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The map label for this gene is napA [H]

Identifier: 119776115

GI number: 119776115

Start: 3553380

End: 3555863

Strand: Direct

Name: napA [H]

Synonym: Sama_2983

Alternate gene names: 119776115

Gene position: 3553380-3555863 (Clockwise)

Preceding gene: 119776114

Following gene: 119776116

Centisome position: 82.52

GC content: 58.21

Gene sequence:

>2484_bases
ATGAGCATAAGCCGTCGCGAGTTTCTTAAAGCCAATGCCGCCGTGGCTGCGGCCACGGCAGTGGGTGTCACTCTGCCAGT
GAAAGTGGTTGAAGCCGCTGAGCAGAAGGACAACATCAAATGGGACAAGGCCCCCTGCCGTTTCTGTGGCGTGGGCTGTT
CGGTGCTGGTTGGCACCCGGGACGGCAAAGTGGTTGCCACCAAGGGCGACCCCGAAAGCCCGGTCAACAAGGGCCTGAAC
TGTATCAAGGGCTACTTCCTGTCGAAAATCATGTACGGCAAAGACCGTCTGGACACGCCGCTTTTGCGCATGAAAGATGG
CAAGTACGACAAGAACGGCGAATTCACCCCGGTGAGCTGGGACGTGGCGCTGGACACCATGGCCGACAAGTGGAAAGCCA
GCCTCAAGGCCAAGGGTCCAACCTCTGTAGGTATGTTCGGATCGGGCCAGTGGACCATCTGGGAAGGCTATGCCGCCGCC
AAACTGCACAAGGCCGGTTTCCTCACCAATAACATCGACCCCAATGCCCGTCACTGCATGGCCTCGGCCGTAGGCGCCTT
TATGCGTACCTTCGGTATTGACGAGCCCATGGGCTGCTATGACGACCTCGAAGCCGCCGACCACTTTGTGCTCTGGGGCG
CCAACATGGCCGAGATGCACCCGATCCTGTGGGCGCGCCTGTCTGACCGTCGCCTGAGCAATCCCAACGGCAAGGTGCAT
GTGCTCTCTACCTTCGAGAACCGCTCCTTCGATCTGGCCGATAACCCCATGGTATTCCGTCCGCAAACGGACATGATTAT
CCTGAACTTTATCGCCAACTACATCATCCAGAACAAGGCGGTAAACAAGGACTTTGTCAGCAAGCACACCAACTTCGCTT
TGGGTGTGACCGACATAGGTTATGGTCTGCGCCCTGACCATCCGCTGGAGATGAAGGCCAAGAACCCAGGCAACGGCAAA
TCCACCCCGATTTCCTTCGACGAATACGCCAAGTTTGTCAGCACCTATACCCTGGAATACGCCAGCGAAATGAGTGGTGT
ACCGGCAGAGCGTTTGGTTGAGTTGGCCAAGGCCTATGCCGACCCAAGCGTGAAGGTGATGAGCCTGTGGACCATGGGGG
TGAACCAGCACGTGCGTGGTGTGTGGACCAACAACATGATCTACAACATCCACCTGCTGACCGGCAAAATCGCCACCCCG
GGCAACAGCCCCTTCTCACTCACCGGTCAGCCATCGGCCTGTGGTACCGCCCGTGAAGTGGGTACCTTTGCCCACCGTCT
GCCCGCCGACATGGTGGTGGCCAACGACAAGCACCGCGCCATTACCGAAAAACTCTGGCAGCTGCCGGAAGGCACCATTC
CGCCCAAGCCCGGCTTCCACGCCGTGCTGCAAAGCCGGATGCTCAAAGACGGCAAGCTGAACTGTTACTGGACCATGTGT
ACCAACAATATGCAGGCCGGTCCCAACATCAACGATGAAATCTACCCGGGCTTCCGTAACCCGGAAAACTTCATCGTGGT
ATCAGACCCTTACCCAACGGTGACTGCCATGGCCGCCGACCTGATTCTGCCTACCGCCATGTGGGTGGAAAAAGAAGGTG
CCTACGGTAACGCCGAGCGCCGTACCCATATGTGGCACCAGCAGGTGAAGGCACCGGAAGGCGCCAAGTCTGATCTGTGG
CAGCTGATGGAATTCGCCAAGCGCTTCACCGTGGATGAAGTCTGGCCCGCCGAGCTGCTGGCCAAGAAGCCAGAATACGC
CGGTAAGACCCTGTATCAGGTGCTGTTTGCCAACGGCGTTATCGACAAGTATCCAATCTCTGACTGCAAGGGCGAGCTGA
ACGATGAGTCCGAGCACTTCGGCTTCTACGTACAGAAGGGCATTTTCGAAGAATATGCCGCCTTCGGTCGCGGCAAGGCC
CACGATCTGGCAGCCTTCGACGACTACCACGCCAGCAGAGGTCTGCGCTGGCCAGTGGTGGATGGCAAGGAAACCCTGCG
CCGCTTCGTTGAGGGTTCCGACCCCTATGTGAAGGCCGGTGAAGGCTTTAAGTTCTACGGTAAGCCCGATGGCAAGGCGG
TGATTTTCGCCCTGCCTTACGAGCCAGCCGCCGAAGAGCCGAACGATGAATACGATCTGTGGATGAGCACAGGCCGGGTA
CTGGAACACTGGCACACCGCCTCCATGACAGCACGGGTACCTGAGCTCTACCGCGCTTACCCTGACGCGCAGATCTTTAT
GCACCCTGAAGACGCCAAGGCCCGTGGCCTGAACCGTGGCGACGAGGTAATAGTGGCCTCTCCACGGGGTGAAGTGAAGA
CACGTGTCGAAACCAAGGGCCGCAACAAGCCACCCCGTGGCGTGGTGTTTATGCCCTTCTTCGACGCCCGTCAGCTGGTG
AACAAGCTGCTGCTGGACGCGACCGATCCTCTGTCAAAAGAGACAGACTTCAAGAAGTGTCCGGTGAAGGTGATGAAGGC
CTGA

Upstream 100 bases:

>100_bases
TCGACAATGTCGAGGCCATCAATGCCCTTAACGGGGTGCTATCCAGCAGTCTGATCTATCACCAATCAGAGCAAGAACAA
CAAGTGAGTGAGGAAACACC

Downstream 100 bases:

>100_bases
TAAGGCCGGAATACGCCAAGGCCGTGAACGGGAGAGCACTATGATGACCAGACAACAGAGTAAGACCCAAGGCATTAACC
GGCGCCAATTTCTGGCGACG

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 827; Mature: 826

Protein sequence:

>827_residues
MSISRREFLKANAAVAAATAVGVTLPVKVVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTRDGKVVATKGDPESPVNKGLN
CIKGYFLSKIMYGKDRLDTPLLRMKDGKYDKNGEFTPVSWDVALDTMADKWKASLKAKGPTSVGMFGSGQWTIWEGYAAA
KLHKAGFLTNNIDPNARHCMASAVGAFMRTFGIDEPMGCYDDLEAADHFVLWGANMAEMHPILWARLSDRRLSNPNGKVH
VLSTFENRSFDLADNPMVFRPQTDMIILNFIANYIIQNKAVNKDFVSKHTNFALGVTDIGYGLRPDHPLEMKAKNPGNGK
STPISFDEYAKFVSTYTLEYASEMSGVPAERLVELAKAYADPSVKVMSLWTMGVNQHVRGVWTNNMIYNIHLLTGKIATP
GNSPFSLTGQPSACGTAREVGTFAHRLPADMVVANDKHRAITEKLWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMC
TNNMQAGPNINDEIYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGAKSDLW
QLMEFAKRFTVDEVWPAELLAKKPEYAGKTLYQVLFANGVIDKYPISDCKGELNDESEHFGFYVQKGIFEEYAAFGRGKA
HDLAAFDDYHASRGLRWPVVDGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNDEYDLWMSTGRV
LEHWHTASMTARVPELYRAYPDAQIFMHPEDAKARGLNRGDEVIVASPRGEVKTRVETKGRNKPPRGVVFMPFFDARQLV
NKLLLDATDPLSKETDFKKCPVKVMKA

Sequences:

>Translated_827_residues
MSISRREFLKANAAVAAATAVGVTLPVKVVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTRDGKVVATKGDPESPVNKGLN
CIKGYFLSKIMYGKDRLDTPLLRMKDGKYDKNGEFTPVSWDVALDTMADKWKASLKAKGPTSVGMFGSGQWTIWEGYAAA
KLHKAGFLTNNIDPNARHCMASAVGAFMRTFGIDEPMGCYDDLEAADHFVLWGANMAEMHPILWARLSDRRLSNPNGKVH
VLSTFENRSFDLADNPMVFRPQTDMIILNFIANYIIQNKAVNKDFVSKHTNFALGVTDIGYGLRPDHPLEMKAKNPGNGK
STPISFDEYAKFVSTYTLEYASEMSGVPAERLVELAKAYADPSVKVMSLWTMGVNQHVRGVWTNNMIYNIHLLTGKIATP
GNSPFSLTGQPSACGTAREVGTFAHRLPADMVVANDKHRAITEKLWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMC
TNNMQAGPNINDEIYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGAKSDLW
QLMEFAKRFTVDEVWPAELLAKKPEYAGKTLYQVLFANGVIDKYPISDCKGELNDESEHFGFYVQKGIFEEYAAFGRGKA
HDLAAFDDYHASRGLRWPVVDGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNDEYDLWMSTGRV
LEHWHTASMTARVPELYRAYPDAQIFMHPEDAKARGLNRGDEVIVASPRGEVKTRVETKGRNKPPRGVVFMPFFDARQLV
NKLLLDATDPLSKETDFKKCPVKVMKA
>Mature_826_residues
SISRREFLKANAAVAAATAVGVTLPVKVVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTRDGKVVATKGDPESPVNKGLNC
IKGYFLSKIMYGKDRLDTPLLRMKDGKYDKNGEFTPVSWDVALDTMADKWKASLKAKGPTSVGMFGSGQWTIWEGYAAAK
LHKAGFLTNNIDPNARHCMASAVGAFMRTFGIDEPMGCYDDLEAADHFVLWGANMAEMHPILWARLSDRRLSNPNGKVHV
LSTFENRSFDLADNPMVFRPQTDMIILNFIANYIIQNKAVNKDFVSKHTNFALGVTDIGYGLRPDHPLEMKAKNPGNGKS
TPISFDEYAKFVSTYTLEYASEMSGVPAERLVELAKAYADPSVKVMSLWTMGVNQHVRGVWTNNMIYNIHLLTGKIATPG
NSPFSLTGQPSACGTAREVGTFAHRLPADMVVANDKHRAITEKLWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMCT
NNMQAGPNINDEIYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGAKSDLWQ
LMEFAKRFTVDEVWPAELLAKKPEYAGKTLYQVLFANGVIDKYPISDCKGELNDESEHFGFYVQKGIFEEYAAFGRGKAH
DLAAFDDYHASRGLRWPVVDGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNDEYDLWMSTGRVL
EHWHTASMTARVPELYRAYPDAQIFMHPEDAKARGLNRGDEVIVASPRGEVKTRVETKGRNKPPRGVVFMPFFDARQLVN
KLLLDATDPLSKETDFKKCPVKVMKA

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=827, Percent_Identity=71.584038694075, Blast_Score=1268, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=799, Percent_Identity=24.5306633291614, Blast_Score=195, Evalue=1e-50,
Organism=Escherichia coli, GI3868720, Length=601, Percent_Identity=24.6256239600666, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI3868719, Length=488, Percent_Identity=25.2049180327869, Blast_Score=109, Evalue=9e-25,
Organism=Escherichia coli, GI1787870, Length=812, Percent_Identity=23.6453201970443, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI171474008, Length=833, Percent_Identity=23.8895558223289, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 92172; Mature: 92041

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISRREFLKANAAVAAATAVGVTLPVKVVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTR
CCCCHHHHHHHHHHHHHHHHHCEECHHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEECC
DGKVVATKGDPESPVNKGLNCIKGYFLSKIMYGKDRLDTPLLRMKDGKYDKNGEFTPVSW
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCEEEE
DVALDTMADKWKASLKAKGPTSVGMFGSGQWTIWEGYAAAKLHKAGFLTNNIDPNARHCM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEECCCCCCHHHHH
ASAVGAFMRTFGIDEPMGCYDDLEAADHFVLWGANMAEMHPILWARLSDRRLSNPNGKVH
HHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEE
VLSTFENRSFDLADNPMVFRPQTDMIILNFIANYIIQNKAVNKDFVSKHTNFALGVTDIG
EEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCC
YGLRPDHPLEMKAKNPGNGKSTPISFDEYAKFVSTYTLEYASEMSGVPAERLVELAKAYA
CCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPSVKVMSLWTMGVNQHVRGVWTNNMIYNIHLLTGKIATPGNSPFSLTGQPSACGTAREV
CCCEEEEEEEECCCCCCCCEEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHHHH
GTFAHRLPADMVVANDKHRAITEKLWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMC
HHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
TNNMQAGPNINDEIYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAER
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHH
RTHMWHQQVKAPEGAKSDLWQLMEFAKRFTVDEVWPAELLAKKPEYAGKTLYQVLFANGV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCC
IDKYPISDCKGELNDESEHFGFYVQKGIFEEYAAFGRGKAHDLAAFDDYHASRGLRWPVV
CCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
DGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNDEYDLWMSTGRV
CCHHHHHHHHCCCCCEEECCCCEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHH
LEHWHTASMTARVPELYRAYPDAQIFMHPEDAKARGLNRGDEVIVASPRGEVKTRVETKG
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHCCCCCCCEEEEECCCCCCEEEHHHCC
RNKPPRGVVFMPFFDARQLVNKLLLDATDPLSKETDFKKCPVKVMKA
CCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
SISRREFLKANAAVAAATAVGVTLPVKVVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTR
CCCHHHHHHHHHHHHHHHHHCEECHHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEECC
DGKVVATKGDPESPVNKGLNCIKGYFLSKIMYGKDRLDTPLLRMKDGKYDKNGEFTPVSW
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCEEEE
DVALDTMADKWKASLKAKGPTSVGMFGSGQWTIWEGYAAAKLHKAGFLTNNIDPNARHCM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEECCCCCCHHHHH
ASAVGAFMRTFGIDEPMGCYDDLEAADHFVLWGANMAEMHPILWARLSDRRLSNPNGKVH
HHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEE
VLSTFENRSFDLADNPMVFRPQTDMIILNFIANYIIQNKAVNKDFVSKHTNFALGVTDIG
EEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCC
YGLRPDHPLEMKAKNPGNGKSTPISFDEYAKFVSTYTLEYASEMSGVPAERLVELAKAYA
CCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPSVKVMSLWTMGVNQHVRGVWTNNMIYNIHLLTGKIATPGNSPFSLTGQPSACGTAREV
CCCEEEEEEEECCCCCCCCEEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHHHH
GTFAHRLPADMVVANDKHRAITEKLWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMC
HHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
TNNMQAGPNINDEIYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAER
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHH
RTHMWHQQVKAPEGAKSDLWQLMEFAKRFTVDEVWPAELLAKKPEYAGKTLYQVLFANGV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCC
IDKYPISDCKGELNDESEHFGFYVQKGIFEEYAAFGRGKAHDLAAFDDYHASRGLRWPVV
CCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
DGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNDEYDLWMSTGRV
CCHHHHHHHHCCCCCEEECCCCEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHH
LEHWHTASMTARVPELYRAYPDAQIFMHPEDAKARGLNRGDEVIVASPRGEVKTRVETKG
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHCCCCCCCEEEEECCCCCCEEEHHHCC
RNKPPRGVVFMPFFDARQLVNKLLLDATDPLSKETDFKKCPVKVMKA
CCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]