Click here to switch to the map view.

The map label for this gene is hemB [H]

Identifier: 119776343

GI number: 119776343

Start: 3800987

End: 3801997

Strand: Direct

Name: hemB [H]

Synonym: Sama_3211

Alternate gene names: 119776343

Gene position: 3800987-3801997 (Clockwise)

Preceding gene: 119776342

Following gene: 119776344

Centisome position: 88.27

GC content: 57.76

Gene sequence:

>1011_bases
GTGAACATCATCACCAGTGCCTTCCCACAACGCAGAATGCGCCGTATGCGCAAACACGAGTTCAGCCGTCGCCTGATGGC
CGAAAATACCCTGACGGTTAACGACCTGATTTATCCCATGTTTGTGCTCGAAGGTAATCACCGCACCGAGCAAGTGGCCT
CCATGCCCGGCATCGAGCGCTACAGTATCGATCTGCTCCTCAAAGAAGCAGAAGAGCTGGTCAAACTGGGGATTCCACTG
ATTGCCCTCTTCCCCGTGACCCCAGCGGAAAAGAAAACCCTGCTGGCAGAAGAAGCCTATAACCCCGATGGTTTGGCGCA
GCGCGCCGTGCGTGCCCTCAAGCGTGAGTTTCCCCAGCTGGGTGTGATGACCGACGTGGCCCTGGACCCCTTCACCACCC
ACGGTCAGGACGGCATCATCGATGACACCGGCTATATCGTTAACGACATCACCACCGACATTCTGGTGAAGCAGGCTCTG
TCCCATGCCGAGGCCGGTGCCGACATTGTGGCTCCGTCCGACATGATGGACGGCCGTATCGGCGCTATCCGTAACGCGCT
GGAAGCCGCTGGCCACGTGAATACCCAAATCATGGCCTACTCGGCCAAGTATTCATCCAACTACTATGGCCCATTCCGTG
ACGCCGTCGGCTCTGCCGGCAACCTCAAGGGTGGCAATAAACACAGCTACCAGATGGACCCGGCCAACAGCGATGAGGCC
CTCCATGAAGTGGCACTGGACATCCAGGAAGGTGCCGACATGGTGATGGTCAAGCCCGGCATGCCCTATCTGGATATAGT
GCACAGGGTGAAAACTGAATTGGCCGTGCCCACCTTTGCCTATCAGGTCAGTGGGGAGTACGCCATGCACATGGCGGCCA
TTCAAAACGGCTGGCTGGCGGAAAAAGCCATAGTGATGGAGTCCCTGCTGTGCTTCAAGCGCGCCGGGGCCGACGGCATT
CTCACTTACTTTGCCAAGCGTGCCGCCCAGTGGCTTAACGAGCAGAAGTAA

Upstream 100 bases:

>100_bases
AGGCGGACAAGGCGCTCTATCAGGCCAAACACGACGGCCGTAACAGGGTGGGCCTTTTTCAGGACATACCCGTTCCCCAT
CCATAGCCCAGGAGACGATA

Downstream 100 bases:

>100_bases
TACTGCCTGCAGCCAAAAGCCACCCTGGGGTGGCTTTTTTGTTTTCAGCGCAAAAGGTGATCCAGTCCAAAGCCTGAGCC
CATCAGGCTTTCTTTCGGCT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MNIITSAFPQRRMRRMRKHEFSRRLMAENTLTVNDLIYPMFVLEGNHRTEQVASMPGIERYSIDLLLKEAEELVKLGIPL
IALFPVTPAEKKTLLAEEAYNPDGLAQRAVRALKREFPQLGVMTDVALDPFTTHGQDGIIDDTGYIVNDITTDILVKQAL
SHAEAGADIVAPSDMMDGRIGAIRNALEAAGHVNTQIMAYSAKYSSNYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLAEKAIVMESLLCFKRAGADGI
LTYFAKRAAQWLNEQK

Sequences:

>Translated_336_residues
MNIITSAFPQRRMRRMRKHEFSRRLMAENTLTVNDLIYPMFVLEGNHRTEQVASMPGIERYSIDLLLKEAEELVKLGIPL
IALFPVTPAEKKTLLAEEAYNPDGLAQRAVRALKREFPQLGVMTDVALDPFTTHGQDGIIDDTGYIVNDITTDILVKQAL
SHAEAGADIVAPSDMMDGRIGAIRNALEAAGHVNTQIMAYSAKYSSNYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLAEKAIVMESLLCFKRAGADGI
LTYFAKRAAQWLNEQK
>Mature_336_residues
MNIITSAFPQRRMRRMRKHEFSRRLMAENTLTVNDLIYPMFVLEGNHRTEQVASMPGIERYSIDLLLKEAEELVKLGIPL
IALFPVTPAEKKTLLAEEAYNPDGLAQRAVRALKREFPQLGVMTDVALDPFTTHGQDGIIDDTGYIVNDITTDILVKQAL
SHAEAGADIVAPSDMMDGRIGAIRNALEAAGHVNTQIMAYSAKYSSNYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLAEKAIVMESLLCFKRAGADGI
LTYFAKRAAQWLNEQK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=311, Percent_Identity=41.4790996784566, Blast_Score=234, Evalue=6e-62,
Organism=Escherichia coli, GI87081728, Length=315, Percent_Identity=44.4444444444444, Blast_Score=239, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6321398, Length=324, Percent_Identity=37.6543209876543, Blast_Score=200, Evalue=3e-52,
Organism=Drosophila melanogaster, GI21358291, Length=320, Percent_Identity=38.75, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37192; Mature: 37192

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIITSAFPQRRMRRMRKHEFSRRLMAENTLTVNDLIYPMFVLEGNHRTEQVASMPGIER
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEECCCHHHHHHHCCCCCH
YSIDLLLKEAEELVKLGIPLIALFPVTPAEKKTLLAEEAYNPDGLAQRAVRALKREFPQL
HHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
GVMTDVALDPFTTHGQDGIIDDTGYIVNDITTDILVKQALSHAEAGADIVAPSDMMDGRI
CCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHCCHH
GAIRNALEAAGHVNTQIMAYSAKYSSNYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCEECCCCCCHHH
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLA
HHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCHH
EKAIVMESLLCFKRAGADGILTYFAKRAAQWLNEQK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIITSAFPQRRMRRMRKHEFSRRLMAENTLTVNDLIYPMFVLEGNHRTEQVASMPGIER
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEECCCHHHHHHHCCCCCH
YSIDLLLKEAEELVKLGIPLIALFPVTPAEKKTLLAEEAYNPDGLAQRAVRALKREFPQL
HHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
GVMTDVALDPFTTHGQDGIIDDTGYIVNDITTDILVKQALSHAEAGADIVAPSDMMDGRI
CCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHCCHH
GAIRNALEAAGHVNTQIMAYSAKYSSNYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCEECCCCCCHHH
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLA
HHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCHH
EKAIVMESLLCFKRAGADGILTYFAKRAAQWLNEQK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]