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The map label for this gene is trmL [H]

Identifier: 119867253

GI number: 119867253

Start: 1289747

End: 1290286

Strand: Reverse

Name: trmL [H]

Synonym: Mkms_1203

Alternate gene names: 119867253

Gene position: 1290286-1289747 (Counterclockwise)

Preceding gene: 119867261

Following gene: 119867249

Centisome position: 22.49

GC content: 70.56

Gene sequence:

>540_bases
GTGTTCAAGGTGATGTTCTATTCACCCCGGATCGCGCCCAACACCGGCAACGCCATCCGCATGGTGGCGGGCACCGGATG
TGAGTTGCACCTGGTGCGCCCGTTGGGTTTCGACCTGTCGGAGCCGAAGCTGCGCCGGGCCGGGCTGGACTACCACGATC
TCGCCTCCGTCACCGTGCACGACGATCTCCCCGCCGCCTGGGATGCGGTGCTGCCCGCGTCGGTCTACGCGTTCACCGCG
CACGCGACCGTCTCGTTCGCCGACGTCGACTACCGGCCGGGCGATGTGCTGATGTTCGGGCCGGAGCCCACCGGACTGGA
CGCCGAGACGCTGGCCGATCCGCACATCACCGCGCAGGTGCGGATCCCGATGCTGGCCGGGCGGCGGTCACTGAACCTGT
CGAACGCCGCGGCGGTGGCCGTGTACGAGGCGTGGCGCCAGCACGGGTTCGCCGGCGGTGACATCAGACCCCGGGGCGAC
GTGAAGAGCGTGCCGCGCGCCGGGCGCCTTGGCCAACCGGGCGGACGCCGTGACCTGTAG

Upstream 100 bases:

>100_bases
GGCCACCCGGGGACCCCAAGTAGCTTGACTCCAGTAGGAAGTCAACCCCGGGCCGGCGACACGAGTCGACGTCGTCCGCC
ACGACAGACAGGTACCGGCT

Downstream 100 bases:

>100_bases
GAGCCGGCTGTAGTCCGGCGCATACGGAACCGGTGACGATCACACCGAAACGGCCACGCTCGAATGTGGCTCAACGCGGG
GTGTGGGTACTGCGTGTCTC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MFKVMFYSPRIAPNTGNAIRMVAGTGCELHLVRPLGFDLSEPKLRRAGLDYHDLASVTVHDDLPAAWDAVLPASVYAFTA
HATVSFADVDYRPGDVLMFGPEPTGLDAETLADPHITAQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGGDIRPRGD
VKSVPRAGRLGQPGGRRDL

Sequences:

>Translated_179_residues
MFKVMFYSPRIAPNTGNAIRMVAGTGCELHLVRPLGFDLSEPKLRRAGLDYHDLASVTVHDDLPAAWDAVLPASVYAFTA
HATVSFADVDYRPGDVLMFGPEPTGLDAETLADPHITAQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGGDIRPRGD
VKSVPRAGRLGQPGGRRDL
>Mature_179_residues
MFKVMFYSPRIAPNTGNAIRMVAGTGCELHLVRPLGFDLSEPKLRRAGLDYHDLASVTVHDDLPAAWDAVLPASVYAFTA
HATVSFADVDYRPGDVLMFGPEPTGLDAETLADPHITAQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGGDIRPRGD
VKSVPRAGRLGQPGGRRDL

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=156, Percent_Identity=47.4358974358974, Blast_Score=151, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 19317; Mature: 19317

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKVMFYSPRIAPNTGNAIRMVAGTGCELHLVRPLGFDLSEPKLRRAGLDYHDLASVTVH
CEEEEEECCEECCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHCCCCHHHCEEEEEE
DDLPAAWDAVLPASVYAFTAHATVSFADVDYRPGDVLMFGPEPTGLDAETLADPHITAQV
CCCCCHHHHHCCCEEEEEEECEEEEEEECCCCCCCEEEECCCCCCCCHHHCCCCCEEEEE
RIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGGDIRPRGDVKSVPRAGRLGQPGGRRDL
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFKVMFYSPRIAPNTGNAIRMVAGTGCELHLVRPLGFDLSEPKLRRAGLDYHDLASVTVH
CEEEEEECCEECCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHCCCCHHHCEEEEEE
DDLPAAWDAVLPASVYAFTAHATVSFADVDYRPGDVLMFGPEPTGLDAETLADPHITAQV
CCCCCHHHHHCCCEEEEEEECEEEEEEECCCCCCCEEEECCCCCCCCHHHCCCCCEEEEE
RIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGGDIRPRGDVKSVPRAGRLGQPGGRRDL
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA