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The map label for this gene is ycbU [H]

Identifier: 119776549

GI number: 119776549

Start: 4046290

End: 4047405

Strand: Reverse

Name: ycbU [H]

Synonym: Sama_3417

Alternate gene names: 119776549

Gene position: 4047405-4046290 (Counterclockwise)

Preceding gene: 119776550

Following gene: 119776547

Centisome position: 93.99

GC content: 56.09

Gene sequence:

>1116_bases
ATGAGTGACCATAAATCAGCATTTTTTCTACCCGATGGCACCTATCTGCTGAGCCACTCGGTTGGCAGACCGCTCAAATC
TGCCAAGGAAGGATTCGAGCAGGATTATTGGCAGCCATGGTGTCAATCGGGGCGAGAGCCCTGGGGTGACTGGTTGTCTG
GCGTTGAAGCGTTTCGCACGGCGTTGGGACAACTCTTTGATGGTAACGCCAGGGATTTTTGTCCACAGGTGAATTTGTCG
TCGGCACTGACCAAGTTGGTGTTATCGCATCCAAGGCTTAACGTGCCGGGCGCGCGCGTTCTGATGTGCGAGGAAGACTT
CCCGTCCATGGGGTTTGCGCTGCAGCGGGCCTTGCCCCATGCCCGTCTGGAATTCATTCCAGCGGGTATCGATATTACAG
ATGCCAATGCCTGGGAGGCGTATTTAACCCCGGGACTGGATCTGGTGTTTATCAGCCATGCGTATTCCAACCTTGGAGCC
TTGTCACCAGTGGAAGCGCTGGTTCCGCGTTGTCGTGAGCTGGGGAGCTTGACTCTGGTGGATGTGGCCCAGTCGGCGGG
GGCCGTGCCCTTATCACTGGCTCAGCTGGCACCTGACTTTCTGATTGGCTCGGCGGTGAAGTGGCTCTGCTCCGGGCCGG
GCGCTGCCTACCTGTGGGTGAATCCCGAAAGGCTTGCGGAATGCGAGCCTGTGGATGTGGGTTGGTTTTCCCATGAAAAC
CCCTTTGAGTTCAATATTCACGACTTTCGCTACCATCCGTCGGCGCTGCGATTTTGGGGTGGGACCCCCTCTGTGGCGCC
TTATGTGATTGGCGCACACAGCATTGGCTGGTTTGCGACCCTGGGTGCCGACAAGGTGAGAGCGCATAATCAGGCTTTGG
TTTCAATGCTTTATAATGAGCTGCACCAGTATGGTGCAGGCCCTTTGCAGGCACAAAGCCGCAGCGGTACTGCCATTTTG
CAATTCGGAGACAGCACTGCAGCGGTGATGGCCGCCCTCAATGATGCCGGTATCAGCGTCGACAATCGTCGCTACGGTAT
GCGCGTATCACCGCATCTCTATAACGATGAGGCCGATATACAGCGTTTTATTGAGTGCGTACGAGGCGCACTGTAA

Upstream 100 bases:

>100_bases
TTGGTGCGCTGGAAAAACTCAGGGATAGACGCGCCGCGGCAAAGCGCAGCGATATACGCACTGCCTGGCAGGATGTGGCG
CGACTGAAGGCGACCTTGTT

Downstream 100 bases:

>100_bases
GGCGCATGGCACGGTGGAGGCGACATAGTTGTCTTGGTTGTCATAAGCCCAAAAAAGACGCCCCGGACCTGAGTCCGGGG
CGTCTTTTTTGTTTTCCGCC

Product: kynureninase

Products: L-alanine; S [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSDHKSAFFLPDGTYLLSHSVGRPLKSAKEGFEQDYWQPWCQSGREPWGDWLSGVEAFRTALGQLFDGNARDFCPQVNLS
SALTKLVLSHPRLNVPGARVLMCEEDFPSMGFALQRALPHARLEFIPAGIDITDANAWEAYLTPGLDLVFISHAYSNLGA
LSPVEALVPRCRELGSLTLVDVAQSAGAVPLSLAQLAPDFLIGSAVKWLCSGPGAAYLWVNPERLAECEPVDVGWFSHEN
PFEFNIHDFRYHPSALRFWGGTPSVAPYVIGAHSIGWFATLGADKVRAHNQALVSMLYNELHQYGAGPLQAQSRSGTAIL
QFGDSTAAVMAALNDAGISVDNRRYGMRVSPHLYNDEADIQRFIECVRGAL

Sequences:

>Translated_371_residues
MSDHKSAFFLPDGTYLLSHSVGRPLKSAKEGFEQDYWQPWCQSGREPWGDWLSGVEAFRTALGQLFDGNARDFCPQVNLS
SALTKLVLSHPRLNVPGARVLMCEEDFPSMGFALQRALPHARLEFIPAGIDITDANAWEAYLTPGLDLVFISHAYSNLGA
LSPVEALVPRCRELGSLTLVDVAQSAGAVPLSLAQLAPDFLIGSAVKWLCSGPGAAYLWVNPERLAECEPVDVGWFSHEN
PFEFNIHDFRYHPSALRFWGGTPSVAPYVIGAHSIGWFATLGADKVRAHNQALVSMLYNELHQYGAGPLQAQSRSGTAIL
QFGDSTAAVMAALNDAGISVDNRRYGMRVSPHLYNDEADIQRFIECVRGAL
>Mature_370_residues
SDHKSAFFLPDGTYLLSHSVGRPLKSAKEGFEQDYWQPWCQSGREPWGDWLSGVEAFRTALGQLFDGNARDFCPQVNLSS
ALTKLVLSHPRLNVPGARVLMCEEDFPSMGFALQRALPHARLEFIPAGIDITDANAWEAYLTPGLDLVFISHAYSNLGAL
SPVEALVPRCRELGSLTLVDVAQSAGAVPLSLAQLAPDFLIGSAVKWLCSGPGAAYLWVNPERLAECEPVDVGWFSHENP
FEFNIHDFRYHPSALRFWGGTPSVAPYVIGAHSIGWFATLGADKVRAHNQALVSMLYNELHQYGAGPLQAQSRSGTAILQ
FGDSTAAVMAALNDAGISVDNRRYGMRVSPHLYNDEADIQRFIECVRGAL

Specific function: Fe-S cluster biosynthesis [C]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: NA

Molecular weight: Translated: 40604; Mature: 40473

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHKSAFFLPDGTYLLSHSVGRPLKSAKEGFEQDYWQPWCQSGREPWGDWLSGVEAFRT
CCCCCCEEECCCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ALGQLFDGNARDFCPQVNLSSALTKLVLSHPRLNVPGARVLMCEEDFPSMGFALQRALPH
HHHHHHCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCC
ARLEFIPAGIDITDANAWEAYLTPGLDLVFISHAYSNLGALSPVEALVPRCRELGSLTLV
HHHEEEECCCEEECCCCCHHHHCCCCEEEEHHHHHHHCCCCCHHHHHHHHHHHHCCEEEE
DVAQSAGAVPLSLAQLAPDFLIGSAVKWLCSGPGAAYLWVNPERLAECEPVDVGWFSHEN
HHHHHCCCCCHHHHHHCHHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCCEECCCCCCC
PFEFNIHDFRYHPSALRFWGGTPSVAPYVIGAHSIGWFATLGADKVRAHNQALVSMLYNE
CEEECCCCEEECCCHHEECCCCCCCCCEEEECCCCCHHEECCHHHHHHHHHHHHHHHHHH
LHQYGAGPLQAQSRSGTAILQFGDSTAAVMAALNDAGISVDNRRYGMRVSPHLYNDEADI
HHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHHH
QRFIECVRGAL
HHHHHHHHCCC
>Mature Secondary Structure 
SDHKSAFFLPDGTYLLSHSVGRPLKSAKEGFEQDYWQPWCQSGREPWGDWLSGVEAFRT
CCCCCEEECCCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ALGQLFDGNARDFCPQVNLSSALTKLVLSHPRLNVPGARVLMCEEDFPSMGFALQRALPH
HHHHHHCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCC
ARLEFIPAGIDITDANAWEAYLTPGLDLVFISHAYSNLGALSPVEALVPRCRELGSLTLV
HHHEEEECCCEEECCCCCHHHHCCCCEEEEHHHHHHHCCCCCHHHHHHHHHHHHCCEEEE
DVAQSAGAVPLSLAQLAPDFLIGSAVKWLCSGPGAAYLWVNPERLAECEPVDVGWFSHEN
HHHHHCCCCCHHHHHHCHHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCCEECCCCCCC
PFEFNIHDFRYHPSALRFWGGTPSVAPYVIGAHSIGWFATLGADKVRAHNQALVSMLYNE
CEEECCCCEEECCCHHEECCCCCCCCCEEEECCCCCHHEECCHHHHHHHHHHHHHHHHHH
LHQYGAGPLQAQSRSGTAILQFGDSTAAVMAALNDAGISVDNRRYGMRVSPHLYNDEADI
HHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHHH
QRFIECVRGAL
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cysteine [C]

Specific reaction: L-cysteine = L-alanine + S [C]

General reaction: transfers sulfane sulfur to ThiI during in vitro biosynthesis of 4-thiouridine in E. coli tRNA [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377; 7704254; 1353026 [H]