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The map label for this gene is narH [H]

Identifier: 119867329

GI number: 119867329

Start: 1368364

End: 1370019

Strand: Reverse

Name: narH [H]

Synonym: Mkms_1279

Alternate gene names: 119867329

Gene position: 1370019-1368364 (Counterclockwise)

Preceding gene: 119867330

Following gene: 119867328

Centisome position: 23.88

GC content: 66.97

Gene sequence:

>1656_bases
ATGCGAGTCATGGCCCAGATGGCGATGGTGATGAACCTCGACAAGTGCATCGGGTGTCATACCTGCTCGGTCACCTGCAA
GCAGGCGTGGACCAACCGGCCCGGAACCGAGTACGTGTGGTTCAACAACGTGGAAACCCGCCCGGGACAGGGTTATCCGC
GCACCTACGAGGATCAGGAGCGGTGGCGCGGCGGGTGGGTCCGCGACCGCAGGGGCCGGTTACGGTTGCGCGACGGCGGG
CGGCTGAGCAAGCTGCTGCGGATCTTCGCCAACCCCAAGATGCCGAGCATCGACGACTACTACGAGCCGTGGACGTACGA
CTACGAGAACCTCACCTCGGCTCCGCTGGGCGACCAGATGCCGGTGGCGCCACCGCGCAGCCTGATCAGCGGTGAGCCGA
TGAAGATCTCGTGGTCGGCGAACTGGGACGACGACCTCGGCGGCTCCCCCGAGATCGTGCCCGGTGATCCGGTGCTGGCG
AAGGTCAGCGAACAGGTCCGGCTCGAGTTCGAGCAGACCTTCATGTTCTTCCTGCCCCGCATCTGCGAGCACTGCCTCAA
CCCGTCGTGTATGGCGTCGTGTCCGTCGGGTGCGATCTACAAACGCAGCGAGGACGGCATCGTTCTCGTCGACCAGGACC
GCTGCCGCGGCTGGCGGATGTGTGTGTCCGGATGCCCCTACAAGAAGATGTATTTCAACCACAAGACCGGCAAGGCCGAG
AAGTGCACGCTGTGCTACCCACGCATCGAGGTCGGGCTGCCCACCGTGTGTTCGGAGACCTGCGTCGGCCGGTTGCGTTA
TCTGGGTCTGGTGCTCTACGACGTCGACCGTGTGCTGGAGGCGGCATCGGTGGAGAACGACGCCGACCTCTACGAGGCGC
AGAAGGGTGTACTGCTCGATCCGCACGACCCGGAGGTGGTCGCCGGGGCGCGCGCCGAAGGGATCTCCGACGAATGGATC
GAGGCGGCCCAGCGGTCGCCGATCTACGCGCTCATCCACACCTACCGGGTGGCGCTGCCGCTGCACCCGGAATACCGCAC
CATGCCGATGGTCTGGTACATCCCGCCACTGTCGCCGATCGTCGACGCGGTCGCCCGCGACGGTCACGACGGCGAGGAAC
TGGGCAACCTGTTCGGTGCGCTGGAGGCGCTGCGCATCCCGATCGAGTACCTCGCCGGGCTGTTCACCGCCGGAGACACC
GCGGTCGTCGAGGACGTCCTGCGCCGGCTCGCGGCGATGCGCTCCTACATGCGCGACATCAACCTCGGCCGCGAGACCCA
GCCGCACATCCCCGAGTCGGTGGGTCTGACCGAAGAAGAGATGTACGACATGTACCGGCTGCTCGCGCTGGCGAAATACG
ATGAGCGGTACGTCATCCCGACGGCCTACGCGGCCAGCGCGATCCCGGGAGCCGAGGAGCCCGGCTGCTCGTTGTCGTTC
GAGGGCGGACCGGGAATGTACGAATCCGGCCCGTTCGGTGAGGCCAGCGGCGGCCCGGTGGCGGTGGCCGTCGAGACGTT
CCACGCGCTGCAACACCGGCAGACCAGCGACGGGATGGCCGCCAACGCCGACCGCCCGTCACGGGTGAACCTGCTCAACT
GGGACGGCCGTGGCACACCGCCGGGCATCTTCCCGGGCGACGGGGACCGGCGATGA

Upstream 100 bases:

>100_bases
ACGCGCAGAACGCCTTCGCGTTCAACTACCTCGGGCCGACCGGTAACCAACGCGACGAGGTGACTGTGGTGCGGCGGCGA
AGCCAGGAGGTGGTGTTCTG

Downstream 100 bases:

>100_bases
AGACCGCGAAGCTCTTCAAGCGGTCCAGGTGCGGACTCGAGGAGCGGCTCGTCTGGCAGTGCGCGTCACTGCTGCTGAGC
TATCCCGACCACAGCCGGTT

Product: respiratory nitrate reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MRVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRPGTEYVWFNNVETRPGQGYPRTYEDQERWRGGWVRDRRGRLRLRDGG
RLSKLLRIFANPKMPSIDDYYEPWTYDYENLTSAPLGDQMPVAPPRSLISGEPMKISWSANWDDDLGGSPEIVPGDPVLA
KVSEQVRLEFEQTFMFFLPRICEHCLNPSCMASCPSGAIYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKMYFNHKTGKAE
KCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDRVLEAASVENDADLYEAQKGVLLDPHDPEVVAGARAEGISDEWI
EAAQRSPIYALIHTYRVALPLHPEYRTMPMVWYIPPLSPIVDAVARDGHDGEELGNLFGALEALRIPIEYLAGLFTAGDT
AVVEDVLRRLAAMRSYMRDINLGRETQPHIPESVGLTEEEMYDMYRLLALAKYDERYVIPTAYAASAIPGAEEPGCSLSF
EGGPGMYESGPFGEASGGPVAVAVETFHALQHRQTSDGMAANADRPSRVNLLNWDGRGTPPGIFPGDGDRR

Sequences:

>Translated_551_residues
MRVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRPGTEYVWFNNVETRPGQGYPRTYEDQERWRGGWVRDRRGRLRLRDGG
RLSKLLRIFANPKMPSIDDYYEPWTYDYENLTSAPLGDQMPVAPPRSLISGEPMKISWSANWDDDLGGSPEIVPGDPVLA
KVSEQVRLEFEQTFMFFLPRICEHCLNPSCMASCPSGAIYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKMYFNHKTGKAE
KCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDRVLEAASVENDADLYEAQKGVLLDPHDPEVVAGARAEGISDEWI
EAAQRSPIYALIHTYRVALPLHPEYRTMPMVWYIPPLSPIVDAVARDGHDGEELGNLFGALEALRIPIEYLAGLFTAGDT
AVVEDVLRRLAAMRSYMRDINLGRETQPHIPESVGLTEEEMYDMYRLLALAKYDERYVIPTAYAASAIPGAEEPGCSLSF
EGGPGMYESGPFGEASGGPVAVAVETFHALQHRQTSDGMAANADRPSRVNLLNWDGRGTPPGIFPGDGDRR
>Mature_551_residues
MRVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRPGTEYVWFNNVETRPGQGYPRTYEDQERWRGGWVRDRRGRLRLRDGG
RLSKLLRIFANPKMPSIDDYYEPWTYDYENLTSAPLGDQMPVAPPRSLISGEPMKISWSANWDDDLGGSPEIVPGDPVLA
KVSEQVRLEFEQTFMFFLPRICEHCLNPSCMASCPSGAIYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKMYFNHKTGKAE
KCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDRVLEAASVENDADLYEAQKGVLLDPHDPEVVAGARAEGISDEWI
EAAQRSPIYALIHTYRVALPLHPEYRTMPMVWYIPPLSPIVDAVARDGHDGEELGNLFGALEALRIPIEYLAGLFTAGDT
AVVEDVLRRLAAMRSYMRDINLGRETQPHIPESVGLTEEEMYDMYRLLALAKYDERYVIPTAYAASAIPGAEEPGCSLSF
EGGPGMYESGPFGEASGGPVAVAVETFHALQHRQTSDGMAANADRPSRVNLLNWDGRGTPPGIFPGDGDRR

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=485, Percent_Identity=56.701030927835, Blast_Score=584, Evalue=1e-168,
Organism=Escherichia coli, GI1787478, Length=490, Percent_Identity=55.7142857142857, Blast_Score=576, Evalue=1e-165,
Organism=Escherichia coli, GI1787872, Length=101, Percent_Identity=40.5940594059406, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=40.5940594059406, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI226510944, Length=92, Percent_Identity=36.9565217391304, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787749, Length=80, Percent_Identity=36.25, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789370, Length=102, Percent_Identity=34.3137254901961, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI2367345, Length=137, Percent_Identity=28.4671532846715, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790326, Length=80, Percent_Identity=35, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 61577; Mature: 61577

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRPGTEYVWFNNVETRPGQGYPRTYEDQE
CHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHH
RWRGGWVRDRRGRLRLRDGGRLSKLLRIFANPKMPSIDDYYEPWTYDYENLTSAPLGDQM
HHCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
PVAPPRSLISGEPMKISWSANWDDDLGGSPEIVPGDPVLAKVSEQVRLEFEQTFMFFLPR
CCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ICEHCLNPSCMASCPSGAIYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKMYFNHKTGKAE
HHHHHCCCHHHHCCCCCCEEEECCCCEEEEECHHCCHHHHHHCCCCCHHHEEECCCCCCC
KCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDRVLEAASVENDADLYEAQKGVLLD
EEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEC
PHDPEVVAGARAEGISDEWIEAAQRSPIYALIHTYRVALPLHPEYRTMPMVWYIPPLSPI
CCCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEHHHEEEECCCCCCCCCEEEEECCHHHH
VDAVARDGHDGEELGNLFGALEALRIPIEYLAGLFTAGDTAVVEDVLRRLAAMRSYMRDI
HHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NLGRETQPHIPESVGLTEEEMYDMYRLLALAKYDERYVIPTAYAASAIPGAEEPGCSLSF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCCCCCCEEEE
EGGPGMYESGPFGEASGGPVAVAVETFHALQHRQTSDGMAANADRPSRVNLLNWDGRGTP
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCC
PGIFPGDGDRR
CCCCCCCCCCC
>Mature Secondary Structure
MRVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRPGTEYVWFNNVETRPGQGYPRTYEDQE
CHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHH
RWRGGWVRDRRGRLRLRDGGRLSKLLRIFANPKMPSIDDYYEPWTYDYENLTSAPLGDQM
HHCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
PVAPPRSLISGEPMKISWSANWDDDLGGSPEIVPGDPVLAKVSEQVRLEFEQTFMFFLPR
CCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ICEHCLNPSCMASCPSGAIYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKMYFNHKTGKAE
HHHHHCCCHHHHCCCCCCEEEECCCCEEEEECHHCCHHHHHHCCCCCHHHEEECCCCCCC
KCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDRVLEAASVENDADLYEAQKGVLLD
EEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEC
PHDPEVVAGARAEGISDEWIEAAQRSPIYALIHTYRVALPLHPEYRTMPMVWYIPPLSPI
CCCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEHHHEEEECCCCCCCCCEEEEECCHHHH
VDAVARDGHDGEELGNLFGALEALRIPIEYLAGLFTAGDTAVVEDVLRRLAAMRSYMRDI
HHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NLGRETQPHIPESVGLTEEEMYDMYRLLALAKYDERYVIPTAYAASAIPGAEEPGCSLSF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCCCCCCEEEE
EGGPGMYESGPFGEASGGPVAVAVETFHALQHRQTSDGMAANADRPSRVNLLNWDGRGTP
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCC
PGIFPGDGDRR
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]