Click here to switch to the map view.

The map label for this gene is rihA [H]

Identifier: 119867245

GI number: 119867245

Start: 1280269

End: 1281318

Strand: Reverse

Name: rihA [H]

Synonym: Mkms_1195

Alternate gene names: 119867245

Gene position: 1281318-1280269 (Counterclockwise)

Preceding gene: 119867246

Following gene: 119867239

Centisome position: 22.33

GC content: 70.1

Gene sequence:

>1050_bases
GTGACCATCCGCGACAACGAGTCTGCCGCTCCGATACCGGTTTTCGCCGATGTGGACACCGGCGTCGACGATGCGATGGC
GCTGGTGTACCTGTTCGCCAGCGAAGACGCCGACGTGGTCGGGATCGCCTCCACGGCGGGAAACGTCGGGGTGGACCAGG
TCTGCCACAACAATCTTGCGCTCCTCGACCTGTGCGGGACACACGACGTTCCGGTGTCGCGGGGCGCCGACGGCCCGATC
GCTTCGGCGTTGCGCACCGCCGAGGACACCCACGGTCCCGAGGGGCTGGGTTATGCGCACCTACCGTCGAGCGGTTCGAC
GGTCACCACCTACGACGCCGCCGAGGCGTGGGTGCGGGCCGCCCGCGCGCATCCGGGTGAGTTGGTCGGCATCGCGGTCG
GACCGCTGACCAACCTCGCGCTGGCGGTGCGGGCCGAACCGGCGCTGCCGACCCTGCTGCGCCGGTTGGTGATCATGGGC
GGCGCGTTCGACTACCGGGGCAACACGACCGCGGTGGCGGAATGGAACGTCAGCGTCGACCCCGAGGCCGCCGCCGAGGT
GTTCCACGCCTGGAGCGCCCCGGGTCTCACCCCGCCGATCGTGTTGGGGCTCAACCTGACCGAACACATCGCGTTGACGC
CGACACTGTTGACCAGGTTGGCGGCCGCCGCGCAGTCACCGACCAGGGCGATGAGCGTGCTCGACGACCGGGGCACCCGG
TCGGTGGCCGCCAACCCGCTGATCCGGGTGCTCGAGGATGCGTTGCGGTTCTACTTCGAGTTCCACTTCGATCAGGGTGA
GGGCTACCTCGCGCACCTGCACGACCCGCTGGCCGCCGCGGTCGCCCTCGACCCTGATCTGGTGCAGTACCGGGCCGCCA
CGGTGGATGTCGAGCTGACCGGGACGCTGACCCGTGGAATGACGATCGCGGACTGGAACGGGCGGTGGGGCCGCCCGCCG
AACGCCCTGGTCGGCATCGGCGTCGACGCCGGCGTCTTCTTCGACCGGTTCATCGACCGCGTCGGGCTGTTCGCCCGCCG
GCTGGGTTGA

Upstream 100 bases:

>100_bases
ATTCGGCCTGGCCGGCCTGACCCCCGACCAGCTCGCTGCGCTCAGTTGCGTCGAGACCCACGCCCGCACCCGGTGACCGG
AGCGGGCGTATCCTCGCCCG

Downstream 100 bases:

>100_bases
CATCAGTCGTCGGTGACGGTGACCGTGACGTCGATGTTGCCGCGCGTCGCGTTGGAGTACGGGCACACCTGATGCGCCTT
GTCGGCGATCTCCTGAGCGG

Product: inosine/uridine-preferring nucleoside hydrolase

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTIRDNESAAPIPVFADVDTGVDDAMALVYLFASEDADVVGIASTAGNVGVDQVCHNNLALLDLCGTHDVPVSRGADGPI
ASALRTAEDTHGPEGLGYAHLPSSGSTVTTYDAAEAWVRAARAHPGELVGIAVGPLTNLALAVRAEPALPTLLRRLVIMG
GAFDYRGNTTAVAEWNVSVDPEAAAEVFHAWSAPGLTPPIVLGLNLTEHIALTPTLLTRLAAAAQSPTRAMSVLDDRGTR
SVAANPLIRVLEDALRFYFEFHFDQGEGYLAHLHDPLAAAVALDPDLVQYRAATVDVELTGTLTRGMTIADWNGRWGRPP
NALVGIGVDAGVFFDRFIDRVGLFARRLG

Sequences:

>Translated_349_residues
MTIRDNESAAPIPVFADVDTGVDDAMALVYLFASEDADVVGIASTAGNVGVDQVCHNNLALLDLCGTHDVPVSRGADGPI
ASALRTAEDTHGPEGLGYAHLPSSGSTVTTYDAAEAWVRAARAHPGELVGIAVGPLTNLALAVRAEPALPTLLRRLVIMG
GAFDYRGNTTAVAEWNVSVDPEAAAEVFHAWSAPGLTPPIVLGLNLTEHIALTPTLLTRLAAAAQSPTRAMSVLDDRGTR
SVAANPLIRVLEDALRFYFEFHFDQGEGYLAHLHDPLAAAVALDPDLVQYRAATVDVELTGTLTRGMTIADWNGRWGRPP
NALVGIGVDAGVFFDRFIDRVGLFARRLG
>Mature_348_residues
TIRDNESAAPIPVFADVDTGVDDAMALVYLFASEDADVVGIASTAGNVGVDQVCHNNLALLDLCGTHDVPVSRGADGPIA
SALRTAEDTHGPEGLGYAHLPSSGSTVTTYDAAEAWVRAARAHPGELVGIAVGPLTNLALAVRAEPALPTLLRRLVIMGG
AFDYRGNTTAVAEWNVSVDPEAAAEVFHAWSAPGLTPPIVLGLNLTEHIALTPTLLTRLAAAAQSPTRAMSVLDDRGTRS
VAANPLIRVLEDALRFYFEFHFDQGEGYLAHLHDPLAAAVALDPDLVQYRAATVDVELTGTLTRGMTIADWNGRWGRPPN
ALVGIGVDAGVFFDRFIDRVGLFARRLG

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=335, Percent_Identity=33.4328358208955, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1788486, Length=324, Percent_Identity=29.0123456790123, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1786213, Length=322, Percent_Identity=31.3664596273292, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32565474, Length=320, Percent_Identity=24.375, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17565698, Length=316, Percent_Identity=27.5316455696203, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320608, Length=234, Percent_Identity=30.3418803418803, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24641839, Length=316, Percent_Identity=30.379746835443, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583915, Length=202, Percent_Identity=31.1881188118812, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24641837, Length=349, Percent_Identity=29.2263610315186, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.-

Molecular weight: Translated: 36844; Mature: 36713

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRDNESAAPIPVFADVDTGVDDAMALVYLFASEDADVVGIASTAGNVGVDQVCHNNLA
CCCCCCCCCCCCEEEECCCCCHHHHEEEEEEEECCCCCEEEEECCCCCCCHHHHHCCCEE
LLDLCGTHDVPVSRGADGPIASALRTAEDTHGPEGLGYAHLPSSGSTVTTYDAAEAWVRA
EEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEHHHHHHHHH
ARAHPGELVGIAVGPLTNLALAVRAEPALPTLLRRLVIMGGAFDYRGNTTAVAEWNVSVD
HHCCCCCEEEEEECCHHHEEEEEECCCCHHHHHHHHHHHCCCEECCCCEEEEEEECCCCC
PEAAAEVFHAWSAPGLTPPIVLGLNLTEHIALTPTLLTRLAAAAQSPTRAMSVLDDRGTR
HHHHHHHHHHHCCCCCCCCEEEECCCHHHEEECHHHHHHHHHHHCCHHHHHHHHHCCCCC
SVAANPLIRVLEDALRFYFEFHFDQGEGYLAHLHDPLAAAVALDPDLVQYRAATVDVELT
HHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCCCHHHHHCCCHHHHHEEEEEEEEEEE
GTLTRGMTIADWNGRWGRPPNALVGIGVDAGVFFDRFIDRVGLFARRLG
EEHCCCEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIRDNESAAPIPVFADVDTGVDDAMALVYLFASEDADVVGIASTAGNVGVDQVCHNNLA
CCCCCCCCCCCEEEECCCCCHHHHEEEEEEEECCCCCEEEEECCCCCCCHHHHHCCCEE
LLDLCGTHDVPVSRGADGPIASALRTAEDTHGPEGLGYAHLPSSGSTVTTYDAAEAWVRA
EEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEHHHHHHHHH
ARAHPGELVGIAVGPLTNLALAVRAEPALPTLLRRLVIMGGAFDYRGNTTAVAEWNVSVD
HHCCCCCEEEEEECCHHHEEEEEECCCCHHHHHHHHHHHCCCEECCCCEEEEEEECCCCC
PEAAAEVFHAWSAPGLTPPIVLGLNLTEHIALTPTLLTRLAAAAQSPTRAMSVLDDRGTR
HHHHHHHHHHHCCCCCCCCEEEECCCHHHEEECHHHHHHHHHHHCCHHHHHHHHHCCCCC
SVAANPLIRVLEDALRFYFEFHFDQGEGYLAHLHDPLAAAVALDPDLVQYRAATVDVELT
HHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCCCHHHHHCCCHHHHHEEEEEEEEEEE
GTLTRGMTIADWNGRWGRPPNALVGIGVDAGVFFDRFIDRVGLFARRLG
EEHCCCEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA