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The map label for this gene is uvrD [H]

Identifier: 119867477

GI number: 119867477

Start: 1529285

End: 1531408

Strand: Direct

Name: uvrD [H]

Synonym: Mkms_1427

Alternate gene names: 119867477

Gene position: 1529285-1531408 (Clockwise)

Preceding gene: 119867475

Following gene: 119867478

Centisome position: 26.66

GC content: 72.55

Gene sequence:

>2124_bases
ATGGGTCGCATGCCAGTCGAGGTCCCGACCTCGTCGCGGGAGCGGCTGCTTGCCGACCTCGACGAGGAACAGCGTGAGGC
CGTCCTGGCGCCTCGGGGGCCGGTCTGCGTGCTGGCCGGCGCCGGCACCGGCAAGACCCGCACCATCACCCGCCGGATCG
CCCACCTTGTCGCGGCGGGCCATGTCGCACCCGGGCAGGTGCTGGCCGTGACGTTCACCTCCCGCGCCGCGGGGGAGATG
CGGGCCCGGCTGCGGGCGCTCGACCAGGAGGCCGGCGGCGTCGGCACCGGGTCGGTACAGGCGATGACGTTCCACGCCGC
CGCCCGCCGCCAGCTGCGGTACTTCTGGCCCCGCGTGGTCGGCGACACCTCCTGGCAGCTGCTCGACAGCAAGTTCTCCG
TCGTGGCGCAGGCCGCCAACCGGGCTCGCGTGCAGGCCGCCACCGACGACGTCCGCGACCTGGCCGGTGAGATCGAGTGG
GCGAAGGCGTCGCTGATCAGCCCGGAGGCCTACCCCGGCGCCGTCGCGCAGGCCGGCCGCGACATCCCGATGGACGCCGC
GAAGGTGGCCGCCGTCTACGCCGGTTACGAGGCGCTCAAGGCCCGCCACGACGGCACCGCGCTGCTCGATTTCGACGATC
TGCTGCTGCACACCGCGGCGGCGATCGAGAACGACGCGGCGGTGGCCGCGGAGTTCCGTGACCGCTACCGCTGTTTCGTC
GTCGACGAGTACCAGGACGTGACACCCCTGCAACAGCGCGTGCTCGACGCGTGGCTCGGCCCCCGTGACGACCTGACCGT
CGTCGGGGACGCCAACCAGACCATCTACTCGTTCACCGGGGCCACGCCGCGCTACCTGCTGGACTTCTCCCGCCGGTTCC
CCGACGCCGCGGTGATCCGGCTCGAACGCGACTACCGCTCCACCCCGCAGGTGGTGTCGCTGGCCAACCGGGTGATCGCC
GCCGCGCGCGGCCGCATGGCCGGCAGCAAGCTGCACCTGGTCGGCCAGCGCCCGGCGGGGCCCACACCGGCGTTCGCCGA
GCACACCGACGAGGTGGCCGAGGCCGCCGCCGTCGCCAAGGACATCCGCAGGCTGCTCGACGCCGGCACCCCCGCGGCGG
AGATCGCGGTGCTGTACCGCATCAACGCGCAGTCGGAGGTCTACGAGGAGGCGCTCACCGAGGCCGGCATCGCGTTCCAG
GTGCGCGGTGGTGAGGGCTTCTTCAGCCGTCAGGAGATCCGCCAGGCGCTCGTGGCCCTGCAGCGGGCCGCCGAACGCGA
CGTCGACGGTGAGGTGGCCGACGTCGTGCGGGCCGTGCTCGAACCGCTCGGGCTCACCGCGGAGCCGCCGGCGGGCACCC
GCGCCAGGGAACGCTGGGAGGCGCTCAGCGCGCTGGCCGAACTCGTCGACGAAGAGGTCGTGCTGCGCCCGGGTCTTGAC
CTGCGCGGTCTGGTCACCGAGCTGCGCCAGCGCGCCGACGCCCGGCACCCACCGGTGGTCCAGGGCGTCACGCTCGCGTC
GCTGCATGCGGCGAAGGGGTTGGAGTGGGATGCGGTCTATCTCGTCGGGCTCGCCGACGGCACCCTGCCGATCTCGCACG
CGCTGACCCACGGCCCCGACAGTGAGCCGGTCGAGGAGGAGCGCCGGTTGCTCTATGTCGGAATCACAAGGGCGCGAGTG
CATCTCACATTGAGCTGGGCGCTGGCGCGGGCACCGGGGGGCCGCCAGGGTCGACGGCCGTCGCGGTTCCTCAACGGTGT
GGCGCCGCACTCGGCACCCGAGGCGTCGGTGAACAAACCGCGTCGCGCGCGGGGCGCGACGCCGCGCTGCCGGGTCTGCA
ACCAGGTGTTGACCACCCCCGCGGCAATCATGTTGCGCCGCTGCGAGACCTGCTCGTCCGATATCGACGAACAACTGCTC
GCCGCGCTCAAGGAGTGGCGGCTGCGGACCTCCAAGGAGCTCGGCGTCCCGGCCTTCGTGGTGTTCACCGACAACACGCT
GATCGCGATCGCCGAATCGCTGCCCACCGACGAGGCGGCGCTGGTCGCCATCCCCGGAATCGGCGCCCGCAAGCTCGAGC
AGTACGGCGCCGACGTTCTCGCCTTGGTCAACGCACGCGGCTGA

Upstream 100 bases:

>100_bases
CATCGTCAGGGTGTCAGCAGCACTCATAAGGCATGCAACGTTACCGACCGCCCCGACATGCCCGACACGGGCCGGGCGGT
TGTCGGTCCGCCCTGCCAAG

Downstream 100 bases:

>100_bases
GACTGCCAACCACCGCAAAGATCGCGCCAAAAGCGCTGCTAGAAAATAGGTTGTGCGGTTCCGCTGCTAGGCTCTAGCCT
CAAGATCACACGTTGGGCGG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 707; Mature: 706

Protein sequence:

>707_residues
MGRMPVEVPTSSRERLLADLDEEQREAVLAPRGPVCVLAGAGTGKTRTITRRIAHLVAAGHVAPGQVLAVTFTSRAAGEM
RARLRALDQEAGGVGTGSVQAMTFHAAARRQLRYFWPRVVGDTSWQLLDSKFSVVAQAANRARVQAATDDVRDLAGEIEW
AKASLISPEAYPGAVAQAGRDIPMDAAKVAAVYAGYEALKARHDGTALLDFDDLLLHTAAAIENDAAVAAEFRDRYRCFV
VDEYQDVTPLQQRVLDAWLGPRDDLTVVGDANQTIYSFTGATPRYLLDFSRRFPDAAVIRLERDYRSTPQVVSLANRVIA
AARGRMAGSKLHLVGQRPAGPTPAFAEHTDEVAEAAAVAKDIRRLLDAGTPAAEIAVLYRINAQSEVYEEALTEAGIAFQ
VRGGEGFFSRQEIRQALVALQRAAERDVDGEVADVVRAVLEPLGLTAEPPAGTRARERWEALSALAELVDEEVVLRPGLD
LRGLVTELRQRADARHPPVVQGVTLASLHAAKGLEWDAVYLVGLADGTLPISHALTHGPDSEPVEEERRLLYVGITRARV
HLTLSWALARAPGGRQGRRPSRFLNGVAPHSAPEASVNKPRRARGATPRCRVCNQVLTTPAAIMLRRCETCSSDIDEQLL
AALKEWRLRTSKELGVPAFVVFTDNTLIAIAESLPTDEAALVAIPGIGARKLEQYGADVLALVNARG

Sequences:

>Translated_707_residues
MGRMPVEVPTSSRERLLADLDEEQREAVLAPRGPVCVLAGAGTGKTRTITRRIAHLVAAGHVAPGQVLAVTFTSRAAGEM
RARLRALDQEAGGVGTGSVQAMTFHAAARRQLRYFWPRVVGDTSWQLLDSKFSVVAQAANRARVQAATDDVRDLAGEIEW
AKASLISPEAYPGAVAQAGRDIPMDAAKVAAVYAGYEALKARHDGTALLDFDDLLLHTAAAIENDAAVAAEFRDRYRCFV
VDEYQDVTPLQQRVLDAWLGPRDDLTVVGDANQTIYSFTGATPRYLLDFSRRFPDAAVIRLERDYRSTPQVVSLANRVIA
AARGRMAGSKLHLVGQRPAGPTPAFAEHTDEVAEAAAVAKDIRRLLDAGTPAAEIAVLYRINAQSEVYEEALTEAGIAFQ
VRGGEGFFSRQEIRQALVALQRAAERDVDGEVADVVRAVLEPLGLTAEPPAGTRARERWEALSALAELVDEEVVLRPGLD
LRGLVTELRQRADARHPPVVQGVTLASLHAAKGLEWDAVYLVGLADGTLPISHALTHGPDSEPVEEERRLLYVGITRARV
HLTLSWALARAPGGRQGRRPSRFLNGVAPHSAPEASVNKPRRARGATPRCRVCNQVLTTPAAIMLRRCETCSSDIDEQLL
AALKEWRLRTSKELGVPAFVVFTDNTLIAIAESLPTDEAALVAIPGIGARKLEQYGADVLALVNARG
>Mature_706_residues
GRMPVEVPTSSRERLLADLDEEQREAVLAPRGPVCVLAGAGTGKTRTITRRIAHLVAAGHVAPGQVLAVTFTSRAAGEMR
ARLRALDQEAGGVGTGSVQAMTFHAAARRQLRYFWPRVVGDTSWQLLDSKFSVVAQAANRARVQAATDDVRDLAGEIEWA
KASLISPEAYPGAVAQAGRDIPMDAAKVAAVYAGYEALKARHDGTALLDFDDLLLHTAAAIENDAAVAAEFRDRYRCFVV
DEYQDVTPLQQRVLDAWLGPRDDLTVVGDANQTIYSFTGATPRYLLDFSRRFPDAAVIRLERDYRSTPQVVSLANRVIAA
ARGRMAGSKLHLVGQRPAGPTPAFAEHTDEVAEAAAVAKDIRRLLDAGTPAAEIAVLYRINAQSEVYEEALTEAGIAFQV
RGGEGFFSRQEIRQALVALQRAAERDVDGEVADVVRAVLEPLGLTAEPPAGTRARERWEALSALAELVDEEVVLRPGLDL
RGLVTELRQRADARHPPVVQGVTLASLHAAKGLEWDAVYLVGLADGTLPISHALTHGPDSEPVEEERRLLYVGITRARVH
LTLSWALARAPGGRQGRRPSRFLNGVAPHSAPEASVNKPRRARGATPRCRVCNQVLTTPAAIMLRRCETCSSDIDEQLLA
ALKEWRLRTSKELGVPAFVVFTDNTLIAIAESLPTDEAALVAIPGIGARKLEQYGADVLALVNARG

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=685, Percent_Identity=32.2627737226277, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI48994965, Length=444, Percent_Identity=30.4054054054054, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322369, Length=437, Percent_Identity=26.0869565217391, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324477, Length=395, Percent_Identity=21.2658227848101, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR002121
- InterPro:   IPR014016
- InterPro:   IPR010997 [H]

Pfam domain/function: PF00570 HRDC; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76382; Mature: 76250

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50967 HRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRMPVEVPTSSRERLLADLDEEQREAVLAPRGPVCVLAGAGTGKTRTITRRIAHLVAAG
CCCCCCCCCCCHHHHHHHHCCHHHHHEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
HVAPGQVLAVTFTSRAAGEMRARLRALDQEAGGVGTGSVQAMTFHAAARRQLRYFWPRVV
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
GDTSWQLLDSKFSVVAQAANRARVQAATDDVRDLAGEIEWAKASLISPEAYPGAVAQAGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHCCC
DIPMDAAKVAAVYAGYEALKARHDGTALLDFDDLLLHTAAAIENDAAVAAEFRDRYRCFV
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHCCEEEEE
VDEYQDVTPLQQRVLDAWLGPRDDLTVVGDANQTIYSFTGATPRYLLDFSRRFPDAAVIR
EECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCCCEEEEE
LERDYRSTPQVVSLANRVIAAARGRMAGSKLHLVGQRPAGPTPAFAEHTDEVAEAAAVAK
EECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
DIRRLLDAGTPAAEIAVLYRINAQSEVYEEALTEAGIAFQVRGGEGFFSRQEIRQALVAL
HHHHHHHCCCCHHHEEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHH
QRAAERDVDGEVADVVRAVLEPLGLTAEPPAGTRARERWEALSALAELVDEEVVLRPGLD
HHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
LRGLVTELRQRADARHPPVVQGVTLASLHAAKGLEWDAVYLVGLADGTLPISHALTHGPD
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHCCCCC
SEPVEEERRLLYVGITRARVHLTLSWALARAPGGRQGRRPSRFLNGVAPHSAPEASVNKP
CCCHHHCCCEEEEEEEEEEEEEEEEEHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCC
RRARGATPRCRVCNQVLTTPAAIMLRRCETCSSDIDEQLLAALKEWRLRTSKELGVPAFV
HHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VFTDNTLIAIAESLPTDEAALVAIPGIGARKLEQYGADVLALVNARG
EEECCEEEEEECCCCCCCEEEEEECCCCHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure 
GRMPVEVPTSSRERLLADLDEEQREAVLAPRGPVCVLAGAGTGKTRTITRRIAHLVAAG
CCCCCCCCCCHHHHHHHHCCHHHHHEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
HVAPGQVLAVTFTSRAAGEMRARLRALDQEAGGVGTGSVQAMTFHAAARRQLRYFWPRVV
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
GDTSWQLLDSKFSVVAQAANRARVQAATDDVRDLAGEIEWAKASLISPEAYPGAVAQAGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHCCC
DIPMDAAKVAAVYAGYEALKARHDGTALLDFDDLLLHTAAAIENDAAVAAEFRDRYRCFV
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHCCEEEEE
VDEYQDVTPLQQRVLDAWLGPRDDLTVVGDANQTIYSFTGATPRYLLDFSRRFPDAAVIR
EECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCCCEEEEE
LERDYRSTPQVVSLANRVIAAARGRMAGSKLHLVGQRPAGPTPAFAEHTDEVAEAAAVAK
EECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
DIRRLLDAGTPAAEIAVLYRINAQSEVYEEALTEAGIAFQVRGGEGFFSRQEIRQALVAL
HHHHHHHCCCCHHHEEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHH
QRAAERDVDGEVADVVRAVLEPLGLTAEPPAGTRARERWEALSALAELVDEEVVLRPGLD
HHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
LRGLVTELRQRADARHPPVVQGVTLASLHAAKGLEWDAVYLVGLADGTLPISHALTHGPD
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHCCCCC
SEPVEEERRLLYVGITRARVHLTLSWALARAPGGRQGRRPSRFLNGVAPHSAPEASVNKP
CCCHHHCCCEEEEEEEEEEEEEEEEEHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCC
RRARGATPRCRVCNQVLTTPAAIMLRRCETCSSDIDEQLLAALKEWRLRTSKELGVPAFV
HHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VFTDNTLIAIAESLPTDEAALVAIPGIGARKLEQYGADVLALVNARG
EEECCEEEEEECCCCCCCEEEEEECCCCHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]