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The map label for this gene is stp [H]

Identifier: 119855246

GI number: 119855246

Start: 67450

End: 68661

Strand: Reverse

Name: stp [H]

Synonym: Mkms_5860

Alternate gene names: 119855246

Gene position: 68661-67450 (Counterclockwise)

Preceding gene: 119855247

Following gene: 119855245

Centisome position: 31.68

GC content: 63.78

Gene sequence:

>1212_bases
GTGTCCAACCCAGCTGTTAATCTCCGGGCCACCTCCCTGCTGTGCAGCGTGGCTCCCAACGTCGAAACGCTGATCGGCGC
CCGCTTCCTGCAGGCGATCGGCGGTTCGATGCTCACTCCTGCTGGGTTGTCGATCGTCACGCAGGTGTTCACCGGCAGAG
TGGAGCGCGCGCGCGTCATCGGCATATGGGGGGGCGTCGTAGGCATCTCGATGGCCTTGGGTCCGATCATGGGCGGAATA
CTTCTCGAGTATGTTGACTGGCGAGCAGTGTTCTGGATCAACGTGCCGATCTGCGCGCTTGCGGTGCTGTTGACCGCGAT
CTTCGTTCCCGAATCCAAGTCGGTCACCATGCGCCACGTCGACCTGATCGGCTTGGTCCTGGGCATGGTCTTCCTGTTCG
GGCTGGTGTTCGTGCTCATCGAAGGGCCAGGGATGGGCTGGGCCGACACCCGCGTCGTCGTTAGCTGTTGCCTCGCAGCG
CTGGCCTTCGCGACATTCCTGCGCTACGAGTCACGCCGCGTCGACCCGTTCGTCGAGCTCCGATTCTTCCGCAGCATCCC
GTTTGCTTCGGCGACGGCCATCGCGGTGTGCGCATTCGCTGCATGGGGCGCGTTCCTGTTCATGATGTCAATCTATTTGC
AAGGGGAGCGCGGGCTTTCAGCGGTGAACACCGGGCTGATGTTTCTGCCCGTGGCCGTCGGCGCATTCATCTTCTCGCCC
TTGTCTGGCCGGCTGGTGGGGCGATTTGGTGCCAGACCGTCGCTGATGATCGCCGGCGCATTGATCGTCGCCGCGACTCT
CATGTTCGCGCAGATGAGCGACGCTACCCCGCAATGGCGAATGCTGGTCGCCTTTGCGGTTTTCGGCGTCGGTACTTCGA
TGGTGAACGCACCGGTCACAACCGCGGCCGTCAGCGGTATGCCGACCGACCGCGCCGGTGCGGCGGCGGCTATCACATCC
ACGAGCCGACAGGTGGGCGTCGCCATCGGTGTGGCCATATGTGGCCCGGTCGCTGGTGCGGCATTGGGCGACACCAACGT
CGATTTCGCGGTCTCCGCCCAGCCCCTATGGCTAGTGTTCGCTGGAGTTGGTGTGCTGATCTTCACGCTCGGTGTCTACT
CGACGTCGAAGCGCGCGCTGCGCTCAGCAGAGCATCTGGCGCCGCTGATCGCAGGAACCGATCCGCGACGGGAGGCTGCC
GATGTCGCGTAA

Upstream 100 bases:

>100_bases
CGGCGCAGCTACGCCAAAGGTGTCCGCGCCAAGCTCACCGACTACCGCGCCCAAGGACTGCTCTAGCCCCGCCACCTGCA
TCCAACGCGACCAATCGACG

Downstream 100 bases:

>100_bases
TCCGGAAGCGGACGAGGTCTGGCGCGCGATGACCGCACTGGTCACCGACAACCGCGACAGTTGGAGGCGAGCGACCGTCG
ACCAGACGGGCCTACCTTTC

Product: major facilitator transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSNPAVNLRATSLLCSVAPNVETLIGARFLQAIGGSMLTPAGLSIVTQVFTGRVERARVIGIWGGVVGISMALGPIMGGI
LLEYVDWRAVFWINVPICALAVLLTAIFVPESKSVTMRHVDLIGLVLGMVFLFGLVFVLIEGPGMGWADTRVVVSCCLAA
LAFATFLRYESRRVDPFVELRFFRSIPFASATAIAVCAFAAWGAFLFMMSIYLQGERGLSAVNTGLMFLPVAVGAFIFSP
LSGRLVGRFGARPSLMIAGALIVAATLMFAQMSDATPQWRMLVAFAVFGVGTSMVNAPVTTAAVSGMPTDRAGAAAAITS
TSRQVGVAIGVAICGPVAGAALGDTNVDFAVSAQPLWLVFAGVGVLIFTLGVYSTSKRALRSAEHLAPLIAGTDPRREAA
DVA

Sequences:

>Translated_403_residues
MSNPAVNLRATSLLCSVAPNVETLIGARFLQAIGGSMLTPAGLSIVTQVFTGRVERARVIGIWGGVVGISMALGPIMGGI
LLEYVDWRAVFWINVPICALAVLLTAIFVPESKSVTMRHVDLIGLVLGMVFLFGLVFVLIEGPGMGWADTRVVVSCCLAA
LAFATFLRYESRRVDPFVELRFFRSIPFASATAIAVCAFAAWGAFLFMMSIYLQGERGLSAVNTGLMFLPVAVGAFIFSP
LSGRLVGRFGARPSLMIAGALIVAATLMFAQMSDATPQWRMLVAFAVFGVGTSMVNAPVTTAAVSGMPTDRAGAAAAITS
TSRQVGVAIGVAICGPVAGAALGDTNVDFAVSAQPLWLVFAGVGVLIFTLGVYSTSKRALRSAEHLAPLIAGTDPRREAA
DVA
>Mature_402_residues
SNPAVNLRATSLLCSVAPNVETLIGARFLQAIGGSMLTPAGLSIVTQVFTGRVERARVIGIWGGVVGISMALGPIMGGIL
LEYVDWRAVFWINVPICALAVLLTAIFVPESKSVTMRHVDLIGLVLGMVFLFGLVFVLIEGPGMGWADTRVVVSCCLAAL
AFATFLRYESRRVDPFVELRFFRSIPFASATAIAVCAFAAWGAFLFMMSIYLQGERGLSAVNTGLMFLPVAVGAFIFSPL
SGRLVGRFGARPSLMIAGALIVAATLMFAQMSDATPQWRMLVAFAVFGVGTSMVNAPVTTAAVSGMPTDRAGAAAAITST
SRQVGVAIGVAICGPVAGAALGDTNVDFAVSAQPLWLVFAGVGVLIFTLGVYSTSKRALRSAEHLAPLIAGTDPRREAAD
VA

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=328, Percent_Identity=27.4390243902439, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790195, Length=336, Percent_Identity=25, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788710, Length=327, Percent_Identity=24.1590214067278, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788392, Length=335, Percent_Identity=27.1641791044776, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789042, Length=367, Percent_Identity=23.433242506812, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324954, Length=393, Percent_Identity=21.3740458015267, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42144; Mature: 42013

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPAVNLRATSLLCSVAPNVETLIGARFLQAIGGSMLTPAGLSIVTQVFTGRVERARVI
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEE
GIWGGVVGISMALGPIMGGILLEYVDWRAVFWINVPICALAVLLTAIFVPESKSVTMRHV
EEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHH
DLIGLVLGMVFLFGLVFVLIEGPGMGWADTRVVVSCCLAALAFATFLRYESRRVDPFVEL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RFFRSIPFASATAIAVCAFAAWGAFLFMMSIYLQGERGLSAVNTGLMFLPVAVGAFIFSP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHCC
LSGRLVGRFGARPSLMIAGALIVAATLMFAQMSDATPQWRMLVAFAVFGVGTSMVNAPVT
CCCCHHECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHCCCHH
TAAVSGMPTDRAGAAAAITSTSRQVGVAIGVAICGPVAGAALGDTNVDFAVSAQPLWLVF
HHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHH
AGVGVLIFTLGVYSTSKRALRSAEHLAPLIAGTDPRREAADVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure 
SNPAVNLRATSLLCSVAPNVETLIGARFLQAIGGSMLTPAGLSIVTQVFTGRVERARVI
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEE
GIWGGVVGISMALGPIMGGILLEYVDWRAVFWINVPICALAVLLTAIFVPESKSVTMRHV
EEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHH
DLIGLVLGMVFLFGLVFVLIEGPGMGWADTRVVVSCCLAALAFATFLRYESRRVDPFVEL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RFFRSIPFASATAIAVCAFAAWGAFLFMMSIYLQGERGLSAVNTGLMFLPVAVGAFIFSP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHCC
LSGRLVGRFGARPSLMIAGALIVAATLMFAQMSDATPQWRMLVAFAVFGVGTSMVNAPVT
CCCCHHECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHCCCHH
TAAVSGMPTDRAGAAAAITSTSRQVGVAIGVAICGPVAGAALGDTNVDFAVSAQPLWLVF
HHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHH
AGVGVLIFTLGVYSTSKRALRSAEHLAPLIAGTDPRREAADVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]