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The map label for this gene is yagI [H]

Identifier: 119867781

GI number: 119867781

Start: 1854650

End: 1855354

Strand: Reverse

Name: yagI [H]

Synonym: Mkms_1741

Alternate gene names: 119867781

Gene position: 1855354-1854650 (Counterclockwise)

Preceding gene: 119867795

Following gene: 119867780

Centisome position: 32.34

GC content: 66.38

Gene sequence:

>705_bases
TTGCTGAACATTCTCGGCGCCTTCGACGAGCGGCATAACAGCCTGACGCTGTCCGAGTTGGCCCGCCGGGCCGGACTGCC
GATTGCCACCACACACCGCCTTGCGGCCGAGTTGGTCGCCGGCTCGGCATTGCAGCGCCGCGCCGACGGTCAGTACGTGG
TGGGCAGGCTGGTCTGGAACGCGGGATTGCTGGCGCCGGTGGAAGGCCAATTGCGCCAGGTGGCTGAACCGTTCTTGTAT
GACGTATACGCGGCGACCCTTGCCACCGTGCACCTGGCCGTGCGCGATGGCGACGAGGTGCTGTACCTGGAACGGATGAT
GGGCCGGACATCTGTCCCGATTGTCAGCGCCGTCGGCAGCAGGCTGCCGATGCACTGCACCGGCGTTGGCAAGGTGCTGC
TGGCCCACGCGCCGGAGGACGTTAAAGACCGAGTGTTCGCCACCTTGACTCGCATCACGCCGTACACCATCGTGTCGCCG
ACCGTGCTGTCTTCGCAGATGCAGCGCATCCATCGTGAAGGAGTGGCCACCACGAGCGAGGAGATGTCGCTGGGTGCATG
TTCGCTGGCGGTGCCGGTCATCCGCCAGACCGACGACGCCGTAGTCGCCGCGATCGCCGTGGTGGTACCGACGCTGAAGC
GCGATCGTCAGCGGCTTCTCGGCGCCCTGCAGGTCGCCGCGCGCGGCATTGGGCGACTGCTATGA

Upstream 100 bases:

>100_bases
CGACGTTGAGATTGGCGAACGGTAGAATTCCACTGAGCGGAAAGGCCATCCATAATTGGCAGGGAACACTTCGACGCCGG
GAACCACTGTCACCTCGCGT

Downstream 100 bases:

>100_bases
CCCGTCAGGCGCCGTCGTAGCCGTCGCTCTGTGGGACAGAATCTCTCGAACGGGCAAGCTTTGGCGGCGGCCGCAACCTA
GGTCGAAGTGCCACACGTTA

Product: regulatory proteins IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MLNILGAFDERHNSLTLSELARRAGLPIATTHRLAAELVAGSALQRRADGQYVVGRLVWNAGLLAPVEGQLRQVAEPFLY
DVYAATLATVHLAVRDGDEVLYLERMMGRTSVPIVSAVGSRLPMHCTGVGKVLLAHAPEDVKDRVFATLTRITPYTIVSP
TVLSSQMQRIHREGVATTSEEMSLGACSLAVPVIRQTDDAVVAAIAVVVPTLKRDRQRLLGALQVAARGIGRLL

Sequences:

>Translated_234_residues
MLNILGAFDERHNSLTLSELARRAGLPIATTHRLAAELVAGSALQRRADGQYVVGRLVWNAGLLAPVEGQLRQVAEPFLY
DVYAATLATVHLAVRDGDEVLYLERMMGRTSVPIVSAVGSRLPMHCTGVGKVLLAHAPEDVKDRVFATLTRITPYTIVSP
TVLSSQMQRIHREGVATTSEEMSLGACSLAVPVIRQTDDAVVAAIAVVVPTLKRDRQRLLGALQVAARGIGRLL
>Mature_234_residues
MLNILGAFDERHNSLTLSELARRAGLPIATTHRLAAELVAGSALQRRADGQYVVGRLVWNAGLLAPVEGQLRQVAEPFLY
DVYAATLATVHLAVRDGDEVLYLERMMGRTSVPIVSAVGSRLPMHCTGVGKVLLAHAPEDVKDRVFATLTRITPYTIVSP
TVLSSQMQRIHREGVATTSEEMSLGACSLAVPVIRQTDDAVVAAIAVVVPTLKRDRQRLLGALQVAARGIGRLL

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=221, Percent_Identity=30.7692307692308, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082359, Length=235, Percent_Identity=27.2340425531915, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1786715, Length=220, Percent_Identity=29.0909090909091, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788131, Length=244, Percent_Identity=25.4098360655738, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788581, Length=216, Percent_Identity=29.1666666666667, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 25204; Mature: 25204

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNILGAFDERHNSLTLSELARRAGLPIATTHRLAAELVAGSALQRRADGQYVVGRLVWN
CCCCCCCHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
AGLLAPVEGQLRQVAEPFLYDVYAATLATVHLAVRDGDEVLYLERMMGRTSVPIVSAVGS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCCHHHHHHHC
RLPMHCTGVGKVLLAHAPEDVKDRVFATLTRITPYTIVSPTVLSSQMQRIHREGVATTSE
CCCEEECHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCCCHH
EMSLGACSLAVPVIRQTDDAVVAAIAVVVPTLKRDRQRLLGALQVAARGIGRLL
HHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLNILGAFDERHNSLTLSELARRAGLPIATTHRLAAELVAGSALQRRADGQYVVGRLVWN
CCCCCCCHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
AGLLAPVEGQLRQVAEPFLYDVYAATLATVHLAVRDGDEVLYLERMMGRTSVPIVSAVGS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCCHHHHHHHC
RLPMHCTGVGKVLLAHAPEDVKDRVFATLTRITPYTIVSPTVLSSQMQRIHREGVATTSE
CCCEEECHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCCCHH
EMSLGACSLAVPVIRQTDDAVVAAIAVVVPTLKRDRQRLLGALQVAARGIGRLL
HHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]