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The map label for this gene is dacB [H]

Identifier: 119867283

GI number: 119867283

Start: 1319260

End: 1320492

Strand: Reverse

Name: dacB [H]

Synonym: Mkms_1233

Alternate gene names: 119867283

Gene position: 1320492-1319260 (Counterclockwise)

Preceding gene: 119867284

Following gene: 119867281

Centisome position: 23.02

GC content: 73.15

Gene sequence:

>1233_bases
ATGGCCGCCTCACGGAATCTCTCGACGCGCGTCGCGGCGCTGGCCGCGGCGGTGTCGGTGCTGATCGCGCCCGCACTGAT
CGCGGCGCCGGGGGCCGCGGCGCAGCCCGCACCCCAGTCCACCACCTGCCCGTACCGCGTGACCACGCCGCCGGCCGTCG
ACGCCTCCGAAGTGCCCAAGCCCGGACAGGATCCGCCCGTCGCGCTGCCGGTGCCCGCCACCCCGCTCGGCGGTGACGCG
CTGTCCGGATGCGGCATCATCACCGCACCGGGCGCCCCGCCCGCGCCCGCCGACGTGTCCGCCGAGGCGTGGCTGGTGGC
CGACATGGACACCGGTGAGGTGATCGCCGCGCGCGACCCACACGGTCGGCACCGCCCGGCCAGCGTGATCAAGGTGCTGA
TCGCGATCCAGTCGCTCAAGGAGCTCAACCTCGCCAAGGCCGTGCCGGGCACCCCCGAGGACGGCGCCGCCGAGGGCACC
AGCGTCGGCGTCGACGTCGGCGGCCTCTACACCGTCAACGATCTGCTGCACGGGCTGCTGATGTACTCCGGCAACGACGC
CGCGCACGCCCTGGCGCGGCAACTCGGTGGAATGGACACCGCGCTGCTCAAGCTCAACCAGCTGGCCGGAAAGCTCGGCG
GCCGCGACACCCGGGTGGCCACCCCGTCGGGCCTCGACGGGCCGGGCATGAGCACCTCGGCCTACGACATCGCGCTGTTC
TACCGGTACGCCTGGCGCAACCCGACTTTCACCGAGATCGTCGCCACCCGCACCTACGACTTCCCGGGCCGGGACGGCAA
CCCGCCCTACCCGATCGAGAACGACGTCAAGCTGCTCTACAACTATCCGGGCGCCCTCGGCGGTAAGACCGGCTTCACCG
ACGACGCCGGACAGACCTTCGTCGGTGGCGCCGAGCGCGACGGCCGCCGCCTGGTGGCGGTGCTGCTGCGCGGCACCCGG
CAGCCGATCGCCCCGTGGGAGCAGGCCGCCCGGCTGCTCGACTACGGCTTCGGGCTGCCGCAGGGCACCAAGGTGGGATC
GCTGATCGAACCGGATCCGTCGCTGGACCCGAGTCCGCAGACCGCCGAGACCGAGGCGGCCGCGCAGGCGGCTGCGGTGC
TGCCCGCCGCCGACGCCATGCCCGTGCGGGTCGGGGTGGGCGTGATCGGCAGCATCGTCGTGTTCGCGTTGATGATGGCG
GCCCGCTCGCTCAACCGGCGTCCGCAGAACTGA

Upstream 100 bases:

>100_bases
AGTCTCGGCGCGACGCGGCGCCCCCGCGAGTCACACGAACCACATCCGTCACAGCGAGCCTCCGGGCAAACGGCCCGCGC
ATCACTTAATCTCCGCGGTG

Downstream 100 bases:

>100_bases
CCCAGCGGCGCAGCAGCCCCTCGGCCTCCGCGCACAGTCGCGCGATCAGGTCGGCCGCCGGCTCCGCCTGCGTCACCATG
CCCACCCCCTGGCCCGCGTT

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MAASRNLSTRVAALAAAVSVLIAPALIAAPGAAAQPAPQSTTCPYRVTTPPAVDASEVPKPGQDPPVALPVPATPLGGDA
LSGCGIITAPGAPPAPADVSAEAWLVADMDTGEVIAARDPHGRHRPASVIKVLIAIQSLKELNLAKAVPGTPEDGAAEGT
SVGVDVGGLYTVNDLLHGLLMYSGNDAAHALARQLGGMDTALLKLNQLAGKLGGRDTRVATPSGLDGPGMSTSAYDIALF
YRYAWRNPTFTEIVATRTYDFPGRDGNPPYPIENDVKLLYNYPGALGGKTGFTDDAGQTFVGGAERDGRRLVAVLLRGTR
QPIAPWEQAARLLDYGFGLPQGTKVGSLIEPDPSLDPSPQTAETEAAAQAAAVLPAADAMPVRVGVGVIGSIVVFALMMA
ARSLNRRPQN

Sequences:

>Translated_410_residues
MAASRNLSTRVAALAAAVSVLIAPALIAAPGAAAQPAPQSTTCPYRVTTPPAVDASEVPKPGQDPPVALPVPATPLGGDA
LSGCGIITAPGAPPAPADVSAEAWLVADMDTGEVIAARDPHGRHRPASVIKVLIAIQSLKELNLAKAVPGTPEDGAAEGT
SVGVDVGGLYTVNDLLHGLLMYSGNDAAHALARQLGGMDTALLKLNQLAGKLGGRDTRVATPSGLDGPGMSTSAYDIALF
YRYAWRNPTFTEIVATRTYDFPGRDGNPPYPIENDVKLLYNYPGALGGKTGFTDDAGQTFVGGAERDGRRLVAVLLRGTR
QPIAPWEQAARLLDYGFGLPQGTKVGSLIEPDPSLDPSPQTAETEAAAQAAAVLPAADAMPVRVGVGVIGSIVVFALMMA
ARSLNRRPQN
>Mature_409_residues
AASRNLSTRVAALAAAVSVLIAPALIAAPGAAAQPAPQSTTCPYRVTTPPAVDASEVPKPGQDPPVALPVPATPLGGDAL
SGCGIITAPGAPPAPADVSAEAWLVADMDTGEVIAARDPHGRHRPASVIKVLIAIQSLKELNLAKAVPGTPEDGAAEGTS
VGVDVGGLYTVNDLLHGLLMYSGNDAAHALARQLGGMDTALLKLNQLAGKLGGRDTRVATPSGLDGPGMSTSAYDIALFY
RYAWRNPTFTEIVATRTYDFPGRDGNPPYPIENDVKLLYNYPGALGGKTGFTDDAGQTFVGGAERDGRRLVAVLLRGTRQ
PIAPWEQAARLLDYGFGLPQGTKVGSLIEPDPSLDPSPQTAETEAAAQAAAVLPAADAMPVRVGVGVIGSIVVFALMMAA
RSLNRRPQN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=275, Percent_Identity=29.4545454545455, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1787062, Length=255, Percent_Identity=30.9803921568627, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42137; Mature: 42006

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASRNLSTRVAALAAAVSVLIAPALIAAPGAAAQPAPQSTTCPYRVTTPPAVDASEVPK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCHHHCCC
PGQDPPVALPVPATPLGGDALSGCGIITAPGAPPAPADVSAEAWLVADMDTGEVIAARDP
CCCCCCEEECCCCCCCCCCHHCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECCC
HGRHRPASVIKVLIAIQSLKELNLAKAVPGTPEDGAAEGTSVGVDVGGLYTVNDLLHGLL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEHHHHHHHHH
MYSGNDAAHALARQLGGMDTALLKLNQLAGKLGGRDTRVATPSGLDGPGMSTSAYDIALF
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHEEE
YRYAWRNPTFTEIVATRTYDFPGRDGNPPYPIENDVKLLYNYPGALGGKTGFTDDAGQTF
EHHHCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCE
VGGAERDGRRLVAVLLRGTRQPIAPWEQAARLLDYGFGLPQGTKVGSLIEPDPSLDPSPQ
ECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
TAETEAAAQAAAVLPAADAMPVRVGVGVIGSIVVFALMMAARSLNRRPQN
CCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AASRNLSTRVAALAAAVSVLIAPALIAAPGAAAQPAPQSTTCPYRVTTPPAVDASEVPK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCHHHCCC
PGQDPPVALPVPATPLGGDALSGCGIITAPGAPPAPADVSAEAWLVADMDTGEVIAARDP
CCCCCCEEECCCCCCCCCCHHCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECCC
HGRHRPASVIKVLIAIQSLKELNLAKAVPGTPEDGAAEGTSVGVDVGGLYTVNDLLHGLL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEHHHHHHHHH
MYSGNDAAHALARQLGGMDTALLKLNQLAGKLGGRDTRVATPSGLDGPGMSTSAYDIALF
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHEEE
YRYAWRNPTFTEIVATRTYDFPGRDGNPPYPIENDVKLLYNYPGALGGKTGFTDDAGQTF
EHHHCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCE
VGGAERDGRRLVAVLLRGTRQPIAPWEQAARLLDYGFGLPQGTKVGSLIEPDPSLDPSPQ
ECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
TAETEAAAQAAAVLPAADAMPVRVGVGVIGSIVVFALMMAARSLNRRPQN
CCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]