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The map label for this gene is napB [H]

Identifier: 119776118

GI number: 119776118

Start: 3557534

End: 3557965

Strand: Direct

Name: napB [H]

Synonym: Sama_2986

Alternate gene names: 119776118

Gene position: 3557534-3557965 (Clockwise)

Preceding gene: 119776117

Following gene: 119776120

Centisome position: 82.62

GC content: 57.87

Gene sequence:

>432_bases
ATGAAAAGAGTACTCACCTTAGCTGCGCTGTTTGTCGCCATCGCCGGTTGCAGCGGCCAGAACGCCCCGGTGAACAATGA
GCCGGTCAATGTCCAATCACTGGCGGGCAGCGCCCAGATCACTGACGTGCGCCCGGCCGATGCGCTGCCCACCTACCCCA
AGCGTGGCACCGCCATCGAGCGTAACTACAGTGAGCAGCCACCGCTTATTCCCCACAAGGACGACTACGCCATCACCTTA
AATAAAAATGGCTGTCTGAACTGTCACAGCTGGGATAAGGCCGAGCGGATGAAAGCCACCCCGGTGGCCAAGTCCCACAC
TGTGGATGCCAAGGGCACCCTCAATGGTCACAACTACTTCTGTACCCAGTGTCACGTGGCCCAGGCCGACAACAAGACCG
AGCTGGTGGAAAACACCTTCAGCACCAAATAA

Upstream 100 bases:

>100_bases
TGTGTGCCAAAGGCGTATTCCAGTACAAGAACCGATTTACATTCCAGGATAATCCTTTACAGAAAAGCCCCCGAATTGCC
AAGAAGGCGGAGAACGAACA

Downstream 100 bases:

>100_bases
GCCGGTTGCCATTAACAAAAAGCCCCGCAATCGCGGGGCTTTTTTCGTCAATACAAAGGTATGGATTAAAGGTGCTCAGG
CATCCTGCTGCCTGAGGCTC

Product: cytochrome c-type protein NapB

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MKRVLTLAALFVAIAGCSGQNAPVNNEPVNVQSLAGSAQITDVRPADALPTYPKRGTAIERNYSEQPPLIPHKDDYAITL
NKNGCLNCHSWDKAERMKATPVAKSHTVDAKGTLNGHNYFCTQCHVAQADNKTELVENTFSTK

Sequences:

>Translated_143_residues
MKRVLTLAALFVAIAGCSGQNAPVNNEPVNVQSLAGSAQITDVRPADALPTYPKRGTAIERNYSEQPPLIPHKDDYAITL
NKNGCLNCHSWDKAERMKATPVAKSHTVDAKGTLNGHNYFCTQCHVAQADNKTELVENTFSTK
>Mature_143_residues
MKRVLTLAALFVAIAGCSGQNAPVNNEPVNVQSLAGSAQITDVRPADALPTYPKRGTAIERNYSEQPPLIPHKDDYAITL
NKNGCLNCHSWDKAERMKATPVAKSHTVDAKGTLNGHNYFCTQCHVAQADNKTELVENTFSTK

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=94, Percent_Identity=38.2978723404255, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 15537; Mature: 15537

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVLTLAALFVAIAGCSGQNAPVNNEPVNVQSLAGSAQITDVRPADALPTYPKRGTAIE
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEECCCCCCCCCCCCCCCCEEC
RNYSEQPPLIPHKDDYAITLNKNGCLNCHSWDKAERMKATPVAKSHTVDAKGTLNGHNYF
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCEECCCCEE
CTQCHVAQADNKTELVENTFSTK
EEEEEEECCCCCHHHHHHHCCCC
>Mature Secondary Structure
MKRVLTLAALFVAIAGCSGQNAPVNNEPVNVQSLAGSAQITDVRPADALPTYPKRGTAIE
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEECCCCCCCCCCCCCCCCEEC
RNYSEQPPLIPHKDDYAITLNKNGCLNCHSWDKAERMKATPVAKSHTVDAKGTLNGHNYF
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCEECCCCEE
CTQCHVAQADNKTELVENTFSTK
EEEEEEECCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]