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The map label for this gene is punA [H]

Identifier: 119867311

GI number: 119867311

Start: 1351274

End: 1352068

Strand: Reverse

Name: punA [H]

Synonym: Mkms_1261

Alternate gene names: 119867311

Gene position: 1352068-1351274 (Counterclockwise)

Preceding gene: 119867314

Following gene: 119867309

Centisome position: 23.57

GC content: 74.34

Gene sequence:

>795_bases
GTGACCAGCCCAGCGGAGGTGGCCCGGCAGGCCGCCGCGGTGATCCGCGACCGCACCGGAGCCGACGAACACGACGTCGC
GGTCGTGCTCGGATCGGGCTGGGCGCCCGCGGCCGACGCCCTCGGCACCGCCACCGCGTCCGTGCCGATGGCCGAGTTGC
CCGGGTTCACGCCGCCGACGGCCGCGGGCCACGGCGGGGTCGCGCTGTCCGTTCCGATCGGCGGGCACCGCGTGCTGGTC
CTCGTCGGCCGCATCCACGCCTACGAGGGCCACGATCTGCGCCACGTCGTGCACCCGGTGCGGGCGGCGTGCGCGGCGGG
CGTGCGCACCGTCGTGCTGACCAACGCCGCCGGCGGGCTCCGCGAGGACTACACCGTGGGTCAGCCGGTGGTGATCAGCG
ACCACCTCAACCTCACCGCCCGCTCCCCGCTGGTGGGGGCGCAATTCGTCGATCTGGTCGACGCGTACGCACCGCGGCTG
CGAACACTGGCCAGGACGATCGACCCGACCCTGGCCGAAGGCGTCTACGCCGGACTGCCGGGGCCGCACTACGAGACCCC
GGCCGAGATCCGGATGCTGCGCACGCTCGGCGCGGATCTGGTCGGCATGTCGACAGTGCACGAGACGATCGCCGCGCGCG
CGGCGGGCGCCGAGGTGCTGGCGATCTCGCTGGTGACCAATCTGGCCGCCGGCATGACCGGGCAGCCGCTCTCGCACCAG
GAGGTGCTGGAGGCGGGCCGTCAGTCCGCGACGCGGATGGGTGCTCTGCTGGCCGGCGTCGTCGCATCGCTCTGA

Upstream 100 bases:

>100_bases
TGGACGGCGTCTTCGACGCTCGTCGACGCGGTGGCGGAGGGCATTGACGCAGTATCTCACTGCACGAATCGCGCGGCTTC
GACAGCTAAGCTCGCACACC

Downstream 100 bases:

>100_bases
TCGCCGGCGTGACGGCTCAGCCGCCGGCTCGCCCACACGACGAAGCGGGCCAGCAGCGGTGAGGCGAACAGCATGAGCAG
CACCCGCAAGACCTGCGCGG

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: PNP; Inosine phosphorylase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTSPAEVARQAAAVIRDRTGADEHDVAVVLGSGWAPAADALGTATASVPMAELPGFTPPTAAGHGGVALSVPIGGHRVLV
LVGRIHAYEGHDLRHVVHPVRAACAAGVRTVVLTNAAGGLREDYTVGQPVVISDHLNLTARSPLVGAQFVDLVDAYAPRL
RTLARTIDPTLAEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVHETIAARAAGAEVLAISLVTNLAAGMTGQPLSHQ
EVLEAGRQSATRMGALLAGVVASL

Sequences:

>Translated_264_residues
MTSPAEVARQAAAVIRDRTGADEHDVAVVLGSGWAPAADALGTATASVPMAELPGFTPPTAAGHGGVALSVPIGGHRVLV
LVGRIHAYEGHDLRHVVHPVRAACAAGVRTVVLTNAAGGLREDYTVGQPVVISDHLNLTARSPLVGAQFVDLVDAYAPRL
RTLARTIDPTLAEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVHETIAARAAGAEVLAISLVTNLAAGMTGQPLSHQ
EVLEAGRQSATRMGALLAGVVASL
>Mature_263_residues
TSPAEVARQAAAVIRDRTGADEHDVAVVLGSGWAPAADALGTATASVPMAELPGFTPPTAAGHGGVALSVPIGGHRVLVL
VGRIHAYEGHDLRHVVHPVRAACAAGVRTVVLTNAAGGLREDYTVGQPVVISDHLNLTARSPLVGAQFVDLVDAYAPRLR
TLARTIDPTLAEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVHETIAARAAGAEVLAISLVTNLAAGMTGQPLSHQE
VLEAGRQSATRMGALLAGVVASL

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=257, Percent_Identity=37.3540856031128, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI1788746, Length=250, Percent_Identity=35.6, Blast_Score=144, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17541190, Length=272, Percent_Identity=37.8676470588235, Blast_Score=167, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6323238, Length=283, Percent_Identity=34.6289752650177, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24656090, Length=272, Percent_Identity=34.1911764705882, Blast_Score=131, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24656093, Length=272, Percent_Identity=34.1911764705882, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45552885, Length=270, Percent_Identity=34.0740740740741, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45552887, Length=281, Percent_Identity=32.0284697508897, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24762376, Length=222, Percent_Identity=28.8288288288288, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011269
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 26999; Mature: 26868

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPAEVARQAAAVIRDRTGADEHDVAVVLGSGWAPAADALGTATASVPMAELPGFTPPT
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHCCCCCCCC
AAGHGGVALSVPIGGHRVLVLVGRIHAYEGHDLRHVVHPVRAACAAGVRTVVLTNAAGGL
CCCCCCEEEEECCCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCC
REDYTVGQPVVISDHLNLTARSPLVGAQFVDLVDAYAPRLRTLARTIDPTLAEGVYAGLP
CCCCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCC
GPHYETPAEIRMLRTLGADLVGMSTVHETIAARAAGAEVLAISLVTNLAAGMTGQPLSHQ
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHH
EVLEAGRQSATRMGALLAGVVASL
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSPAEVARQAAAVIRDRTGADEHDVAVVLGSGWAPAADALGTATASVPMAELPGFTPPT
CCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHCCCCCCCC
AAGHGGVALSVPIGGHRVLVLVGRIHAYEGHDLRHVVHPVRAACAAGVRTVVLTNAAGGL
CCCCCCEEEEECCCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCC
REDYTVGQPVVISDHLNLTARSPLVGAQFVDLVDAYAPRLRTLARTIDPTLAEGVYAGLP
CCCCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCC
GPHYETPAEIRMLRTLGADLVGMSTVHETIAARAAGAEVLAISLVTNLAAGMTGQPLSHQ
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHH
EVLEAGRQSATRMGALLAGVVASL
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]