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The map label for this gene is ykoW [H]

Identifier: 119776540

GI number: 119776540

Start: 4033138

End: 4034487

Strand: Reverse

Name: ykoW [H]

Synonym: Sama_3408

Alternate gene names: 119776540

Gene position: 4034487-4033138 (Counterclockwise)

Preceding gene: 119776547

Following gene: 119776539

Centisome position: 93.69

GC content: 52.07

Gene sequence:

>1350_bases
ATGCAGAGTACCCATTTAGCCCGTACCAAACTCTGGATTGTGGCGTTCGCCATTGTGTACTGGTTCCTGTCATTGTTTAC
GGTGGAATATTTACACCGCAGTTACTTAAGGGATTATCTGGCTGAACAACAACAATGGTTTTCGCAAAAGTTGGCCCTTA
TCCGGGCCAACATCGAGGCCAAGGTGAATGCCGACATTTTGTTGGCAGACAGTCTTGCAACCATTTTGAGCTTTAATCCC
GACGTCCCCTACAGCAAATGGACTTTGCTCGCAGGCCAACTGGCCAGCAAGGGTGAATATATTCGCAGCATCAGTATTGC
CCCCAATGATGTGGTTGCTCTGGTGTATCCGCTGGCCGGTAATGAGTCTGTGATGGGGCTTGATTTTCGGGAAACCCCGG
CGCAGATGCCGGCGGTGCTGGAGGCGAGGATGTCCCGACAAATCACCCTCGATGGTCCCATCACACTGATCCAGGGGGGA
ATTGGCTTGATTGCCCGTGCGCCTGTGTTTGCCGACCCGCCCACCAATACCCAATACTGGGGCGTTTGCAGCATAGTGAT
AGATCTCGACCGCCTGATAAACAGTATTCGGGACGTTGATCTGGGTGAAGGCATGACGCTGGGCATTCGGACGCTGCTGT
ATCCCGATAAACGTGGTCCCATATTCATTGGGCAAAGCAGCGACTTCGACAATGCGCGGGCTGTTGCTCAGGTACATTTA
CTGGGGGCCACCTGGGAGATTGCGCTGGCCGAGCCAGCAGAGTTAAGGTCCTGGGGTGCAACCAATGTGGTGCGTCTCAT
CGGATACTGTGTAACCCTGTTTATCTTCCTGAGCTTTCTGATGGTTTCCAATGCCTACCGTATGGCCCGTCTGGTGTCAT
TGCAGGATGTGCTCACAGGTTTACCAAACAGGCGATTTGCTATGTCGCTGCTCAATAAGCTTATTCAGGCACCTAACGGT
CGTTTTACCGTTATTAACGTGGATCTCAATTATTTCAAGCAGGTGAATGATAGTTTTGGTCATGCCGCCGGCGATGCCAT
GTTAAAGGCGGTCGCCAAACGCATGCAGTCAGTGCTGCGAAACAGCGACATGGTTGCCCGATTGGGAGGTGATGAATTTT
TACTGATTTTGCCCCGATTAACCTCTCCCGCTGATATCAGCCATGTACTGATGAAGGTTCGCCATGCGGCCTGCGAGCAG
CCGCTGCAAATGGCAGACATCACCCTCAATATCAGTATCAGTATGGGGGCGGCCCGGTATCCTGAGGATGGACAGGATAT
CACTGAATTGCTGCATGTTGCCGACAAGGCCATGTACGAAGACAAGCAGCGCATCAAAGGGGAAAGATGA

Upstream 100 bases:

>100_bases
TTGGACGGCAAAACCGGTTGGTACTTTTACAGCTTGGGGTTTCTCAGGATGCCGATATACAGATATGCTTTAGGTAAGCC
TTTCTATTGAAACGTGGTTT

Downstream 100 bases:

>100_bases
TGAAGCTGCAAAGGCAATTCCTTGCTGCATTGTTGTTGTCGCTGAGTTTGCCCAGTACAGCCACAGAGCCACTGACGGTA
CGCTATGTGGATGCCAGCGT

Product: GGDEF family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MQSTHLARTKLWIVAFAIVYWFLSLFTVEYLHRSYLRDYLAEQQQWFSQKLALIRANIEAKVNADILLADSLATILSFNP
DVPYSKWTLLAGQLASKGEYIRSISIAPNDVVALVYPLAGNESVMGLDFRETPAQMPAVLEARMSRQITLDGPITLIQGG
IGLIARAPVFADPPTNTQYWGVCSIVIDLDRLINSIRDVDLGEGMTLGIRTLLYPDKRGPIFIGQSSDFDNARAVAQVHL
LGATWEIALAEPAELRSWGATNVVRLIGYCVTLFIFLSFLMVSNAYRMARLVSLQDVLTGLPNRRFAMSLLNKLIQAPNG
RFTVINVDLNYFKQVNDSFGHAAGDAMLKAVAKRMQSVLRNSDMVARLGGDEFLLILPRLTSPADISHVLMKVRHAACEQ
PLQMADITLNISISMGAARYPEDGQDITELLHVADKAMYEDKQRIKGER

Sequences:

>Translated_449_residues
MQSTHLARTKLWIVAFAIVYWFLSLFTVEYLHRSYLRDYLAEQQQWFSQKLALIRANIEAKVNADILLADSLATILSFNP
DVPYSKWTLLAGQLASKGEYIRSISIAPNDVVALVYPLAGNESVMGLDFRETPAQMPAVLEARMSRQITLDGPITLIQGG
IGLIARAPVFADPPTNTQYWGVCSIVIDLDRLINSIRDVDLGEGMTLGIRTLLYPDKRGPIFIGQSSDFDNARAVAQVHL
LGATWEIALAEPAELRSWGATNVVRLIGYCVTLFIFLSFLMVSNAYRMARLVSLQDVLTGLPNRRFAMSLLNKLIQAPNG
RFTVINVDLNYFKQVNDSFGHAAGDAMLKAVAKRMQSVLRNSDMVARLGGDEFLLILPRLTSPADISHVLMKVRHAACEQ
PLQMADITLNISISMGAARYPEDGQDITELLHVADKAMYEDKQRIKGER
>Mature_449_residues
MQSTHLARTKLWIVAFAIVYWFLSLFTVEYLHRSYLRDYLAEQQQWFSQKLALIRANIEAKVNADILLADSLATILSFNP
DVPYSKWTLLAGQLASKGEYIRSISIAPNDVVALVYPLAGNESVMGLDFRETPAQMPAVLEARMSRQITLDGPITLIQGG
IGLIARAPVFADPPTNTQYWGVCSIVIDLDRLINSIRDVDLGEGMTLGIRTLLYPDKRGPIFIGQSSDFDNARAVAQVHL
LGATWEIALAEPAELRSWGATNVVRLIGYCVTLFIFLSFLMVSNAYRMARLVSLQDVLTGLPNRRFAMSLLNKLIQAPNG
RFTVINVDLNYFKQVNDSFGHAAGDAMLKAVAKRMQSVLRNSDMVARLGGDEFLLILPRLTSPADISHVLMKVRHAACEQ
PLQMADITLNISISMGAARYPEDGQDITELLHVADKAMYEDKQRIKGER

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG3452

COG function: function code T; Predicted periplasmic ligand-binding sensor domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=163, Percent_Identity=35.5828220858896, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87082007, Length=173, Percent_Identity=38.150289017341, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87081977, Length=213, Percent_Identity=30.9859154929577, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=35.8974358974359, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=34.3023255813954, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787802, Length=127, Percent_Identity=33.0708661417323, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787816, Length=172, Percent_Identity=31.3953488372093, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787262, Length=193, Percent_Identity=26.4248704663212, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1786584, Length=160, Percent_Identity=30, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081881, Length=155, Percent_Identity=30.9677419354839, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=175, Percent_Identity=29.7142857142857, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081974, Length=174, Percent_Identity=31.0344827586207, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=163, Percent_Identity=29.4478527607362, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 49976; Mature: 49976

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50839 CHASE ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSTHLARTKLWIVAFAIVYWFLSLFTVEYLHRSYLRDYLAEQQQWFSQKLALIRANIEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
KVNADILLADSLATILSFNPDVPYSKWTLLAGQLASKGEYIRSISIAPNDVVALVYPLAG
ECCCCEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHEEEECCCCCEEEEEEEECC
NESVMGLDFRETPAQMPAVLEARMSRQITLDGPITLIQGGIGLIARAPVFADPPTNTQYW
CCCEEECCCCCCCHHHHHHHHHHHCCEEEECCCEEEEECCCHHHCCCCCCCCCCCCCCHH
GVCSIVIDLDRLINSIRDVDLGEGMTLGIRTLLYPDKRGPIFIGQSSDFDNARAVAQVHL
HHHHHHHHHHHHHHHHHCCCCCCCCEEHHHEEECCCCCCCEEEECCCCCCCHHHHHEEEE
LGATWEIALAEPAELRSWGATNVVRLIGYCVTLFIFLSFLMVSNAYRMARLVSLQDVLTG
ECCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LPNRRFAMSLLNKLIQAPNGRFTVINVDLNYFKQVNDSFGHAAGDAMLKAVAKRMQSVLR
CCCHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
NSDMVARLGGDEFLLILPRLTSPADISHVLMKVRHAACEQPLQMADITLNISISMGAARY
CCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCC
PEDGQDITELLHVADKAMYEDKQRIKGER
CCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQSTHLARTKLWIVAFAIVYWFLSLFTVEYLHRSYLRDYLAEQQQWFSQKLALIRANIEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
KVNADILLADSLATILSFNPDVPYSKWTLLAGQLASKGEYIRSISIAPNDVVALVYPLAG
ECCCCEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHEEEECCCCCEEEEEEEECC
NESVMGLDFRETPAQMPAVLEARMSRQITLDGPITLIQGGIGLIARAPVFADPPTNTQYW
CCCEEECCCCCCCHHHHHHHHHHHCCEEEECCCEEEEECCCHHHCCCCCCCCCCCCCCHH
GVCSIVIDLDRLINSIRDVDLGEGMTLGIRTLLYPDKRGPIFIGQSSDFDNARAVAQVHL
HHHHHHHHHHHHHHHHHCCCCCCCCEEHHHEEECCCCCCCEEEECCCCCCCHHHHHEEEE
LGATWEIALAEPAELRSWGATNVVRLIGYCVTLFIFLSFLMVSNAYRMARLVSLQDVLTG
ECCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LPNRRFAMSLLNKLIQAPNGRFTVINVDLNYFKQVNDSFGHAAGDAMLKAVAKRMQSVLR
CCCHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
NSDMVARLGGDEFLLILPRLTSPADISHVLMKVRHAACEQPLQMADITLNISISMGAARY
CCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCC
PEDGQDITELLHVADKAMYEDKQRIKGER
CCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]