Click here to switch to the map view.

The map label for this gene is rpfG [H]

Identifier: 119776243

GI number: 119776243

Start: 3686578

End: 3687564

Strand: Direct

Name: rpfG [H]

Synonym: Sama_3111

Alternate gene names: 119776243

Gene position: 3686578-3687564 (Clockwise)

Preceding gene: 119776242

Following gene: 119776244

Centisome position: 85.61

GC content: 57.35

Gene sequence:

>987_bases
ATGCAGGACCTCAAGCCCCTCATTCTGGTGGTAGACGACGAAGCGGCCAACTTAAGGGTGCTCAAACAGATACTGCAGGA
TGAGTATCAGCTGAGCTTTGCCAAAAGTGGCCAGGAGGCGCTGCGCCTGGTGGGGCAAAAGCGGCCTGATCTTATCCTGC
TTGATATCATGATGCCCGATATGACAGGGCTGGATCTGTGCAAACTGCTCAAGGCCAATCCCCAGAGCGCCGGTATTCCG
GTGATTTTTGTCACCGCCCTCAACGACGAGGCCGACGAGGCCACGGGCTTTGCCGCCGGTGCAGTGGATTACATCACCAA
GCCGGTGTCCAGCGCCATTGTCAGGGCACGGGTGCGGACTCACCTGTCGCTGGTGCAGGCCGAAGAGCTAAAGGTTACCC
GGCTTAAAATCATCCAGCGCCTCGGCCGCGCTGCCGAATTCAAAGATAATGAAACCGGCATGCACGTCATGCGCATGAGC
CACTATGCCAAGGTGCTCGCCCGTGCCTATGGCCTTGGCGAAAGTCATGCCGACATGTTGCTGCACGCAGCACCCATGCA
CGATATAGGTAAAATCGGCATCGCAGACCACATACTGCTCAAGCCCGGCAAACTCACCGAAGAAGAGTTCGAGGTGATGA
AAACCCATCCGCAAATCGGCGCGGATATCATAGGGGAAGATGACTCACTGCTGATGCAAATGGCCCGCACCGTGGCCCTC
ACCCACCACGAAAAATGGGACGGCAGCGGCTACCCCAGGGGTATTGCCGGTGAGGCCATTCCGGTAGAGGGGCGCATTGT
TGCCATCGCCGATGTGTTCGATGCCCTCACCAGCGACCGCCCCTACAAAAAGGCCTGGAGCATAGAAGACACACTACGCC
TGATGCGGGAGCAAAAAGGCCGCCACTTCGAGCCCCTGCTGGTGGAGCTGCTGGAGCAAAACCTCGACAAGATCCTCGAC
ATCAAACAAACCTTTGTGGACGAATAG

Upstream 100 bases:

>100_bases
AAATTTCGGCATCCTTGTCCTCATATCAGACAACAGCACAGTCAGCCAATAATCCGGCACAAGACGCGGCGCAATCGAAT
AACGGCACAAAGGAGCAATA

Downstream 100 bases:

>100_bases
CCCGGCCCCCCATCAGCCAAGGCGCAGCCCCTTAGTCTCAGTCGATTGGTGCAATGACTTTTACTGCTAGTCTGGGTTAA
AAATCCCACTCAAGATGGAG

Product: response regulator receiver protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MQDLKPLILVVDDEAANLRVLKQILQDEYQLSFAKSGQEALRLVGQKRPDLILLDIMMPDMTGLDLCKLLKANPQSAGIP
VIFVTALNDEADEATGFAAGAVDYITKPVSSAIVRARVRTHLSLVQAEELKVTRLKIIQRLGRAAEFKDNETGMHVMRMS
HYAKVLARAYGLGESHADMLLHAAPMHDIGKIGIADHILLKPGKLTEEEFEVMKTHPQIGADIIGEDDSLLMQMARTVAL
THHEKWDGSGYPRGIAGEAIPVEGRIVAIADVFDALTSDRPYKKAWSIEDTLRLMREQKGRHFEPLLVELLEQNLDKILD
IKQTFVDE

Sequences:

>Translated_328_residues
MQDLKPLILVVDDEAANLRVLKQILQDEYQLSFAKSGQEALRLVGQKRPDLILLDIMMPDMTGLDLCKLLKANPQSAGIP
VIFVTALNDEADEATGFAAGAVDYITKPVSSAIVRARVRTHLSLVQAEELKVTRLKIIQRLGRAAEFKDNETGMHVMRMS
HYAKVLARAYGLGESHADMLLHAAPMHDIGKIGIADHILLKPGKLTEEEFEVMKTHPQIGADIIGEDDSLLMQMARTVAL
THHEKWDGSGYPRGIAGEAIPVEGRIVAIADVFDALTSDRPYKKAWSIEDTLRLMREQKGRHFEPLLVELLEQNLDKILD
IKQTFVDE
>Mature_328_residues
MQDLKPLILVVDDEAANLRVLKQILQDEYQLSFAKSGQEALRLVGQKRPDLILLDIMMPDMTGLDLCKLLKANPQSAGIP
VIFVTALNDEADEATGFAAGAVDYITKPVSSAIVRARVRTHLSLVQAEELKVTRLKIIQRLGRAAEFKDNETGMHVMRMS
HYAKVLARAYGLGESHADMLLHAAPMHDIGKIGIADHILLKPGKLTEEEFEVMKTHPQIGADIIGEDDSLLMQMARTVAL
THHEKWDGSGYPRGIAGEAIPVEGRIVAIADVFDALTSDRPYKKAWSIEDTLRLMREQKGRHFEPLLVELLEQNLDKILD
IKQTFVDE

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=128, Percent_Identity=39.0625, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=38.9380530973451, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790860, Length=151, Percent_Identity=31.7880794701987, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789149, Length=103, Percent_Identity=37.8640776699029, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789809, Length=124, Percent_Identity=36.2903225806452, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786911, Length=116, Percent_Identity=34.4827586206897, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786784, Length=173, Percent_Identity=28.3236994219653, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790863, Length=119, Percent_Identity=33.6134453781513, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=36.5384615384615, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 36527; Mature: 36527

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLKPLILVVDDEAANLRVLKQILQDEYQLSFAKSGQEALRLVGQKRPDLILLDIMMPD
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEEEECCC
MTGLDLCKLLKANPQSAGIPVIFVTALNDEADEATGFAAGAVDYITKPVSSAIVRARVRT
CCHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
HLSLVQAEELKVTRLKIIQRLGRAAEFKDNETGMHVMRMSHYAKVLARAYGLGESHADML
HHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LHAAPMHDIGKIGIADHILLKPGKLTEEEFEVMKTHPQIGADIIGEDDSLLMQMARTVAL
HHHCCCHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHH
THHEKWDGSGYPRGIAGEAIPVEGRIVAIADVFDALTSDRPYKKAWSIEDTLRLMREQKG
HHCCCCCCCCCCCCCCCCEECCCCEEEHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHCC
RHFEPLLVELLEQNLDKILDIKQTFVDE
CCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQDLKPLILVVDDEAANLRVLKQILQDEYQLSFAKSGQEALRLVGQKRPDLILLDIMMPD
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEEEECCC
MTGLDLCKLLKANPQSAGIPVIFVTALNDEADEATGFAAGAVDYITKPVSSAIVRARVRT
CCHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
HLSLVQAEELKVTRLKIIQRLGRAAEFKDNETGMHVMRMSHYAKVLARAYGLGESHADML
HHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LHAAPMHDIGKIGIADHILLKPGKLTEEEFEVMKTHPQIGADIIGEDDSLLMQMARTVAL
HHHCCCHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHH
THHEKWDGSGYPRGIAGEAIPVEGRIVAIADVFDALTSDRPYKKAWSIEDTLRLMREQKG
HHCCCCCCCCCCCCCCCCEECCCCEEEHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHCC
RHFEPLLVELLEQNLDKILDIKQTFVDE
CCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]