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The map label for this gene is glnA [H]

Identifier: 119776548

GI number: 119776548

Start: 4044735

End: 4046144

Strand: Direct

Name: glnA [H]

Synonym: Sama_3416

Alternate gene names: 119776548

Gene position: 4044735-4046144 (Clockwise)

Preceding gene: 119776546

Following gene: 119776551

Centisome position: 93.93

GC content: 54.96

Gene sequence:

>1410_bases
ATGTCAGTTGAAAATGTGTTGAAGCAGTTGAAAGACCTGGAAGTGAAGTTCGTGGATCTGCGCTTTACCGATACCAAGGG
TAAAGAACAGCACGTGTCTATTCCAACGCATCAGGTAGACGCCGACTTCTTTGAAGACGGTAAAATGTTCGACGGCTCAT
CCATCGCCGGTTGGAAAGGTATCAACGAATCTGACATGGTACTGATGCCAGATGCGACCACCTTCGTGCTGGATCCTTTC
ACCGAAGAAACCACTGCCATCATCCGCTGTGACATCCTCGAGCCAGGTACCATGACCGGTTACGACCGTGACCCACGCTC
CATCGCCAAGAAGGCCGAAGAATACCTGCGCGCCACCGGCATCGCCGACACAGTGCTGATGGGTCCTGAGCCAGAATTCT
TCCTGTTCGACGACGTACGTTTCGGCACCGACATGAGCGGCACCTTCGTGAAGATCGACGCCAAAGAAGCCGCCTGGAAC
TCAAGCACCAGCTACGAAGATGGCAACACCGGTCACCGTCCATTCGTGAAAGGCGGTTACTTCCCGGTTGCTCCGGTTGA
CTCTTCTCAGGACCTGCGCTCTGCCATGTGTCTGGTACTGGAAGAAATGGGTCAGGTAGTTGAAGCCCACCACCACGAAG
TGGCCACTGCCGGTCAGAACGAAATCGCCACCCGTTTCAATACCCTGACCAAGAAAGCCGACGAAATCCAGATCCTGAAG
TACGTGGTGCACAACATGGCCCACGCTTACGGCAAGACTGCTACCTTTATGCCTAAGCCAATCGTTGGCGACAACGGATC
TGGTATGCACGTTCACCAGTCTCTGGCCAAAGATGGCGTTAACCTGTTTGCTGGCGACAAGTACGGCGGTCTGTCTGAAA
CTGCCCTGTACTACATTGGCGGTATCATCAAGCACGCCCGCGCCCTGAACGCTTTCACCAACCCAAGCACCAACTCTTAC
AAGCGTCTGGTACCACACTTCGAAGCCCCGGTAATGCTGGCTTACTCAGCCCGTAACCGCTCTGCTTCTATCCGTATCCC
AGTGGTACCAAGCCCTAAAGGCCGTCGTATCGAGACCCGCTTCCCAGACCCAACTGCCAACCCATACCTGGGTTTCGCGG
CTCTGCTGATGGCTGGTTTGGACGGTATCCAGAACAAGATCCACCCTGGCGATGCCATGGATAAGGACCTGTACGATCTG
CCAGCCGAAGAAGCGGCCGAGATCCCAACTGTAGCCGAGTCTCTGGACGTGGCACTGGCGGCTCTGGATGCAGACCGCGA
GTTCCTGACCAAGGGTGGCGTATTCAGCAACGACTTCATCGACTCTTACATCCGTCTGAAGACTGCTGAAGCCGAGCGCG
TGAACCGCACCACTCACCCACTCGAGTTCGAAATGTATTACAGCCTGTAA

Upstream 100 bases:

>100_bases
GGCTTTATCTGTGCCAAGTTTCGGTGCAGCCTGCAAAAGCCACCACCGACAAAAGTAATGATAGTAAGGGAAGACCCTTT
ACCCTACCGGAGACTCAAGC

Downstream 100 bases:

>100_bases
TACCCTGCAAAACCGTCCCTTTATCTGCGGTTAGCAGATAAAGTTGGCGGAAAACAAAAAAGACGCCCCGGACTCAGGTC
CGGGGCGTCTTTTTTGGGCT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSVENVLKQLKDLEVKFVDLRFTDTKGKEQHVSIPTHQVDADFFEDGKMFDGSSIAGWKGINESDMVLMPDATTFVLDPF
TEETTAIIRCDILEPGTMTGYDRDPRSIAKKAEEYLRATGIADTVLMGPEPEFFLFDDVRFGTDMSGTFVKIDAKEAAWN
SSTSYEDGNTGHRPFVKGGYFPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILK
YVVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSETALYYIGGIIKHARALNAFTNPSTNSY
KRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIETRFPDPTANPYLGFAALLMAGLDGIQNKIHPGDAMDKDLYDL
PAEEAAEIPTVAESLDVALAALDADREFLTKGGVFSNDFIDSYIRLKTAEAERVNRTTHPLEFEMYYSL

