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The map label for this gene is artJ [H]

Identifier: 119866938

GI number: 119866938

Start: 969737

End: 970594

Strand: Reverse

Name: artJ [H]

Synonym: Mkms_0886

Alternate gene names: 119866938

Gene position: 970594-969737 (Counterclockwise)

Preceding gene: 119866939

Following gene: 119866937

Centisome position: 16.92

GC content: 65.97

Gene sequence:

>858_bases
ATGATGCGACGCCATCGGATGCCGATCGTAATGGGAGCCGCGGGAGCAGTGGGGGCCATCGCGCTCGCGCTGGTCTCCGC
GTGTTCGAGCAGCACCCCCGCCGAGAACTCCGGTGCCGAGGCCGATCCGGCCTCGCGCGAGATCGTCATCGGCACATCCG
GCGACTTCCCGCCGGTGTCGTTCCTCGAAGACGGCTCCACCGAGGTGGTCGGCTTCGAGAACGACCTCGCCAAGGGCGTG
ACCGACGACCTGGGCTGGACCTACCGATTCGAGCAGATCTCGTTCGACGGTCTGCTGCCCGCGCTGCAGTCCGGGCGCAT
CGACGCGATCCTGTCGGGCATGTACAACACGGAGAAACGCCGGGAGCAGGTCGATTTCATCGACTACATGCAGATCCCGC
TGTCTGTGCTGACGACGGTCGACAACGCCCCGAAGACCCCCGGGCCGGAGGCGCTGTGCGGGCAGTCGGTGGCCTATCTG
ACCGCGAGCCCGCCCGAACGTGCGCAGCTCGACGCGTGGTCGCAACAGTGCACCGACAGCGGTAAACCTCCGCTGCAGCC
GACCGGTTTCCCCTCCGTCGCACAAGGTGTCAACGCCGTCGCGACCGGTCGCGTGTACGCACAGCTCGAGGGTGACATCG
TGACCCTCTACATCTCGCGCACCGAATTCGGCGACAAACTCGACGTCGCGTTCAACGTCGAGGGCGAGAAGAGCGTCGTG
GGCATGGCGGTGGCGAAGGACAGCCCGCTCAAACCGGAACTGCAGAAGGCCATGGAGAGTTACGTCGCGTCCGAGGCGTA
CTGCCAGTCGGCCCAACAGTGGCAGTTCACGGCCGCCAATCCCATCCGCCAGTGTTGA

Upstream 100 bases:

>100_bases
GACGAATGGAACGGCTACCACAGCGTGGTCACGCAGTGGGAGGTCGACCGCTATCTCCTCGACGTCTGACACCGACACCC
ACGATCAGGAAGTGACCAGC

Downstream 100 bases:

>100_bases
CGGAGTTCTGGTATTACTTCACGCTGCCGGAACTGATCGACGGTGGCCTGCTGGCACTGCGGATCGCGCTGCTGTCGCTG
ATCATCGCCTGGCCGGCGGG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MMRRHRMPIVMGAAGAVGAIALALVSACSSSTPAENSGAEADPASREIVIGTSGDFPPVSFLEDGSTEVVGFENDLAKGV
TDDLGWTYRFEQISFDGLLPALQSGRIDAILSGMYNTEKRREQVDFIDYMQIPLSVLTTVDNAPKTPGPEALCGQSVAYL
TASPPERAQLDAWSQQCTDSGKPPLQPTGFPSVAQGVNAVATGRVYAQLEGDIVTLYISRTEFGDKLDVAFNVEGEKSVV
GMAVAKDSPLKPELQKAMESYVASEAYCQSAQQWQFTAANPIRQC

Sequences:

>Translated_285_residues
MMRRHRMPIVMGAAGAVGAIALALVSACSSSTPAENSGAEADPASREIVIGTSGDFPPVSFLEDGSTEVVGFENDLAKGV
TDDLGWTYRFEQISFDGLLPALQSGRIDAILSGMYNTEKRREQVDFIDYMQIPLSVLTTVDNAPKTPGPEALCGQSVAYL
TASPPERAQLDAWSQQCTDSGKPPLQPTGFPSVAQGVNAVATGRVYAQLEGDIVTLYISRTEFGDKLDVAFNVEGEKSVV
GMAVAKDSPLKPELQKAMESYVASEAYCQSAQQWQFTAANPIRQC
>Mature_285_residues
MMRRHRMPIVMGAAGAVGAIALALVSACSSSTPAENSGAEADPASREIVIGTSGDFPPVSFLEDGSTEVVGFENDLAKGV
TDDLGWTYRFEQISFDGLLPALQSGRIDAILSGMYNTEKRREQVDFIDYMQIPLSVLTTVDNAPKTPGPEALCGQSVAYL
TASPPERAQLDAWSQQCTDSGKPPLQPTGFPSVAQGVNAVATGRVYAQLEGDIVTLYISRTEFGDKLDVAFNVEGEKSVV
GMAVAKDSPLKPELQKAMESYVASEAYCQSAQQWQFTAANPIRQC

Specific function: Probably part of an ABC transporter complex involved in arginine transport. Binds arginine. Interacts with host epithelial cells, suggesting a role in host-cell adhesion during infection [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Secreted. Cell surface [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=129, Percent_Identity=31.0077519379845, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788649, Length=132, Percent_Identity=28.7878787878788, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30453; Mature: 30453

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRRHRMPIVMGAAGAVGAIALALVSACSSSTPAENSGAEADPASREIVIGTSGDFPPVS
CCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCH
FLEDGSTEVVGFENDLAKGVTDDLGWTYRFEQISFDGLLPALQSGRIDAILSGMYNTEKR
HCCCCCEEEEECHHHHHHCCHHHCCCEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHH
REQVDFIDYMQIPLSVLTTVDNAPKTPGPEALCGQSVAYLTASPPERAQLDAWSQQCTDS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHCC
GKPPLQPTGFPSVAQGVNAVATGRVYAQLEGDIVTLYISRTEFGDKLDVAFNVEGEKSVV
CCCCCCCCCCCHHHHCCCCEECCEEEEEECCCEEEEEEECCCCCCEEEEEEECCCCCCEE
GMAVAKDSPLKPELQKAMESYVASEAYCQSAQQWQFTAANPIRQC
EEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHCEEEECCCCCCCC
>Mature Secondary Structure
MMRRHRMPIVMGAAGAVGAIALALVSACSSSTPAENSGAEADPASREIVIGTSGDFPPVS
CCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCH
FLEDGSTEVVGFENDLAKGVTDDLGWTYRFEQISFDGLLPALQSGRIDAILSGMYNTEKR
HCCCCCEEEEECHHHHHHCCHHHCCCEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHH
REQVDFIDYMQIPLSVLTTVDNAPKTPGPEALCGQSVAYLTASPPERAQLDAWSQQCTDS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHCC
GKPPLQPTGFPSVAQGVNAVATGRVYAQLEGDIVTLYISRTEFGDKLDVAFNVEGEKSVV
CCCCCCCCCCCHHHHCCCCEECCEEEEEECCCEEEEEEECCCCCCEEEEEEECCCCCCEE
GMAVAKDSPLKPELQKAMESYVASEAYCQSAQQWQFTAANPIRQC
EEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9784136 [H]