Click here to switch to the map view.

The map label for this gene is fadA [H]

Identifier: 119867588

GI number: 119867588

Start: 1655745

End: 1656956

Strand: Reverse

Name: fadA [H]

Synonym: Mkms_1540

Alternate gene names: 119867588

Gene position: 1656956-1655745 (Counterclockwise)

Preceding gene: 119867589

Following gene: 119867587

Centisome position: 28.88

GC content: 67.24

Gene sequence:

>1212_bases
ATGCCTGAAGAAGCCTTCATCTACGAAGCGATCCGCACGCCGCGCGGTAAGCAGCGCGGTGGCGCTCTGCACGAGGTCAA
GCCGATCAGTCTGGTTGTCGGTCTGGTTGACGAGATCCGCTCGCGATTCCCCGATTTGGATGAGTCCCTGATCTCCGACC
TGATCCTGGGTGTGGTGTCCCCACTGGGCGATCAGGGCGCTGTGCTTGCCCGCACCGCGGTGCTGGCGGCCAAGTTGCCC
GATACCACCGGCGGCGTGCAGCTCAACCGGTTCTGCGGATCGGGCCTGGAGGCCGTGAACACCGCCGCGCAGAAGGTGCG
CTCGGGCTGGGACGACCTGGTGCTGGCCGGTGGCGTGGAGTCGATGAGCCGCGTGCCGATGGGCTCCGACGGCGGCGCCT
GGGCCAACGACCCGGAGACCAACTACGCGGTGTCCTTTGTCCCGCAGGGCATCGGCGCCGACCTGATCGCCACCATGGAG
GGCTTCTCGCGCGAGGATGTCGACGCCTACGCGGTGCGCAGCCAGCAGCGTGCCGCGGCAGCATGGTCCGGCGGCTACTT
CGCCAAATCGGTTGTGCCGGTCAAGGATCAGAACGGCTTGGTCATCCTGGATCACGACGAGCACATGCGCCCGAACACCA
CCCTGGAGAGCCTGAGCAAGCTCAAGCCCGCGTTCACCGATATCGGCGCGATGGGCGGCTTCAACGAGGTCGCGCTGCAG
AAGTACCACACGGTGGAGAAGATCAACCACGTCCACCACGGTGGTAACAGTTCGGGCATCGTCGACGGCGCCGCCCTGGT
GCTGATCGGGAGCGAGAACGCCGGTGTCTCGCAGGGGCTGACGCCGCGGGCGCGCATCGTGGCGACGGCCACCAGCGGCG
CCGACGGCACGATCATGCTGACGGGGCCGACCCCGGCCACCCGCAAGGTGCTCGACCGGGCCGGTCTGACCGTCGACGAC
ATCGACCTGTTCGAACTGAACGAGGCCTTCGCCTCGGTGGTGCTCAAGTTCCAGAAGGACCTGGGCATCCCCGACGAGAA
ACTGAACGTCAACGGTGGCGCCATCGCGATGGGCCACCCCCTGGGCGCCACCGGCGCCATGATCACCGGAACCATGGTCG
ACGAGCTCGAGCGTCGCGGCGCCAAACGCGCGCTGATCACGCTGTGCATCGGCGGCGGCATGGGCGTGGCCACCATCATC
GAGCGCGTCTAG

Upstream 100 bases:

>100_bases
GGCCGCGTCGTCACCAACTCGCCGTGGGCCGTTCCCGACTACTTTGCCCGCACGTCCCAACCGAATATGGCGGACTACAC
CATCACCCGAGGAAACACAG

Downstream 100 bases:

>100_bases
GAGTACGTGAAAATGAGCACAGAGAACACCATTGCGTGGGATGAGGATGCCGACGGCATCGTCACGCTGACACTCGACGA
CCCGACCGGTTCGGCCAACG

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MPEEAFIYEAIRTPRGKQRGGALHEVKPISLVVGLVDEIRSRFPDLDESLISDLILGVVSPLGDQGAVLARTAVLAAKLP
DTTGGVQLNRFCGSGLEAVNTAAQKVRSGWDDLVLAGGVESMSRVPMGSDGGAWANDPETNYAVSFVPQGIGADLIATME
GFSREDVDAYAVRSQQRAAAAWSGGYFAKSVVPVKDQNGLVILDHDEHMRPNTTLESLSKLKPAFTDIGAMGGFNEVALQ
KYHTVEKINHVHHGGNSSGIVDGAALVLIGSENAGVSQGLTPRARIVATATSGADGTIMLTGPTPATRKVLDRAGLTVDD
IDLFELNEAFASVVLKFQKDLGIPDEKLNVNGGAIAMGHPLGATGAMITGTMVDELERRGAKRALITLCIGGGMGVATII
ERV

Sequences:

>Translated_403_residues
MPEEAFIYEAIRTPRGKQRGGALHEVKPISLVVGLVDEIRSRFPDLDESLISDLILGVVSPLGDQGAVLARTAVLAAKLP
DTTGGVQLNRFCGSGLEAVNTAAQKVRSGWDDLVLAGGVESMSRVPMGSDGGAWANDPETNYAVSFVPQGIGADLIATME
GFSREDVDAYAVRSQQRAAAAWSGGYFAKSVVPVKDQNGLVILDHDEHMRPNTTLESLSKLKPAFTDIGAMGGFNEVALQ
KYHTVEKINHVHHGGNSSGIVDGAALVLIGSENAGVSQGLTPRARIVATATSGADGTIMLTGPTPATRKVLDRAGLTVDD
IDLFELNEAFASVVLKFQKDLGIPDEKLNVNGGAIAMGHPLGATGAMITGTMVDELERRGAKRALITLCIGGGMGVATII
ERV
>Mature_402_residues
PEEAFIYEAIRTPRGKQRGGALHEVKPISLVVGLVDEIRSRFPDLDESLISDLILGVVSPLGDQGAVLARTAVLAAKLPD
TTGGVQLNRFCGSGLEAVNTAAQKVRSGWDDLVLAGGVESMSRVPMGSDGGAWANDPETNYAVSFVPQGIGADLIATMEG
FSREDVDAYAVRSQQRAAAAWSGGYFAKSVVPVKDQNGLVILDHDEHMRPNTTLESLSKLKPAFTDIGAMGGFNEVALQK
YHTVEKINHVHHGGNSSGIVDGAALVLIGSENAGVSQGLTPRARIVATATSGADGTIMLTGPTPATRKVLDRAGLTVDDI
DLFELNEAFASVVLKFQKDLGIPDEKLNVNGGAIAMGHPLGATGAMITGTMVDELERRGAKRALITLCIGGGMGVATIIE
RV

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=417, Percent_Identity=36.6906474820144, Blast_Score=234, Evalue=7e-62,
Organism=Homo sapiens, GI148539872, Length=409, Percent_Identity=37.1638141809291, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI4501853, Length=367, Percent_Identity=38.6920980926431, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI4557237, Length=355, Percent_Identity=33.5211267605634, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI4504327, Length=379, Percent_Identity=31.6622691292876, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI194353979, Length=90, Percent_Identity=54.4444444444444, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1787663, Length=422, Percent_Identity=44.0758293838863, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI48994986, Length=407, Percent_Identity=39.8034398034398, Blast_Score=277, Evalue=7e-76,
Organism=Escherichia coli, GI87082165, Length=423, Percent_Identity=37.1158392434988, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1788554, Length=416, Percent_Identity=37.2596153846154, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1788683, Length=431, Percent_Identity=29.4663573085847, Blast_Score=135, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI133906874, Length=417, Percent_Identity=37.8896882494005, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17535921, Length=416, Percent_Identity=32.6923076923077, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI25147385, Length=414, Percent_Identity=30.9178743961353, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17551802, Length=452, Percent_Identity=28.0973451327434, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17535917, Length=404, Percent_Identity=26.980198019802, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322031, Length=406, Percent_Identity=36.6995073891626, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6325229, Length=422, Percent_Identity=28.6729857819905, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24655093, Length=414, Percent_Identity=37.1980676328502, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI17648125, Length=360, Percent_Identity=36.1111111111111, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24640423, Length=373, Percent_Identity=30.8310991957105, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17137578, Length=404, Percent_Identity=29.7029702970297, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42219; Mature: 42087

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEEAFIYEAIRTPRGKQRGGALHEVKPISLVVGLVDEIRSRFPDLDESLISDLILGVVS
CCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
PLGDQGAVLARTAVLAAKLPDTTGGVQLNRFCGSGLEAVNTAAQKVRSGWDDLVLAGGVE
CCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEECCHH
SMSRVPMGSDGGAWANDPETNYAVSFVPQGIGADLIATMEGFSREDVDAYAVRSQQRAAA
HHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AWSGGYFAKSVVPVKDQNGLVILDHDEHMRPNTTLESLSKLKPAFTDIGAMGGFNEVALQ
HCCCCCHHCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHCHHHHHHHCCCCCHHHHHH
KYHTVEKINHVHHGGNSSGIVDGAALVLIGSENAGVSQGLTPRARIVATATSGADGTIML
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCEEEE
TGPTPATRKVLDRAGLTVDDIDLFELNEAFASVVLKFQKDLGIPDEKLNVNGGAIAMGHP
ECCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCC
LGATGAMITGTMVDELERRGAKRALITLCIGGGMGVATIIERV
CCCCCCEEEHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
PEEAFIYEAIRTPRGKQRGGALHEVKPISLVVGLVDEIRSRFPDLDESLISDLILGVVS
CCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
PLGDQGAVLARTAVLAAKLPDTTGGVQLNRFCGSGLEAVNTAAQKVRSGWDDLVLAGGVE
CCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEECCHH
SMSRVPMGSDGGAWANDPETNYAVSFVPQGIGADLIATMEGFSREDVDAYAVRSQQRAAA
HHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AWSGGYFAKSVVPVKDQNGLVILDHDEHMRPNTTLESLSKLKPAFTDIGAMGGFNEVALQ
HCCCCCHHCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHCHHHHHHHCCCCCHHHHHH
KYHTVEKINHVHHGGNSSGIVDGAALVLIGSENAGVSQGLTPRARIVATATSGADGTIML
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCEEEE
TGPTPATRKVLDRAGLTVDDIDLFELNEAFASVVLKFQKDLGIPDEKLNVNGGAIAMGHP
ECCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCC
LGATGAMITGTMVDELERRGAKRALITLCIGGGMGVATIIERV
CCCCCCEEEHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]