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The map label for this gene is baiCD [H]

Identifier: 119867270

GI number: 119867270

Start: 1305962

End: 1307167

Strand: Reverse

Name: baiCD [H]

Synonym: Mkms_1220

Alternate gene names: 119867270

Gene position: 1307167-1305962 (Counterclockwise)

Preceding gene: 119867273

Following gene: 119867269

Centisome position: 22.78

GC content: 68.74

Gene sequence:

>1206_bases
ATGGACACCCAGCCCGATGTGTTCACCCCGGCCAAGCTCGGCCCGGTGACCCTGCGCAACCGCATCATCAAGGCCGCCAC
CTTCGAGGCGGCGACGCCGAACGCGCTGGTCACCGACGACCTCATCCACTATCACCGGTTGCCTGCCGCCGGCGGCGTCG
GAATGACCACGGTCGCCTACTGCGCGGTGGCACCCGGCGGGCGCACCGACGGCTGGCAGATCTGGATGCGGCCCGAGGCG
ATTCCCGGCCTGCGCCGCCTCACCGACGCGGTGCACGCCGAAGGTGCGGCGATCAGCGCGCAGATCGGGCACGCAGGCCC
GGTCGCCAACTCCCGCACCAACAAGGCCAAGGCGCTCGCGCCGGTGCGGTTCTTCAACCCGCTGTCGATGCGGTTCGCCA
AGAAGGCCACCCGCGAGGACATCGCCGAGGTCACCGCCGCGCACGCCAACGCCGCGCGGCTGGCGATCGACTCCGGTTTC
GACGCGGTCGAGGTGCACCTGGGCCACAACTACCTGGCCAGCTCGTTCCTGAGCCCGCTGATCAACAAGCGCAGGGACGA
GTTCGGTGGGTCACTGGAGAACCGGGCCAAGGTCGCGCGCGGGGTCGTGCGCGCGGTGCGCGACGCGGTCGGCAACCAGA
TCGCGGTGACCGCGAAACTCAACATGACCGACGGTGTGCGCGCCGGGATCCCGCTCGAGGAGTCGCTGCAGACCGCCAAG
TGGCTCGAGGAGGACGGCGGCCTCGACGCCCTGGAACTCACCGCGGGCAGTTCGCTGGTGAACCCGCTGTACCTGTTCCA
CGGCGACGCGCCTGTCAAGGAGTTCGCGGGTGCGTTCAAGCCGCCGCTGAGCTGGGGCATCCGGATGACCGGCAAGAAGT
TCTTCCGCGAGTACCCCTACCGCGAGGCCTTCCTGCTGGACAAGGCCAGGAAGTTCCGCGCCGAACTCACCATGCCGCTG
ATCCTGTTGGGTGGCATCACCAACCGCGAGACCATGGACCTGGCGATGGCCGAGGGATTCGAGTTCGTCGCGATGGGCCG
CGCCCTGCTCGCCGAGCCCGACCTCATCAACCGGATCAAGGAGGACGGCGGTGCGCACAGCGTCCGATCGCTGTGCACGC
ACTGCAACAAGTGCATGGCGACGATCTACAGCCACACCCACTGTGTGGTGACCGGCGCGCCGGATTCGCTGCCCGTGCTG
GGCTGA

Upstream 100 bases:

>100_bases
GCCGCCACCCGTTCCTTGAGGTCGACGAAGATCTCGTCCCGCGTGGCTTTCCCATCGAGCGTTATCGCACCCACGCCAGA
CATTGTGGCAGGCTGCCGGT

Downstream 100 bases:

>100_bases
CACCGTGGAGGCGGCCCGCGACGCCATGGTCACCTACCGCTACGTGCGGGTGGGCCTGGTCGCGCTGGTCGTCTTCCTGC
TGGTCTCCGTCGGCCTCACC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Bile acid-inducible operon protein C; Bile acid-inducible operon protein D [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MDTQPDVFTPAKLGPVTLRNRIIKAATFEAATPNALVTDDLIHYHRLPAAGGVGMTTVAYCAVAPGGRTDGWQIWMRPEA
IPGLRRLTDAVHAEGAAISAQIGHAGPVANSRTNKAKALAPVRFFNPLSMRFAKKATREDIAEVTAAHANAARLAIDSGF
DAVEVHLGHNYLASSFLSPLINKRRDEFGGSLENRAKVARGVVRAVRDAVGNQIAVTAKLNMTDGVRAGIPLEESLQTAK
WLEEDGGLDALELTAGSSLVNPLYLFHGDAPVKEFAGAFKPPLSWGIRMTGKKFFREYPYREAFLLDKARKFRAELTMPL
ILLGGITNRETMDLAMAEGFEFVAMGRALLAEPDLINRIKEDGGAHSVRSLCTHCNKCMATIYSHTHCVVTGAPDSLPVL
G

Sequences:

>Translated_401_residues
MDTQPDVFTPAKLGPVTLRNRIIKAATFEAATPNALVTDDLIHYHRLPAAGGVGMTTVAYCAVAPGGRTDGWQIWMRPEA
IPGLRRLTDAVHAEGAAISAQIGHAGPVANSRTNKAKALAPVRFFNPLSMRFAKKATREDIAEVTAAHANAARLAIDSGF
DAVEVHLGHNYLASSFLSPLINKRRDEFGGSLENRAKVARGVVRAVRDAVGNQIAVTAKLNMTDGVRAGIPLEESLQTAK
WLEEDGGLDALELTAGSSLVNPLYLFHGDAPVKEFAGAFKPPLSWGIRMTGKKFFREYPYREAFLLDKARKFRAELTMPL
ILLGGITNRETMDLAMAEGFEFVAMGRALLAEPDLINRIKEDGGAHSVRSLCTHCNKCMATIYSHTHCVVTGAPDSLPVL
G
>Mature_401_residues
MDTQPDVFTPAKLGPVTLRNRIIKAATFEAATPNALVTDDLIHYHRLPAAGGVGMTTVAYCAVAPGGRTDGWQIWMRPEA
IPGLRRLTDAVHAEGAAISAQIGHAGPVANSRTNKAKALAPVRFFNPLSMRFAKKATREDIAEVTAAHANAARLAIDSGF
DAVEVHLGHNYLASSFLSPLINKRRDEFGGSLENRAKVARGVVRAVRDAVGNQIAVTAKLNMTDGVRAGIPLEESLQTAK
WLEEDGGLDALELTAGSSLVNPLYLFHGDAPVKEFAGAFKPPLSWGIRMTGKKFFREYPYREAFLLDKARKFRAELTMPL
ILLGGITNRETMDLAMAEGFEFVAMGRALLAEPDLINRIKEDGGAHSVRSLCTHCNKCMATIYSHTHCVVTGAPDSLPVL
G

Specific function: Catalyzes The NADP-Dependent Reduction Of 2,4-Dienoyl- CoA To Yield Trans-2- Enoyl-CoA. [C]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=399, Percent_Identity=27.0676691729323, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1787939, Length=378, Percent_Identity=26.984126984127, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=232, Percent_Identity=32.3275862068966, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=402, Percent_Identity=23.6318407960199, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=372, Percent_Identity=26.3440860215054, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=377, Percent_Identity=26.2599469496021, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=370, Percent_Identity=26.4864864864865, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=246, Percent_Identity=27.6422764227642, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=372, Percent_Identity=23.6559139784946, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321973, Length=223, Percent_Identity=29.1479820627803, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=225, Percent_Identity=27.5555555555556, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 43263; Mature: 43263

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTQPDVFTPAKLGPVTLRNRIIKAATFEAATPNALVTDDLIHYHRLPAAGGVGMTTVAY
CCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
CAVAPGGRTDGWQIWMRPEAIPGLRRLTDAVHAEGAAISAQIGHAGPVANSRTNKAKALA
HHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHC
PVRFFNPLSMRFAKKATREDIAEVTAAHANAARLAIDSGFDAVEVHLGHNYLASSFLSPL
CHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCEEEEEECCHHHHHHHHHHH
INKRRDEFGGSLENRAKVARGVVRAVRDAVGNQIAVTAKLNMTDGVRAGIPLEESLQTAK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHH
WLEEDGGLDALELTAGSSLVNPLYLFHGDAPVKEFAGAFKPPLSWGIRMTGKKFFREYPY
HHHHCCCCCEEEECCCCHHHCCEEEEECCCCHHHHHCCCCCCHHCCEEHHHHHHHHHCCC
REAFLLDKARKFRAELTMPLILLGGITNRETMDLAMAEGFEFVAMGRALLAEPDLINRIK
HHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
EDGGAHSVRSLCTHCNKCMATIYSHTHCVVTGAPDSLPVLG
HCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MDTQPDVFTPAKLGPVTLRNRIIKAATFEAATPNALVTDDLIHYHRLPAAGGVGMTTVAY
CCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
CAVAPGGRTDGWQIWMRPEAIPGLRRLTDAVHAEGAAISAQIGHAGPVANSRTNKAKALA
HHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHC
PVRFFNPLSMRFAKKATREDIAEVTAAHANAARLAIDSGFDAVEVHLGHNYLASSFLSPL
CHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCEEEEEECCHHHHHHHHHHH
INKRRDEFGGSLENRAKVARGVVRAVRDAVGNQIAVTAKLNMTDGVRAGIPLEESLQTAK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHH
WLEEDGGLDALELTAGSSLVNPLYLFHGDAPVKEFAGAFKPPLSWGIRMTGKKFFREYPY
HHHHCCCCCEEEECCCCHHHCCEEEEECCCCHHHHHCCCCCCHHCCEEHHHHHHHHHCCC
REAFLLDKARKFRAELTMPLILLGGITNRETMDLAMAEGFEFVAMGRALLAEPDLINRIK
HHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
EDGGAHSVRSLCTHCNKCMATIYSHTHCVVTGAPDSLPVLG
HCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2254270 [H]