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The map label for this gene is folD

Identifier: 119867271

GI number: 119867271

Start: 1307185

End: 1308051

Strand: Direct

Name: folD

Synonym: Mkms_1221

Alternate gene names: 119867271

Gene position: 1307185-1308051 (Clockwise)

Preceding gene: 119867264

Following gene: 119867272

Centisome position: 22.78

GC content: 69.2

Gene sequence:

>867_bases
ATGTCTGGCGTGGGTGCGATAACGCTCGATGGGAAAGCCACGCGGGACGAGATCTTCGTCGACCTCAAGGAACGGGTGGC
GGCGCTCACCGAACAGGGGCGCACACCGGGACTCGGCACCGTGCTGGTCGGTGACGATCCGGGTTCTCAGGCTTACGTAC
GCGGTAAGCACTCCGACTGCGCGAAGGTCGGCATCAATTCGATCCGCCGCGACCTGCCCGCCGACATCAGCCAGGCCGAA
CTCGACGCGACGATCGACGAGCTCAACGCCAACCCCGAATGCACCGGCTACATCGTGCAGCTGCCACTGCCCAAGCACCT
GGACGAGAACGCGGCGCTCGAACGCATCGACCCGGGGAAGGACGCCGACGGTCTGCATCCGACCAACCTGGGCCGGCTGG
TGCTCAACGAACCCGCACCTCTGCCGTGCACGCCGCGGGGGATCGTGCACCTGCTGCGCCGGTACGAGGTCGAGATCGCA
GGCGCGCACGTGGTGGTGATCGGCCGCGGCGTGACGGTCGGGCGGCCACTCGGGCTGCTGCTGACCCGCCGCTCGGAGAA
CGCCACAGTCACCCTGTGCCACACCGCAACCCGGCATCTGCCGCAGTTCACCCGCGAGGCCGACATCATCGTCGCCGCGG
CGGGGGTGCCGCACATGGTGACCGCGGAGATGGTGCGACCGGGTGCGGCGGTGATCGATGTCGGGGTCAGCCGCGACGAC
AACGGCAAGCTCGTCGGCGACGTCGCGCCCGACGTGTGGGAGGTGGCCGGACACGTGTCGCCCAACCCGGGTGGCGTGGG
TCCGCTGACGCGGGCGTTCCTGCTGACAAACGTCGTCGAACGCGCCGAGGCTCTCGCGCGCGGGTGA

Upstream 100 bases:

>100_bases
AAGGTGGCGGCCTTGATGATGCGGTTGCGCAGGGTCACCGGGCCGAGCTTGGCCGGGGTGAACACATCGGGCTGGGTGTC
CATACCGGCAGCCTGCCACA

Downstream 100 bases:

>100_bases
CCGTCAAGGACTTCGCCCGCAAGGTCTTCGCGGGGCAGTGGCCGATCCTGGTGGTCGGCCTGATCTTCGTGGCCGCGTTC
GCCCTTGTGATCGCCGGTTA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSGVGAITLDGKATRDEIFVDLKERVAALTEQGRTPGLGTVLVGDDPGSQAYVRGKHSDCAKVGINSIRRDLPADISQAE
LDATIDELNANPECTGYIVQLPLPKHLDENAALERIDPGKDADGLHPTNLGRLVLNEPAPLPCTPRGIVHLLRRYEVEIA
GAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTATRHLPQFTREADIIVAAAGVPHMVTAEMVRPGAAVIDVGVSRDD
NGKLVGDVAPDVWEVAGHVSPNPGGVGPLTRAFLLTNVVERAEALARG

Sequences:

>Translated_288_residues
MSGVGAITLDGKATRDEIFVDLKERVAALTEQGRTPGLGTVLVGDDPGSQAYVRGKHSDCAKVGINSIRRDLPADISQAE
LDATIDELNANPECTGYIVQLPLPKHLDENAALERIDPGKDADGLHPTNLGRLVLNEPAPLPCTPRGIVHLLRRYEVEIA
GAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTATRHLPQFTREADIIVAAAGVPHMVTAEMVRPGAAVIDVGVSRDD
NGKLVGDVAPDVWEVAGHVSPNPGGVGPLTRAFLLTNVVERAEALARG
>Mature_287_residues
SGVGAITLDGKATRDEIFVDLKERVAALTEQGRTPGLGTVLVGDDPGSQAYVRGKHSDCAKVGINSIRRDLPADISQAEL
DATIDELNANPECTGYIVQLPLPKHLDENAALERIDPGKDADGLHPTNLGRLVLNEPAPLPCTPRGIVHLLRRYEVEIAG
AHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTATRHLPQFTREADIIVAAAGVPHMVTAEMVRPGAAVIDVGVSRDDN
GKLVGDVAPDVWEVAGHVSPNPGGVGPLTRAFLLTNVVERAEALARG

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=40.3389830508475, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI94721354, Length=297, Percent_Identity=39.3939393939394, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=40.4844290657439, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI36796743, Length=229, Percent_Identity=27.0742358078603, Blast_Score=71, Evalue=9e-13,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=43.7275985663082, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17568735, Length=257, Percent_Identity=38.5214007782101, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319558, Length=253, Percent_Identity=40.7114624505929, Blast_Score=188, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6321643, Length=292, Percent_Identity=38.013698630137, Blast_Score=172, Evalue=5e-44,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=41.7808219178082, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=41.7808219178082, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=42.4137931034483, Blast_Score=200, Evalue=7e-52,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=42.4137931034483, Blast_Score=200, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=42.4137931034483, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=42.4137931034483, Blast_Score=200, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_MYCSJ (A3PVV6)

Other databases:

- EMBL:   CP000580
- RefSeq:   YP_001069524.1
- ProteinModelPortal:   A3PVV6
- SMR:   A3PVV6
- STRING:   A3PVV6
- EnsemblBacteria:   EBMYCT00000049515
- GeneID:   4876967
- GenomeReviews:   CP000580_GR
- KEGG:   mjl:Mjls_1231
- eggNOG:   COG0190
- GeneTree:   EBGT00050000016741
- HOGENOM:   HBG328751
- OMA:   LLEHYHI
- ProtClustDB:   PRK14193
- BioCyc:   MSP164757:MJLS_1231-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30414; Mature: 30283

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGVGAITLDGKATRDEIFVDLKERVAALTEQGRTPGLGTVLVGDDPGSQAYVRGKHSDC
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCH
AKVGINSIRRDLPADISQAELDATIDELNANPECTGYIVQLPLPKHLDENAALERIDPGK
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHCCCCCC
DADGLHPTNLGRLVLNEPAPLPCTPRGIVHLLRRYEVEIAGAHVVVIGRGVTVGRPLGLL
CCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCEEECCEEEEEEECCCCCCCCCEEE
LTRRSENATVTLCHTATRHLPQFTREADIIVAAAGVPHMVTAEMVRPGAAVIDVGVSRDD
EEECCCCCEEEEEEHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCEEEEEECCCCC
NGKLVGDVAPDVWEVAGHVSPNPGGVGPLTRAFLLTNVVERAEALARG
CCCEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGVGAITLDGKATRDEIFVDLKERVAALTEQGRTPGLGTVLVGDDPGSQAYVRGKHSDC
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCH
AKVGINSIRRDLPADISQAELDATIDELNANPECTGYIVQLPLPKHLDENAALERIDPGK
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHCCCCCC
DADGLHPTNLGRLVLNEPAPLPCTPRGIVHLLRRYEVEIAGAHVVVIGRGVTVGRPLGLL
CCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCEEECCEEEEEEECCCCCCCCCEEE
LTRRSENATVTLCHTATRHLPQFTREADIIVAAAGVPHMVTAEMVRPGAAVIDVGVSRDD
EEECCCCCEEEEEEHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCEEEEEECCCCC
NGKLVGDVAPDVWEVAGHVSPNPGGVGPLTRAFLLTNVVERAEALARG
CCCEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA