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The map label for this gene is purA

Identifier: 119776201

GI number: 119776201

Start: 3647279

End: 3648574

Strand: Reverse

Name: purA

Synonym: Sama_3069

Alternate gene names: 119776201

Gene position: 3648574-3647279 (Counterclockwise)

Preceding gene: 119776202

Following gene: 119776200

Centisome position: 84.73

GC content: 52.78

Gene sequence:

>1296_bases
ATGGGCAAAAACGTAGTTGTTCTCGGCACCCAATGGGGTGACGAGGGAAAAGGTAAGATTGTCGACCTTCTCACTGAACA
GGCAAAATACGTAGTTCGTTATCAGGGCGGCCACAACGCTGGTCACACTCTGGTAATCGATGGTGTTAAAACCGTTCTCC
ATCTGATCCCCTCAGGCATCCTGCGCGATAATGTGAAATGTATTATCGGTAACGGTGTTGTGCTGGCTCCAGACGCGCTG
ATGAAAGAAATCAGCATGCTCAAAGAGCGTGGCGTACCCGTTGAAGATCGCCTGCTGATCTCCGAAGCTTGTCCACTTAT
CCTGCCGTTCCACTGTGCAGTGGATATCGCCCGTGAAAAGGCGCGCGGCAACAAGGCTATCGGTACCACTGGTCGTGGTA
TTGGCCCTGCCTATGAGGACAAGGTGTCACGTCGCGGTCTGCGCGTGGGAGATCTGTTCAATGCTGAACTCTTCGCCGAG
AAACTCAAAGAAGTGATGAAGTATCACAACTTCATGCTGACTGAGTACTATGGCGCCGAAGCCGTTGACTATCAGACAAC
CCTCGACGATGCACTGGCAATGGCCGACTACCTCAAGTCTATGTGCGTTGACGTGACAGAAATGCTGGATCAGGCCCGCA
AAGCCGGTGAAAACATACTGTTCGAAGGTGCTCAGGGTACACTGCTGGACATCGACCACGGTACTTATCCGTTCGTGACT
TCGTCCAACACCACTGCTGGCGGCGTTGCAACCGGAAGCGGTTTTGGTCCATGTCATCTGGACTATGTGCTGGGTATCAT
GAAGGCCTACACCACTCGCGTGGGTGCCGGTCCTTTCCCAACTGAACTCGAGTGTGAAATTGGTGACCATCTGGGTACCA
AGGGCCACGAGTTTGGTGCCACCACTGGCCGTAAGCGTCGTCCAGGTTGGCTCGATGCTGTTGCCATGAAGCGTGCTATC
CAGATCAACAGTCTGACAGGTATCTGTTTGACCAAGTTGGACGTGCTGGACGGCCTGGAAGAAGTGAAGATCTGTGTGGG
TTATCAGCAGCCAGATGGCACTGTTACCACTGTGACTCCTCTTGCCGCTGAAGGTTATGAGCTGGTAACCCCAGTTTATG
AAACCCTGCCAGGTTGGAGCGAGAACACCTTCGGTGTGACTTCCATGGATCAGCTGCCACAGGCGGCCGTTAATTACATC
AAACGTATCGAAGAGATTTTGGAGACGCCAATCGACATCATCTCTACCGGTCCGGATAGGAACGAGACCTTTATCCGGGT
CAGTCCGTTTAACTGA

Upstream 100 bases:

>100_bases
AGGCGTCAGGCGGCAATTGATTATTTTTTCATAAATCCCTTGCCCATCCCCCCCTGAAATCTGCTAAAATTCTGCTTTAA
CCGCAACCTTGCAGCAAACA

Downstream 100 bases:

>100_bases
TGTAAGAAGAGGCCGCGATTAGCGGCCTTTTTTATGTCTGTCTCTTGAGGGGACAGACTCAAGATTTGAGTTATACTGCC
GATAAATTGGTTATCTGCCA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGVKTVLHLIPSGILRDNVKCIIGNGVVLAPDAL
MKEISMLKERGVPVEDRLLISEACPLILPFHCAVDIAREKARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFNAELFAE
KLKEVMKYHNFMLTEYYGAEAVDYQTTLDDALAMADYLKSMCVDVTEMLDQARKAGENILFEGAQGTLLDIDHGTYPFVT
SSNTTAGGVATGSGFGPCHLDYVLGIMKAYTTRVGAGPFPTELECEIGDHLGTKGHEFGATTGRKRRPGWLDAVAMKRAI
QINSLTGICLTKLDVLDGLEEVKICVGYQQPDGTVTTVTPLAAEGYELVTPVYETLPGWSENTFGVTSMDQLPQAAVNYI
KRIEEILETPIDIISTGPDRNETFIRVSPFN

Sequences:

>Translated_431_residues
MGKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGVKTVLHLIPSGILRDNVKCIIGNGVVLAPDAL
MKEISMLKERGVPVEDRLLISEACPLILPFHCAVDIAREKARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFNAELFAE
KLKEVMKYHNFMLTEYYGAEAVDYQTTLDDALAMADYLKSMCVDVTEMLDQARKAGENILFEGAQGTLLDIDHGTYPFVT
SSNTTAGGVATGSGFGPCHLDYVLGIMKAYTTRVGAGPFPTELECEIGDHLGTKGHEFGATTGRKRRPGWLDAVAMKRAI
QINSLTGICLTKLDVLDGLEEVKICVGYQQPDGTVTTVTPLAAEGYELVTPVYETLPGWSENTFGVTSMDQLPQAAVNYI
KRIEEILETPIDIISTGPDRNETFIRVSPFN
>Mature_430_residues
GKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGVKTVLHLIPSGILRDNVKCIIGNGVVLAPDALM
KEISMLKERGVPVEDRLLISEACPLILPFHCAVDIAREKARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFNAELFAEK
LKEVMKYHNFMLTEYYGAEAVDYQTTLDDALAMADYLKSMCVDVTEMLDQARKAGENILFEGAQGTLLDIDHGTYPFVTS
SNTTAGGVATGSGFGPCHLDYVLGIMKAYTTRVGAGPFPTELECEIGDHLGTKGHEFGATTGRKRRPGWLDAVAMKRAIQ
INSLTGICLTKLDVLDGLEEVKICVGYQQPDGTVTTVTPLAAEGYELVTPVYETLPGWSENTFGVTSMDQLPQAAVNYIK
RIEEILETPIDIISTGPDRNETFIRVSPFN

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=44.4186046511628, Blast_Score=328, Evalue=9e-90,
Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=44.1588785046729, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=42.3558897243108, Blast_Score=283, Evalue=3e-76,
Organism=Escherichia coli, GI1790620, Length=431, Percent_Identity=74.7099767981439, Blast_Score=671, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146553, Length=434, Percent_Identity=42.6267281105991, Blast_Score=323, Evalue=8e-89,
Organism=Caenorhabditis elegans, GI25146555, Length=434, Percent_Identity=42.6267281105991, Blast_Score=323, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6324109, Length=423, Percent_Identity=43.4988179669031, Blast_Score=330, Evalue=2e-91,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=43.3962264150943, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_SHEAM (A1SA65)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928941.1
- ProteinModelPortal:   A1SA65
- SMR:   A1SA65
- STRING:   A1SA65
- GeneID:   4605316
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_3069
- NMPDR:   fig|326297.7.peg.2886
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   A1SA65
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46817; Mature: 46686

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGVKTVLHLIPSGI
CCCCEEEEEECCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCEEEEHHHHHHHHHHCCHH
LRDNVKCIIGNGVVLAPDALMKEISMLKERGVPVEDRLLISEACPLILPFHCAVDIAREK
HHCCCEEEEECCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHH
ARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFNAELFAEKLKEVMKYHNFMLTEYYGAE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCC
AVDYQTTLDDALAMADYLKSMCVDVTEMLDQARKAGENILFEGAQGTLLDIDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGFGPCHLDYVLGIMKAYTTRVGAGPFPTELECEIGDHLGTKGHEFGA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCC
TTGRKRRPGWLDAVAMKRAIQINSLTGICLTKLDVLDGLEEVKICVGYQQPDGTVTTVTP
CCCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHCCHHHEEEEEEEECCCCCEEEECC
LAAEGYELVTPVYETLPGWSENTFGVTSMDQLPQAAVNYIKRIEEILETPIDIISTGPDR
CCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCC
NETFIRVSPFN
CCEEEEEECCC
>Mature Secondary Structure 
GKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGVKTVLHLIPSGI
CCCEEEEEECCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCEEEEHHHHHHHHHHCCHH
LRDNVKCIIGNGVVLAPDALMKEISMLKERGVPVEDRLLISEACPLILPFHCAVDIAREK
HHCCCEEEEECCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHH
ARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFNAELFAEKLKEVMKYHNFMLTEYYGAE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCC
AVDYQTTLDDALAMADYLKSMCVDVTEMLDQARKAGENILFEGAQGTLLDIDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGFGPCHLDYVLGIMKAYTTRVGAGPFPTELECEIGDHLGTKGHEFGA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCC
TTGRKRRPGWLDAVAMKRAIQINSLTGICLTKLDVLDGLEEVKICVGYQQPDGTVTTVTP
CCCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHCCHHHEEEEEEEECCCCCEEEECC
LAAEGYELVTPVYETLPGWSENTFGVTSMDQLPQAAVNYIKRIEEILETPIDIISTGPDR
CCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCC
NETFIRVSPFN
CCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA