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The map label for this gene is mnmC [H]

Identifier: 119776231

GI number: 119776231

Start: 3673331

End: 3674023

Strand: Reverse

Name: mnmC [H]

Synonym: Sama_3099

Alternate gene names: 119776231

Gene position: 3674023-3673331 (Counterclockwise)

Preceding gene: 119776232

Following gene: 119776228

Centisome position: 85.32

GC content: 54.83

Gene sequence:

>693_bases
ATGAATATCACGTTAGCACTCACTGGAGACGGCTCTCACACCCTGGTCAACAGCGTACTGGATGAAAGCTATCATGTTTT
CAAAGGGGCGCTGACCGAGTCTGTGTATGTTTACATAGATGCGGGTCTGATACCGATGGCCGCACTAAAGCCCTCGCTGA
GAATCCTTGAAGTGGGTTTTGGTACCGGGCTGAATGTGCTCCTGACCCGGCAAAAGGCCTGTGCGATGGGCCTGGATATC
CATCTCACGACCCTGGAGCCCTTCCCCCTGAATGATGAAATTGTCTCGGCACTGAATTATGTGGACTGTCTGGCTGAGCT
GGGCGGCTATGGTGATCGCGATACCCTTGCCAAATGCTTCCGCGAGCTGCATCGGGCCCCGTGGGGGCAGGACGTGGCAT
TACAGGGAAGCACACTTCATAAAATTCAGGGCAGGCTTGAGGAGCAGGTATTTTCTCCCGAACGCTTTGATCTGATTTAT
TTCGATGCCTTCGCCCCCGCGAAGCAACCCGAACTGTGGGCGGCTGAAAACTTCACCAAGCTGTTTAATGCACTGGCACC
CGAGGGCCTTTTGGTCAGCTACTGTGCCAATGGTCAGTTCAAGCGGGATTTGAAAGCGGCAGGGTTCAGGGTCGAGAGTT
ACCCAGGCCCTATGGGACGCAGGCACATGACCCGGGCCTGGAAGGGACTGTAA

Upstream 100 bases:

>100_bases
TTGCGTAGCCTCTGATTTCAGCCATTACGCCATGGAAATTGGTGGCGCGATTGCCTAAAATCCCCTGGTTTTTATCGTCA
ATAACCCAGTATTCCTTTCC

Downstream 100 bases:

>100_bases
CGGGGGAACACTGCGACATTAATGGGTGAACTTTTCCGCAAACTTCTCTGCGGGTATGGCCTTACCATACAGATAGCCCT
GGGCAAAATCACATTCCTCG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MNITLALTGDGSHTLVNSVLDESYHVFKGALTESVYVYIDAGLIPMAALKPSLRILEVGFGTGLNVLLTRQKACAMGLDI
HLTTLEPFPLNDEIVSALNYVDCLAELGGYGDRDTLAKCFRELHRAPWGQDVALQGSTLHKIQGRLEEQVFSPERFDLIY
FDAFAPAKQPELWAAENFTKLFNALAPEGLLVSYCANGQFKRDLKAAGFRVESYPGPMGRRHMTRAWKGL

Sequences:

>Translated_230_residues
MNITLALTGDGSHTLVNSVLDESYHVFKGALTESVYVYIDAGLIPMAALKPSLRILEVGFGTGLNVLLTRQKACAMGLDI
HLTTLEPFPLNDEIVSALNYVDCLAELGGYGDRDTLAKCFRELHRAPWGQDVALQGSTLHKIQGRLEEQVFSPERFDLIY
FDAFAPAKQPELWAAENFTKLFNALAPEGLLVSYCANGQFKRDLKAAGFRVESYPGPMGRRHMTRAWKGL
>Mature_230_residues
MNITLALTGDGSHTLVNSVLDESYHVFKGALTESVYVYIDAGLIPMAALKPSLRILEVGFGTGLNVLLTRQKACAMGLDI
HLTTLEPFPLNDEIVSALNYVDCLAELGGYGDRDTLAKCFRELHRAPWGQDVALQGSTLHKIQGRLEEQVFSPERFDLIY
FDAFAPAKQPELWAAENFTKLFNALAPEGLLVSYCANGQFKRDLKAAGFRVESYPGPMGRRHMTRAWKGL

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 25438; Mature: 25438

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITLALTGDGSHTLVNSVLDESYHVFKGALTESVYVYIDAGLIPMAALKPSLRILEVGF
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHCCCCCEEEEEEC
GTGLNVLLTRQKACAMGLDIHLTTLEPFPLNDEIVSALNYVDCLAELGGYGDRDTLAKCF
CCCCEEEEECHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RELHRAPWGQDVALQGSTLHKIQGRLEEQVFSPERFDLIYFDAFAPAKQPELWAAENFTK
HHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
LFNALAPEGLLVSYCANGQFKRDLKAAGFRVESYPGPMGRRHMTRAWKGL
HHHHHCCCCEEEEEECCCCHHHHHHHHCEEEECCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MNITLALTGDGSHTLVNSVLDESYHVFKGALTESVYVYIDAGLIPMAALKPSLRILEVGF
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHCCCCCEEEEEEC
GTGLNVLLTRQKACAMGLDIHLTTLEPFPLNDEIVSALNYVDCLAELGGYGDRDTLAKCF
CCCCEEEEECHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RELHRAPWGQDVALQGSTLHKIQGRLEEQVFSPERFDLIYFDAFAPAKQPELWAAENFTK
HHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
LFNALAPEGLLVSYCANGQFKRDLKAAGFRVESYPGPMGRRHMTRAWKGL
HHHHHCCCCEEEEEECCCCHHHHHHHHCEEEECCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA