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The map label for this gene is rpoE [H]

Identifier: 119867447

GI number: 119867447

Start: 1499852

End: 1500565

Strand: Direct

Name: rpoE [H]

Synonym: Mkms_1397

Alternate gene names: 119867447

Gene position: 1499852-1500565 (Clockwise)

Preceding gene: 119867445

Following gene: 119867448

Centisome position: 26.14

GC content: 66.39

Gene sequence:

>714_bases
GTGCCAACGGCCTGCCTGGATCGTCCGGTGGATCTGCCGGGCCTGTTCGACGGCGCCGCGACAGAAGGGACGGTGTCGAC
CACGATGACCGACATCGACGGTGCGGCGGCCGAACGACAGGAAACCGACGCCGAACTGACCGCGCGTTTCGAGCGCGATG
CGATACCGCTGCTCGACCAGCTCTACGGCGGCGCGCTGCGCATGACGCGCAATCCCGCCGACGCCGAGGATCTGCTGCAG
GAGACGATGGTCAAGGCCTATGCCGGGTTCCGGTCGTTCCGCGAGGGCACCAACCTCAAGGCGTGGCTATACCGCATCCT
GACCAACACCTACATCAACAGCTACCGCAAGAAGCAGCGCCAGCCGTCGGAGTACCCCACCGAGGAGATCACGGACTGGC
AGCTCGCCGCGAACGCCGAGCATTCGTCGACGGGGCTGCGGTCTGCCGAGGTCGAGGCGCTCGAATCGCTGCCCGACACG
GAGATCAAGGCGGCGTTGCAGGCGCTGCCGGAGGATTTCCGGATGGCGGTGTACTACGCCGACGTCGAAGGATTCCCGTA
CAAGGAGATCGCGGAGATCATGGATACGCCCATCGGGACCGTGATGTCCCGGTTGCATCGTGGCCGCAAGCAGCTCCGTG
AGCTGCTCGCCGATGTCGCCCGAGACCGCGGGTTCCTCCGCGGTGCGCAAGCGCCCGAGGAGGTGTCGTCGTGA

Upstream 100 bases:

>100_bases
AACAACCTCGCGCGGTCCTGCTGTTGTTGGGAAAGACTTCGGGTGCGCACGCAGCCGAGAACGTGTCGGTCACAACCTCT
AGGATCAAGGGAGGACTCTG

Downstream 100 bases:

>100_bases
GTGACGAAGAGCGCTGGACCCCGCCGGTCGGCCCGGTCGACCCGGAGCATCCCGAATGCGCCGCCGTCATCGCGGAGGTG
TGGACGCTGCTAGACGGCGA

Product: ECF subfamily RNA polymerase sigma-24 factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MPTACLDRPVDLPGLFDGAATEGTVSTTMTDIDGAAAERQETDAELTARFERDAIPLLDQLYGGALRMTRNPADAEDLLQ
ETMVKAYAGFRSFREGTNLKAWLYRILTNTYINSYRKKQRQPSEYPTEEITDWQLAANAEHSSTGLRSAEVEALESLPDT
EIKAALQALPEDFRMAVYYADVEGFPYKEIAEIMDTPIGTVMSRLHRGRKQLRELLADVARDRGFLRGAQAPEEVSS

Sequences:

>Translated_237_residues
MPTACLDRPVDLPGLFDGAATEGTVSTTMTDIDGAAAERQETDAELTARFERDAIPLLDQLYGGALRMTRNPADAEDLLQ
ETMVKAYAGFRSFREGTNLKAWLYRILTNTYINSYRKKQRQPSEYPTEEITDWQLAANAEHSSTGLRSAEVEALESLPDT
EIKAALQALPEDFRMAVYYADVEGFPYKEIAEIMDTPIGTVMSRLHRGRKQLRELLADVARDRGFLRGAQAPEEVSS
>Mature_236_residues
PTACLDRPVDLPGLFDGAATEGTVSTTMTDIDGAAAERQETDAELTARFERDAIPLLDQLYGGALRMTRNPADAEDLLQE
TMVKAYAGFRSFREGTNLKAWLYRILTNTYINSYRKKQRQPSEYPTEEITDWQLAANAEHSSTGLRSAEVEALESLPDTE
IKAALQALPEDFRMAVYYADVEGFPYKEIAEIMDTPIGTVMSRLHRGRKQLRELLADVARDRGFLRGAQAPEEVSS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=145, Percent_Identity=35.8620689655172, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 26458; Mature: 26327

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTACLDRPVDLPGLFDGAATEGTVSTTMTDIDGAAAERQETDAELTARFERDAIPLLDQ
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LYGGALRMTRNPADAEDLLQETMVKAYAGFRSFREGTNLKAWLYRILTNTYINSYRKKQR
HCCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
QPSEYPTEEITDWQLAANAEHSSTGLRSAEVEALESLPDTEIKAALQALPEDFRMAVYYA
CCCCCCHHHCCCHHHHCCCCHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCHHHEEEEEEE
DVEGFPYKEIAEIMDTPIGTVMSRLHRGRKQLRELLADVARDRGFLRGAQAPEEVSS
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCC
>Mature Secondary Structure 
PTACLDRPVDLPGLFDGAATEGTVSTTMTDIDGAAAERQETDAELTARFERDAIPLLDQ
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LYGGALRMTRNPADAEDLLQETMVKAYAGFRSFREGTNLKAWLYRILTNTYINSYRKKQR
HCCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
QPSEYPTEEITDWQLAANAEHSSTGLRSAEVEALESLPDTEIKAALQALPEDFRMAVYYA
CCCCCCHHHCCCHHHHCCCCHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCHHHEEEEEEE
DVEGFPYKEIAEIMDTPIGTVMSRLHRGRKQLRELLADVARDRGFLRGAQAPEEVSS
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]