Sequences:

>Translated_469_residues
MSVENVLKQLKDLEVKFVDLRFTDTKGKEQHVSIPTHQVDADFFEDGKMFDGSSIAGWKGINESDMVLMPDATTFVLDPF
TEETTAIIRCDILEPGTMTGYDRDPRSIAKKAEEYLRATGIADTVLMGPEPEFFLFDDVRFGTDMSGTFVKIDAKEAAWN
SSTSYEDGNTGHRPFVKGGYFPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILK
YVVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSETALYYIGGIIKHARALNAFTNPSTNSY
KRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIETRFPDPTANPYLGFAALLMAGLDGIQNKIHPGDAMDKDLYDL
PAEEAAEIPTVAESLDVALAALDADREFLTKGGVFSNDFIDSYIRLKTAEAERVNRTTHPLEFEMYYSL
>Mature_468_residues
SVENVLKQLKDLEVKFVDLRFTDTKGKEQHVSIPTHQVDADFFEDGKMFDGSSIAGWKGINESDMVLMPDATTFVLDPFT
EETTAIIRCDILEPGTMTGYDRDPRSIAKKAEEYLRATGIADTVLMGPEPEFFLFDDVRFGTDMSGTFVKIDAKEAAWNS
STSYEDGNTGHRPFVKGGYFPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILKY
VVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSETALYYIGGIIKHARALNAFTNPSTNSYK
RLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIETRFPDPTANPYLGFAALLMAGLDGIQNKIHPGDAMDKDLYDLP
AEEAAEIPTVAESLDVALAALDADREFLTKGGVFSNDFIDSYIRLKTAEAERVNRTTHPLEFEMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=77.3987206823028, Blast_Score=772, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=366, Percent_Identity=26.2295081967213, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51773; Mature: 51642

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVENVLKQLKDLEVKFVDLRFTDTKGKEQHVSIPTHQVDADFFEDGKMFDGSSIAGWKG
CCHHHHHHHHHCCCEEEEEEEEECCCCCCCEECCCCCCCCCHHHCCCCEECCCCCCCCCC
INESDMVLMPDATTFVLDPFTEETTAIIRCDILEPGTMTGYDRDPRSIAKKAEEYLRATG
CCCCCEEEECCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
IADTVLMGPEPEFFLFDDVRFGTDMSGTFVKIDAKEAAWNSSTSYEDGNTGHRPFVKGGY
CCCEEEECCCCCEEEEECEECCCCCCCCEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILK
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YVVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSETALYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHH
GIIKHARALNAFTNPSTNSYKRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIETR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEECC
FPDPTANPYLGFAALLMAGLDGIQNKIHPGDAMDKDLYDLPAEEAAEIPTVAESLDVALA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHCCCHHHHHHHHHHH
ALDADREFLTKGGVFSNDFIDSYIRLKTAEAERVNRTTHPLEFEMYYSL
HHCCCHHHHHCCCCCCHHHHHHHHEEECCHHHHHCCCCCCEEEEEEECC
>Mature Secondary Structure 
SVENVLKQLKDLEVKFVDLRFTDTKGKEQHVSIPTHQVDADFFEDGKMFDGSSIAGWKG
CHHHHHHHHHCCCEEEEEEEEECCCCCCCEECCCCCCCCCHHHCCCCEECCCCCCCCCC
INESDMVLMPDATTFVLDPFTEETTAIIRCDILEPGTMTGYDRDPRSIAKKAEEYLRATG
CCCCCEEEECCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
IADTVLMGPEPEFFLFDDVRFGTDMSGTFVKIDAKEAAWNSSTSYEDGNTGHRPFVKGGY
CCCEEEECCCCCEEEEECEECCCCCCCCEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDLRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTKKADEIQILK
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YVVHNMAHAYGKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSETALYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHH
GIIKHARALNAFTNPSTNSYKRLVPHFEAPVMLAYSARNRSASIRIPVVPSPKGRRIETR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEECC
FPDPTANPYLGFAALLMAGLDGIQNKIHPGDAMDKDLYDLPAEEAAEIPTVAESLDVALA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHCCCHHHHHHHHHHH
ALDADREFLTKGGVFSNDFIDSYIRLKTAEAERVNRTTHPLEFEMYYSL
HHCCCHHHHHCCCCCCHHHHHHHHEEECCHHHHHCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]