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The map label for this gene is yhjG [H]

Identifier: 119867614

GI number: 119867614

Start: 1682946

End: 1684382

Strand: Reverse

Name: yhjG [H]

Synonym: Mkms_1567

Alternate gene names: 119867614

Gene position: 1684382-1682946 (Counterclockwise)

Preceding gene: 119867615

Following gene: 119867613

Centisome position: 29.36

GC content: 69.94

Gene sequence:

>1437_bases
ATGACCACCGCGGTCGACGTCGTGATCGTCGGCGCCGGGCCGACCGGTCTGACCGCCGCTGGTGACCTGGCCCGCGCCGG
TCGCACCGTCGTGGTGTTCGAGAAACGGTCGACGCCGAATCCGTCGAGCCGCGCGTTCGCCACGATGGCACGCACGCTCG
AGGTCCTCGACGCGCGCGGCCTCGCCGGCGACCTGATCGCGCAGGGGCACCGCGCGCCGGGGGTGTCGCTCTTCGCCGGC
GCGCAGATCGATCTCAGCCACCTCGACTCGCCGTACCCGTTCGTGTTGGTCACCCCGCAGACCAACGTCGACCGCACCCT
TCTGGACTACGCCCAGCGGTACGGCGCCGACATCAGACGCGGCGTGGCAGTCGTGGGGATCGACCAGGACGCCGACGGTG
TGACGGTCACGGCCAGGTCCCAGGACTCGTCGCGTGACATCGCCTGGCGGGCAGACTATGTCGTCGGCGCGGACGGGGCG
CACAGCACGGTGCGCGAATTGCTGCGCATCGACTTCCCGGGTAGGACGATCCTGTCCTCGCTCGTCCTGGCCGACGTCAA
GCTGTCCGACGGCCCCGCCGGCGGAGGTCTGACCGTCGGCAGCACCCGCGACGTGTTCGGATTTCTGGCGCCCTATGACC
GCCACGATGCCGACGGATCCTGGTATCGCGCGATGTTGTGGGACCGCCACCACCAGGTTCCCGACCACGTGCCTGCCGAC
CGCACCGAGATCAGCGGGGTCCTCGCGCGGGCCTTCGACACCGACCCCGGTCTGGTGAACGTCGACTGGACGTCGCGATT
CCATTGCGACGAAAGGCAGGTCGCGCAGTACCGGCACGGCCGCGTGTTCCTCGCCGGCGACGCCGCGCACGTGCACTCCC
CCATGGGTGGGCAGGGGATGAACACCGGCATCCAGGACGCGGCCAACCTCGCTTGGAAACTCGACGCTGTGCTGGCCGGG
GCCGACGACGCCCTCCTCGACACCTACCACTGCGAGCGTCATCCGATCGGACGGCGGGTGCTGCGGCAGTCCGGGCTGAT
GGCACGAGGCATCACGCTTCGCCCGCGGGTGGCGCGGTGGGTGCGAAACAGGCTGGCGCCGAGGCTACTTCAGGTACCGA
TGATCCGCGACGGCGTAGCGGGCAGCTTCGCGGGGACCGGCCTTCGCTATCCGGGCGCCGGCCTCGTCGGAACCCGCGCC
ACCCAGATCCCGTTGACCGAGGGGCGACTTTCCGAACTGCTCCGCACGCCGGGTTTCGTACTGATCCGTGAACGCGGCGC
CGCCGTCGTGGACTCGTTCAGCGGACACCAGGCCGAACGCACCGACGATGGTCCGGCCGTGCTGATACGTCCCGACGGTT
ACGTCGCGTGGGCCGGGGATTCGGCGGACCATCCGCCGTGGGTGACGTGTCCTTCATCGTCAGCGGTGCGCGGATAG

Upstream 100 bases:

>100_bases
CTCATGTCAGCCAACCGGCCTGCCGCTTCGTGTTCAACCGCGAGGCCAAGGCCACCCCCGACGACATCCGCCGTTGGTAC
CCCGACCCGGAGGCGCACCG

Downstream 100 bases:

>100_bases
GGCAGCGATCGTCCGGCGATGCTGGATGATCCACACATGGACATCGCGCTGTGGGTGGTCGTGCTGCTCGAGTTCGCCGG
CCTGGTCACCCTCGGCGTCC

Product: FAD-binding monooxygenase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTTAVDVVIVGAGPTGLTAAGDLARAGRTVVVFEKRSTPNPSSRAFATMARTLEVLDARGLAGDLIAQGHRAPGVSLFAG
AQIDLSHLDSPYPFVLVTPQTNVDRTLLDYAQRYGADIRRGVAVVGIDQDADGVTVTARSQDSSRDIAWRADYVVGADGA
HSTVRELLRIDFPGRTILSSLVLADVKLSDGPAGGGLTVGSTRDVFGFLAPYDRHDADGSWYRAMLWDRHHQVPDHVPAD
RTEISGVLARAFDTDPGLVNVDWTSRFHCDERQVAQYRHGRVFLAGDAAHVHSPMGGQGMNTGIQDAANLAWKLDAVLAG
ADDALLDTYHCERHPIGRRVLRQSGLMARGITLRPRVARWVRNRLAPRLLQVPMIRDGVAGSFAGTGLRYPGAGLVGTRA
TQIPLTEGRLSELLRTPGFVLIRERGAAVVDSFSGHQAERTDDGPAVLIRPDGYVAWAGDSADHPPWVTCPSSSAVRG

Sequences:

>Translated_478_residues
MTTAVDVVIVGAGPTGLTAAGDLARAGRTVVVFEKRSTPNPSSRAFATMARTLEVLDARGLAGDLIAQGHRAPGVSLFAG
AQIDLSHLDSPYPFVLVTPQTNVDRTLLDYAQRYGADIRRGVAVVGIDQDADGVTVTARSQDSSRDIAWRADYVVGADGA
HSTVRELLRIDFPGRTILSSLVLADVKLSDGPAGGGLTVGSTRDVFGFLAPYDRHDADGSWYRAMLWDRHHQVPDHVPAD
RTEISGVLARAFDTDPGLVNVDWTSRFHCDERQVAQYRHGRVFLAGDAAHVHSPMGGQGMNTGIQDAANLAWKLDAVLAG
ADDALLDTYHCERHPIGRRVLRQSGLMARGITLRPRVARWVRNRLAPRLLQVPMIRDGVAGSFAGTGLRYPGAGLVGTRA
TQIPLTEGRLSELLRTPGFVLIRERGAAVVDSFSGHQAERTDDGPAVLIRPDGYVAWAGDSADHPPWVTCPSSSAVRG
>Mature_477_residues
TTAVDVVIVGAGPTGLTAAGDLARAGRTVVVFEKRSTPNPSSRAFATMARTLEVLDARGLAGDLIAQGHRAPGVSLFAGA
QIDLSHLDSPYPFVLVTPQTNVDRTLLDYAQRYGADIRRGVAVVGIDQDADGVTVTARSQDSSRDIAWRADYVVGADGAH
STVRELLRIDFPGRTILSSLVLADVKLSDGPAGGGLTVGSTRDVFGFLAPYDRHDADGSWYRAMLWDRHHQVPDHVPADR
TEISGVLARAFDTDPGLVNVDWTSRFHCDERQVAQYRHGRVFLAGDAAHVHSPMGGQGMNTGIQDAANLAWKLDAVLAGA
DDALLDTYHCERHPIGRRVLRQSGLMARGITLRPRVARWVRNRLAPRLLQVPMIRDGVAGSFAGTGLRYPGAGLVGTRAT
QIPLTEGRLSELLRTPGFVLIRERGAAVVDSFSGHQAERTDDGPAVLIRPDGYVAWAGDSADHPPWVTCPSSSAVRG

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=341, Percent_Identity=29.6187683284457, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 51244; Mature: 51113

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAVDVVIVGAGPTGLTAAGDLARAGRTVVVFEKRSTPNPSSRAFATMARTLEVLDARG
CCCEEEEEEECCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
LAGDLIAQGHRAPGVSLFAGAQIDLSHLDSPYPFVLVTPQTNVDRTLLDYAQRYGADIRR
CCHHHHHCCCCCCCEEEEECCEEEHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHC
GVAVVGIDQDADGVTVTARSQDSSRDIAWRADYVVGADGAHSTVRELLRIDFPGRTILSS
CEEEEEECCCCCCEEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHH
LVLADVKLSDGPAGGGLTVGSTRDVFGFLAPYDRHDADGSWYRAMLWDRHHQVPDHVPAD
HHHHEEEECCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCCC
RTEISGVLARAFDTDPGLVNVDWTSRFHCDERQVAQYRHGRVFLAGDAAHVHSPMGGQGM
HHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHCCEEEEEECHHHHCCCCCCCCC
NTGIQDAANLAWKLDAVLAGADDALLDTYHCERHPIGRRVLRQSGLMARGITLRPRVARW
CCCHHHHHHHHEEEEEEEECCCHHHHHHHCCCCCCHHHHHHHHCCCEECCCEECHHHHHH
VRNRLAPRLLQVPMIRDGVAGSFAGTGLRYPGAGLVGTRATQIPLTEGRLSELLRTPGFV
HHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCEE
LIRERGAAVVDSFSGHQAERTDDGPAVLIRPDGYVAWAGDSADHPPWVTCPSSSAVRG
EEECCCCEEEECCCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
TTAVDVVIVGAGPTGLTAAGDLARAGRTVVVFEKRSTPNPSSRAFATMARTLEVLDARG
CCEEEEEEECCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
LAGDLIAQGHRAPGVSLFAGAQIDLSHLDSPYPFVLVTPQTNVDRTLLDYAQRYGADIRR
CCHHHHHCCCCCCCEEEEECCEEEHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHC
GVAVVGIDQDADGVTVTARSQDSSRDIAWRADYVVGADGAHSTVRELLRIDFPGRTILSS
CEEEEEECCCCCCEEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHH
LVLADVKLSDGPAGGGLTVGSTRDVFGFLAPYDRHDADGSWYRAMLWDRHHQVPDHVPAD
HHHHEEEECCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCCC
RTEISGVLARAFDTDPGLVNVDWTSRFHCDERQVAQYRHGRVFLAGDAAHVHSPMGGQGM
HHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHCCEEEEEECHHHHCCCCCCCCC
NTGIQDAANLAWKLDAVLAGADDALLDTYHCERHPIGRRVLRQSGLMARGITLRPRVARW
CCCHHHHHHHHEEEEEEEECCCHHHHHHHCCCCCCHHHHHHHHCCCEECCCEECHHHHHH
VRNRLAPRLLQVPMIRDGVAGSFAGTGLRYPGAGLVGTRATQIPLTEGRLSELLRTPGFV
HHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCEE
LIRERGAAVVDSFSGHQAERTDDGPAVLIRPDGYVAWAGDSADHPPWVTCPSSSAVRG
EEECCCCEEEECCCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]