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The map label for this gene is yogA [H]

Identifier: 119867459

GI number: 119867459

Start: 1508635

End: 1509567

Strand: Reverse

Name: yogA [H]

Synonym: Mkms_1409

Alternate gene names: 119867459

Gene position: 1509567-1508635 (Counterclockwise)

Preceding gene: 119867460

Following gene: 119867458

Centisome position: 26.31

GC content: 72.67

Gene sequence:

>933_bases
ATGCGCGCGGTCATTGCGCGTGACCGCGCAGCCGGTGTCCACGGTCTGTCGATGGCAGACGTGCCGTACCCGCATGCGGC
GGAGAACGACGTCATCGTCCGGGTACACGCCGCGAGTTTCACACCGGGTGAACTCGACTGGCCTGCAACATGGTTGGACC
GCGCCGGCCGGGACCGCGCGCCGACGGTCCCCGGCCACGAGGTGTCCGGGGTCATCGCGGAGCTGGGGTATGGCACCACA
GGGCTGACCGTGGGCCAGCGCGTCTTCGGGGTCACCGACTGGGCCCGCGACGGCACGCTCGCCGAATTCGTCGCGGCGGA
GGCGCGCAACCTGGCGCCGCTCGCCGCGGACGTCGACCACCCGGTGGCGGCCGCCCTGCCGATCTCCGGGCTCACGGCGT
GGCAGGCCCTGTTCGACCACGCGCACCTCACCGCGGGACAGACCGTGCTGATCCACGGCGCCGGCGGCGCGGTCGGGTGG
ATCGCGGTGCAGTTGGCCCGTGAGGCGGGTGCCCGGGTCATCGGGAGCGGACGTGCGCACCACCGGGACGCCGTGCTCGG
CCTGGGTGCCGACGCCTTTCTGGACCTGCAGCGCGATTCCGTCGAGGACTGCGGCCCGGTCGACGTGGTGCTCACGGTGA
TCGGCGGAGAGATCCAGGACCGTTCGGCGGCCGCCGTGCGACCGGGTGGCGTGATGGTGAGCATCGCCGAACCCCCCGGG
ACGGTGCCCGACCGCGGACGGGCCGTCTTCTTCGTCGTGGAACCCGACCGCGCCGGACTGCTCCGGCTACAGGACCGGGT
CCGCGACGGCAGGCTTCGCCCACCCCCGATGACGGTGTGCACGCTCGACGAGGCACCCGCGGCGTTCGACCCAGCCCGGC
GCCGGGCGGGTAAGACCGTGGTCGTCGTCGACGATCACTCCATCACGAGGTAA

Upstream 100 bases:

>100_bases
ACCGAATCGCGGCGCCTGTCTCGACAACGGGTACGTCGCCGCCACGACGGCGTTACCGTTGACGACATGTCGGTTCAGTG
TTCGCCACACCCGACCACAG

Downstream 100 bases:

>100_bases
TTCATGACGAAAAAGGTTGCCGGAGTTGAGTTCCCGGACTCCACGCTGGCCCGGGAGGCCACCGGGATCGTTCGCGGCGC
CGCCGACGATCTGTTGTTCG

Product: alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRAVIARDRAAGVHGLSMADVPYPHAAENDVIVRVHAASFTPGELDWPATWLDRAGRDRAPTVPGHEVSGVIAELGYGTT
GLTVGQRVFGVTDWARDGTLAEFVAAEARNLAPLAADVDHPVAAALPISGLTAWQALFDHAHLTAGQTVLIHGAGGAVGW
IAVQLAREAGARVIGSGRAHHRDAVLGLGADAFLDLQRDSVEDCGPVDVVLTVIGGEIQDRSAAAVRPGGVMVSIAEPPG
TVPDRGRAVFFVVEPDRAGLLRLQDRVRDGRLRPPPMTVCTLDEAPAAFDPARRRAGKTVVVVDDHSITR

Sequences:

>Translated_310_residues
MRAVIARDRAAGVHGLSMADVPYPHAAENDVIVRVHAASFTPGELDWPATWLDRAGRDRAPTVPGHEVSGVIAELGYGTT
GLTVGQRVFGVTDWARDGTLAEFVAAEARNLAPLAADVDHPVAAALPISGLTAWQALFDHAHLTAGQTVLIHGAGGAVGW
IAVQLAREAGARVIGSGRAHHRDAVLGLGADAFLDLQRDSVEDCGPVDVVLTVIGGEIQDRSAAAVRPGGVMVSIAEPPG
TVPDRGRAVFFVVEPDRAGLLRLQDRVRDGRLRPPPMTVCTLDEAPAAFDPARRRAGKTVVVVDDHSITR
>Mature_310_residues
MRAVIARDRAAGVHGLSMADVPYPHAAENDVIVRVHAASFTPGELDWPATWLDRAGRDRAPTVPGHEVSGVIAELGYGTT
GLTVGQRVFGVTDWARDGTLAEFVAAEARNLAPLAADVDHPVAAALPISGLTAWQALFDHAHLTAGQTVLIHGAGGAVGW
IAVQLAREAGARVIGSGRAHHRDAVLGLGADAFLDLQRDSVEDCGPVDVVLTVIGGEIQDRSAAAVRPGGVMVSIAEPPG
TVPDRGRAVFFVVEPDRAGLLRLQDRVRDGRLRPPPMTVCTLDEAPAAFDPARRRAGKTVVVVDDHSITR

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=269, Percent_Identity=28.6245353159851, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI194239674, Length=229, Percent_Identity=27.9475982532751, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI13236495, Length=229, Percent_Identity=27.9475982532751, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI194239676, Length=181, Percent_Identity=32.0441988950276, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI24308257, Length=248, Percent_Identity=26.6129032258064, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI18379349, Length=181, Percent_Identity=29.8342541436464, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI22538446, Length=232, Percent_Identity=27.1551724137931, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI22538444, Length=232, Percent_Identity=27.1551724137931, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI28557745, Length=224, Percent_Identity=27.6785714285714, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1790485, Length=261, Percent_Identity=25.6704980842912, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71987554, Length=227, Percent_Identity=29.5154185022026, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319621, Length=264, Percent_Identity=26.5151515151515, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324486, Length=267, Percent_Identity=24.3445692883895, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 32555; Mature: 32555

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVIARDRAAGVHGLSMADVPYPHAAENDVIVRVHAASFTPGELDWPATWLDRAGRDRA
CCCEECCCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCC
PTVPGHEVSGVIAELGYGTTGLTVGQRVFGVTDWARDGTLAEFVAAEARNLAPLAADVDH
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEECCCCC
PVAAALPISGLTAWQALFDHAHLTAGQTVLIHGAGGAVGWIAVQLAREAGARVIGSGRAH
CEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCC
HRDAVLGLGADAFLDLQRDSVEDCGPVDVVLTVIGGEIQDRSAAAVRPGGVMVSIAEPPG
CCCEEEECCCHHEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCEEEEECCCCC
TVPDRGRAVFFVVEPDRAGLLRLQDRVRDGRLRPPPMTVCTLDEAPAAFDPARRRAGKTV
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHCCCEE
VVVDDHSITR
EEECCCCCCC
>Mature Secondary Structure
MRAVIARDRAAGVHGLSMADVPYPHAAENDVIVRVHAASFTPGELDWPATWLDRAGRDRA
CCCEECCCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCC
PTVPGHEVSGVIAELGYGTTGLTVGQRVFGVTDWARDGTLAEFVAAEARNLAPLAADVDH
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEECCCCC
PVAAALPISGLTAWQALFDHAHLTAGQTVLIHGAGGAVGWIAVQLAREAGARVIGSGRAH
CEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCC
HRDAVLGLGADAFLDLQRDSVEDCGPVDVVLTVIGGEIQDRSAAAVRPGGVMVSIAEPPG
CCCEEEECCCHHEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCEEEEECCCCC
TVPDRGRAVFFVVEPDRAGLLRLQDRVRDGRLRPPPMTVCTLDEAPAAFDPARRRAGKTV
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHCCCEE
VVVDDHSITR
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]