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The map label for this gene is insF1 [C]

Identifier: 119855214

GI number: 119855214

Start: 37888

End: 38718

Strand: Reverse

Name: insF1 [C]

Synonym: Mkms_5828

Alternate gene names: 119855214

Gene position: 38718-37888 (Counterclockwise)

Preceding gene: 119855215

Following gene: 119855213

Centisome position: 17.86

GC content: 63.9

Gene sequence:

>831_bases
GTGGAGTTCATCAGCGCTCACCAGCACACTCGGGTGGGCGCTGATGGTCTCAAGTGGGGTGTCGAGTCGATGTGCGCCGT
GCTCTCGGAGTTCGGCGTCGCGATCGCCCCGTCGACGTATTACGCCCACCGCGCCCGCGGTGGCCCCTCGAGGGCCGATT
GGTCTGACGCGCAGGTGATCGATGCGATCTACCGGCTCCGTCAATCCAACCCGCTGTATCGGGTCCTGGGTGCTCGCAAG
ACATGGATTGTGTTGCGTACCAACGGACTCAATGTTTCGCGGTGCGTGGTGGAGCGGGTCATGCGGGAGATGGGCTGGCG
CGGTGCGTGCAAGCGGCGCCGGGTCCGCACCACCGTCGCCGACCCGGCGGCGACACGGGCTCCAGATCATGTTCGGCGGC
GGTTCGTCGCCGAGGCGCCTGACCGATTGTGGGTGGCTGATTTCACGTATTGCCGGACCCGGGCCGGCTGGGCTTATGCG
GCGTTCGTCACCGATGTCTATGCCCGCAAGATCGTCGGCTGGAAGGTGGCTACCGAGATGACGCAGAAGCTGGTGACTGA
TGCGATCAGTCACGCGATTGATACCAGAAAGCGTTCTGGTACAACATCTTTAGATTCACTTATCCACCACAGCGATGCGG
GCTCTCAAGGCGGATTCAACTGGTCGTCGCAACACCTTGATCACGGAGGTGTGGTGTGGCCAAGCGTAAGGACGCGGAAT
CGGTTGGTAGGCGACGGCAGTGGGCGGCTGACCGTGCGTTGCGGCCTGCGATGCGCTCACCAGGGCGCCCGGACCCGTCG
CGGTCGGTGCAGCGTCAGTTTTGGCGGCTGA

Upstream 100 bases:

>100_bases
AGCTGCGCCGGGAGAATGCCGAACTCAAGCGGGCCAACGCGATCTTGAAGGCGGCGTCGGTTTTCTTCGCTGCCGAGCTC
GACCGGCCCTGTCAGTAGTC

Downstream 100 bases:

>100_bases
TCGCCCAGGGTGTCTCCACCGACGACGCAGCCGCAGAGGTCGGCGTGTCGACACCGGTGGCGACCAGGTGGTTCCACCAC
GCTGGCGGCATGACGCCGAT

Product: putative transposase for insertion element IS6110 (second part)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MEFISAHQHTRVGADGLKWGVESMCAVLSEFGVAIAPSTYYAHRARGGPSRADWSDAQVIDAIYRLRQSNPLYRVLGARK
TWIVLRTNGLNVSRCVVERVMREMGWRGACKRRRVRTTVADPAATRAPDHVRRRFVAEAPDRLWVADFTYCRTRAGWAYA
AFVTDVYARKIVGWKVATEMTQKLVTDAISHAIDTRKRSGTTSLDSLIHHSDAGSQGGFNWSSQHLDHGGVVWPSVRTRN
RLVGDGSGRLTVRCGLRCAHQGARTRRGRCSVSFGG

Sequences:

>Translated_276_residues
MEFISAHQHTRVGADGLKWGVESMCAVLSEFGVAIAPSTYYAHRARGGPSRADWSDAQVIDAIYRLRQSNPLYRVLGARK
TWIVLRTNGLNVSRCVVERVMREMGWRGACKRRRVRTTVADPAATRAPDHVRRRFVAEAPDRLWVADFTYCRTRAGWAYA
AFVTDVYARKIVGWKVATEMTQKLVTDAISHAIDTRKRSGTTSLDSLIHHSDAGSQGGFNWSSQHLDHGGVVWPSVRTRN
RLVGDGSGRLTVRCGLRCAHQGARTRRGRCSVSFGG
>Mature_276_residues
MEFISAHQHTRVGADGLKWGVESMCAVLSEFGVAIAPSTYYAHRARGGPSRADWSDAQVIDAIYRLRQSNPLYRVLGARK
TWIVLRTNGLNVSRCVVERVMREMGWRGACKRRRVRTTVADPAATRAPDHVRRRFVAEAPDRLWVADFTYCRTRAGWAYA
AFVTDVYARKIVGWKVATEMTQKLVTDAISHAIDTRKRSGTTSLDSLIHHSDAGSQGGFNWSSQHLDHGGVVWPSVRTRN
RLVGDGSGRLTVRCGLRCAHQGARTRRGRCSVSFGG

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=209, Percent_Identity=38.755980861244, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1788405, Length=219, Percent_Identity=27.3972602739726, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787263, Length=219, Percent_Identity=27.3972602739726, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786753, Length=219, Percent_Identity=27.3972602739726, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786570, Length=219, Percent_Identity=27.3972602739726, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786490, Length=219, Percent_Identity=27.3972602739726, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 30740; Mature: 30740

Theoretical pI: Translated: 11.29; Mature: 11.29

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFISAHQHTRVGADGLKWGVESMCAVLSEFGVAIAPSTYYAHRARGGPSRADWSDAQVI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHCCCCCCCCCCCHHHHH
DAIYRLRQSNPLYRVLGARKTWIVLRTNGLNVSRCVVERVMREMGWRGACKRRRVRTTVA
HHHHHHHCCCCHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
DPAATRAPDHVRRRFVAEAPDRLWVADFTYCRTRAGWAYAAFVTDVYARKIVGWKVATEM
CCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TQKLVTDAISHAIDTRKRSGTTSLDSLIHHSDAGSQGGFNWSSQHLDHGGVVWPSVRTRN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCCEECCCCCCCC
RLVGDGSGRLTVRCGLRCAHQGARTRRGRCSVSFGG
CEEECCCCEEEEEECCHHHHCCCCCCCCEEEEECCC
>Mature Secondary Structure
MEFISAHQHTRVGADGLKWGVESMCAVLSEFGVAIAPSTYYAHRARGGPSRADWSDAQVI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHCCCCCCCCCCCHHHHH
DAIYRLRQSNPLYRVLGARKTWIVLRTNGLNVSRCVVERVMREMGWRGACKRRRVRTTVA
HHHHHHHCCCCHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
DPAATRAPDHVRRRFVAEAPDRLWVADFTYCRTRAGWAYAAFVTDVYARKIVGWKVATEM
CCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TQKLVTDAISHAIDTRKRSGTTSLDSLIHHSDAGSQGGFNWSSQHLDHGGVVWPSVRTRN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCCEECCCCCCCC
RLVGDGSGRLTVRCGLRCAHQGARTRRGRCSVSFGG
CEEECCCCEEEEEECCHHHHCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]