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The map label for this gene is pleD [H]

Identifier: 119776669

GI number: 119776669

Start: 4168309

End: 4169490

Strand: Reverse

Name: pleD [H]

Synonym: Sama_3537

Alternate gene names: 119776669

Gene position: 4169490-4168309 (Counterclockwise)

Preceding gene: 119776674

Following gene: 119776655

Centisome position: 96.83

GC content: 54.82

Gene sequence:

>1182_bases
ATGGACATCCAAGCTGAGATCCTCATTGAGGTCATAGTGCTGTTGACGGCCGCCTGTGCCGCAGCCTGGGCTCTGCTGGC
GGGCCCATTACGCATAGCGCCAAAGGCATGTCGCCAGTACGCGGTGGCCAATGGATTGTTGATGTTGGGGCTGATTCTCA
ATGCGTTACGCACGGAGGGGGCGAGCTATATCACTTGGTTCGGCGCCGATTTAAGCCTCATTGCCGGCATCATCATGATG
CGCTTTGGCACAGTATTCCTGTTTCGACTACCAATAAACTACAAGCCTGAGCTGTTGCTGTTGGCTGTGGTGATATTTGC
CATGCTGATGGTACCGCCTTCGGTGGAGTCACAGCATTATCTCGGCATCGTGTTCTCGCTGGCGGCGGCCCTCTTGTTTG
TACGTTTGGGTGAATCAACCTACAAGGGACTTCTGCCCAGTGTCGGCCATAACATCACCTTGCTGCTGGTGATGCCATTG
ATACTGGCGGCTGTGCTGTTTTTAGTGCGGCTCGGTCTTATCATGACCCAGGAAGATGCAGAAGCCCGTTTTATTTCCAT
CCATACCGCTGAAGCTGTGCCCATGCTGTGGTTTTATCTGGTGCTCAGCCTCAGTATCAATGTCGTTGCCATGGGCAATG
CCCTTACCCGCCTGGTGCAAAAAATCAGGGTGCTGGCGGACAGAGACATGCTGACAGGTCTGTGGAATCGTCGCAGCGCA
GAACTGAGGCTAAGAGAGCTCAACCTGCGCTGGCAGAAGGGGCGAGAACCATTTGCCATCGTGTTGCTGGATTTGGATCA
CTTCAAAAAAATCAACGATAAGCTCGGGCATCAGGCCGGTGATGCGGCGCTGCGGCAAGCGGCGCTGATATTAAGCCATA
GTCTCAGAGAAACCGATATGTTATGCCGTTTTGGCGGCGAGGAGTTTCTGGTTATTTTATCCGGTGTCGGCATTAAGCAG
GCACATCTGGTGGCCGAAAAACTGAGAGAGAGTCTGGCTGCAACGGCGCTGCGCTGGGATGACGATGAAGTGGTATTGAG
CGCAAGTTTTGGCTGCGCCGCGATGGTGGATGGGATGAGTGTCTCTCAGCTGATTTCGGCAGCCGACCACGCCATGTATC
AGGCCAAGGCAGGTGGCCGCAACTGCGTGATGGACTCAGCGGCCGGGCCACTCCAGGTCTGA

Upstream 100 bases:

>100_bases
CCGAAAAATGGCAAGACTTGTCGGACAAAAAACTGACATTCTCCCCTTACGACGGCTATAGTAACGCCACTAAAAAAAAA
ATAAAAACTCTCGGGGTTTA

Downstream 100 bases:

>100_bases
TGGATTGCACCAGTCGTTTTTGAGCTGTTGTGATAAGGTGTCTGAACAGAAGGCTTCGCCCACCGAGGGCTTACTTTCAA
AACGGTAATCCCGACTTCGA

Product: GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MDIQAEILIEVIVLLTAACAAAWALLAGPLRIAPKACRQYAVANGLLMLGLILNALRTEGASYITWFGADLSLIAGIIMM
RFGTVFLFRLPINYKPELLLLAVVIFAMLMVPPSVESQHYLGIVFSLAAALLFVRLGESTYKGLLPSVGHNITLLLVMPL
ILAAVLFLVRLGLIMTQEDAEARFISIHTAEAVPMLWFYLVLSLSINVVAMGNALTRLVQKIRVLADRDMLTGLWNRRSA
ELRLRELNLRWQKGREPFAIVLLDLDHFKKINDKLGHQAGDAALRQAALILSHSLRETDMLCRFGGEEFLVILSGVGIKQ
AHLVAEKLRESLAATALRWDDDEVVLSASFGCAAMVDGMSVSQLISAADHAMYQAKAGGRNCVMDSAAGPLQV

Sequences:

>Translated_393_residues
MDIQAEILIEVIVLLTAACAAAWALLAGPLRIAPKACRQYAVANGLLMLGLILNALRTEGASYITWFGADLSLIAGIIMM
RFGTVFLFRLPINYKPELLLLAVVIFAMLMVPPSVESQHYLGIVFSLAAALLFVRLGESTYKGLLPSVGHNITLLLVMPL
ILAAVLFLVRLGLIMTQEDAEARFISIHTAEAVPMLWFYLVLSLSINVVAMGNALTRLVQKIRVLADRDMLTGLWNRRSA
ELRLRELNLRWQKGREPFAIVLLDLDHFKKINDKLGHQAGDAALRQAALILSHSLRETDMLCRFGGEEFLVILSGVGIKQ
AHLVAEKLRESLAATALRWDDDEVVLSASFGCAAMVDGMSVSQLISAADHAMYQAKAGGRNCVMDSAAGPLQV
>Mature_393_residues
MDIQAEILIEVIVLLTAACAAAWALLAGPLRIAPKACRQYAVANGLLMLGLILNALRTEGASYITWFGADLSLIAGIIMM
RFGTVFLFRLPINYKPELLLLAVVIFAMLMVPPSVESQHYLGIVFSLAAALLFVRLGESTYKGLLPSVGHNITLLLVMPL
ILAAVLFLVRLGLIMTQEDAEARFISIHTAEAVPMLWFYLVLSLSINVVAMGNALTRLVQKIRVLADRDMLTGLWNRRSA
ELRLRELNLRWQKGREPFAIVLLDLDHFKKINDKLGHQAGDAALRQAALILSHSLRETDMLCRFGGEEFLVILSGVGIKQ
AHLVAEKLRESLAATALRWDDDEVVLSASFGCAAMVDGMSVSQLISAADHAMYQAKAGGRNCVMDSAAGPLQV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=42.2360248447205, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1787262, Length=271, Percent_Identity=29.1512915129151, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786584, Length=163, Percent_Identity=35.5828220858896, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=34.3949044585987, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788381, Length=168, Percent_Identity=33.9285714285714, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=175, Percent_Identity=29.7142857142857, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=32.1428571428571, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=31.7073170731707, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=36.046511627907, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=29.940119760479, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081974, Length=165, Percent_Identity=31.5151515151515, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42945; Mature: 42945

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIQAEILIEVIVLLTAACAAAWALLAGPLRIAPKACRQYAVANGLLMLGLILNALRTEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASYITWFGADLSLIAGIIMMRFGTVFLFRLPINYKPELLLLAVVIFAMLMVPPSVESQHY
CCEEEEECCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHH
LGIVFSLAAALLFVRLGESTYKGLLPSVGHNITLLLVMPLILAAVLFLVRLGLIMTQEDA
HHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCC
EARFISIHTAEAVPMLWFYLVLSLSINVVAMGNALTRLVQKIRVLADRDMLTGLWNRRSA
CCEEEEEECHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ELRLRELNLRWQKGREPFAIVLLDLDHFKKINDKLGHQAGDAALRQAALILSHSLRETDM
HHHHHHHCCHHHCCCCCEEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LCRFGGEEFLVILSGVGIKQAHLVAEKLRESLAATALRWDDDEVVLSASFGCAAMVDGMS
HHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCC
VSQLISAADHAMYQAKAGGRNCVMDSAAGPLQV
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MDIQAEILIEVIVLLTAACAAAWALLAGPLRIAPKACRQYAVANGLLMLGLILNALRTEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASYITWFGADLSLIAGIIMMRFGTVFLFRLPINYKPELLLLAVVIFAMLMVPPSVESQHY
CCEEEEECCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHH
LGIVFSLAAALLFVRLGESTYKGLLPSVGHNITLLLVMPLILAAVLFLVRLGLIMTQEDA
HHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCC
EARFISIHTAEAVPMLWFYLVLSLSINVVAMGNALTRLVQKIRVLADRDMLTGLWNRRSA
CCEEEEEECHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ELRLRELNLRWQKGREPFAIVLLDLDHFKKINDKLGHQAGDAALRQAALILSHSLRETDM
HHHHHHHCCHHHCCCCCEEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LCRFGGEEFLVILSGVGIKQAHLVAEKLRESLAATALRWDDDEVVLSASFGCAAMVDGMS
HHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCC
VSQLISAADHAMYQAKAGGRNCVMDSAAGPLQV
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA