| Definition | Mycobacterium sp. KMS plasmid pMKMS01, complete sequence. |
|---|---|
| Accession | NC_008703 |
| Length | 302,089 |
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The map label for this gene is aas [H]
Identifier: 119855133
GI number: 119855133
Start: 254725
End: 257220
Strand: Reverse
Name: aas [H]
Synonym: Mkms_5747
Alternate gene names: 119855133
Gene position: 257220-254725 (Counterclockwise)
Preceding gene: 119855134
Following gene: 119855126
Centisome position: 85.15
GC content: 70.43
Gene sequence:
>2496_bases ATGATGTTCCAACGGCTCGTCGACCACCTCGCCTGCCACGGTGGCGACGTCGCGGTGATCACCGCCGAGAAGAGGATCAG CTACGCCGAACTCGCGACTCGGGTGGCCGCCCGGGCCGCGGATCTGGGCGCGCGCCGCAAGCTGGTCCTGCTCGAAACCC GCAACGACCTACCCACGCTGGTGACCTACCTCGGCGCGCTCGCCGGCCACCACGTCGTGCTGCCCGTCCCGGCGGGCGGC GACCACCGATCGGTCGTCGAGACCTACCACCCGGACGTCATCGTCGCCGATGGTGTCATCACCGAGGGACCACGCCACCG TCCCGCCCCCGAACTCCACCCCGACCTGGCCCTGCTGCTCTCGACGTCCGGCAGCACCGGCTCCCCCAAGCTGGTCCGGT TGTCCCGGTTCAACCTCGCCGCCAACGCCGAGTCCATCGCCACCTACCTCGACATCACCCGAACCGACAGGGCCGCAACG ACTCTGCCGATGTCGTACTGCTACGGGCTGTCGGTGATCCACAGTCACCTGCTGCGCGGAGCCGCACTCGTGCTCACCGA ACTGTCCGTCGCCAACGACGACTTCTGGGAGTCGTTCCGCAGCCACGGCGCAACGTCGTTCGCGGGCGTACCGCACACCT TCGAACTGCTCGACCGGATCGGCTTCGAGGCGATGTCGCTGCCGAACCTGCGCTACGTCACCCAGGCCGGCGGACGGCTC GACCCGGATCGCGTGCGGCGGCTCGCCGGTCTCGGCGGGTGGGCGGGGTGGCGGTTCTTCGTCATGTACGGCGCCACCGA GGCCACCGCGCGCATGGCGTACCTGCCGCCGGACCTCGCCCACGACCATCCCGAGTCGATCGGCGTTCCGATCCCCGGCG GGTCGTTTCGCATCGAGCCGGTCGACGGGATGCCCGACGGCACGGGTGAACTCGTCTACCGTGGACCGAACGTGATGATG GGCTACGCCCACACCGCCGCCGACCTGGCCGTCGGTGCCGTGATCGAGGAACTGCGCACCGGCGACATCGCCCACCGGCG CCCTGATGGCCTCTACGAGGTGGTCGGCCGCAGCGACCGGTTCGTGAAGCTGTACGGGCTGCGCATCGACCTGCAACGCG TCGAGTCGGCCCTGCACAGCGACAGCCTCGACGCGATCTGCACCGCGGGCGACGGCCTCCTGATCGTCGGCGCGGCCGAC CGTCCAAGGGACTGCGACGTCGCCGCCGCGGCGGCCGACGCGTCCGGGCTTCCCCGGGCGGCGATCCGGGTGATCGACGT CGACGAGATCCCACGGCTGCCCACCGGCAAGCCGGACTACCGCAGCCTGCGGGAGCGCGCCGCCGAGCGGACGACCGACG ACGCTCGTCTGGACCTGCGTGGCCTGTTCGCCGACGTGCTGCAGATCGACGCGGCCGACGTCGATCCCCGCAGCAGCTTC GTCGACCTCGGCGGCAACTCGCTGTCCTACGTCGCCATGTCGGTGCGGCTCGAACGCGTACTCGGGCACCTGCCTGCGGA CTGGCAGCGGCTGCCGCTGAGCGAGCTGGAGTCCGCCCGCAGGACCCGCCGGTGGTGGGGAACGACGCTGGAGACCAGCG TGGCGCTGCGTGCGGCGGCGATCGTCCTGGTCGTCGGCTCGCACGCCGAGCTGTGGGTGCTGTGGGGCGGCGCGCACATC CTGCTGGGCGTCGCCGGATACAACTTCGGCCGCTTCTGCCTCACGCCCCTGCCGCGCGCCACCCGGGTGCGCCACCTGGT CAGCACCATCGGCTGGATCGCCGGGCCCGCCATCCTGTGGGTGGCGTTCGCGCTACTCGTCACCGACGACTACGCGGCCT CGAACTTATTGCTGGCCAACAAGCTCCTCGGCCCTGAGGACAGCATGACCGCCGGGCGGTTGTGGTTCGTCGAAGTCCTG GTGTGGGTCCTGGTGGGGTTGGCGCTGCTATGCGCGACGCGGTGGGGCGATCGCGCCGAACGCCGGTGGCCGTTCGGCGT AGCCGCGGCATTCCTGGCCCTGGGCATGGCCCTGCGGTGGGACGTCTTCGGCACCGGACTGGGCAAGGCTGCGTGGTTCA CCATGCTGGCGTTCTGGTTCTTCGCCATCGGCTGGGCCGCCTCGAAGTCGTCGACGGTGTGGCAGCGGCTCGCCGTGACC ACGGCCCTCGCCGTCGGCATCGTCGGGTACTTCGGCCAACCCCACCGCGAGGCCCTGGTGTTCATCGGGCTGGCGCTGCT GATCTGGCTGCCCGCCATCCGCTGCCCGTCGGCGTTCACGACCACCGCAGGCGTGATCGCCGAGGCGTCACTGTTCATCT ACCTGACGCACTACCAGGTGTATCCGCTGTTCGGCGACCAGAGGCTCGTCGGCGTGCTGGCCTCGGTCATCGTCGGTATC CTGCTGACCCAGCTGGTCACCGTGGCAAGGAGTCGACTCCGCCGGCGCACGCCGACCGGACCCACACGACGGCAGGCTGC TCCCGCTCGGCGATGA
Upstream 100 bases:
>100_bases GCGTCGCGCCGCCCGCGGTCGACGCGAAGGTCCGCATCCACGTCACCGGCGCCGCCGTCGTGTTCGGTGACCGCCCCACG CGAGCGCCGATGACGGCGCG
Downstream 100 bases:
>100_bases GCCCCTCCTGCGCCCGCGTGGCCGCGATGACCCCTACGAACCCGGAATCTCTAGCCGCGCGTCCGCCATCGACACGAATT CTGACCACAGGCGCCGCGGG
Product: AMP-dependent synthetase and ligase
Products: NA
Alternate protein names: 2-acylglycerophosphoethanolamine acyltransferase; 2-acyl-GPE acyltransferase; Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferase; Acyl-[acyl-carrier-protein] synthetase; Acyl-ACP synthetase; Long-chain-fatty-acid--[acyl-carrier-protein] ligase [H]
Number of amino acids: Translated: 831; Mature: 831
Protein sequence:
>831_residues MMFQRLVDHLACHGGDVAVITAEKRISYAELATRVAARAADLGARRKLVLLETRNDLPTLVTYLGALAGHHVVLPVPAGG DHRSVVETYHPDVIVADGVITEGPRHRPAPELHPDLALLLSTSGSTGSPKLVRLSRFNLAANAESIATYLDITRTDRAAT TLPMSYCYGLSVIHSHLLRGAALVLTELSVANDDFWESFRSHGATSFAGVPHTFELLDRIGFEAMSLPNLRYVTQAGGRL DPDRVRRLAGLGGWAGWRFFVMYGATEATARMAYLPPDLAHDHPESIGVPIPGGSFRIEPVDGMPDGTGELVYRGPNVMM GYAHTAADLAVGAVIEELRTGDIAHRRPDGLYEVVGRSDRFVKLYGLRIDLQRVESALHSDSLDAICTAGDGLLIVGAAD RPRDCDVAAAAADASGLPRAAIRVIDVDEIPRLPTGKPDYRSLRERAAERTTDDARLDLRGLFADVLQIDAADVDPRSSF VDLGGNSLSYVAMSVRLERVLGHLPADWQRLPLSELESARRTRRWWGTTLETSVALRAAAIVLVVGSHAELWVLWGGAHI LLGVAGYNFGRFCLTPLPRATRVRHLVSTIGWIAGPAILWVAFALLVTDDYAASNLLLANKLLGPEDSMTAGRLWFVEVL VWVLVGLALLCATRWGDRAERRWPFGVAAAFLALGMALRWDVFGTGLGKAAWFTMLAFWFFAIGWAASKSSTVWQRLAVT TALAVGIVGYFGQPHREALVFIGLALLIWLPAIRCPSAFTTTAGVIAEASLFIYLTHYQVYPLFGDQRLVGVLASVIVGI LLTQLVTVARSRLRRRTPTGPTRRQAAPARR
Sequences:
>Translated_831_residues MMFQRLVDHLACHGGDVAVITAEKRISYAELATRVAARAADLGARRKLVLLETRNDLPTLVTYLGALAGHHVVLPVPAGG DHRSVVETYHPDVIVADGVITEGPRHRPAPELHPDLALLLSTSGSTGSPKLVRLSRFNLAANAESIATYLDITRTDRAAT TLPMSYCYGLSVIHSHLLRGAALVLTELSVANDDFWESFRSHGATSFAGVPHTFELLDRIGFEAMSLPNLRYVTQAGGRL DPDRVRRLAGLGGWAGWRFFVMYGATEATARMAYLPPDLAHDHPESIGVPIPGGSFRIEPVDGMPDGTGELVYRGPNVMM GYAHTAADLAVGAVIEELRTGDIAHRRPDGLYEVVGRSDRFVKLYGLRIDLQRVESALHSDSLDAICTAGDGLLIVGAAD RPRDCDVAAAAADASGLPRAAIRVIDVDEIPRLPTGKPDYRSLRERAAERTTDDARLDLRGLFADVLQIDAADVDPRSSF VDLGGNSLSYVAMSVRLERVLGHLPADWQRLPLSELESARRTRRWWGTTLETSVALRAAAIVLVVGSHAELWVLWGGAHI LLGVAGYNFGRFCLTPLPRATRVRHLVSTIGWIAGPAILWVAFALLVTDDYAASNLLLANKLLGPEDSMTAGRLWFVEVL VWVLVGLALLCATRWGDRAERRWPFGVAAAFLALGMALRWDVFGTGLGKAAWFTMLAFWFFAIGWAASKSSTVWQRLAVT TALAVGIVGYFGQPHREALVFIGLALLIWLPAIRCPSAFTTTAGVIAEASLFIYLTHYQVYPLFGDQRLVGVLASVIVGI LLTQLVTVARSRLRRRTPTGPTRRQAAPARR >Mature_831_residues MMFQRLVDHLACHGGDVAVITAEKRISYAELATRVAARAADLGARRKLVLLETRNDLPTLVTYLGALAGHHVVLPVPAGG DHRSVVETYHPDVIVADGVITEGPRHRPAPELHPDLALLLSTSGSTGSPKLVRLSRFNLAANAESIATYLDITRTDRAAT TLPMSYCYGLSVIHSHLLRGAALVLTELSVANDDFWESFRSHGATSFAGVPHTFELLDRIGFEAMSLPNLRYVTQAGGRL DPDRVRRLAGLGGWAGWRFFVMYGATEATARMAYLPPDLAHDHPESIGVPIPGGSFRIEPVDGMPDGTGELVYRGPNVMM GYAHTAADLAVGAVIEELRTGDIAHRRPDGLYEVVGRSDRFVKLYGLRIDLQRVESALHSDSLDAICTAGDGLLIVGAAD RPRDCDVAAAAADASGLPRAAIRVIDVDEIPRLPTGKPDYRSLRERAAERTTDDARLDLRGLFADVLQIDAADVDPRSSF VDLGGNSLSYVAMSVRLERVLGHLPADWQRLPLSELESARRTRRWWGTTLETSVALRAAAIVLVVGSHAELWVLWGGAHI LLGVAGYNFGRFCLTPLPRATRVRHLVSTIGWIAGPAILWVAFALLVTDDYAASNLLLANKLLGPEDSMTAGRLWFVEVL VWVLVGLALLCATRWGDRAERRWPFGVAAAFLALGMALRWDVFGTGLGKAAWFTMLAFWFFAIGWAASKSSTVWQRLAVT TALAVGIVGYFGQPHREALVFIGLALLIWLPAIRCPSAFTTTAGVIAEASLFIYLTHYQVYPLFGDQRLVGVLASVIVGI LLTQLVTVARSRLRRRTPTGPTRRQAAPARR
Specific function: Plays a role in lysophospholipid acylation. Transfers fatty acids to the 1-position via an enzyme-bound acyl-ACP intermediate in the presence of ATP and magnesium. Its physiological function is to regenerate phosphatidylethanolamine from 2-acyl-glycero-3-
COG id: COG0318
COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II
Gene ontology:
Cell location: Cell inner membrane; Multi-pass membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the ATP- dependent AMP-binding enzyme family [H]
Homologues:
Organism=Homo sapiens, GI45580730, Length=426, Percent_Identity=24.1784037558685, Blast_Score=67, Evalue=7e-11, Organism=Escherichia coli, GI1789201, Length=397, Percent_Identity=27.2040302267003, Blast_Score=101, Evalue=2e-22, Organism=Escherichia coli, GI1788107, Length=319, Percent_Identity=27.5862068965517, Blast_Score=74, Evalue=5e-14, Organism=Escherichia coli, GI145693145, Length=367, Percent_Identity=23.7057220708447, Blast_Score=65, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17556356, Length=347, Percent_Identity=27.9538904899135, Blast_Score=72, Evalue=1e-12, Organism=Saccharomyces cerevisiae, GI6319699, Length=366, Percent_Identity=24.0437158469945, Blast_Score=80, Evalue=9e-16, Organism=Drosophila melanogaster, GI18859661, Length=358, Percent_Identity=26.8156424581006, Blast_Score=96, Evalue=1e-19,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002123 - InterPro: IPR020845 - InterPro: IPR000873 [H]
Pfam domain/function: PF01553 Acyltransferase; PF00501 AMP-binding [H]
EC number: =2.3.1.40; =6.2.1.20 [H]
Molecular weight: Translated: 90335; Mature: 90335
Theoretical pI: Translated: 8.29; Mature: 8.29
Prosite motif: PS50075 ACP_DOMAIN
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 1.6 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 1.6 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMFQRLVDHLACHGGDVAVITAEKRISYAELATRVAARAADLGARRKLVLLETRNDLPTL CCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHH VTYLGALAGHHVVLPVPAGGDHRSVVETYHPDVIVADGVITEGPRHRPAPELHPDLALLL HHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCEEEECCEECCCCCCCCCCCCCCCEEEEE STSGSTGSPKLVRLSRFNLAANAESIATYLDITRTDRAATTLPMSYCYGLSVIHSHLLRG ECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHH AALVLTELSVANDDFWESFRSHGATSFAGVPHTFELLDRIGFEAMSLPNLRYVTQAGGRL HHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHCCCCCEEHHCCCCCC DPDRVRRLAGLGGWAGWRFFVMYGATEATARMAYLPPDLAHDHPESIGVPIPGGSFRIEP CHHHHHHHHCCCCCHHEEEEEEECCCHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEEE VDGMPDGTGELVYRGPNVMMGYAHTAADLAVGAVIEELRTGDIAHRRPDGLYEVVGRSDR CCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCC FVKLYGLRIDLQRVESALHSDSLDAICTAGDGLLIVGAADRPRDCDVAAAAADASGLPRA EEEEEEEEEEHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHCCCCCCCCHH AIRVIDVDEIPRLPTGKPDYRSLRERAAERTTDDARLDLRGLFADVLQIDAADVDPRSSF HEEEEEHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCE VDLGGNSLSYVAMSVRLERVLGHLPADWQRLPLSELESARRTRRWWGTTLETSVALRAAA EECCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHEE IVLVVGSHAELWVLWGGAHILLGVAGYNFGRFCLTPLPRATRVRHLVSTIGWIAGPAILW EEEEECCCCEEEEEECCCEEEEEECCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHH VAFALLVTDDYAASNLLLANKLLGPEDSMTAGRLWFVEVLVWVLVGLALLCATRWGDRAE HHHHHHHCCCCHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC RRWPFGVAAAFLALGMALRWDVFGTGLGKAAWFTMLAFWFFAIGWAASKSSTVWQRLAVT CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH TALAVGIVGYFGQPHREALVFIGLALLIWLPAIRCPSAFTTTAGVIAEASLFIYLTHYQV HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEEEE YPLFGDQRLVGVLASVIVGILLTQLVTVARSRLRRRTPTGPTRRQAAPARR EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC >Mature Secondary Structure MMFQRLVDHLACHGGDVAVITAEKRISYAELATRVAARAADLGARRKLVLLETRNDLPTL CCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHH VTYLGALAGHHVVLPVPAGGDHRSVVETYHPDVIVADGVITEGPRHRPAPELHPDLALLL HHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCEEEECCEECCCCCCCCCCCCCCCEEEEE STSGSTGSPKLVRLSRFNLAANAESIATYLDITRTDRAATTLPMSYCYGLSVIHSHLLRG ECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHH AALVLTELSVANDDFWESFRSHGATSFAGVPHTFELLDRIGFEAMSLPNLRYVTQAGGRL HHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHCCCCCEEHHCCCCCC DPDRVRRLAGLGGWAGWRFFVMYGATEATARMAYLPPDLAHDHPESIGVPIPGGSFRIEP CHHHHHHHHCCCCCHHEEEEEEECCCHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEEE VDGMPDGTGELVYRGPNVMMGYAHTAADLAVGAVIEELRTGDIAHRRPDGLYEVVGRSDR CCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCC FVKLYGLRIDLQRVESALHSDSLDAICTAGDGLLIVGAADRPRDCDVAAAAADASGLPRA EEEEEEEEEEHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHCCCCCCCCHH AIRVIDVDEIPRLPTGKPDYRSLRERAAERTTDDARLDLRGLFADVLQIDAADVDPRSSF HEEEEEHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCE VDLGGNSLSYVAMSVRLERVLGHLPADWQRLPLSELESARRTRRWWGTTLETSVALRAAA EECCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHEE IVLVVGSHAELWVLWGGAHILLGVAGYNFGRFCLTPLPRATRVRHLVSTIGWIAGPAILW EEEEECCCCEEEEEECCCEEEEEECCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHH VAFALLVTDDYAASNLLLANKLLGPEDSMTAGRLWFVEVLVWVLVGLALLCATRWGDRAE HHHHHHHCCCCHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC RRWPFGVAAAFLALGMALRWDVFGTGLGKAAWFTMLAFWFFAIGWAASKSSTVWQRLAVT CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH TALAVGIVGYFGQPHREALVFIGLALLIWLPAIRCPSAFTTTAGVIAEASLFIYLTHYQV HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEEEE YPLFGDQRLVGVLASVIVGILLTQLVTVARSRLRRRTPTGPTRRQAAPARR EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: NA