Click here to switch to the map view.

The map label for this gene is yagI [H]

Identifier: 119866380

GI number: 119866380

Start: 359981

End: 360841

Strand: Reverse

Name: yagI [H]

Synonym: Mkms_0325

Alternate gene names: 119866380

Gene position: 360841-359981 (Counterclockwise)

Preceding gene: 119866381

Following gene: 119866379

Centisome position: 6.29

GC content: 72.59

Gene sequence:

>861_bases
ATGACGGTCCAACCAGGACTGGCTCCAGTCAGCCCGGAATCCACCCCCAGCGCGGTCATCGACCGGGTCTCGCTGGTGCT
CGATGCCTTCGACGGCCCCGGCCGGCTGACGCTGGCCCAGATCGTCCGGCGCACGGGGCTGCCCCGGTCGTCGGCGCACC
GCATGCTCGAACGCCTCGTGCAACTGCGCTGGCTGCGCCGCAACGGCCGCGACTACGAGCTGGGCATGCGGTTGGTCGAA
CTCGGGTCGCTGGCCGTACACCAGGACCGGCTGCACCGGGCGGCGACGCCGCTGCTGCACGATCTGCACCGCGCGACGGG
TCTGGTGGTGCACCTCGCCGTCCTGGACGGCGCCGACGTGGTCTACCTCGAGAAGATCGGCGACCGGATGGTGGCCGCAC
TCCCCAGCCGGGTCGGCGGCCGCCAGCCCGCGCACTGCACCGCGATCGGCAAGGCGCTGCTCGCCTACAACGCGGATGCG
TGCGACGACGTCGACCTCGACAATCGCCGGACCAAGTACTCCATCGGCAGCGCCGCCCAGCTGGCGAACGAACTCGCCAA
GACCCGCGCCCACGGTGTCGCCTTCGACCGCGAGGAGTCGCTACCCGGGTTCGGCTGTGTCGCAGCGCCGATCGGCGCAC
CCGGCGAAGCCGTCGCGGCGCTGTCCGTGTGCGGGCCGATGGGCCGCATGGTCTTCGACCAACGTCTCGCCGCGCCGGTC
CGGATGACCGCGATGGGCGTGTGGCGCAACGTCGAAGGCGGGCCGCAGCGCGTCGCGCCGACCCTGCAGCAGGTGCGGCC
ACTGCGCAGCGGCCCCACGCCGCGTCCGGCCCACGAGTCGACCGCCCTGCAGCTCGCATGA

Upstream 100 bases:

>100_bases
GTTTCAAGATCCAAGTGACACAGCCCACTCGGTTGTGATACAAACAGGCATATTCCTAATAGGAATATGCCTGATCTGCA
CTTCAGCCCCAGGAGGCGCC

Downstream 100 bases:

>100_bases
CCGGGGCGCTCGACCCGCAGATCGCCGACCTCATCGAGGGGCTGGACCGCGGCTTCCCACCGGTGCAGACCATGACCGGC
GCGCAGGCACGGGCCACGAT

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTVQPGLAPVSPESTPSAVIDRVSLVLDAFDGPGRLTLAQIVRRTGLPRSSAHRMLERLVQLRWLRRNGRDYELGMRLVE
LGSLAVHQDRLHRAATPLLHDLHRATGLVVHLAVLDGADVVYLEKIGDRMVAALPSRVGGRQPAHCTAIGKALLAYNADA
CDDVDLDNRRTKYSIGSAAQLANELAKTRAHGVAFDREESLPGFGCVAAPIGAPGEAVAALSVCGPMGRMVFDQRLAAPV
RMTAMGVWRNVEGGPQRVAPTLQQVRPLRSGPTPRPAHESTALQLA

Sequences:

>Translated_286_residues
MTVQPGLAPVSPESTPSAVIDRVSLVLDAFDGPGRLTLAQIVRRTGLPRSSAHRMLERLVQLRWLRRNGRDYELGMRLVE
LGSLAVHQDRLHRAATPLLHDLHRATGLVVHLAVLDGADVVYLEKIGDRMVAALPSRVGGRQPAHCTAIGKALLAYNADA
CDDVDLDNRRTKYSIGSAAQLANELAKTRAHGVAFDREESLPGFGCVAAPIGAPGEAVAALSVCGPMGRMVFDQRLAAPV
RMTAMGVWRNVEGGPQRVAPTLQQVRPLRSGPTPRPAHESTALQLA
>Mature_285_residues
TVQPGLAPVSPESTPSAVIDRVSLVLDAFDGPGRLTLAQIVRRTGLPRSSAHRMLERLVQLRWLRRNGRDYELGMRLVEL
GSLAVHQDRLHRAATPLLHDLHRATGLVVHLAVLDGADVVYLEKIGDRMVAALPSRVGGRQPAHCTAIGKALLAYNADAC
DDVDLDNRRTKYSIGSAAQLANELAKTRAHGVAFDREESLPGFGCVAAPIGAPGEAVAALSVCGPMGRMVFDQRLAAPVR
MTAMGVWRNVEGGPQRVAPTLQQVRPLRSGPTPRPAHESTALQLA

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=226, Percent_Identity=35.3982300884956, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1788131, Length=239, Percent_Identity=30.5439330543933, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788581, Length=219, Percent_Identity=30.5936073059361, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082359, Length=214, Percent_Identity=28.9719626168224, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1790752, Length=196, Percent_Identity=29.0816326530612, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789999, Length=230, Percent_Identity=25.2173913043478, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786715, Length=208, Percent_Identity=24.0384615384615, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 30688; Mature: 30557

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQPGLAPVSPESTPSAVIDRVSLVLDAFDGPGRLTLAQIVRRTGLPRSSAHRMLERLV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
QLRWLRRNGRDYELGMRLVELGSLAVHQDRLHRAATPLLHDLHRATGLVVHLAVLDGADV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
VYLEKIGDRMVAALPSRVGGRQPAHCTAIGKALLAYNADACDDVDLDNRRTKYSIGSAAQ
EHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHHH
LANELAKTRAHGVAFDREESLPGFGCVAAPIGAPGEAVAALSVCGPMGRMVFDQRLAAPV
HHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
RMTAMGVWRNVEGGPQRVAPTLQQVRPLRSGPTPRPAHESTALQLA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECC
>Mature Secondary Structure 
TVQPGLAPVSPESTPSAVIDRVSLVLDAFDGPGRLTLAQIVRRTGLPRSSAHRMLERLV
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
QLRWLRRNGRDYELGMRLVELGSLAVHQDRLHRAATPLLHDLHRATGLVVHLAVLDGADV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
VYLEKIGDRMVAALPSRVGGRQPAHCTAIGKALLAYNADACDDVDLDNRRTKYSIGSAAQ
EHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHHH
LANELAKTRAHGVAFDREESLPGFGCVAAPIGAPGEAVAALSVCGPMGRMVFDQRLAAPV
HHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
RMTAMGVWRNVEGGPQRVAPTLQQVRPLRSGPTPRPAHESTALQLA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]