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The map label for this gene is plsB

Identifier: 119776612

GI number: 119776612

Start: 4111022

End: 4113445

Strand: Reverse

Name: plsB

Synonym: Sama_3480

Alternate gene names: 119776612

Gene position: 4113445-4111022 (Counterclockwise)

Preceding gene: 119776619

Following gene: 119776611

Centisome position: 95.53

GC content: 52.48

Gene sequence:

>2424_bases
ATGTCAGCACACGAGTCTTTTTGGTTTAAGTCCTTGCGGTGGATCCAAAAGCAGTTGGTACACACCATAGTGGTACCTCA
GGATCCCTTCGCCGATTTGAATCTCGATCCCTCCCGCCCCTTGGTTTACGTCATGAAAACCGAGTCTGTGAGCGATATCG
CTGCGCTCCATGAGATAACTGGCAAGCTTGGATTACCCAGCCCCTATCAGATGCTGGAAATTGATGGGATCAAGACCCCT
AGGGTCGTGTGTCTGGAAGGCCGAAAGCCGCTGTTTGGAAAGCGTGACAGTAATGAGCCTTTTTTGCAGACCTTCCAGCA
ATTACTGGCCCTGCACCGTCAGCAGCAAGAACTCGATATACAGCTGGTGCCTGTGAGTCTTTACTGGGGACGTACCCCGG
GTAAAGAGGATGACACCATGCGTGCGGCAGTACTCGAACGGGAAGATCCGACCTGGCTGCGTAAGTGTTTGATGATTTTG
TTCCTGGGTCGTCATAACTTTGTGCAGTTTTCCCGTGCTGTGTCACTTCGTCATATGGCCGATGAACATGGCACCGATAA
GCGCATTGCCCACAAGCTTGCCCGGGTCGCGAGAGTCCATTTCCGCCGTCAGCGTAAGGTGATGACCGGACCTGTGTTGC
CCAATCGTCAAGCCATGTTCCATGCCCTGCTGAAATCTGACAACCTGAAAAAAGCCATTGCTGAAGAGGCCTCCAGTAAG
AAAATCTCAGAAGAAAAGGCCCGTGAAACTGCCATTCAATATTTGGATGAGATTGCTGCCGACTACTCCGACAGCCTGGT
ACGTATCGCCGAGCGCTTCCTCACCTGGCTGTGGAACAAGCTCTATAAGGGCATCAGTATCAAGGGTGCCGAGCAAATTC
GCCAGTTGCACCACGACGGTCATGAGATTGTGTATGTGCCTTGCCACCGCTCGCACATGGATTATTTGTTGCTGTCTTAC
ATCCTTTATTACGAAGGCATGGTGCCGCCCCACATTGCGGCCGGGATTAACCTGAATTTCTGGCCAGCAGGCCCCATGTT
CCGCCGTGGCGGCGCCTTCTTTATCCGCCGCAGCTTCAACGGCAACAAGCTCTACACCGCAGTATTCCGTGAATATCTGG
ATCAGCTGTTCGCAAAGGGGTATTCGGTGGAGTACTTCACCGAAGGTGGCCGCTCCCGTACCGGTCGTCTGCTGGCGCCT
AAAACCGGCATGCTGGCCATGACCTTGTCTTCGGTTATCCGTGGCATTGAGCGACCAGTGACTCTGGTGCCTGTGTATTT
GGGTTACGACCATGTGATGGAAGTGGCTACTTATCACAAAGAGCTGTCGGGCAAGAAAAAAGAAAAGGAATCCGTGTGGC
AGGTATTTGGTGCCATACGCAAACTGGGTAATTTTGGCCGTGGCTATGTGAATTTTGGCCAGCCCATCACCCTGCAGAAC
TTTTTGACCGAAAAAGTTCCCAATTGGCGCGAGGAAGTGGGTGAAGATCCCGAACAAAAACCTTCCTGGCTGACCCCGGT
GGTGAATGCACTGGCCAATCGGGTGATGACCCGTATCAATGATGCCGCAGCCGCCAGTTCTGTTACGCTCTCCAGCATGG
TATTGCTGGCCTCGGAGCAAAATGCGCTGGAGCGCAACCAGCTGGAGCGCCAGATTGATTTGTATCTGAGCCTGCTGAAA
TCCGTCCCTTATACCTCGTACGCATCAGTGACAGAGGGTTGTGGCAAGGAGCTGGTCGATCGCGGCATTGAGCTGAACAA
GCTGACCGAAACCAAGGATGATTTGGGTACCATCATCTCCATTGATGATAGCCTTGCCATCTCCATGACCTATTATCGCA
ACAACATCATCCACTTGTTTGTGATCCCCTCATTGATTGCCACAGTAATGGTGCGCCATGAAGAAGTCTCCAGAGAAGAA
CTGCAGGAGCTGGTTGCCGAGTTCTATCCGCTGCTCAAGGCAGAGCTCTTTATGGGTGTTACCGATTTGCCAGCCTATGT
GGATGCGCTGGTGGAGTGCTTTAAGTCAGAGGGCCTGATTACCGGCGATAACCGACTGAAACTGGTCGACGACCGTATCA
ATCAGCTGTTGTTGCTGGCCGGTGTGGTAGGTGAAACTCTCAAACGCTACGCCATTATTTTCAACCTCTTGGGTGAGCAA
CCCAGAATGGAGCGCGCCGACCTCGAGCATCACAGCCATCGTCTGGCCAGTCGCCTCGGTGCCATTCATGGGGTCATGGC
ACCTGAGTTTTACGACAAAAAGCTCTATGCATTGCTGTCGAGTAAGCTGAAAGATCTGGGTTATCTGTCTGATAAGGCAG
ATGGCGACAAGGTGCGTAAAATCCGTGATCATGCCAACGGGCTCCTCAGAAGCTCAGTGCGTCAGACCATTATTGAGACG
CTGAATCAGGAGCAGGACGACTGA

Upstream 100 bases:

>100_bases
TCAGCGCAAGTATTAACCATTTTTCAGATTATTGGCTAATGGCTGCGGCTGTCTGATATTCTATTGCGCTATTGATTGAA
GCCAGGTTCCCAATATCAGA

Downstream 100 bases:

>100_bases
TGGCTAACCACATGAATGCCGCCAGGCATCCGGTCAAAACTCCATCGCTGCTGGGTGGCGCCATGATTATCGCCGGTACT
ACGGTGGGAGCAGGAATGTT

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MSAHESFWFKSLRWIQKQLVHTIVVPQDPFADLNLDPSRPLVYVMKTESVSDIAALHEITGKLGLPSPYQMLEIDGIKTP
RVVCLEGRKPLFGKRDSNEPFLQTFQQLLALHRQQQELDIQLVPVSLYWGRTPGKEDDTMRAAVLEREDPTWLRKCLMIL
FLGRHNFVQFSRAVSLRHMADEHGTDKRIAHKLARVARVHFRRQRKVMTGPVLPNRQAMFHALLKSDNLKKAIAEEASSK
KISEEKARETAIQYLDEIAADYSDSLVRIAERFLTWLWNKLYKGISIKGAEQIRQLHHDGHEIVYVPCHRSHMDYLLLSY
ILYYEGMVPPHIAAGINLNFWPAGPMFRRGGAFFIRRSFNGNKLYTAVFREYLDQLFAKGYSVEYFTEGGRSRTGRLLAP
KTGMLAMTLSSVIRGIERPVTLVPVYLGYDHVMEVATYHKELSGKKKEKESVWQVFGAIRKLGNFGRGYVNFGQPITLQN
FLTEKVPNWREEVGEDPEQKPSWLTPVVNALANRVMTRINDAAAASSVTLSSMVLLASEQNALERNQLERQIDLYLSLLK
SVPYTSYASVTEGCGKELVDRGIELNKLTETKDDLGTIISIDDSLAISMTYYRNNIIHLFVIPSLIATVMVRHEEVSREE
LQELVAEFYPLLKAELFMGVTDLPAYVDALVECFKSEGLITGDNRLKLVDDRINQLLLLAGVVGETLKRYAIIFNLLGEQ
PRMERADLEHHSHRLASRLGAIHGVMAPEFYDKKLYALLSSKLKDLGYLSDKADGDKVRKIRDHANGLLRSSVRQTIIET
LNQEQDD

Sequences:

>Translated_807_residues
MSAHESFWFKSLRWIQKQLVHTIVVPQDPFADLNLDPSRPLVYVMKTESVSDIAALHEITGKLGLPSPYQMLEIDGIKTP
RVVCLEGRKPLFGKRDSNEPFLQTFQQLLALHRQQQELDIQLVPVSLYWGRTPGKEDDTMRAAVLEREDPTWLRKCLMIL
FLGRHNFVQFSRAVSLRHMADEHGTDKRIAHKLARVARVHFRRQRKVMTGPVLPNRQAMFHALLKSDNLKKAIAEEASSK
KISEEKARETAIQYLDEIAADYSDSLVRIAERFLTWLWNKLYKGISIKGAEQIRQLHHDGHEIVYVPCHRSHMDYLLLSY
ILYYEGMVPPHIAAGINLNFWPAGPMFRRGGAFFIRRSFNGNKLYTAVFREYLDQLFAKGYSVEYFTEGGRSRTGRLLAP
KTGMLAMTLSSVIRGIERPVTLVPVYLGYDHVMEVATYHKELSGKKKEKESVWQVFGAIRKLGNFGRGYVNFGQPITLQN
FLTEKVPNWREEVGEDPEQKPSWLTPVVNALANRVMTRINDAAAASSVTLSSMVLLASEQNALERNQLERQIDLYLSLLK
SVPYTSYASVTEGCGKELVDRGIELNKLTETKDDLGTIISIDDSLAISMTYYRNNIIHLFVIPSLIATVMVRHEEVSREE
LQELVAEFYPLLKAELFMGVTDLPAYVDALVECFKSEGLITGDNRLKLVDDRINQLLLLAGVVGETLKRYAIIFNLLGEQ
PRMERADLEHHSHRLASRLGAIHGVMAPEFYDKKLYALLSSKLKDLGYLSDKADGDKVRKIRDHANGLLRSSVRQTIIET
LNQEQDD
>Mature_806_residues
SAHESFWFKSLRWIQKQLVHTIVVPQDPFADLNLDPSRPLVYVMKTESVSDIAALHEITGKLGLPSPYQMLEIDGIKTPR
VVCLEGRKPLFGKRDSNEPFLQTFQQLLALHRQQQELDIQLVPVSLYWGRTPGKEDDTMRAAVLEREDPTWLRKCLMILF
LGRHNFVQFSRAVSLRHMADEHGTDKRIAHKLARVARVHFRRQRKVMTGPVLPNRQAMFHALLKSDNLKKAIAEEASSKK
ISEEKARETAIQYLDEIAADYSDSLVRIAERFLTWLWNKLYKGISIKGAEQIRQLHHDGHEIVYVPCHRSHMDYLLLSYI
LYYEGMVPPHIAAGINLNFWPAGPMFRRGGAFFIRRSFNGNKLYTAVFREYLDQLFAKGYSVEYFTEGGRSRTGRLLAPK
TGMLAMTLSSVIRGIERPVTLVPVYLGYDHVMEVATYHKELSGKKKEKESVWQVFGAIRKLGNFGRGYVNFGQPITLQNF
LTEKVPNWREEVGEDPEQKPSWLTPVVNALANRVMTRINDAAAASSVTLSSMVLLASEQNALERNQLERQIDLYLSLLKS
VPYTSYASVTEGCGKELVDRGIELNKLTETKDDLGTIISIDDSLAISMTYYRNNIIHLFVIPSLIATVMVRHEEVSREEL
QELVAEFYPLLKAELFMGVTDLPAYVDALVECFKSEGLITGDNRLKLVDDRINQLLLLAGVVGETLKRYAIIFNLLGEQP
RMERADLEHHSHRLASRLGAIHGVMAPEFYDKKLYALLSSKLKDLGYLSDKADGDKVRKIRDHANGLLRSSVRQTIIETL
NQEQDD

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family

Homologues:

Organism=Homo sapiens, GI7657134, Length=264, Percent_Identity=34.0909090909091, Blast_Score=152, Evalue=2e-36,
Organism=Homo sapiens, GI190358539, Length=490, Percent_Identity=25.1020408163265, Blast_Score=137, Evalue=5e-32,
Organism=Escherichia coli, GI87082362, Length=802, Percent_Identity=49.2518703241895, Blast_Score=775, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147672, Length=583, Percent_Identity=26.4150943396226, Blast_Score=160, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71988723, Length=449, Percent_Identity=28.0623608017817, Blast_Score=152, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI71988728, Length=388, Percent_Identity=29.1237113402062, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17864692, Length=325, Percent_Identity=31.3846153846154, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24650754, Length=490, Percent_Identity=26.530612244898, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI21357731, Length=498, Percent_Identity=26.3052208835341, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24650752, Length=490, Percent_Identity=26.530612244898, Blast_Score=135, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSB_SHEAM (A1SBC6)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_929352.1
- ProteinModelPortal:   A1SBC6
- STRING:   A1SBC6
- GeneID:   4605727
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_3480
- NMPDR:   fig|326297.7.peg.3279
- eggNOG:   COG2937
- HOGENOM:   HBG296590
- OMA:   VIYVPCH
- ProtClustDB:   PRK04974
- HAMAP:   MF_00393
- InterPro:   IPR002123
- InterPro:   IPR022284
- SMART:   SM00563
- TIGRFAMs:   TIGR03703

Pfam domain/function: PF01553 Acyltransferase

EC number: =2.3.1.15

Molecular weight: Translated: 92012; Mature: 91881

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHESFWFKSLRWIQKQLVHTIVVPQDPFADLNLDPSRPLVYVMKTESVSDIAALHEIT
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
GKLGLPSPYQMLEIDGIKTPRVVCLEGRKPLFGKRDSNEPFLQTFQQLLALHRQQQELDI
HCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCE
QLVPVSLYWGRTPGKEDDTMRAAVLEREDPTWLRKCLMILFLGRHNFVQFSRAVSLRHMA
EEEEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DEHGTDKRIAHKLARVARVHFRRQRKVMTGPVLPNRQAMFHALLKSDNLKKAIAEEASSK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
KISEEKARETAIQYLDEIAADYSDSLVRIAERFLTWLWNKLYKGISIKGAEQIRQLHHDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
HEIVYVPCHRSHMDYLLLSYILYYEGMVPPHIAAGINLNFWPAGPMFRRGGAFFIRRSFN
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEECCCCCHHHCCCEEEEEECCC
GNKLYTAVFREYLDQLFAKGYSVEYFTEGGRSRTGRLLAPKTGMLAMTLSSVIRGIERPV
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCC
TLVPVYLGYDHVMEVATYHKELSGKKKEKESVWQVFGAIRKLGNFGRGYVNFGQPITLQN
EEEEEHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHH
FLTEKVPNWREEVGEDPEQKPSWLTPVVNALANRVMTRINDAAAASSVTLSSMVLLASEQ
HHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
NALERNQLERQIDLYLSLLKSVPYTSYASVTEGCGKELVDRGIELNKLTETKDDLGTIIS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHCCCEEE
IDDSLAISMTYYRNNIIHLFVIPSLIATVMVRHEEVSREELQELVAEFYPLLKAELFMGV
ECCCEEEEEEEHHCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCH
TDLPAYVDALVECFKSEGLITGDNRLKLVDDRINQLLLLAGVVGETLKRYAIIFNLLGEQ
HHHHHHHHHHHHHHHCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PRMERADLEHHSHRLASRLGAIHGVMAPEFYDKKLYALLSSKLKDLGYLSDKADGDKVRK
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
IRDHANGLLRSSVRQTIIETLNQEQDD
HHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SAHESFWFKSLRWIQKQLVHTIVVPQDPFADLNLDPSRPLVYVMKTESVSDIAALHEIT
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
GKLGLPSPYQMLEIDGIKTPRVVCLEGRKPLFGKRDSNEPFLQTFQQLLALHRQQQELDI
HCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCE
QLVPVSLYWGRTPGKEDDTMRAAVLEREDPTWLRKCLMILFLGRHNFVQFSRAVSLRHMA
EEEEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DEHGTDKRIAHKLARVARVHFRRQRKVMTGPVLPNRQAMFHALLKSDNLKKAIAEEASSK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
KISEEKARETAIQYLDEIAADYSDSLVRIAERFLTWLWNKLYKGISIKGAEQIRQLHHDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
HEIVYVPCHRSHMDYLLLSYILYYEGMVPPHIAAGINLNFWPAGPMFRRGGAFFIRRSFN
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEECCCCCHHHCCCEEEEEECCC
GNKLYTAVFREYLDQLFAKGYSVEYFTEGGRSRTGRLLAPKTGMLAMTLSSVIRGIERPV
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCC
TLVPVYLGYDHVMEVATYHKELSGKKKEKESVWQVFGAIRKLGNFGRGYVNFGQPITLQN
EEEEEHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHH
FLTEKVPNWREEVGEDPEQKPSWLTPVVNALANRVMTRINDAAAASSVTLSSMVLLASEQ
HHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
NALERNQLERQIDLYLSLLKSVPYTSYASVTEGCGKELVDRGIELNKLTETKDDLGTIIS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHCCCEEE
IDDSLAISMTYYRNNIIHLFVIPSLIATVMVRHEEVSREELQELVAEFYPLLKAELFMGV
ECCCEEEEEEEHHCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCH
TDLPAYVDALVECFKSEGLITGDNRLKLVDDRINQLLLLAGVVGETLKRYAIIFNLLGEQ
HHHHHHHHHHHHHHHCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PRMERADLEHHSHRLASRLGAIHGVMAPEFYDKKLYALLSSKLKDLGYLSDKADGDKVRK
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
IRDHANGLLRSSVRQTIIETLNQEQDD
HHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA