BacMap

Definition	Mycobacterium sp. KMS chromosome, complete genome.
Accession	NC_008705
Length	5,737,227

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The map label for this gene is hetN [H]

Identifier: 119867985

GI number: 119867985

Start: 2065392

End: 2066180

Strand: Reverse

Name: hetN [H]

Synonym: Mkms_1948

Alternate gene names: 119867985

Gene position: 2066180-2065392 (Counterclockwise)

Preceding gene: 119867986

Following gene: 119867984

Centisome position: 36.01

GC content: 70.98

Gene sequence:

>789_bases
GTGACGGGACGTTGCACGGGCAAGGTGGCGCTGGTGACCGGGAGCAGCCGGGGCCTGGGTGCGGCGATCGCGACACGGCT
GGCCGCCGAGGGGGCGGCGGTGGTGCTCACGGCGCGCACCCTGGAACCGGATCCGAGATACCGGGGGTCGCTGCAGGACA
CGCGCGACACGATCCTCACCGCGGGCGGCACCGCGGTCGCCGTCGCCGCGGACCTTTCCCGGCCCGAGGAGCGCGAGCGG
CTGTTCGCGGAGGCGACGAAGGAACTCGGCGCACCGGACATCCTCGTCAACAACGCGGCGGTGACGTTCCTGCGACCGCT
CGACGGTTTTCCCGACAAGCGGGTGCGCTTGATGCTGGAGATGCACCTGCTCGCACCACTGCACCTGAGCCAGTTGGCGA
TCCCCGCGATGCGCGAACGCGGCCGCGGCTGGATCCTCAACGTGACGTCGGTGGCCGGCGACCTGCCGCCCGGTCCGCCG
TTCTCCGAGTTCGACCGCACCGCCGGGTTCGGCGTCTACGGCACGGCGAAGGCCGCGCTGAACCGATTGACGAAAAGCCT
TGCCGCAGAACTGTTCGACGACGGGATCGCGGTCAACGCGGCGGCGCCGACGAACCCGGTCGCCACGCCCGGCGCCGGCA
CGCTCGACCTGGCCAAGACCGACACCGAGGACATCAGCCTCATCGCCGAGACGGCGCTCACGCTGTGCACCGGTGATCCG
GCGACGCTGACCGGACGGATCGCCCACACCCAGTCGTTCCTCGGCGAGATCGGTTGGCTGCGTGAGTGA

Upstream 100 bases:

>100_bases
GGGCCTCGACCACACATCTGGCCAAGTCACCCTCGGCGGAAACCCCACGCCCGTAACGTGCTTCTCGTCTCACGAGAAGC
ACATTGCCGGGAGGTGGTCG

Downstream 100 bases:

>100_bases
ACTCGACCTCGAATCGCTGCGGACGCGGCTGGCGGCGGCCGGCGTCTGCGATGTTCGTCCGCTCACCGGCGGGGCGTCGA
GCCTGACCTTCCGCGCCACC

Product: short-chain dehydrogenase/reductase SDR

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTGRCTGKVALVTGSSRGLGAAIATRLAAEGAAVVLTARTLEPDPRYRGSLQDTRDTILTAGGTAVAVAADLSRPEERER
LFAEATKELGAPDILVNNAAVTFLRPLDGFPDKRVRLMLEMHLLAPLHLSQLAIPAMRERGRGWILNVTSVAGDLPPGPP
FSEFDRTAGFGVYGTAKAALNRLTKSLAAELFDDGIAVNAAAPTNPVATPGAGTLDLAKTDTEDISLIAETALTLCTGDP
ATLTGRIAHTQSFLGEIGWLRE

Sequences:

>Translated_262_residues
MTGRCTGKVALVTGSSRGLGAAIATRLAAEGAAVVLTARTLEPDPRYRGSLQDTRDTILTAGGTAVAVAADLSRPEERER
LFAEATKELGAPDILVNNAAVTFLRPLDGFPDKRVRLMLEMHLLAPLHLSQLAIPAMRERGRGWILNVTSVAGDLPPGPP
FSEFDRTAGFGVYGTAKAALNRLTKSLAAELFDDGIAVNAAAPTNPVATPGAGTLDLAKTDTEDISLIAETALTLCTGDP
ATLTGRIAHTQSFLGEIGWLRE
>Mature_261_residues
TGRCTGKVALVTGSSRGLGAAIATRLAAEGAAVVLTARTLEPDPRYRGSLQDTRDTILTAGGTAVAVAADLSRPEERERL
FAEATKELGAPDILVNNAAVTFLRPLDGFPDKRVRLMLEMHLLAPLHLSQLAIPAMRERGRGWILNVTSVAGDLPPGPPF
SEFDRTAGFGVYGTAKAALNRLTKSLAAELFDDGIAVNAAAPTNPVATPGAGTLDLAKTDTEDISLIAETALTLCTGDPA
TLTGRIAHTQSFLGEIGWLRE

Specific function: May be involved in repressing heterocyst differentiation and may be essential for preventing all vegetative cells from differentiating [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254986, Length=264, Percent_Identity=29.1666666666667, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI32483357, Length=197, Percent_Identity=38.0710659898477, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI126723191, Length=196, Percent_Identity=36.734693877551, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI33667109, Length=198, Percent_Identity=33.8383838383838, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI5031737, Length=198, Percent_Identity=33.8383838383838, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI19923817, Length=206, Percent_Identity=29.6116504854369, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI7705925, Length=209, Percent_Identity=31.5789473684211, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI31542939, Length=205, Percent_Identity=28.2926829268293, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI40254992, Length=196, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI210032110, Length=155, Percent_Identity=29.6774193548387, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787335, Length=197, Percent_Identity=34.5177664974619, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082100, Length=213, Percent_Identity=32.3943661971831, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787905, Length=254, Percent_Identity=31.1023622047244, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790717, Length=246, Percent_Identity=28.0487804878049, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI2367365, Length=208, Percent_Identity=33.1730769230769, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788459, Length=210, Percent_Identity=31.4285714285714, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367175, Length=202, Percent_Identity=30.6930693069307, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082160, Length=197, Percent_Identity=28.9340101522843, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787820, Length=196, Percent_Identity=26.530612244898, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789378, Length=244, Percent_Identity=28.2786885245902, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17562910, Length=236, Percent_Identity=32.2033898305085, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17562908, Length=210, Percent_Identity=32.3809523809524, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17560332, Length=214, Percent_Identity=31.7757009345794, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562906, Length=207, Percent_Identity=32.8502415458937, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71982365, Length=255, Percent_Identity=26.2745098039216, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17531453, Length=244, Percent_Identity=31.9672131147541, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI193204405, Length=207, Percent_Identity=33.8164251207729, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17560220, Length=207, Percent_Identity=32.3671497584541, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17538486, Length=210, Percent_Identity=31.9047619047619, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559104, Length=214, Percent_Identity=30.8411214953271, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538480, Length=211, Percent_Identity=33.175355450237, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17562904, Length=211, Percent_Identity=32.2274881516588, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17561402, Length=265, Percent_Identity=28.3018867924528, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17558628, Length=249, Percent_Identity=27.3092369477912, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17551412, Length=217, Percent_Identity=31.3364055299539, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560150, Length=230, Percent_Identity=30.4347826086957, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17565030, Length=211, Percent_Identity=31.7535545023697, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560676, Length=203, Percent_Identity=33.4975369458128, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562990, Length=227, Percent_Identity=31.7180616740088, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI86565303, Length=211, Percent_Identity=31.7535545023697, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=208, Percent_Identity=29.3269230769231, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17555612, Length=213, Percent_Identity=31.924882629108, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71997099, Length=221, Percent_Identity=32.1266968325792, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17564282, Length=147, Percent_Identity=29.2517006802721, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17536025, Length=213, Percent_Identity=30.9859154929577, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=208, Percent_Identity=27.4038461538462, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17561272, Length=213, Percent_Identity=32.8638497652582, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17536651, Length=212, Percent_Identity=30.188679245283, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17568967, Length=212, Percent_Identity=31.1320754716981, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=198, Percent_Identity=31.8181818181818, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357673, Length=251, Percent_Identity=30.6772908366534, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21355319, Length=205, Percent_Identity=33.6585365853659, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24644339, Length=199, Percent_Identity=27.6381909547739, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28571526, Length=197, Percent_Identity=30.9644670050761, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI23397609, Length=198, Percent_Identity=30.3030303030303, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357041, Length=197, Percent_Identity=28.4263959390863, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24640492, Length=148, Percent_Identity=30.4054054054054, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24640442, Length=203, Percent_Identity=30.0492610837438, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21355631, Length=186, Percent_Identity=30.1075268817204, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI18859837, Length=203, Percent_Identity=30.0492610837438, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI28571387, Length=205, Percent_Identity=28.780487804878, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007236, Length=205, Percent_Identity=28.2926829268293, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 27453; Mature: 27321

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRCTGKVALVTGSSRGLGAAIATRLAAEGAAVVLTARTLEPDPRYRGSLQDTRDTILT
CCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHEEE
AGGTAVAVAADLSRPEERERLFAEATKELGAPDILVNNAAVTFLRPLDGFPDKRVRLMLE
CCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEECCCEEEEEECCCCCCCHHHHHHHH
MHLLAPLHLSQLAIPAMRERGRGWILNVTSVAGDLPPGPPFSEFDRTAGFGVYGTAKAAL
HHHHCHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCHHHHHHCCCCCCEEHHHHHH
NRLTKSLAAELFDDGIAVNAAAPTNPVATPGAGTLDLAKTDTEDISLIAETALTLCTGDP
HHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCC
ATLTGRIAHTQSFLGEIGWLRE
CCEEEHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TGRCTGKVALVTGSSRGLGAAIATRLAAEGAAVVLTARTLEPDPRYRGSLQDTRDTILT
CCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHEEE
AGGTAVAVAADLSRPEERERLFAEATKELGAPDILVNNAAVTFLRPLDGFPDKRVRLMLE
CCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEECCCEEEEEECCCCCCCHHHHHHHH
MHLLAPLHLSQLAIPAMRERGRGWILNVTSVAGDLPPGPPFSEFDRTAGFGVYGTAKAAL
HHHHCHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCHHHHHHCCCCCCEEHHHHHH
NRLTKSLAAELFDDGIAVNAAAPTNPVATPGAGTLDLAKTDTEDISLIAETALTLCTGDP
HHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCC
ATLTGRIAHTQSFLGEIGWLRE
CCEEEHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8157596; 11759840 [H]