHHEEEHHHHHCCCCCCCHHHHHHHHCCCCCEEEEE
LDAVAHRFRAGSRLRVLVAGGSHPRFARNLGTGEPPISGSTTVPATHTVHHGRGGVSRLL
HHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCEEE
LPAGPQPPSAH
ECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]