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The map label for this gene is rhtB [H]

Identifier: 119776763

GI number: 119776763

Start: 4274700

End: 4275323

Strand: Direct

Name: rhtB [H]

Synonym: Sama_3631

Alternate gene names: 119776763

Gene position: 4274700-4275323 (Clockwise)

Preceding gene: 119776762

Following gene: 119776766

Centisome position: 99.27

GC content: 54.17

Gene sequence:

>624_bases
ATGAGTCTGGATACCTTTCTACTCTACCTTGCCGCCATTCTCTTGATTGCCATTTCCCCGGGCCCCATGGCTATGCTGTC
CATGTCCCACGGCATGCACTTTGGTAAAAGGCGCTCACTCGCCACAGCTCTGGGCAGCGTGCTGGCAGCTCTGTGTTTAA
TGCTGGCGTCAGCAGCTGGCCTCGGTGCGCTGCTGGCCGCGACCGAATACGGTTTTGTGCTGCTAAAATGGTGTGGTGCC
GCTTACCTTTTGTATCTGGGGATGAGACTGCTGCTGACCAAGCCACAACCAAGCGATTTCAAGCTTGAGTCGCCAAAAGG
AAAGGGACGGAGAAGGGATCTGTTTCAGCAGGCGTTTCTGGTGGGAATAAGCAATCCCAAAGACTTGCTGTTTTTCGCAG
CACTGTTTCCGCAGTTTATCGATGTGAGTGCCCCTCAGGGGGCACAGCTGGCGATACTGGCCAGTAGTTGGGCGATAGTG
GACTTCAGTTTTGTGATGATTTACGCCAGCCTGGCCAGTTTGCTGGCACCCAAACTCAGTGCCAGCAATCGTCTGCATTG
GTTCGACAGAACCAGTGGCGGCGTCTTTGTCAGCCTCGCTGCCCTTCTGGCTATCAAGAATTAA

Upstream 100 bases:

>100_bases
ATTATGTACCCTAGGAAAAAGACAAAAGCGGCAAATAGCCTTTGCCTGCGGTGGCCGAACTGGCTATAGTTCGGCCAATC
GTATTTTTGTATACAATTCC

Downstream 100 bases:

>100_bases
CCGGCCGTAAACTGCGAATATCTTCCCCGGTGGGATTTTGGAATGCCGTCAAACCAAATTCCGGCAGAATCGCAAAGATG
TGGTCAAAAATGTCTGCCTG

Product: amino acid transporter LysE

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSLDTFLLYLAAILLIAISPGPMAMLSMSHGMHFGKRRSLATALGSVLAALCLMLASAAGLGALLAATEYGFVLLKWCGA
AYLLYLGMRLLLTKPQPSDFKLESPKGKGRRRDLFQQAFLVGISNPKDLLFFAALFPQFIDVSAPQGAQLAILASSWAIV
DFSFVMIYASLASLLAPKLSASNRLHWFDRTSGGVFVSLAALLAIKN

Sequences:

>Translated_207_residues
MSLDTFLLYLAAILLIAISPGPMAMLSMSHGMHFGKRRSLATALGSVLAALCLMLASAAGLGALLAATEYGFVLLKWCGA
AYLLYLGMRLLLTKPQPSDFKLESPKGKGRRRDLFQQAFLVGISNPKDLLFFAALFPQFIDVSAPQGAQLAILASSWAIV
DFSFVMIYASLASLLAPKLSASNRLHWFDRTSGGVFVSLAALLAIKN
>Mature_206_residues
SLDTFLLYLAAILLIAISPGPMAMLSMSHGMHFGKRRSLATALGSVLAALCLMLASAAGLGALLAATEYGFVLLKWCGAA
YLLYLGMRLLLTKPQPSDFKLESPKGKGRRRDLFQQAFLVGISNPKDLLFFAALFPQFIDVSAPQGAQLAILASSWAIVD
FSFVMIYASLASLLAPKLSASNRLHWFDRTSGGVFVSLAALLAIKN

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=204, Percent_Identity=32.3529411764706, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1788099, Length=159, Percent_Identity=31.4465408805031, Blast_Score=84, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22217; Mature: 22086

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDTFLLYLAAILLIAISPGPMAMLSMSHGMHFGKRRSLATALGSVLAALCLMLASAAG
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LGALLAATEYGFVLLKWCGAAYLLYLGMRLLLTKPQPSDFKLESPKGKGRRRDLFQQAFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHH
VGISNPKDLLFFAALFPQFIDVSAPQGAQLAILASSWAIVDFSFVMIYASLASLLAPKLS
HCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
ASNRLHWFDRTSGGVFVSLAALLAIKN
CCCCEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SLDTFLLYLAAILLIAISPGPMAMLSMSHGMHFGKRRSLATALGSVLAALCLMLASAAG
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LGALLAATEYGFVLLKWCGAAYLLYLGMRLLLTKPQPSDFKLESPKGKGRRRDLFQQAFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHH
VGISNPKDLLFFAALFPQFIDVSAPQGAQLAILASSWAIVDFSFVMIYASLASLLAPKLS
HCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
ASNRLHWFDRTSGGVFVSLAALLAIKN
CCCCEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]